High resolution autoantibody detection by optimized protein G-horseradish peroxidase immunostaining in a Western blotting assay

We report a procedure for optimisation of Western blotting using protein G-horseradish peroxidase (protein G-HRP) which avoids the false positive reactions often caused by second antibodies and increases the detection of autoantibodies by protein G conjugate. A number of modifications were investiga...

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