Constitutive activation of mitogen-activated protein kinase pathway in acute leukemia cells
Mitogen-activated protein (MAP) kinase appears to be one of the key regulators of cell proliferation and differentiation. Very little, however, has been revealed as to how MAP kinase is involved in leukemogenesis. We have studied the activation of the MAP kinase pathway in 100 human primary leukemia...
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| Veröffentlicht in: | Leukemia 1997-04, Vol.11 (4), p.479-484 |
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| creator | TOWATARI, M LIDA, H TANIMOTO, M IWATA, H HAMAGUCHI, M SAITO, H |
| description | Mitogen-activated protein (MAP) kinase appears to be one of the key regulators of cell proliferation and differentiation. Very little, however, has been revealed as to how MAP kinase is involved in leukemogenesis. We have studied the activation of the MAP kinase pathway in 100 human primary leukemia cells including 73 acute myelogenous leukemias (AMLs). Forty acute leukemia samples (40% of the total), including 37 AML samples (51% of AML), showed activation of MAP kinase as revealed by the mobility shift of the phosphorylated form of the protein and by in vitro kinase assay. This activation was correlated with MAP kinase kinase activity in these cells. In contrast, none of 14 chronic myelogenous leukemia samples showed the activation of MAP kinase. These results suggest that the MAP kinase pathway is constitutively activated in a subset of primary acute leukemias, and thus indicate the possible role of the constitutively activated MAP kinase in leukemogenesis. |
| doi_str_mv | 10.1038/sj.leu.2400617 |
| format | Article |
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Very little, however, has been revealed as to how MAP kinase is involved in leukemogenesis. We have studied the activation of the MAP kinase pathway in 100 human primary leukemia cells including 73 acute myelogenous leukemias (AMLs). Forty acute leukemia samples (40% of the total), including 37 AML samples (51% of AML), showed activation of MAP kinase as revealed by the mobility shift of the phosphorylated form of the protein and by in vitro kinase assay. This activation was correlated with MAP kinase kinase activity in these cells. In contrast, none of 14 chronic myelogenous leukemia samples showed the activation of MAP kinase. These results suggest that the MAP kinase pathway is constitutively activated in a subset of primary acute leukemias, and thus indicate the possible role of the constitutively activated MAP kinase in leukemogenesis.</description><identifier>ISSN: 0887-6924</identifier><identifier>EISSN: 1476-5551</identifier><identifier>DOI: 10.1038/sj.leu.2400617</identifier><identifier>PMID: 9096686</identifier><identifier>CODEN: LEUKED</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Acute myeloid leukemia ; Biological and medical sciences ; Calcium-Calmodulin-Dependent Protein Kinases - metabolism ; Cell differentiation ; Cell proliferation ; Chronic myeloid leukemia ; Electrophoretic mobility ; Enzyme Activation ; Genes, ras - physiology ; Hematologic and hematopoietic diseases ; Humans ; Kinases ; Leukemia ; Leukemia - enzymology ; Leukemia, Myelomonocytic, Acute - enzymology ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Leukemogenesis ; MAP kinase ; MAP kinase kinase ; Medical sciences ; Mitogen-Activated Protein Kinase 1 ; Mitogen-Activated Protein Kinase 3 ; Mitogen-Activated Protein Kinase Kinases ; Mitogen-Activated Protein Kinases ; Phosphorylation ; Protein kinase ; Protein Kinases - genetics ; Protein Kinases - physiology ; Proteins ; Tumor Cells, Cultured</subject><ispartof>Leukemia, 1997-04, Vol.11 (4), p.479-484</ispartof><rights>1997 INIST-CNRS</rights><rights>Macmillan Publishers Limited 1997.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-88e7c59e49107505d217dd515a904f8e376e52af7911e036b4f01805c7421b483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2633199$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9096686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TOWATARI, M</creatorcontrib><creatorcontrib>LIDA, H</creatorcontrib><creatorcontrib>TANIMOTO, M</creatorcontrib><creatorcontrib>IWATA, H</creatorcontrib><creatorcontrib>HAMAGUCHI, M</creatorcontrib><creatorcontrib>SAITO, H</creatorcontrib><title>Constitutive activation of mitogen-activated protein kinase pathway in acute leukemia cells</title><title>Leukemia</title><addtitle>Leukemia</addtitle><description>Mitogen-activated protein (MAP) kinase appears to be one of the key regulators of cell proliferation and differentiation. Very little, however, has been revealed as to how MAP kinase is involved in leukemogenesis. We have studied the activation of the MAP kinase pathway in 100 human primary leukemia cells including 73 acute myelogenous leukemias (AMLs). Forty acute leukemia samples (40% of the total), including 37 AML samples (51% of AML), showed activation of MAP kinase as revealed by the mobility shift of the phosphorylated form of the protein and by in vitro kinase assay. This activation was correlated with MAP kinase kinase activity in these cells. In contrast, none of 14 chronic myelogenous leukemia samples showed the activation of MAP kinase. These results suggest that the MAP kinase pathway is constitutively activated in a subset of primary acute leukemias, and thus indicate the possible role of the constitutively activated MAP kinase in leukemogenesis.</description><subject>Acute myeloid leukemia</subject><subject>Biological and medical sciences</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Chronic myeloid leukemia</subject><subject>Electrophoretic mobility</subject><subject>Enzyme Activation</subject><subject>Genes, ras - physiology</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Kinases</subject><subject>Leukemia</subject><subject>Leukemia - enzymology</subject><subject>Leukemia, Myelomonocytic, Acute - enzymology</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Leukemogenesis</subject><subject>MAP kinase</subject><subject>MAP kinase kinase</subject><subject>Medical sciences</subject><subject>Mitogen-Activated Protein Kinase 1</subject><subject>Mitogen-Activated Protein Kinase 3</subject><subject>Mitogen-Activated Protein Kinase Kinases</subject><subject>Mitogen-Activated Protein Kinases</subject><subject>Phosphorylation</subject><subject>Protein kinase</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - physiology</subject><subject>Proteins</subject><subject>Tumor Cells, Cultured</subject><issn>0887-6924</issn><issn>1476-5551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtrHDEQxoWJcc6PNl1AkOBuN3o_ynDETuAgjV25EDrtrK3zPi4rrc3999HhxUWaVAPz_Wbmkz6EPlFSU8LNt7SrO5hrJghRVJ-gFRVaVVJK-gGtiDG6UpaJj-g8pR0hR1GdoTNLrFJGrdDDehxSjnnO8QWwD6X4HMcBjy3uYx4fYaiWLjR4P40Z4oCf4-AT4L3PT6_-gEvHhzkDLk6eoY8eB-i6dIlOW98luFrqBbq_-XG3_lltft_-Wn_fVEEYkStjQAdpQVhKtCSyYVQ3jaTSWyJaA1wrkMy32lIKhKutaAk1RAYtGN0Kwy_Q9dveYu_PDCm7PqajAz_AOCenjeXcEPpfkEorLdOigF_-AXfjPA3lEY4pIZUt51mh6jcqTGNKE7RuP8XeTwdHiTuG49LOlS9xSzhl4POydt720LzjSxpF_7roPgXftZMfQkzvGFOcU2v5X2qYl1A</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>TOWATARI, M</creator><creator>LIDA, H</creator><creator>TANIMOTO, M</creator><creator>IWATA, H</creator><creator>HAMAGUCHI, M</creator><creator>SAITO, H</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Constitutive activation of mitogen-activated protein kinase pathway in acute leukemia cells</title><author>TOWATARI, M ; LIDA, H ; TANIMOTO, M ; IWATA, H ; HAMAGUCHI, M ; SAITO, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-88e7c59e49107505d217dd515a904f8e376e52af7911e036b4f01805c7421b483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Acute myeloid leukemia</topic><topic>Biological and medical sciences</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Chronic myeloid leukemia</topic><topic>Electrophoretic mobility</topic><topic>Enzyme Activation</topic><topic>Genes, ras - physiology</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Kinases</topic><topic>Leukemia</topic><topic>Leukemia - enzymology</topic><topic>Leukemia, Myelomonocytic, Acute - enzymology</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. 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| subjects | Acute myeloid leukemia Biological and medical sciences Calcium-Calmodulin-Dependent Protein Kinases - metabolism Cell differentiation Cell proliferation Chronic myeloid leukemia Electrophoretic mobility Enzyme Activation Genes, ras - physiology Hematologic and hematopoietic diseases Humans Kinases Leukemia Leukemia - enzymology Leukemia, Myelomonocytic, Acute - enzymology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Leukemogenesis MAP kinase MAP kinase kinase Medical sciences Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Phosphorylation Protein kinase Protein Kinases - genetics Protein Kinases - physiology Proteins Tumor Cells, Cultured |
| title | Constitutive activation of mitogen-activated protein kinase pathway in acute leukemia cells |
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