Vaccination against ovine cysticercosis using a defined recombinant antigen
Cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. Taenia ovis in sheep is a particularly important example. Immunity to reinfection with the larvae has a central role in regulating natural transmission...
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| Veröffentlicht in: | Nature (London) 1989-04, Vol.338 (6216), p.585-587 |
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| creator | JOHNSON, K. S HARRISON, G. B. L LIGHTOWLERS, M. W O'HOY, K. L COUGLE, W. G DEMPSTER, R. P LAWRENCE, S. B VINTON, J. G HEATH, D. D RICKARD, M. D |
| description | Cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. Taenia ovis in sheep is a particularly important example. Immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. As it is impractical to obtain enough oncospheres for a commercial vaccine against these tapeworms, an alternative approach is to use recombinant DNA methods to generate a cheap and plentiful supply of antigens. We report here the expression in Escherichia coli of complementary DNA encoding T. ovis antigens as fusion proteins with the Schistosoma japonicum glutathione S-transferase. Vaccination of sheep with these fusion proteins gave significant, although not complete, immunity against challenge infection with T. ovis eggs. Commercial development of a vaccine is being pursued. |
| doi_str_mv | 10.1038/338585a0 |
| format | Article |
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S ; HARRISON, G. B. L ; LIGHTOWLERS, M. W ; O'HOY, K. L ; COUGLE, W. G ; DEMPSTER, R. P ; LAWRENCE, S. B ; VINTON, J. G ; HEATH, D. D ; RICKARD, M. D</creator><creatorcontrib>JOHNSON, K. S ; HARRISON, G. B. L ; LIGHTOWLERS, M. W ; O'HOY, K. L ; COUGLE, W. G ; DEMPSTER, R. P ; LAWRENCE, S. B ; VINTON, J. G ; HEATH, D. D ; RICKARD, M. D</creatorcontrib><description>Cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. Taenia ovis in sheep is a particularly important example. Immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. As it is impractical to obtain enough oncospheres for a commercial vaccine against these tapeworms, an alternative approach is to use recombinant DNA methods to generate a cheap and plentiful supply of antigens. We report here the expression in Escherichia coli of complementary DNA encoding T. ovis antigens as fusion proteins with the Schistosoma japonicum glutathione S-transferase. Vaccination of sheep with these fusion proteins gave significant, although not complete, immunity against challenge infection with T. ovis eggs. Commercial development of a vaccine is being pursued.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/338585a0</identifier><identifier>PMID: 2648160</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Amino Acid Sequence ; Animals ; Antigens, Helminth ; Applied microbiology ; Base Sequence ; Biological and medical sciences ; cysticercosis ; Cysticercosis - prevention & control ; Cysticercosis - veterinary ; Deoxyribonucleic acid ; DNA ; E coli ; Eggs ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Glutathione Transferase - genetics ; Larvae ; Microbiology ; Molecular Sequence Data ; Ovis ; Parasites ; pigs ; Proteins ; Public health ; Recombinant Fusion Proteins - immunology ; Recombinant Proteins - immunology ; Schistosoma japonicum ; Schistosoma japonicum - enzymology ; Schistosoma japonicum - genetics ; Sheep ; Sheep Diseases - prevention & control ; Taenia - immunology ; Vaccination ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>Nature (London), 1989-04, Vol.338 (6216), p.585-587</ispartof><rights>1990 INIST-CNRS</rights><rights>Copyright Macmillan Journals Ltd. Apr 13, 1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-42100d97fd51eaa2348bc2f95f17c383b9bbc14191b73c1a46fbd7462e39c64e3</citedby><cites>FETCH-LOGICAL-c493t-42100d97fd51eaa2348bc2f95f17c383b9bbc14191b73c1a46fbd7462e39c64e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6924167$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2648160$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JOHNSON, K. S</creatorcontrib><creatorcontrib>HARRISON, G. B. L</creatorcontrib><creatorcontrib>LIGHTOWLERS, M. W</creatorcontrib><creatorcontrib>O'HOY, K. L</creatorcontrib><creatorcontrib>COUGLE, W. G</creatorcontrib><creatorcontrib>DEMPSTER, R. P</creatorcontrib><creatorcontrib>LAWRENCE, S. B</creatorcontrib><creatorcontrib>VINTON, J. G</creatorcontrib><creatorcontrib>HEATH, D. D</creatorcontrib><creatorcontrib>RICKARD, M. D</creatorcontrib><title>Vaccination against ovine cysticercosis using a defined recombinant antigen</title><title>Nature (London)</title><addtitle>Nature</addtitle><description>Cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. Taenia ovis in sheep is a particularly important example. Immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. As it is impractical to obtain enough oncospheres for a commercial vaccine against these tapeworms, an alternative approach is to use recombinant DNA methods to generate a cheap and plentiful supply of antigens. We report here the expression in Escherichia coli of complementary DNA encoding T. ovis antigens as fusion proteins with the Schistosoma japonicum glutathione S-transferase. Vaccination of sheep with these fusion proteins gave significant, although not complete, immunity against challenge infection with T. ovis eggs. Commercial development of a vaccine is being pursued.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigens, Helminth</subject><subject>Applied microbiology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cysticercosis</subject><subject>Cysticercosis - prevention & control</subject><subject>Cysticercosis - veterinary</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Eggs</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione Transferase - genetics</subject><subject>Larvae</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Ovis</subject><subject>Parasites</subject><subject>pigs</subject><subject>Proteins</subject><subject>Public health</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Schistosoma japonicum</subject><subject>Schistosoma japonicum - enzymology</subject><subject>Schistosoma japonicum - genetics</subject><subject>Sheep</subject><subject>Sheep Diseases - prevention & control</subject><subject>Taenia - immunology</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctKxDAUBuAgyjiOgi8gFBFxU81J0lyWMnjDATfqtqRpOmRoU01aYd7e6tRZuJnFIYv_y1mcH6FTwNeAqbyhVGYy03gPTYEJnjIuxT6aYkxkiiXlh-goxhXGOAPBJmhCOJPA8RQ9v2tjnNeda32il9r52CXtl_M2MevYOWODaaOLSR-dXyY6KW01hGUSrGmbYvjpu2QYt7T-GB1Uuo72ZHxn6O3-7nX-mC5eHp7mt4vUMEW7lBHAuFSiKjOwWhPKZGFIpbIKhKGSFqooDDBQUAhqQDNeFaVgnFiqDGeWztDlZu9HaD97G7u8cdHYutbetn3MhVQUJCY7IeUUKyHlTkgABJdkN4QMOBG_8PwfXLV98MNZcoIZA-A8G9DVBpnQxhhslX8E1-iwzgHnP73mf70O9Gzc1xeNLbdwLHLIL8ZcR6PrKmhvXNwyrggDLug3gw2n-Q</recordid><startdate>19890413</startdate><enddate>19890413</enddate><creator>JOHNSON, K. 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S ; HARRISON, G. B. L ; LIGHTOWLERS, M. W ; O'HOY, K. L ; COUGLE, W. G ; DEMPSTER, R. P ; LAWRENCE, S. B ; VINTON, J. G ; HEATH, D. D ; RICKARD, M. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-42100d97fd51eaa2348bc2f95f17c383b9bbc14191b73c1a46fbd7462e39c64e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigens, Helminth</topic><topic>Applied microbiology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cysticercosis</topic><topic>Cysticercosis - prevention & control</topic><topic>Cysticercosis - veterinary</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Eggs</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione Transferase - genetics</topic><topic>Larvae</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Ovis</topic><topic>Parasites</topic><topic>pigs</topic><topic>Proteins</topic><topic>Public health</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Schistosoma japonicum</topic><topic>Schistosoma japonicum - enzymology</topic><topic>Schistosoma japonicum - genetics</topic><topic>Sheep</topic><topic>Sheep Diseases - prevention & control</topic><topic>Taenia - immunology</topic><topic>Vaccination</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JOHNSON, K. S</creatorcontrib><creatorcontrib>HARRISON, G. B. L</creatorcontrib><creatorcontrib>LIGHTOWLERS, M. 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S</au><au>HARRISON, G. B. L</au><au>LIGHTOWLERS, M. W</au><au>O'HOY, K. L</au><au>COUGLE, W. G</au><au>DEMPSTER, R. P</au><au>LAWRENCE, S. B</au><au>VINTON, J. G</au><au>HEATH, D. D</au><au>RICKARD, M. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vaccination against ovine cysticercosis using a defined recombinant antigen</atitle><jtitle>Nature (London)</jtitle><addtitle>Nature</addtitle><date>1989-04-13</date><risdate>1989</risdate><volume>338</volume><issue>6216</issue><spage>585</spage><epage>587</epage><pages>585-587</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. Taenia ovis in sheep is a particularly important example. Immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. As it is impractical to obtain enough oncospheres for a commercial vaccine against these tapeworms, an alternative approach is to use recombinant DNA methods to generate a cheap and plentiful supply of antigens. We report here the expression in Escherichia coli of complementary DNA encoding T. ovis antigens as fusion proteins with the Schistosoma japonicum glutathione S-transferase. Vaccination of sheep with these fusion proteins gave significant, although not complete, immunity against challenge infection with T. ovis eggs. Commercial development of a vaccine is being pursued.</abstract><cop>London</cop><pub>Nature Publishing</pub><pmid>2648160</pmid><doi>10.1038/338585a0</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1989-04, Vol.338 (6216), p.585-587 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Springer Nature - Connect here FIRST to enable access |
| subjects | Amino Acid Sequence Animals Antigens, Helminth Applied microbiology Base Sequence Biological and medical sciences cysticercosis Cysticercosis - prevention & control Cysticercosis - veterinary Deoxyribonucleic acid DNA E coli Eggs Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Glutathione Transferase - genetics Larvae Microbiology Molecular Sequence Data Ovis Parasites pigs Proteins Public health Recombinant Fusion Proteins - immunology Recombinant Proteins - immunology Schistosoma japonicum Schistosoma japonicum - enzymology Schistosoma japonicum - genetics Sheep Sheep Diseases - prevention & control Taenia - immunology Vaccination Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Vaccination against ovine cysticercosis using a defined recombinant antigen |
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