Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics
We recently demonstrated bronchial mucosal expression of IL-4 and IL-5 at the mRNA and protein level in both atopic and nonatopic (intrinsic) asthma. In this report, using double immunohistochemistry (IHC) and in situ hybridization (ISH), we show that 70% of IL-4 and IL-5 mRNA+ signals co-localized...
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| Veröffentlicht in: | The Journal of immunology (1950) 1997-04, Vol.158 (7), p.3539-3544 |
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| container_title | The Journal of immunology (1950) |
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| creator | Ying, S Humbert, M Barkans, J Corrigan, CJ Pfister, R Menz, G Larche, M Robinson, DS Durham, SR Kay, AB |
| description | We recently demonstrated bronchial mucosal expression of IL-4 and IL-5 at the mRNA and protein level in both atopic and nonatopic (intrinsic) asthma. In this report, using double immunohistochemistry (IHC) and in situ hybridization (ISH), we show that 70% of IL-4 and IL-5 mRNA+ signals co-localized to CD3+ T cells, the majority (>70%) of which were CD4+, although CD8+ cells also expressed IL-4 and IL-5 mRNA. The remaining IL-4 and IL-5 mRNA signals co-localized to mast cells and eosinophils. The cellular distribution of these mRNA species did not differ between atopic and nonatopic asthmatics. In contrast, double IHC showed that IL-4 and IL-5 immunoreactivity was predominantly associated with eosinophils and mast cells, with few IL-5 or IL-4 immunoreactive CD3+ T cells detectable. However, total IL-4- or IL-5-positive cells detected by IHC were |
| doi_str_mv | 10.4049/jimmunol.158.7.3539 |
| format | Article |
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In this report, using double immunohistochemistry (IHC) and in situ hybridization (ISH), we show that 70% of IL-4 and IL-5 mRNA+ signals co-localized to CD3+ T cells, the majority (>70%) of which were CD4+, although CD8+ cells also expressed IL-4 and IL-5 mRNA. The remaining IL-4 and IL-5 mRNA signals co-localized to mast cells and eosinophils. The cellular distribution of these mRNA species did not differ between atopic and nonatopic asthmatics. In contrast, double IHC showed that IL-4 and IL-5 immunoreactivity was predominantly associated with eosinophils and mast cells, with few IL-5 or IL-4 immunoreactive CD3+ T cells detectable. However, total IL-4- or IL-5-positive cells detected by IHC were <40% of the total mRNA+ cells, raising the possibility that insufficient cytokine protein accumulated within T cells to enable detection by IHC. We conclude that: 1) in atopic and nonatopic asthma CD8+ T cells, in addition to CD4+ T cells, mast cells and eosinophils express mRNA for IL-4 and IL-5; 2) whereas IL-4 and IL-5 mRNA expression was associated mainly with T cells, immunoreactivity for the corresponding protein products was detectable predominantly in eosinophils and mast cells; and 3) this discrepancy may be partly attributable to the relative insensitivity of double IHC technique that does not allow detection of cytokine protein in T cells where, unlike eosinophils and mast cells, there is no facility for storage and concentration in granules.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.158.7.3539</identifier><identifier>PMID: 9120316</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Adult ; AIDS/HIV ; Asthma - immunology ; Asthma - metabolism ; CD4-Positive T-Lymphocytes - metabolism ; CD8-Positive T-Lymphocytes - metabolism ; Eosinophils - metabolism ; Female ; Humans ; Hypersensitivity, Immediate - immunology ; Hypersensitivity, Immediate - metabolism ; Interleukin-4 - biosynthesis ; Interleukin-4 - genetics ; Interleukin-5 - biosynthesis ; Interleukin-5 - genetics ; Male ; Mast Cells - metabolism ; Middle Aged ; RNA, Messenger - biosynthesis</subject><ispartof>The Journal of immunology (1950), 1997-04, Vol.158 (7), p.3539-3544</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-d81cc308a2847a17c11cf413311d2d6f85e774d7006eeb2eacf45f69c3fdd5e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9120316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ying, S</creatorcontrib><creatorcontrib>Humbert, M</creatorcontrib><creatorcontrib>Barkans, J</creatorcontrib><creatorcontrib>Corrigan, CJ</creatorcontrib><creatorcontrib>Pfister, R</creatorcontrib><creatorcontrib>Menz, G</creatorcontrib><creatorcontrib>Larche, M</creatorcontrib><creatorcontrib>Robinson, DS</creatorcontrib><creatorcontrib>Durham, SR</creatorcontrib><creatorcontrib>Kay, AB</creatorcontrib><title>Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>We recently demonstrated bronchial mucosal expression of IL-4 and IL-5 at the mRNA and protein level in both atopic and nonatopic (intrinsic) asthma. In this report, using double immunohistochemistry (IHC) and in situ hybridization (ISH), we show that 70% of IL-4 and IL-5 mRNA+ signals co-localized to CD3+ T cells, the majority (>70%) of which were CD4+, although CD8+ cells also expressed IL-4 and IL-5 mRNA. The remaining IL-4 and IL-5 mRNA signals co-localized to mast cells and eosinophils. The cellular distribution of these mRNA species did not differ between atopic and nonatopic asthmatics. In contrast, double IHC showed that IL-4 and IL-5 immunoreactivity was predominantly associated with eosinophils and mast cells, with few IL-5 or IL-4 immunoreactive CD3+ T cells detectable. However, total IL-4- or IL-5-positive cells detected by IHC were <40% of the total mRNA+ cells, raising the possibility that insufficient cytokine protein accumulated within T cells to enable detection by IHC. We conclude that: 1) in atopic and nonatopic asthma CD8+ T cells, in addition to CD4+ T cells, mast cells and eosinophils express mRNA for IL-4 and IL-5; 2) whereas IL-4 and IL-5 mRNA expression was associated mainly with T cells, immunoreactivity for the corresponding protein products was detectable predominantly in eosinophils and mast cells; and 3) this discrepancy may be partly attributable to the relative insensitivity of double IHC technique that does not allow detection of cytokine protein in T cells where, unlike eosinophils and mast cells, there is no facility for storage and concentration in granules.</description><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Asthma - immunology</subject><subject>Asthma - metabolism</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Eosinophils - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Hypersensitivity, Immediate - immunology</subject><subject>Hypersensitivity, Immediate - metabolism</subject><subject>Interleukin-4 - biosynthesis</subject><subject>Interleukin-4 - genetics</subject><subject>Interleukin-5 - biosynthesis</subject><subject>Interleukin-5 - genetics</subject><subject>Male</subject><subject>Mast Cells - metabolism</subject><subject>Middle Aged</subject><subject>RNA, Messenger - biosynthesis</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkdFu1DAQRS0EKsvCFyAkP1GqksWOkzh5rLYFKq1AQvtuObZDXMV28CRa-mt8XZ3dBfE0MzpzrzRzEXpLyaYgRfPpwTo3-zBsaFlv-IaVrHmGVrQsSVZVpHqOVoTkeUZ5xV-iVwAPhJCK5MUFumhoThitVujP3e8xGgAbPA4dvt9lBZZeL02J3Y9vN8dpjGEy1i9Vz2rC7SPe3hbXR7a9ra_xHiszDPARmwDWh7G3y7BgJ2E6QZwM2hi86q0ccGvDCNYADu0krTcadzE4LKcwWnVU-uDP0wfrp2g9WHWFk13v5GQVvEYvOjmAeXOua7T_fLfffs1237_cb292mWKcT5muqVKM1DKvCy4pV5SqrqCMUapzXXV1aTgvNE-_MabNjUy07KpGsU7r0rA1en-yTcf_mg1MwllYDpLehBkEr5uc8fT8NWKnRRUDQDSdGKN1Mj4KSsQSmPgbmEiBCS6WwJLq3dl-bp3R_zTnhBK_PPHe_uwPNhoBTg5D2qbicDj85_QE_SeilQ</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>Ying, S</creator><creator>Humbert, M</creator><creator>Barkans, J</creator><creator>Corrigan, CJ</creator><creator>Pfister, R</creator><creator>Menz, G</creator><creator>Larche, M</creator><creator>Robinson, DS</creator><creator>Durham, SR</creator><creator>Kay, AB</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics</title><author>Ying, S ; Humbert, M ; Barkans, J ; Corrigan, CJ ; Pfister, R ; Menz, G ; Larche, M ; Robinson, DS ; Durham, SR ; Kay, AB</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-d81cc308a2847a17c11cf413311d2d6f85e774d7006eeb2eacf45f69c3fdd5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>AIDS/HIV</topic><topic>Asthma - immunology</topic><topic>Asthma - metabolism</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Eosinophils - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Hypersensitivity, Immediate - immunology</topic><topic>Hypersensitivity, Immediate - metabolism</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Interleukin-4 - genetics</topic><topic>Interleukin-5 - biosynthesis</topic><topic>Interleukin-5 - genetics</topic><topic>Male</topic><topic>Mast Cells - metabolism</topic><topic>Middle Aged</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ying, S</creatorcontrib><creatorcontrib>Humbert, M</creatorcontrib><creatorcontrib>Barkans, J</creatorcontrib><creatorcontrib>Corrigan, CJ</creatorcontrib><creatorcontrib>Pfister, R</creatorcontrib><creatorcontrib>Menz, G</creatorcontrib><creatorcontrib>Larche, M</creatorcontrib><creatorcontrib>Robinson, DS</creatorcontrib><creatorcontrib>Durham, SR</creatorcontrib><creatorcontrib>Kay, AB</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ying, S</au><au>Humbert, M</au><au>Barkans, J</au><au>Corrigan, CJ</au><au>Pfister, R</au><au>Menz, G</au><au>Larche, M</au><au>Robinson, DS</au><au>Durham, SR</au><au>Kay, AB</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>158</volume><issue>7</issue><spage>3539</spage><epage>3544</epage><pages>3539-3544</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>We recently demonstrated bronchial mucosal expression of IL-4 and IL-5 at the mRNA and protein level in both atopic and nonatopic (intrinsic) asthma. In this report, using double immunohistochemistry (IHC) and in situ hybridization (ISH), we show that 70% of IL-4 and IL-5 mRNA+ signals co-localized to CD3+ T cells, the majority (>70%) of which were CD4+, although CD8+ cells also expressed IL-4 and IL-5 mRNA. The remaining IL-4 and IL-5 mRNA signals co-localized to mast cells and eosinophils. The cellular distribution of these mRNA species did not differ between atopic and nonatopic asthmatics. In contrast, double IHC showed that IL-4 and IL-5 immunoreactivity was predominantly associated with eosinophils and mast cells, with few IL-5 or IL-4 immunoreactive CD3+ T cells detectable. However, total IL-4- or IL-5-positive cells detected by IHC were <40% of the total mRNA+ cells, raising the possibility that insufficient cytokine protein accumulated within T cells to enable detection by IHC. We conclude that: 1) in atopic and nonatopic asthma CD8+ T cells, in addition to CD4+ T cells, mast cells and eosinophils express mRNA for IL-4 and IL-5; 2) whereas IL-4 and IL-5 mRNA expression was associated mainly with T cells, immunoreactivity for the corresponding protein products was detectable predominantly in eosinophils and mast cells; and 3) this discrepancy may be partly attributable to the relative insensitivity of double IHC technique that does not allow detection of cytokine protein in T cells where, unlike eosinophils and mast cells, there is no facility for storage and concentration in granules.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>9120316</pmid><doi>10.4049/jimmunol.158.7.3539</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 1997-04, Vol.158 (7), p.3539-3544 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult AIDS/HIV Asthma - immunology Asthma - metabolism CD4-Positive T-Lymphocytes - metabolism CD8-Positive T-Lymphocytes - metabolism Eosinophils - metabolism Female Humans Hypersensitivity, Immediate - immunology Hypersensitivity, Immediate - metabolism Interleukin-4 - biosynthesis Interleukin-4 - genetics Interleukin-5 - biosynthesis Interleukin-5 - genetics Male Mast Cells - metabolism Middle Aged RNA, Messenger - biosynthesis |
| title | Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics |
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