Astrocytes and microglia express inducible nitric oxide synthase in mice with experimental allergic encephalomyelitis
Nitric oxide (NO), produced by inducible NO synthase (iNOS), may play a role in inflammatory demyelinating diseases of the central nervous system (CNS). We show upregulation of iNOS mRNA in CNS of SJL/J mice with experimental allergic encephalomyelitis (EAE). Using antibodies against mouse iNOS, GFA...
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| Veröffentlicht in: | Journal of neuroimmunology 1997-04, Vol.74 (1), p.121-129 |
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| container_title | Journal of neuroimmunology |
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| creator | Tran, Elise H. Hardin-Pouzet, Hélène Verge, Gail Owens, Trevor |
| description | Nitric oxide (NO), produced by inducible NO synthase (iNOS), may play a role in inflammatory demyelinating diseases of the central nervous system (CNS). We show upregulation of iNOS mRNA in CNS of SJL/J mice with experimental allergic encephalomyelitis (EAE). Using antibodies against mouse iNOS, GFAP (a marker for astrocytes) and Mac-1/CD11b (a marker for macrophages/microglia), both astrocytes and macrophages/microglia were identified as iNOS-expressing cells in situ in EAE lesions. GFAP+ astrocytes not associated with inflammatory infiltrates were also found to express iNOS. Because microglia rather than astrocytes are implicated in demyelinating pathology, we propose that microglial NO may be cytopathic whereas astrocyte-derived NO may be protective in EAE. |
| doi_str_mv | 10.1016/S0165-5728(96)00215-9 |
| format | Article |
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We show upregulation of iNOS mRNA in CNS of SJL/J mice with experimental allergic encephalomyelitis (EAE). Using antibodies against mouse iNOS, GFAP (a marker for astrocytes) and Mac-1/CD11b (a marker for macrophages/microglia), both astrocytes and macrophages/microglia were identified as iNOS-expressing cells in situ in EAE lesions. GFAP+ astrocytes not associated with inflammatory infiltrates were also found to express iNOS. Because microglia rather than astrocytes are implicated in demyelinating pathology, we propose that microglial NO may be cytopathic whereas astrocyte-derived NO may be protective in EAE.</description><subject>Animals</subject><subject>Astrocyte</subject><subject>Astrocytes - enzymology</subject><subject>Astrocytes - metabolism</subject><subject>Central Nervous System - cytology</subject><subject>Central Nervous System - enzymology</subject><subject>Central Nervous System - physiopathology</subject><subject>Encephalomyelitis, Autoimmune, Experimental - enzymology</subject><subject>Encephalomyelitis, Autoimmune, Experimental - genetics</subject><subject>Enzyme Induction</subject><subject>Experimental allergic encephalomyelitis</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Microglia</subject><subject>Microglia - enzymology</subject><subject>Multiple sclerosis</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase - genetics</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Tissue Distribution</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1OwzAQhC0EKqXwCEg-ITgE7MRx7BOqKv4kJA7A2XKcDTVykmI7QN-epK249rI-zHy78gxC55RcU0L5zesw8iQvUnEp-RUhKc0TeYCmVBRpIlhKD9H033KMTkL4JITmGZMTNJGUSsnZFPXzEH1n1hEC1m2FG2t89-GsxvC78hACtm3VG1s6wK2N3hrc_doKcFi3cakDDPoIAf6xcTlC4G0DbdQOa-fAfwwEtAZWS-26Zg3ORhtO0VGtXYCz3TtD7_d3b4vH5Pnl4Wkxf04MY1lMeJrWQuSsLgjTJksFmDLTvBZcE81LQlgpa0kzUpSCVzUAl5IW2pCCZCVQms3QxXbvyndfPYSoGhsMOKdb6PqgCiEzwgTfa6S5kJQwORjzrXGIKQQPtVoN_9V-rShRYy9q04saQ1eSq00vauTOdwf6soHqn9oVMei3Wx2GOL4teBWMHXOrrAcTVdXZPRf-AIcxn6M</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>Tran, Elise H.</creator><creator>Hardin-Pouzet, Hélène</creator><creator>Verge, Gail</creator><creator>Owens, Trevor</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Astrocytes and microglia express inducible nitric oxide synthase in mice with experimental allergic encephalomyelitis</title><author>Tran, Elise H. ; Hardin-Pouzet, Hélène ; Verge, Gail ; Owens, Trevor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-622f8854f704ac328ecb3a6f86a0a6b004b9f91307b86dfee69917ac0703be113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Astrocyte</topic><topic>Astrocytes - enzymology</topic><topic>Astrocytes - metabolism</topic><topic>Central Nervous System - cytology</topic><topic>Central Nervous System - enzymology</topic><topic>Central Nervous System - physiopathology</topic><topic>Encephalomyelitis, Autoimmune, Experimental - enzymology</topic><topic>Encephalomyelitis, Autoimmune, Experimental - genetics</topic><topic>Enzyme Induction</topic><topic>Experimental allergic encephalomyelitis</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Microglia</topic><topic>Microglia - enzymology</topic><topic>Multiple sclerosis</topic><topic>Nitric oxide</topic><topic>Nitric Oxide Synthase - genetics</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tran, Elise H.</creatorcontrib><creatorcontrib>Hardin-Pouzet, Hélène</creatorcontrib><creatorcontrib>Verge, Gail</creatorcontrib><creatorcontrib>Owens, Trevor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tran, Elise H.</au><au>Hardin-Pouzet, Hélène</au><au>Verge, Gail</au><au>Owens, Trevor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Astrocytes and microglia express inducible nitric oxide synthase in mice with experimental allergic encephalomyelitis</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>74</volume><issue>1</issue><spage>121</spage><epage>129</epage><pages>121-129</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>Nitric oxide (NO), produced by inducible NO synthase (iNOS), may play a role in inflammatory demyelinating diseases of the central nervous system (CNS). We show upregulation of iNOS mRNA in CNS of SJL/J mice with experimental allergic encephalomyelitis (EAE). Using antibodies against mouse iNOS, GFAP (a marker for astrocytes) and Mac-1/CD11b (a marker for macrophages/microglia), both astrocytes and macrophages/microglia were identified as iNOS-expressing cells in situ in EAE lesions. GFAP+ astrocytes not associated with inflammatory infiltrates were also found to express iNOS. Because microglia rather than astrocytes are implicated in demyelinating pathology, we propose that microglial NO may be cytopathic whereas astrocyte-derived NO may be protective in EAE.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9119964</pmid><doi>10.1016/S0165-5728(96)00215-9</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of neuroimmunology, 1997-04, Vol.74 (1), p.121-129 |
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| subjects | Animals Astrocyte Astrocytes - enzymology Astrocytes - metabolism Central Nervous System - cytology Central Nervous System - enzymology Central Nervous System - physiopathology Encephalomyelitis, Autoimmune, Experimental - enzymology Encephalomyelitis, Autoimmune, Experimental - genetics Enzyme Induction Experimental allergic encephalomyelitis Female Gene Expression Glial Fibrillary Acidic Protein - metabolism Mice Mice, Inbred Strains Microglia Microglia - enzymology Multiple sclerosis Nitric oxide Nitric Oxide Synthase - genetics Nitric Oxide Synthase - metabolism Tissue Distribution |
| title | Astrocytes and microglia express inducible nitric oxide synthase in mice with experimental allergic encephalomyelitis |
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