Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA
A cloned repetitive DNA sequence ( rep20) was evaluated as a diagnostic probe specific for Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14–16 days after ingesting P. falciparum-infected blood...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1989-01, Vol.32 (2), p.105-112 |
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| container_end_page | 112 |
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| container_issue | 2 |
| container_start_page | 105 |
| container_title | Molecular and biochemical parasitology |
| container_volume | 32 |
| creator | Delves, Chris J. Gomcan, Michael Ridley, Robert G. Matile, Hugues Lensen, Ton H.W. Ponnudurai, Thivi Scaife, John G. |
| description | A cloned repetitive DNA sequence (
rep20) was evaluated as a diagnostic probe specific for
Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14–16 days after ingesting
P. falciparum-infected blood, gave positive signals when examined for the presence of
P. falciparum sporozoite DNA by hybridisation. This correlated with the number of oocysts found in a sample of the same batch of mosquitoes examined by dissection. No positive signals were obtained with 50
Plasmodium berghei-infected mosquitoes probed with the
rep20 sequence. The results indicate that a probe containing
rep20 may be useful in the rapid and specific incrimination of vectors carrying
P. falciparum sporozoites. The value of repetitive DNA in the diagnosis of malaria is discussed. |
| doi_str_mv | 10.1016/0166-6851(89)90061-3 |
| format | Article |
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rep20) was evaluated as a diagnostic probe specific for
Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14–16 days after ingesting
P. falciparum-infected blood, gave positive signals when examined for the presence of
P. falciparum sporozoite DNA by hybridisation. This correlated with the number of oocysts found in a sample of the same batch of mosquitoes examined by dissection. No positive signals were obtained with 50
Plasmodium berghei-infected mosquitoes probed with the
rep20 sequence. The results indicate that a probe containing
rep20 may be useful in the rapid and specific incrimination of vectors carrying
P. falciparum sporozoites. The value of repetitive DNA in the diagnosis of malaria is discussed.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(89)90061-3</identifier><identifier>PMID: 2648141</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Anopheles - isolation & purification ; Anopheles - parasitology ; Anopheles stephensi ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Culicidae ; Diagnosis ; diagnostic techniques ; DNA ; DNA - analysis ; DNA Probes ; Fundamental and applied biological sciences. Psychology ; Human protozoal diseases ; Infectious diseases ; Malaria ; Medical sciences ; monoclonal antibodies ; Parasitic diseases ; Plasmodium falciparum ; Plasmodium falciparum - genetics ; Protozoa ; Protozoal diseases ; Repetitive DNA ; Repetitive Sequences, Nucleic Acid ; Tropical medicine</subject><ispartof>Molecular and biochemical parasitology, 1989-01, Vol.32 (2), p.105-112</ispartof><rights>1989</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-83e6a774fa2aad87788d4c6cf9d1c717d0719eee2b631c2a3afdfc4dd8fa244f3</citedby><cites>FETCH-LOGICAL-c356t-83e6a774fa2aad87788d4c6cf9d1c717d0719eee2b631c2a3afdfc4dd8fa244f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0166685189900613$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7093019$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2648141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Delves, Chris J.</creatorcontrib><creatorcontrib>Gomcan, Michael</creatorcontrib><creatorcontrib>Ridley, Robert G.</creatorcontrib><creatorcontrib>Matile, Hugues</creatorcontrib><creatorcontrib>Lensen, Ton H.W.</creatorcontrib><creatorcontrib>Ponnudurai, Thivi</creatorcontrib><creatorcontrib>Scaife, John G.</creatorcontrib><title>Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>A cloned repetitive DNA sequence (
rep20) was evaluated as a diagnostic probe specific for
Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14–16 days after ingesting
P. falciparum-infected blood, gave positive signals when examined for the presence of
P. falciparum sporozoite DNA by hybridisation. This correlated with the number of oocysts found in a sample of the same batch of mosquitoes examined by dissection. No positive signals were obtained with 50
Plasmodium berghei-infected mosquitoes probed with the
rep20 sequence. The results indicate that a probe containing
rep20 may be useful in the rapid and specific incrimination of vectors carrying
P. falciparum sporozoites. The value of repetitive DNA in the diagnosis of malaria is discussed.</description><subject>Animals</subject><subject>Anopheles - isolation & purification</subject><subject>Anopheles - parasitology</subject><subject>Anopheles stephensi</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Culicidae</subject><subject>Diagnosis</subject><subject>diagnostic techniques</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA Probes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human protozoal diseases</subject><subject>Infectious diseases</subject><subject>Malaria</subject><subject>Medical sciences</subject><subject>monoclonal antibodies</subject><subject>Parasitic diseases</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - genetics</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Repetitive DNA</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Tropical medicine</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd1rFDEUxYModa3-B4rzIKIPo_nafLwUSlu1UFTQPoe7yU2JzEy2yUzB_96su-yjPoQLub9zuJxDyEtGPzDK1Mf2VK_Mmr0z9r2lVLFePCIrZjTvreTmMVkdkafkWa2_KKVrrdQJOeFKGibZivjrgNOcYvIwpzx1OXbfB6hjDmkZuwiDT1soy9inKaKfMXRjrvdLmjPWbqlpuuug25a8wc7naYY07b4KbnFOc3rA7vLr-XPypBlVfHGYp-T209XPiy_9zbfP1xfnN70XazX3RqACrWUEDhCM1sYE6ZWPNjCvmQ5UM4uIfKME8xwExBC9DME0hZRRnJK3e992z_2CdXZjqh6HASbMS3XaWE41p_8F2ZpJK4RuoNyDvuRaC0a3LWmE8tsx6nYluF3CbpewM9b9LcGJJnt18F82I4aj6JB627857KF6GGKByad6xDS1gjLbsNd7LEJ2cFcacvuDUyYoV8ZazhtxtiewxfqQsLjqE04eQyqtLRdy-velfwCN_630</recordid><startdate>19890101</startdate><enddate>19890101</enddate><creator>Delves, Chris J.</creator><creator>Gomcan, Michael</creator><creator>Ridley, Robert G.</creator><creator>Matile, Hugues</creator><creator>Lensen, Ton H.W.</creator><creator>Ponnudurai, Thivi</creator><creator>Scaife, John G.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19890101</creationdate><title>Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA</title><author>Delves, Chris J. ; Gomcan, Michael ; Ridley, Robert G. ; Matile, Hugues ; Lensen, Ton H.W. ; Ponnudurai, Thivi ; Scaife, John G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-83e6a774fa2aad87788d4c6cf9d1c717d0719eee2b631c2a3afdfc4dd8fa244f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Anopheles - isolation & purification</topic><topic>Anopheles - parasitology</topic><topic>Anopheles stephensi</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Culicidae</topic><topic>Diagnosis</topic><topic>diagnostic techniques</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA Probes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human protozoal diseases</topic><topic>Infectious diseases</topic><topic>Malaria</topic><topic>Medical sciences</topic><topic>monoclonal antibodies</topic><topic>Parasitic diseases</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - genetics</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><topic>Repetitive DNA</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Delves, Chris J.</creatorcontrib><creatorcontrib>Gomcan, Michael</creatorcontrib><creatorcontrib>Ridley, Robert G.</creatorcontrib><creatorcontrib>Matile, Hugues</creatorcontrib><creatorcontrib>Lensen, Ton H.W.</creatorcontrib><creatorcontrib>Ponnudurai, Thivi</creatorcontrib><creatorcontrib>Scaife, John G.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Delves, Chris J.</au><au>Gomcan, Michael</au><au>Ridley, Robert G.</au><au>Matile, Hugues</au><au>Lensen, Ton H.W.</au><au>Ponnudurai, Thivi</au><au>Scaife, John G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1989-01-01</date><risdate>1989</risdate><volume>32</volume><issue>2</issue><spage>105</spage><epage>112</epage><pages>105-112</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>A cloned repetitive DNA sequence (
rep20) was evaluated as a diagnostic probe specific for
Plasmodium falciparum sporozoites using experimentally infected mosquitoes squashed directly on nylon filters. Head/thorax portions of mosquitoes, killed 14–16 days after ingesting
P. falciparum-infected blood, gave positive signals when examined for the presence of
P. falciparum sporozoite DNA by hybridisation. This correlated with the number of oocysts found in a sample of the same batch of mosquitoes examined by dissection. No positive signals were obtained with 50
Plasmodium berghei-infected mosquitoes probed with the
rep20 sequence. The results indicate that a probe containing
rep20 may be useful in the rapid and specific incrimination of vectors carrying
P. falciparum sporozoites. The value of repetitive DNA in the diagnosis of malaria is discussed.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>2648141</pmid><doi>10.1016/0166-6851(89)90061-3</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1989-01, Vol.32 (2), p.105-112 |
| issn | 0166-6851 1872-9428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78920720 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Anopheles - isolation & purification Anopheles - parasitology Anopheles stephensi Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Culicidae Diagnosis diagnostic techniques DNA DNA - analysis DNA Probes Fundamental and applied biological sciences. Psychology Human protozoal diseases Infectious diseases Malaria Medical sciences monoclonal antibodies Parasitic diseases Plasmodium falciparum Plasmodium falciparum - genetics Protozoa Protozoal diseases Repetitive DNA Repetitive Sequences, Nucleic Acid Tropical medicine |
| title | Identification of Plasmodium falciparum-infected mosquitoes using a probe containing repetitive DNA |
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