Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes
A Streptomyces pristinaespiralis strain, which produces a streptogramin antibiotic pristinamycin II (PII) as a mixture of two biologically active molecules PIIB and PIIA, was genetically engineered to produce exclusively PIIA. The snaA,B genes, which encode a PIIA synthase that performs oxidation of...
Gespeichert in:
| Veröffentlicht in: | Nature biotechnology 1997-04, Vol.15 (4), p.349-353 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 353 |
|---|---|
| container_issue | 4 |
| container_start_page | 349 |
| container_title | Nature biotechnology |
| container_volume | 15 |
| creator | SEZONOV, G BLANC, V BAMAS-JACQUES, N FRIEDMANN, A PERNODET, J.-L GUERINEAU, M |
| description | A Streptomyces pristinaespiralis strain, which produces a streptogramin antibiotic pristinamycin II (PII) as a mixture of two biologically active molecules PIIB and PIIA, was genetically engineered to produce exclusively PIIA. The snaA,B genes, which encode a PIIA synthase that performs oxidation of the precursor (PIIB) to the final product (PIIA), were integrated in the chromosome of S. pristinaespiralis using an integrative derivative of the pSAM2 genetic element from Streptomyces ambofaciens. Integration was due to site-specific recombination at the attB site of S. pristinaespiralis, and no homologous recombination at the snaA,B locus was observed. The attB site of S. pristinaespiralis was sequenced and found to overlap the 3' end of a pro-tRNA gene. The integrants were stable in industrial conditions of pristinamycin production and showed no decrease in PII biosynthesis. Western blot analysis showed a constant production of the PIIA synthase in the overall fermentation process due to expression of the cloned snaA,B genes from the constitutive ermE promoter. This allows the complete conversion of the PIIB form into PIIA. |
| doi_str_mv | 10.1038/nbt0497-349 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78934950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78934950</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-fec8391156e86bc0584cdbaf3ece8889a6a08bb9a66ef0526be0b23aa4fe29863</originalsourceid><addsrcrecordid>eNo9kE9LJDEQxYOsqKuePC_ksHhZWtOddCY5yuCfgYE9qOcmidWapTtpU5nF-QR-bSO2c6oq6vcevEfIWc0uasbVZbCZCb2ouNB75KhuhaxqqeWPsjO1qFjdykPyE_EfY0wKKQ_IgWZa1FwekfdlHKcBMlAXw39I6GOgsacmZG99zN7RKYHbJIyJ5lgOj9kHM26dD3S1uqJ2S2MRwlvh8Ft-nxNMORYKcKcBnHwyg0danHEb8gt8-j9DADwh-70ZEE7neUweb64flnfV-u_tanm1rhxf6Fz14BTXdUkESlrHWiXckzU9BwdKKW2kYcraMiX0rG2kBWYbbozoodFK8mNy_uU7pfi6Aczd6NHBMJgAcYPdQulSY8sK-OcLdCkiJui7EmM0advVrPusvZtr7wpf6F-z7caO8LRj557L__f8N-jM0CcTnMcd1shWNFzwD4Fnj8Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78934950</pqid></control><display><type>article</type><title>Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Nature Journals Online</source><creator>SEZONOV, G ; BLANC, V ; BAMAS-JACQUES, N ; FRIEDMANN, A ; PERNODET, J.-L ; GUERINEAU, M</creator><creatorcontrib>SEZONOV, G ; BLANC, V ; BAMAS-JACQUES, N ; FRIEDMANN, A ; PERNODET, J.-L ; GUERINEAU, M</creatorcontrib><description>A Streptomyces pristinaespiralis strain, which produces a streptogramin antibiotic pristinamycin II (PII) as a mixture of two biologically active molecules PIIB and PIIA, was genetically engineered to produce exclusively PIIA. The snaA,B genes, which encode a PIIA synthase that performs oxidation of the precursor (PIIB) to the final product (PIIA), were integrated in the chromosome of S. pristinaespiralis using an integrative derivative of the pSAM2 genetic element from Streptomyces ambofaciens. Integration was due to site-specific recombination at the attB site of S. pristinaespiralis, and no homologous recombination at the snaA,B locus was observed. The attB site of S. pristinaespiralis was sequenced and found to overlap the 3' end of a pro-tRNA gene. The integrants were stable in industrial conditions of pristinamycin production and showed no decrease in PII biosynthesis. Western blot analysis showed a constant production of the PIIA synthase in the overall fermentation process due to expression of the cloned snaA,B genes from the constitutive ermE promoter. This allows the complete conversion of the PIIB form into PIIA.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0497-349</identifier><identifier>PMID: 9094136</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York, NY: Nature</publisher><subject>Attachment Sites, Microbiological - genetics ; Base Sequence ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, Bacterial ; Genetic Vectors ; Methods. Procedures. Technologies ; Molecular Sequence Data ; Nucleic Acid Conformation ; Recombination, Genetic ; Streptomyces - genetics ; Streptomyces - metabolism ; Virginiamycin - biosynthesis ; Virginiamycin - chemistry</subject><ispartof>Nature biotechnology, 1997-04, Vol.15 (4), p.349-353</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-fec8391156e86bc0584cdbaf3ece8889a6a08bb9a66ef0526be0b23aa4fe29863</citedby><cites>FETCH-LOGICAL-c379t-fec8391156e86bc0584cdbaf3ece8889a6a08bb9a66ef0526be0b23aa4fe29863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2654234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9094136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEZONOV, G</creatorcontrib><creatorcontrib>BLANC, V</creatorcontrib><creatorcontrib>BAMAS-JACQUES, N</creatorcontrib><creatorcontrib>FRIEDMANN, A</creatorcontrib><creatorcontrib>PERNODET, J.-L</creatorcontrib><creatorcontrib>GUERINEAU, M</creatorcontrib><title>Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><description>A Streptomyces pristinaespiralis strain, which produces a streptogramin antibiotic pristinamycin II (PII) as a mixture of two biologically active molecules PIIB and PIIA, was genetically engineered to produce exclusively PIIA. The snaA,B genes, which encode a PIIA synthase that performs oxidation of the precursor (PIIB) to the final product (PIIA), were integrated in the chromosome of S. pristinaespiralis using an integrative derivative of the pSAM2 genetic element from Streptomyces ambofaciens. Integration was due to site-specific recombination at the attB site of S. pristinaespiralis, and no homologous recombination at the snaA,B locus was observed. The attB site of S. pristinaespiralis was sequenced and found to overlap the 3' end of a pro-tRNA gene. The integrants were stable in industrial conditions of pristinamycin production and showed no decrease in PII biosynthesis. Western blot analysis showed a constant production of the PIIA synthase in the overall fermentation process due to expression of the cloned snaA,B genes from the constitutive ermE promoter. This allows the complete conversion of the PIIB form into PIIA.</description><subject>Attachment Sites, Microbiological - genetics</subject><subject>Base Sequence</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, Bacterial</subject><subject>Genetic Vectors</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Recombination, Genetic</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Virginiamycin - biosynthesis</subject><subject>Virginiamycin - chemistry</subject><issn>1087-0156</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9LJDEQxYOsqKuePC_ksHhZWtOddCY5yuCfgYE9qOcmidWapTtpU5nF-QR-bSO2c6oq6vcevEfIWc0uasbVZbCZCb2ouNB75KhuhaxqqeWPsjO1qFjdykPyE_EfY0wKKQ_IgWZa1FwekfdlHKcBMlAXw39I6GOgsacmZG99zN7RKYHbJIyJ5lgOj9kHM26dD3S1uqJ2S2MRwlvh8Ft-nxNMORYKcKcBnHwyg0danHEb8gt8-j9DADwh-70ZEE7neUweb64flnfV-u_tanm1rhxf6Fz14BTXdUkESlrHWiXckzU9BwdKKW2kYcraMiX0rG2kBWYbbozoodFK8mNy_uU7pfi6Aczd6NHBMJgAcYPdQulSY8sK-OcLdCkiJui7EmM0advVrPusvZtr7wpf6F-z7caO8LRj557L__f8N-jM0CcTnMcd1shWNFzwD4Fnj8Q</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>SEZONOV, G</creator><creator>BLANC, V</creator><creator>BAMAS-JACQUES, N</creator><creator>FRIEDMANN, A</creator><creator>PERNODET, J.-L</creator><creator>GUERINEAU, M</creator><general>Nature</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes</title><author>SEZONOV, G ; BLANC, V ; BAMAS-JACQUES, N ; FRIEDMANN, A ; PERNODET, J.-L ; GUERINEAU, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-fec8391156e86bc0584cdbaf3ece8889a6a08bb9a66ef0526be0b23aa4fe29863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Attachment Sites, Microbiological - genetics</topic><topic>Base Sequence</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, Bacterial</topic><topic>Genetic Vectors</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Recombination, Genetic</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Virginiamycin - biosynthesis</topic><topic>Virginiamycin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEZONOV, G</creatorcontrib><creatorcontrib>BLANC, V</creatorcontrib><creatorcontrib>BAMAS-JACQUES, N</creatorcontrib><creatorcontrib>FRIEDMANN, A</creatorcontrib><creatorcontrib>PERNODET, J.-L</creatorcontrib><creatorcontrib>GUERINEAU, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEZONOV, G</au><au>BLANC, V</au><au>BAMAS-JACQUES, N</au><au>FRIEDMANN, A</au><au>PERNODET, J.-L</au><au>GUERINEAU, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes</atitle><jtitle>Nature biotechnology</jtitle><addtitle>Nat Biotechnol</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>15</volume><issue>4</issue><spage>349</spage><epage>353</epage><pages>349-353</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><coden>NABIF9</coden><abstract>A Streptomyces pristinaespiralis strain, which produces a streptogramin antibiotic pristinamycin II (PII) as a mixture of two biologically active molecules PIIB and PIIA, was genetically engineered to produce exclusively PIIA. The snaA,B genes, which encode a PIIA synthase that performs oxidation of the precursor (PIIB) to the final product (PIIA), were integrated in the chromosome of S. pristinaespiralis using an integrative derivative of the pSAM2 genetic element from Streptomyces ambofaciens. Integration was due to site-specific recombination at the attB site of S. pristinaespiralis, and no homologous recombination at the snaA,B locus was observed. The attB site of S. pristinaespiralis was sequenced and found to overlap the 3' end of a pro-tRNA gene. The integrants were stable in industrial conditions of pristinamycin production and showed no decrease in PII biosynthesis. Western blot analysis showed a constant production of the PIIA synthase in the overall fermentation process due to expression of the cloned snaA,B genes from the constitutive ermE promoter. This allows the complete conversion of the PIIB form into PIIA.</abstract><cop>New York, NY</cop><pub>Nature</pub><pmid>9094136</pmid><doi>10.1038/nbt0497-349</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-0156 |
| ispartof | Nature biotechnology, 1997-04, Vol.15 (4), p.349-353 |
| issn | 1087-0156 1546-1696 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78934950 |
| source | MEDLINE; SpringerLink Journals; Nature Journals Online |
| subjects | Attachment Sites, Microbiological - genetics Base Sequence Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Cloning, Molecular DNA, Bacterial - chemistry DNA, Bacterial - genetics Fermentation Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Genetic Vectors Methods. Procedures. Technologies Molecular Sequence Data Nucleic Acid Conformation Recombination, Genetic Streptomyces - genetics Streptomyces - metabolism Virginiamycin - biosynthesis Virginiamycin - chemistry |
| title | Complete conversion of antibiotic precursor to pristinamycin IIA by overexpression of Streptomyces pristinaespiralis biosynthetic genes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T08%3A56%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Complete%20conversion%20of%20antibiotic%20precursor%20to%20pristinamycin%20IIA%20by%20overexpression%20of%20Streptomyces%20pristinaespiralis%20biosynthetic%20genes&rft.jtitle=Nature%20biotechnology&rft.au=SEZONOV,%20G&rft.date=1997-04-01&rft.volume=15&rft.issue=4&rft.spage=349&rft.epage=353&rft.pages=349-353&rft.issn=1087-0156&rft.eissn=1546-1696&rft.coden=NABIF9&rft_id=info:doi/10.1038/nbt0497-349&rft_dat=%3Cproquest_cross%3E78934950%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78934950&rft_id=info:pmid/9094136&rfr_iscdi=true |