Nitric oxide mediates intracytoplasmic and intranuclear zinc release
We previously described that NO · leads to destruction of ZnS clusters and release of Zn 2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO · leads to an increase of cytoplasmic and nuclear free Zn 2+....
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| Veröffentlicht in: | FEBS letters 1997-03, Vol.405 (1), p.37-41 |
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| creator | Berendji, Denise Kolb-Bachofen, Victoria Meyer, Klaus L. Grapenthin, Olaf Weber, Horst Wahn, Volker Kröncke, Klaus-Dietrich |
| description | We previously described that NO
· leads to destruction of ZnS clusters and release of Zn
2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO
· leads to an increase of cytoplasmic and nuclear free Zn
2+. L929 cells, mouse splenocytes, or rat aorta endothelial cells were labeled with Zinquin-E, a Zn
2+-specific fluorophore, and were treated with two different spontaneous NO donors, S-nitroso-cysteine or DETA/NO. Both NO donors strongly increased the Zn
2+-dependent fluorescence in the cellular cytosol and also in nuclei as compared to controls. NO-dependent Zn
2+ release in splenocytes was quantitated by flow cytometry. These results show for the first time, that nitrosative stress mediates intracellular and intranuclear Zn
2+ release which may be relevant in altering gene expression patterns.
© 1997 Federation of European Biochemical Societies. |
| doi_str_mv | 10.1016/S0014-5793(97)00150-6 |
| format | Article |
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· leads to destruction of ZnS clusters and release of Zn
2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO
· leads to an increase of cytoplasmic and nuclear free Zn
2+. L929 cells, mouse splenocytes, or rat aorta endothelial cells were labeled with Zinquin-E, a Zn
2+-specific fluorophore, and were treated with two different spontaneous NO donors, S-nitroso-cysteine or DETA/NO. Both NO donors strongly increased the Zn
2+-dependent fluorescence in the cellular cytosol and also in nuclei as compared to controls. NO-dependent Zn
2+ release in splenocytes was quantitated by flow cytometry. These results show for the first time, that nitrosative stress mediates intracellular and intranuclear Zn
2+ release which may be relevant in altering gene expression patterns.
© 1997 Federation of European Biochemical Societies.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(97)00150-6</identifier><identifier>PMID: 9094420</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Aorta - cytology ; Cell Line ; Cell Nucleus - metabolism ; Cells, Cultured ; Cysteine - analogs & derivatives ; Cysteine - pharmacology ; Cytoplasm - metabolism ; Endothelium, Vascular - cytology ; Flow cytometry ; Fluorescence microscopy ; Fluorescent Dyes - chemistry ; Mice ; Nitric oxide ; Nitric Oxide - metabolism ; Quinolones - chemistry ; Rats ; S-Nitrosothiols ; Spleen - cytology ; Tosyl Compounds - chemistry ; Triazenes - pharmacology ; Zinc ; Zinc - metabolism ; Zinquin</subject><ispartof>FEBS letters, 1997-03, Vol.405 (1), p.37-41</ispartof><rights>1997 Federation of European Biochemical Societies</rights><rights>FEBS Letters 405 (1997) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5906-681f5b90d15596a17370870c07b7c31f19fa06ad4d619b5ed347ae96c23ee35c3</citedby><cites>FETCH-LOGICAL-c5906-681f5b90d15596a17370870c07b7c31f19fa06ad4d619b5ed347ae96c23ee35c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2897%2900150-6$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(97)00150-6$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9094420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Berendji, Denise</creatorcontrib><creatorcontrib>Kolb-Bachofen, Victoria</creatorcontrib><creatorcontrib>Meyer, Klaus L.</creatorcontrib><creatorcontrib>Grapenthin, Olaf</creatorcontrib><creatorcontrib>Weber, Horst</creatorcontrib><creatorcontrib>Wahn, Volker</creatorcontrib><creatorcontrib>Kröncke, Klaus-Dietrich</creatorcontrib><title>Nitric oxide mediates intracytoplasmic and intranuclear zinc release</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We previously described that NO
· leads to destruction of ZnS clusters and release of Zn
2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO
· leads to an increase of cytoplasmic and nuclear free Zn
2+. L929 cells, mouse splenocytes, or rat aorta endothelial cells were labeled with Zinquin-E, a Zn
2+-specific fluorophore, and were treated with two different spontaneous NO donors, S-nitroso-cysteine or DETA/NO. Both NO donors strongly increased the Zn
2+-dependent fluorescence in the cellular cytosol and also in nuclei as compared to controls. NO-dependent Zn
2+ release in splenocytes was quantitated by flow cytometry. These results show for the first time, that nitrosative stress mediates intracellular and intranuclear Zn
2+ release which may be relevant in altering gene expression patterns.
© 1997 Federation of European Biochemical Societies.</description><subject>Animals</subject><subject>Aorta - cytology</subject><subject>Cell Line</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells, Cultured</subject><subject>Cysteine - analogs & derivatives</subject><subject>Cysteine - pharmacology</subject><subject>Cytoplasm - metabolism</subject><subject>Endothelium, Vascular - cytology</subject><subject>Flow cytometry</subject><subject>Fluorescence microscopy</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Mice</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Quinolones - chemistry</subject><subject>Rats</subject><subject>S-Nitrosothiols</subject><subject>Spleen - cytology</subject><subject>Tosyl Compounds - chemistry</subject><subject>Triazenes - pharmacology</subject><subject>Zinc</subject><subject>Zinc - metabolism</subject><subject>Zinquin</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9PAyEQxYnR1Fr9CE32ZPSwCmWB5WS0ttak0YN6Jiw7m2D2T4VdtX56abfpVU8w8968IT8QGhN8RTDh1y8YkyRmQtILKS5DwXDMD9CQpILGNOHpIRruLcfoxPt3HOqUyAEaSCyTZIKH6P7Jts6aqPm2OUQV5Fa34CNbt06bddusSu2roOs675t1Z0rQLvqxtYkchLuHU3RU6NLD2e4cobf57HW6iJfPD4_T22VsmMQ85ikpWCZxThiTXBNBBU4FNlhkwlBSEFlozHWe5JzIjEFOE6FBcjOhAJQZOkLnfe7KNR8d-FZV1hsoS11D03klUkkppjQYWW80rvHeQaFWzlbarRXBakNPbempDRolhdrSUzzMjXcLuiyw2E_tcAV90etftoT1_0LVfHY32SobQYpte7Pqpo-CAOzTglPeWKhN-AEHplV5Y_947C9caJJ_</recordid><startdate>19970317</startdate><enddate>19970317</enddate><creator>Berendji, Denise</creator><creator>Kolb-Bachofen, Victoria</creator><creator>Meyer, Klaus L.</creator><creator>Grapenthin, Olaf</creator><creator>Weber, Horst</creator><creator>Wahn, Volker</creator><creator>Kröncke, Klaus-Dietrich</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970317</creationdate><title>Nitric oxide mediates intracytoplasmic and intranuclear zinc release</title><author>Berendji, Denise ; Kolb-Bachofen, Victoria ; Meyer, Klaus L. ; Grapenthin, Olaf ; Weber, Horst ; Wahn, Volker ; Kröncke, Klaus-Dietrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5906-681f5b90d15596a17370870c07b7c31f19fa06ad4d619b5ed347ae96c23ee35c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Aorta - cytology</topic><topic>Cell Line</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells, Cultured</topic><topic>Cysteine - analogs & derivatives</topic><topic>Cysteine - pharmacology</topic><topic>Cytoplasm - metabolism</topic><topic>Endothelium, Vascular - cytology</topic><topic>Flow cytometry</topic><topic>Fluorescence microscopy</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Mice</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Quinolones - chemistry</topic><topic>Rats</topic><topic>S-Nitrosothiols</topic><topic>Spleen - cytology</topic><topic>Tosyl Compounds - chemistry</topic><topic>Triazenes - pharmacology</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><topic>Zinquin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Berendji, Denise</creatorcontrib><creatorcontrib>Kolb-Bachofen, Victoria</creatorcontrib><creatorcontrib>Meyer, Klaus L.</creatorcontrib><creatorcontrib>Grapenthin, Olaf</creatorcontrib><creatorcontrib>Weber, Horst</creatorcontrib><creatorcontrib>Wahn, Volker</creatorcontrib><creatorcontrib>Kröncke, Klaus-Dietrich</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Berendji, Denise</au><au>Kolb-Bachofen, Victoria</au><au>Meyer, Klaus L.</au><au>Grapenthin, Olaf</au><au>Weber, Horst</au><au>Wahn, Volker</au><au>Kröncke, Klaus-Dietrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitric oxide mediates intracytoplasmic and intranuclear zinc release</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-03-17</date><risdate>1997</risdate><volume>405</volume><issue>1</issue><spage>37</spage><epage>41</epage><pages>37-41</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We previously described that NO
· leads to destruction of ZnS clusters and release of Zn
2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO
· leads to an increase of cytoplasmic and nuclear free Zn
2+. L929 cells, mouse splenocytes, or rat aorta endothelial cells were labeled with Zinquin-E, a Zn
2+-specific fluorophore, and were treated with two different spontaneous NO donors, S-nitroso-cysteine or DETA/NO. Both NO donors strongly increased the Zn
2+-dependent fluorescence in the cellular cytosol and also in nuclei as compared to controls. NO-dependent Zn
2+ release in splenocytes was quantitated by flow cytometry. These results show for the first time, that nitrosative stress mediates intracellular and intranuclear Zn
2+ release which may be relevant in altering gene expression patterns.
© 1997 Federation of European Biochemical Societies.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9094420</pmid><doi>10.1016/S0014-5793(97)00150-6</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1997-03, Vol.405 (1), p.37-41 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Free Content; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Aorta - cytology Cell Line Cell Nucleus - metabolism Cells, Cultured Cysteine - analogs & derivatives Cysteine - pharmacology Cytoplasm - metabolism Endothelium, Vascular - cytology Flow cytometry Fluorescence microscopy Fluorescent Dyes - chemistry Mice Nitric oxide Nitric Oxide - metabolism Quinolones - chemistry Rats S-Nitrosothiols Spleen - cytology Tosyl Compounds - chemistry Triazenes - pharmacology Zinc Zinc - metabolism Zinquin |
| title | Nitric oxide mediates intracytoplasmic and intranuclear zinc release |
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