Differential transcription of steroidogenic enzymes in the equine primary corpus luteum during diestrus and early pregnancy
In pregnant mares, eCG stimulates luteal androgen and estrogen production, increasing plasma concentrations 2- to 3-fold. To study how these changes are regulated, we examined the expression of mRMA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), cytochrome P450 17 al...
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| creator | Albrecht, B.A MacLeod, J.N Daels, P.F |
| description | In pregnant mares, eCG stimulates luteal androgen and estrogen production, increasing plasma concentrations 2- to 3-fold. To study how these changes are regulated, we examined the expression of mRMA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), cytochrome P450 17 alpha-hydroxylase/17,20-lyase (P450(17 alpha)), and cytochrome P450 aromatase (P450arom) in equine primary corpora lutea using Northern blot analyses. Three equine specific cDNAs were generated by reverse transcriptase polymerase chain reaction. When compared to human, bovine, and rat sequences, the nucleotide identities were 82%, 84%, and 76%, respectively, for 3 beta-HSD cDNA (843 base pairs [bp]); 79%, 80% and 66% for P450(17 alpha) cDNA (541 bp); and 80%, 83% and 75% for P450arom cDNA (289 bp). The P450(17 alpha) cDNA sequence demonstrated 99.6% nucleotide identity with the previously published sequence for equine testicular P450(17 alpha). Luteal tissue samples were collected at three times: diestrus (Days 8-10), early pregnancy before the onset of eCG secretion (Days 29-35), and early pregnancy after the onset of eCG secretion (Days 42-45). Although no significant changes were observed in 3 beta-HSD expression, P450(17 alpha) and P450arom demonstrated stage-specific transcriptional regulation. Steady-state levels of P450(17 alpha) mRNA were similar during diestrus and early pregnancy before the onset of eCG secretion but increased significantly after the onset of eCG secretion. Cytochrome P450arom mRNA levels decreased significantly after the onset of eCG secretion. Steady-state levels of P450arom mRNA were highest in luteal tissue collected during pregnancy before the onset of eCG secretion and intermediate during diestrus. Secretion of eCG appears to increase luteal estrogen synthesis by a transcriptional up-regulation of P450(17 alpha), expression |
| doi_str_mv | 10.1095/biolreprod56.4.821 |
| format | Article |
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To study how these changes are regulated, we examined the expression of mRMA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), cytochrome P450 17 alpha-hydroxylase/17,20-lyase (P450(17 alpha)), and cytochrome P450 aromatase (P450arom) in equine primary corpora lutea using Northern blot analyses. Three equine specific cDNAs were generated by reverse transcriptase polymerase chain reaction. When compared to human, bovine, and rat sequences, the nucleotide identities were 82%, 84%, and 76%, respectively, for 3 beta-HSD cDNA (843 base pairs [bp]); 79%, 80% and 66% for P450(17 alpha) cDNA (541 bp); and 80%, 83% and 75% for P450arom cDNA (289 bp). The P450(17 alpha) cDNA sequence demonstrated 99.6% nucleotide identity with the previously published sequence for equine testicular P450(17 alpha). Luteal tissue samples were collected at three times: diestrus (Days 8-10), early pregnancy before the onset of eCG secretion (Days 29-35), and early pregnancy after the onset of eCG secretion (Days 42-45). Although no significant changes were observed in 3 beta-HSD expression, P450(17 alpha) and P450arom demonstrated stage-specific transcriptional regulation. Steady-state levels of P450(17 alpha) mRNA were similar during diestrus and early pregnancy before the onset of eCG secretion but increased significantly after the onset of eCG secretion. Cytochrome P450arom mRNA levels decreased significantly after the onset of eCG secretion. Steady-state levels of P450arom mRNA were highest in luteal tissue collected during pregnancy before the onset of eCG secretion and intermediate during diestrus. Secretion of eCG appears to increase luteal estrogen synthesis by a transcriptional up-regulation of P450(17 alpha), expression</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod56.4.821</identifier><identifier>PMID: 9096861</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>3-Hydroxysteroid Dehydrogenases - biosynthesis ; 3-Hydroxysteroid Dehydrogenases - genetics ; ADN ; Animals ; ARN MENSAJERO ; ARN MESSAGER ; Aromatase - biosynthesis ; Aromatase - genetics ; Base Sequence ; Biological and medical sciences ; CABALLOS ; Cattle ; CHEVAL ; CHORIONIC GONADOTROPIN ; CICLO ESTRAL ; CITOCROMO P450 ; COMPLEMENTARY DNA ; CORPS JAUNE ; CORPUS LUTEUM ; Corpus Luteum - enzymology ; CUERPO LUTEO ; CYCLE OESTRAL ; CYTOCHROME P450 ; Diestrus - metabolism ; DNA ; DNA Primers ; DNA Probes ; DNA, Complementary ; EQUINE CHORIONIC FONADOTROPIN ; Female ; Fundamental and applied biological sciences. Psychology ; GESTACION ; GESTATION ; GONADOTROPHINE ; GONADOTROPINAS ; GONADOTROPINS ; HORMONAS ; HORMONE ; Hormone metabolism and regulation ; HORMONES ; HORSES ; Humans ; HYDROXYSTEROID DEHYDROGENASE ; MESSENGER RNA ; METABOLISME DES STEROIDES ; METABOLISMO DE ESTEROIDES ; MOLECULAR SEQUENCE DATA ; NUCLEOTIDE SEQUENCE ; OESTROUS CYCLE ; OXIDOREDUCTASES ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; OXYGENASES ; Polymerase Chain Reaction ; PREGNANCY ; Pregnancy, Animal - metabolism ; Pregnancy. Parturition. Lactation ; Rats ; REGULATION ; SECRECION ; SECRETION ; SECUENCIA NUCLEOTIDICA ; Sequence Homology, Nucleic Acid ; SEQUENCE NUCLEOTIDIQUE ; Steroid 17-alpha-Hydroxylase - biosynthesis ; Steroid 17-alpha-Hydroxylase - genetics ; STEROID METABOLISM ; STEROIDOGENESIS ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription, Genetic ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1997-04, Vol.56 (4), p.821-829</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2640183$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9096861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Albrecht, B.A</creatorcontrib><creatorcontrib>MacLeod, J.N</creatorcontrib><creatorcontrib>Daels, P.F</creatorcontrib><title>Differential transcription of steroidogenic enzymes in the equine primary corpus luteum during diestrus and early pregnancy</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>In pregnant mares, eCG stimulates luteal androgen and estrogen production, increasing plasma concentrations 2- to 3-fold. To study how these changes are regulated, we examined the expression of mRMA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), cytochrome P450 17 alpha-hydroxylase/17,20-lyase (P450(17 alpha)), and cytochrome P450 aromatase (P450arom) in equine primary corpora lutea using Northern blot analyses. Three equine specific cDNAs were generated by reverse transcriptase polymerase chain reaction. When compared to human, bovine, and rat sequences, the nucleotide identities were 82%, 84%, and 76%, respectively, for 3 beta-HSD cDNA (843 base pairs [bp]); 79%, 80% and 66% for P450(17 alpha) cDNA (541 bp); and 80%, 83% and 75% for P450arom cDNA (289 bp). The P450(17 alpha) cDNA sequence demonstrated 99.6% nucleotide identity with the previously published sequence for equine testicular P450(17 alpha). Luteal tissue samples were collected at three times: diestrus (Days 8-10), early pregnancy before the onset of eCG secretion (Days 29-35), and early pregnancy after the onset of eCG secretion (Days 42-45). Although no significant changes were observed in 3 beta-HSD expression, P450(17 alpha) and P450arom demonstrated stage-specific transcriptional regulation. Steady-state levels of P450(17 alpha) mRNA were similar during diestrus and early pregnancy before the onset of eCG secretion but increased significantly after the onset of eCG secretion. Cytochrome P450arom mRNA levels decreased significantly after the onset of eCG secretion. Steady-state levels of P450arom mRNA were highest in luteal tissue collected during pregnancy before the onset of eCG secretion and intermediate during diestrus. Secretion of eCG appears to increase luteal estrogen synthesis by a transcriptional up-regulation of P450(17 alpha), expression</description><subject>3-Hydroxysteroid Dehydrogenases - biosynthesis</subject><subject>3-Hydroxysteroid Dehydrogenases - genetics</subject><subject>ADN</subject><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Aromatase - biosynthesis</subject><subject>Aromatase - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CABALLOS</subject><subject>Cattle</subject><subject>CHEVAL</subject><subject>CHORIONIC GONADOTROPIN</subject><subject>CICLO ESTRAL</subject><subject>CITOCROMO P450</subject><subject>COMPLEMENTARY DNA</subject><subject>CORPS JAUNE</subject><subject>CORPUS LUTEUM</subject><subject>Corpus Luteum - enzymology</subject><subject>CUERPO LUTEO</subject><subject>CYCLE OESTRAL</subject><subject>CYTOCHROME P450</subject><subject>Diestrus - metabolism</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA Probes</subject><subject>DNA, Complementary</subject><subject>EQUINE CHORIONIC FONADOTROPIN</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GESTACION</subject><subject>GESTATION</subject><subject>GONADOTROPHINE</subject><subject>GONADOTROPINAS</subject><subject>GONADOTROPINS</subject><subject>HORMONAS</subject><subject>HORMONE</subject><subject>Hormone metabolism and regulation</subject><subject>HORMONES</subject><subject>HORSES</subject><subject>Humans</subject><subject>HYDROXYSTEROID DEHYDROGENASE</subject><subject>MESSENGER RNA</subject><subject>METABOLISME DES STEROIDES</subject><subject>METABOLISMO DE ESTEROIDES</subject><subject>MOLECULAR SEQUENCE DATA</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>OESTROUS CYCLE</subject><subject>OXIDOREDUCTASES</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>OXYGENASES</subject><subject>Polymerase Chain Reaction</subject><subject>PREGNANCY</subject><subject>Pregnancy, Animal - metabolism</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Rats</subject><subject>REGULATION</subject><subject>SECRECION</subject><subject>SECRETION</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Steroid 17-alpha-Hydroxylase - biosynthesis</subject><subject>Steroid 17-alpha-Hydroxylase - genetics</subject><subject>STEROID METABOLISM</subject><subject>STEROIDOGENESIS</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhhtR1nH1DwhCDuqtx3x35yjrJyx40D03NUmlJ5JOzybdDKN_3sA2evRUVL0PbxVvNc1LRveMGvXuEOaY8ZRnp_Re7nvOHjU7prhpO677x82OUqpbIbR42jwr5SelTAourporQ43uNds1vz8E7zFjWgJEsmRIxeZwWsKcyOxJWTDPwc0jpmAJpl-XCQsJiSxHJHi_hoTklMME-ULsnE9rIXFdcJ2IW3NII3EBy5LrGJIjCDleKo9jgmQvz5snHmLBF1u9bu4-ffxx86W9_fb5683729YL1i9tZ7nnTqNnxqFVIDstutrZnnstwWjjDrbXVGkNSJUyCMCRASjee6mcuG7ePvjWpO7Xes8whWIxRkg4r2XoeiO54PS_INNSaWV4BV9t4HqY0A1bBMMWa9VfbzoUC9HXWG0ofzGuJWW9qNibB-wYxuM5ZBzKBDFWUzGcz2elBznUp_5b52EeYMzV6u47M6ajykhBxR-KWaI1</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>Albrecht, B.A</creator><creator>MacLeod, J.N</creator><creator>Daels, P.F</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Differential transcription of steroidogenic enzymes in the equine primary corpus luteum during diestrus and early pregnancy</title><author>Albrecht, B.A ; MacLeod, J.N ; Daels, P.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f318t-7c2f2d6ef19dec5a47637ef1c82f64a969dbc860566ae0559eaa2e1aa528f45d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>3-Hydroxysteroid Dehydrogenases - biosynthesis</topic><topic>3-Hydroxysteroid Dehydrogenases - genetics</topic><topic>ADN</topic><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Aromatase - biosynthesis</topic><topic>Aromatase - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CABALLOS</topic><topic>Cattle</topic><topic>CHEVAL</topic><topic>CHORIONIC GONADOTROPIN</topic><topic>CICLO ESTRAL</topic><topic>CITOCROMO P450</topic><topic>COMPLEMENTARY DNA</topic><topic>CORPS JAUNE</topic><topic>CORPUS LUTEUM</topic><topic>Corpus Luteum - enzymology</topic><topic>CUERPO LUTEO</topic><topic>CYCLE OESTRAL</topic><topic>CYTOCHROME P450</topic><topic>Diestrus - metabolism</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA Probes</topic><topic>DNA, Complementary</topic><topic>EQUINE CHORIONIC FONADOTROPIN</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GESTACION</topic><topic>GESTATION</topic><topic>GONADOTROPHINE</topic><topic>GONADOTROPINAS</topic><topic>GONADOTROPINS</topic><topic>HORMONAS</topic><topic>HORMONE</topic><topic>Hormone metabolism and regulation</topic><topic>HORMONES</topic><topic>HORSES</topic><topic>Humans</topic><topic>HYDROXYSTEROID DEHYDROGENASE</topic><topic>MESSENGER RNA</topic><topic>METABOLISME DES STEROIDES</topic><topic>METABOLISMO DE ESTEROIDES</topic><topic>MOLECULAR SEQUENCE DATA</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>OESTROUS CYCLE</topic><topic>OXIDOREDUCTASES</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>OXYGENASES</topic><topic>Polymerase Chain Reaction</topic><topic>PREGNANCY</topic><topic>Pregnancy, Animal - metabolism</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Rats</topic><topic>REGULATION</topic><topic>SECRECION</topic><topic>SECRETION</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Steroid 17-alpha-Hydroxylase - biosynthesis</topic><topic>Steroid 17-alpha-Hydroxylase - genetics</topic><topic>STEROID METABOLISM</topic><topic>STEROIDOGENESIS</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albrecht, B.A</creatorcontrib><creatorcontrib>MacLeod, J.N</creatorcontrib><creatorcontrib>Daels, P.F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albrecht, B.A</au><au>MacLeod, J.N</au><au>Daels, P.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential transcription of steroidogenic enzymes in the equine primary corpus luteum during diestrus and early pregnancy</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>56</volume><issue>4</issue><spage>821</spage><epage>829</epage><pages>821-829</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>In pregnant mares, eCG stimulates luteal androgen and estrogen production, increasing plasma concentrations 2- to 3-fold. To study how these changes are regulated, we examined the expression of mRMA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), cytochrome P450 17 alpha-hydroxylase/17,20-lyase (P450(17 alpha)), and cytochrome P450 aromatase (P450arom) in equine primary corpora lutea using Northern blot analyses. Three equine specific cDNAs were generated by reverse transcriptase polymerase chain reaction. When compared to human, bovine, and rat sequences, the nucleotide identities were 82%, 84%, and 76%, respectively, for 3 beta-HSD cDNA (843 base pairs [bp]); 79%, 80% and 66% for P450(17 alpha) cDNA (541 bp); and 80%, 83% and 75% for P450arom cDNA (289 bp). The P450(17 alpha) cDNA sequence demonstrated 99.6% nucleotide identity with the previously published sequence for equine testicular P450(17 alpha). Luteal tissue samples were collected at three times: diestrus (Days 8-10), early pregnancy before the onset of eCG secretion (Days 29-35), and early pregnancy after the onset of eCG secretion (Days 42-45). Although no significant changes were observed in 3 beta-HSD expression, P450(17 alpha) and P450arom demonstrated stage-specific transcriptional regulation. Steady-state levels of P450(17 alpha) mRNA were similar during diestrus and early pregnancy before the onset of eCG secretion but increased significantly after the onset of eCG secretion. Cytochrome P450arom mRNA levels decreased significantly after the onset of eCG secretion. Steady-state levels of P450arom mRNA were highest in luteal tissue collected during pregnancy before the onset of eCG secretion and intermediate during diestrus. Secretion of eCG appears to increase luteal estrogen synthesis by a transcriptional up-regulation of P450(17 alpha), expression</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9096861</pmid><doi>10.1095/biolreprod56.4.821</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 1997-04, Vol.56 (4), p.821-829 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | 3-Hydroxysteroid Dehydrogenases - biosynthesis 3-Hydroxysteroid Dehydrogenases - genetics ADN Animals ARN MENSAJERO ARN MESSAGER Aromatase - biosynthesis Aromatase - genetics Base Sequence Biological and medical sciences CABALLOS Cattle CHEVAL CHORIONIC GONADOTROPIN CICLO ESTRAL CITOCROMO P450 COMPLEMENTARY DNA CORPS JAUNE CORPUS LUTEUM Corpus Luteum - enzymology CUERPO LUTEO CYCLE OESTRAL CYTOCHROME P450 Diestrus - metabolism DNA DNA Primers DNA Probes DNA, Complementary EQUINE CHORIONIC FONADOTROPIN Female Fundamental and applied biological sciences. Psychology GESTACION GESTATION GONADOTROPHINE GONADOTROPINAS GONADOTROPINS HORMONAS HORMONE Hormone metabolism and regulation HORMONES HORSES Humans HYDROXYSTEROID DEHYDROGENASE MESSENGER RNA METABOLISME DES STEROIDES METABOLISMO DE ESTEROIDES MOLECULAR SEQUENCE DATA NUCLEOTIDE SEQUENCE OESTROUS CYCLE OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE OXYGENASES Polymerase Chain Reaction PREGNANCY Pregnancy, Animal - metabolism Pregnancy. Parturition. Lactation Rats REGULATION SECRECION SECRETION SECUENCIA NUCLEOTIDICA Sequence Homology, Nucleic Acid SEQUENCE NUCLEOTIDIQUE Steroid 17-alpha-Hydroxylase - biosynthesis Steroid 17-alpha-Hydroxylase - genetics STEROID METABOLISM STEROIDOGENESIS TRANSCRIPCION TRANSCRIPTION Transcription, Genetic Vertebrates: reproduction |
| title | Differential transcription of steroidogenic enzymes in the equine primary corpus luteum during diestrus and early pregnancy |
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