Characterization of Chlorophyll a and Bacteriochlorophyll a Synthases by Heterologous Expression in Escherichia coli

Genes coding for putative chlorophyll a synthase ( chlG ) from Synechocystis sp. PCC 6803 and bacteriochlorophyll a synthase ( bchG ) from Rhodobacter capsulatus were amplified by the polymerase chain reaction and cloned into T7 RNA polymerase-based expression plasmids. In vitro enzymatic assays ind...

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Veröffentlicht in:	The Journal of biological chemistry 1997-04, Vol.272 (15), p.9671-9676
Hauptverfasser:	Oster, U, Bauer, C E, Rüdiger, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriochlorophylls - biosynthesis Carbon-Oxygen Ligases Chlorophyll - biosynthesis Chlorophyll A Chromatography, High Pressure Liquid Escherichia coli Ligases - genetics Ligases - metabolism Pigments, Biological - biosynthesis Rhodobacter capsulatus Spectrophotometry, Ultraviolet Synechocystis
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