Optimizing the solid-phase immunofiltration assay: A rapid alternative to immunoassays

The technical variables of the solid-phase immunofiltration assay (SPIA) for the detection of antibodies bound to antigens on a solid-phase filter have been investigated. The binding to solid-phase filters of 125I-labelled axial filament proteins derived from Treponema Phagedenis and the optimal con...

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Veröffentlicht in:	Journal of immunological methods 1989-04, Vol.119 (1), p.35-43
Hauptverfasser:	Ijsselmuiden, O.E., Herbrink, P., Meddens, M.J.M., Tank, B., Stolz, E., Van Eijk, R.V.W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Bacterial - analysis Antigens, Bacterial - analysis Binding Sites, Antibody Binding, Competitive Biological and medical sciences Collodion Filtration - instrumentation Filtration - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Immunoassay - instrumentation Immunoassay - methods Immunofiltration Immunoglobulins Molecular immunology Protein A Rabbits Rapid immunoassay Staphylococcal Protein A Techniques Treponema - analysis Treponema - immunology Tween 20
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