Nuclear localisation of calreticulin in vivo is enhanced by its interaction with glucocorticoid receptors
The multi-functional protein calreticulin (CRT) is normally found within the lumen of the endoplasmic reticulum (ER). However, some of its proposed functions require it to be located within the nucleus, where its presence is contentious. We have investigated this in live COS7, HeLa and LM(TK−) cells...
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| Veröffentlicht in: | FEBS letters 1997-03, Vol.405 (2), p.181-185 |
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| creator | Roderick, H.Llewelyn Campbell, Anthony K Llewellyn, David H |
| description | The multi-functional protein calreticulin (CRT) is normally found within the lumen of the endoplasmic reticulum (ER). However, some of its proposed functions require it to be located within the nucleus, where its presence is contentious. We have investigated this in live COS7, HeLa and LM(TK−) cells using green fluorescent protein (GFP)-fusion proteins. GFP-CRT, and GFP, with an ER signal peptide and a KDEL sequence (ER-GFP), were localised to the ER. In addition, GFP-CRT was located in the nucleus of all the cell types at low levels. The higher levels of nuclear fluorescence in LM(TK−) and HeLa cells suggested that glucocorticoid receptors might enhance nuclear localisation of calreticulin. Dexamethasone treatment of LM(TK−) cells doubled the amount of nuclear GFP-CRT, but did not affect the localisation of a GFP-CRT fusion in which the glucocorticoid receptor-binding N-domain of calreticulin had been deleted. Thus, despite ER targeting and retention signals, calreticulin is also located within the nucleus where its presence increases due to its interaction with glucocorticoid receptors.
© 1997 Federation of European Biochemical Societies. |
| doi_str_mv | 10.1016/S0014-5793(97)00183-X |
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© 1997 Federation of European Biochemical Societies.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(97)00183-X</identifier><identifier>PMID: 9089287</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - metabolism ; Calreticulin ; Cell Compartmentation ; Cell Nucleus - metabolism ; Confocal microscopy ; COS Cells ; Dexamethasone - pharmacology ; Endoplasmic reticulum ; Endoplasmic Reticulum - metabolism ; GFP (green fluorescent protein) ; Glucocorticoid receptor ; Glucocorticoids - pharmacology ; Green Fluorescent Proteins ; HeLa Cells ; Humans ; Immunohistochemistry ; Luminescent Proteins - genetics ; Nucleus ; Oligopeptides - genetics ; Oligopeptides - metabolism ; Protein Sorting Signals - genetics ; Protein Sorting Signals - metabolism ; Receptors, Glucocorticoid - metabolism ; Recombinant Fusion Proteins - genetics ; Ribonucleoproteins - genetics ; Ribonucleoproteins - metabolism</subject><ispartof>FEBS letters, 1997-03, Vol.405 (2), p.181-185</ispartof><rights>1997 Federation of European Biochemical Societies</rights><rights>FEBS Letters 405 (1997) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c448X-ce9ac2c40212f76abcd314876604cc6f47502bb4490c05fb8e760668517d7f2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2897%2900183-X$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S001457939700183X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9089287$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roderick, H.Llewelyn</creatorcontrib><creatorcontrib>Campbell, Anthony K</creatorcontrib><creatorcontrib>Llewellyn, David H</creatorcontrib><title>Nuclear localisation of calreticulin in vivo is enhanced by its interaction with glucocorticoid receptors</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The multi-functional protein calreticulin (CRT) is normally found within the lumen of the endoplasmic reticulum (ER). However, some of its proposed functions require it to be located within the nucleus, where its presence is contentious. We have investigated this in live COS7, HeLa and LM(TK−) cells using green fluorescent protein (GFP)-fusion proteins. GFP-CRT, and GFP, with an ER signal peptide and a KDEL sequence (ER-GFP), were localised to the ER. In addition, GFP-CRT was located in the nucleus of all the cell types at low levels. The higher levels of nuclear fluorescence in LM(TK−) and HeLa cells suggested that glucocorticoid receptors might enhance nuclear localisation of calreticulin. Dexamethasone treatment of LM(TK−) cells doubled the amount of nuclear GFP-CRT, but did not affect the localisation of a GFP-CRT fusion in which the glucocorticoid receptor-binding N-domain of calreticulin had been deleted. Thus, despite ER targeting and retention signals, calreticulin is also located within the nucleus where its presence increases due to its interaction with glucocorticoid receptors.
© 1997 Federation of European Biochemical Societies.</description><subject>Animals</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Calreticulin</subject><subject>Cell Compartmentation</subject><subject>Cell Nucleus - metabolism</subject><subject>Confocal microscopy</subject><subject>COS Cells</subject><subject>Dexamethasone - pharmacology</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>GFP (green fluorescent protein)</subject><subject>Glucocorticoid receptor</subject><subject>Glucocorticoids - pharmacology</subject><subject>Green Fluorescent Proteins</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Luminescent Proteins - genetics</subject><subject>Nucleus</subject><subject>Oligopeptides - genetics</subject><subject>Oligopeptides - metabolism</subject><subject>Protein Sorting Signals - genetics</subject><subject>Protein Sorting Signals - metabolism</subject><subject>Receptors, Glucocorticoid - metabolism</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Ribonucleoproteins - genetics</subject><subject>Ribonucleoproteins - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtq3DAUhkVpSSdpHyGgVWkXTiVb1mVV2pBLISSLtjA7IR8fNyoeayrZE-btI3uGbFsQiKP_csRHyDlnF5xx-fkHY1wUtTLVR6M-5UFXxfoVWXGtqqISUr8mqxfLW3Ka0h82u7g5ISeGaVNqtSL-foIeXaR9ANf75EYfBho6mqeIo4ep9wPNZ-d3gfpEcXh0A2BLmz31Y8rSiNHBEnvy4yP93U8QIMScDb6lEQG3Y4jpHXnTuT7h--N9Rn5dX_28vC3uHm6-X369K0AIvS4AjYMSBCt52SnpGmgrLrSSkgkA2QlVs7JphDAMWN01GpVkUuqaq1Z1ZVudkQ-H3m0MfydMo934BNj3bsAwJau0EWXJdDbWByPEkFLEzm6j37i4t5zZGbFdENuZnzXKLojtOufOjwumZoPtS-rINOu3B_3J97j_v1J7ffWtXJRZMGp5nld9OVRhBrbzGG0CjzN-n7mOtg3-H599BpvQoYw</recordid><startdate>19970324</startdate><enddate>19970324</enddate><creator>Roderick, H.Llewelyn</creator><creator>Campbell, Anthony K</creator><creator>Llewellyn, David H</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970324</creationdate><title>Nuclear localisation of calreticulin in vivo is enhanced by its interaction with glucocorticoid receptors</title><author>Roderick, H.Llewelyn ; Campbell, Anthony K ; Llewellyn, David H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448X-ce9ac2c40212f76abcd314876604cc6f47502bb4490c05fb8e760668517d7f2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Calreticulin</topic><topic>Cell Compartmentation</topic><topic>Cell Nucleus - metabolism</topic><topic>Confocal microscopy</topic><topic>COS Cells</topic><topic>Dexamethasone - pharmacology</topic><topic>Endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>GFP (green fluorescent protein)</topic><topic>Glucocorticoid receptor</topic><topic>Glucocorticoids - pharmacology</topic><topic>Green Fluorescent Proteins</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Luminescent Proteins - genetics</topic><topic>Nucleus</topic><topic>Oligopeptides - genetics</topic><topic>Oligopeptides - metabolism</topic><topic>Protein Sorting Signals - genetics</topic><topic>Protein Sorting Signals - metabolism</topic><topic>Receptors, Glucocorticoid - metabolism</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Ribonucleoproteins - genetics</topic><topic>Ribonucleoproteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roderick, H.Llewelyn</creatorcontrib><creatorcontrib>Campbell, Anthony K</creatorcontrib><creatorcontrib>Llewellyn, David H</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roderick, H.Llewelyn</au><au>Campbell, Anthony K</au><au>Llewellyn, David H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear localisation of calreticulin in vivo is enhanced by its interaction with glucocorticoid receptors</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-03-24</date><risdate>1997</risdate><volume>405</volume><issue>2</issue><spage>181</spage><epage>185</epage><pages>181-185</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The multi-functional protein calreticulin (CRT) is normally found within the lumen of the endoplasmic reticulum (ER). However, some of its proposed functions require it to be located within the nucleus, where its presence is contentious. We have investigated this in live COS7, HeLa and LM(TK−) cells using green fluorescent protein (GFP)-fusion proteins. GFP-CRT, and GFP, with an ER signal peptide and a KDEL sequence (ER-GFP), were localised to the ER. In addition, GFP-CRT was located in the nucleus of all the cell types at low levels. The higher levels of nuclear fluorescence in LM(TK−) and HeLa cells suggested that glucocorticoid receptors might enhance nuclear localisation of calreticulin. Dexamethasone treatment of LM(TK−) cells doubled the amount of nuclear GFP-CRT, but did not affect the localisation of a GFP-CRT fusion in which the glucocorticoid receptor-binding N-domain of calreticulin had been deleted. Thus, despite ER targeting and retention signals, calreticulin is also located within the nucleus where its presence increases due to its interaction with glucocorticoid receptors.
© 1997 Federation of European Biochemical Societies.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9089287</pmid><doi>10.1016/S0014-5793(97)00183-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism Calreticulin Cell Compartmentation Cell Nucleus - metabolism Confocal microscopy COS Cells Dexamethasone - pharmacology Endoplasmic reticulum Endoplasmic Reticulum - metabolism GFP (green fluorescent protein) Glucocorticoid receptor Glucocorticoids - pharmacology Green Fluorescent Proteins HeLa Cells Humans Immunohistochemistry Luminescent Proteins - genetics Nucleus Oligopeptides - genetics Oligopeptides - metabolism Protein Sorting Signals - genetics Protein Sorting Signals - metabolism Receptors, Glucocorticoid - metabolism Recombinant Fusion Proteins - genetics Ribonucleoproteins - genetics Ribonucleoproteins - metabolism |
| title | Nuclear localisation of calreticulin in vivo is enhanced by its interaction with glucocorticoid receptors |
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