Protease-activated receptor 3 is a second thrombin receptor in humans
Thrombin is a coagulation protease that activates platelets, leukocytes, endothelial and mesenchymal cells at sites of vascular injury, acting partly through an unusual proteolytically activated G-protein-coupled receptor 1–3 . Knockout of the gene encoding this receptor provided definitive evidence...
Gespeichert in:
| Veröffentlicht in: | Nature (London) 1997-04, Vol.386 (6624), p.502-506 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 506 |
|---|---|
| container_issue | 6624 |
| container_start_page | 502 |
| container_title | Nature (London) |
| container_volume | 386 |
| creator | Ishihara, Hiroaki Connolly, Andrew J. Zeng, Dewan Kahn, Mark L. Wu Zheng, Yao Timmons, Courtney Tram, Tracy Coughlin, Shaun R. |
| description | Thrombin is a coagulation protease that activates platelets, leukocytes, endothelial and mesenchymal cells at sites of vascular injury, acting partly through an unusual proteolytically activated G-protein-coupled receptor
1–3
. Knockout of the gene encoding this receptor provided definitive evidence for a second thrombin receptor in mouse platelets and for tissue-specific roles for different thrombin receptors
4
. We now report the cloning and characterization of a new human thrombin receptor, designated protease-activated receptor 3 (PAR3). PAR3 can mediate throm-bin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues, including human bone marrow and mouse megakaryocytes, making it a candidate for the sought-after second platelet thrombin receptor. PAR3 provides a new tool for understanding thrombin signalling and a possible target for therapeutics designed selectively to block thrombotic, inflammatory and proliferative responses to thrombin. |
| doi_str_mv | 10.1038/386502a0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78912777</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>11404225</sourcerecordid><originalsourceid>FETCH-LOGICAL-c497t-fa3f9fef26ecd20c4e14278089096b40f0edfa74c421060ff131ab960fea37d63</originalsourceid><addsrcrecordid>eNqFkVtLw0AQhRdRtF7APyAEES8P0dlLdjePUuoFCvqgz2G7mdWUJqm7ieC_d0urBQV9mgPn48wMh5BDCpcUuL7iWmbADGyQARVKpkJqtUkGAEynoLncIbshTAEgo0psk-0ctBIUBmT06NsOTcDU2K56Nx2WiUeL8671CU-qkJgkoG2bMulefVtPqmbtR_3a16YJ-2TLmVnAg9XcI883o6fhXTp-uL0fXo9TK3LVpc5wlzt0TKItGViBVDClQeeQy4kAB1g6o4QVjIIE5yinZpJHhYarUvI9crrMnfv2rcfQFXUVLM5mpsG2D4XSOWVKqQie_Q0Krjhjmv0bSTMtKWQQweMf4LTtfRPfLRgIkWWCLg48X0LWtyF4dMXcV7XxHwWFYtFU8dVURI9Wef2kxvIbXFUT_ZOVb4I1M-dNY6vwjTEJGnIdsYslFqLTvKBfn_Vr5Sf-xKVi</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>204455416</pqid></control><display><type>article</type><title>Protease-activated receptor 3 is a second thrombin receptor in humans</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Nature</source><creator>Ishihara, Hiroaki ; Connolly, Andrew J. ; Zeng, Dewan ; Kahn, Mark L. ; Wu Zheng, Yao ; Timmons, Courtney ; Tram, Tracy ; Coughlin, Shaun R.</creator><creatorcontrib>Ishihara, Hiroaki ; Connolly, Andrew J. ; Zeng, Dewan ; Kahn, Mark L. ; Wu Zheng, Yao ; Timmons, Courtney ; Tram, Tracy ; Coughlin, Shaun R.</creatorcontrib><description>Thrombin is a coagulation protease that activates platelets, leukocytes, endothelial and mesenchymal cells at sites of vascular injury, acting partly through an unusual proteolytically activated G-protein-coupled receptor
1–3
. Knockout of the gene encoding this receptor provided definitive evidence for a second thrombin receptor in mouse platelets and for tissue-specific roles for different thrombin receptors
4
. We now report the cloning and characterization of a new human thrombin receptor, designated protease-activated receptor 3 (PAR3). PAR3 can mediate throm-bin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues, including human bone marrow and mouse megakaryocytes, making it a candidate for the sought-after second platelet thrombin receptor. PAR3 provides a new tool for understanding thrombin signalling and a possible target for therapeutics designed selectively to block thrombotic, inflammatory and proliferative responses to thrombin.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/386502a0</identifier><identifier>PMID: 9087410</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Blood coagulation. Blood cells ; Blood Platelets - metabolism ; Bone marrow ; Cloning ; Cloning, Molecular ; COS Cells ; DNA, Complementary ; Endopeptidases - metabolism ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods, hemostasis, fibrinolysis ; Humanities and Social Sciences ; Humans ; Hydrolysis ; letter ; Medical research ; Mice ; Mice, Inbred C57BL ; Molecular and cellular biology ; Molecular Mimicry ; Molecular Sequence Data ; multidisciplinary ; Mutation ; Rats ; Receptors, Thrombin - genetics ; Receptors, Thrombin - metabolism ; RNA, Messenger - metabolism ; Science ; Science (multidisciplinary) ; Sequence Homology, Amino Acid ; Signal Transduction ; Thrombin - metabolism ; Tissue Distribution</subject><ispartof>Nature (London), 1997-04, Vol.386 (6624), p.502-506</ispartof><rights>Springer Nature Limited 1997</rights><rights>1997 INIST-CNRS</rights><rights>Copyright Macmillan Journals Ltd. Apr 3, 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-fa3f9fef26ecd20c4e14278089096b40f0edfa74c421060ff131ab960fea37d63</citedby><cites>FETCH-LOGICAL-c497t-fa3f9fef26ecd20c4e14278089096b40f0edfa74c421060ff131ab960fea37d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/386502a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/386502a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2608098$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9087410$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishihara, Hiroaki</creatorcontrib><creatorcontrib>Connolly, Andrew J.</creatorcontrib><creatorcontrib>Zeng, Dewan</creatorcontrib><creatorcontrib>Kahn, Mark L.</creatorcontrib><creatorcontrib>Wu Zheng, Yao</creatorcontrib><creatorcontrib>Timmons, Courtney</creatorcontrib><creatorcontrib>Tram, Tracy</creatorcontrib><creatorcontrib>Coughlin, Shaun R.</creatorcontrib><title>Protease-activated receptor 3 is a second thrombin receptor in humans</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Thrombin is a coagulation protease that activates platelets, leukocytes, endothelial and mesenchymal cells at sites of vascular injury, acting partly through an unusual proteolytically activated G-protein-coupled receptor
1–3
. Knockout of the gene encoding this receptor provided definitive evidence for a second thrombin receptor in mouse platelets and for tissue-specific roles for different thrombin receptors
4
. We now report the cloning and characterization of a new human thrombin receptor, designated protease-activated receptor 3 (PAR3). PAR3 can mediate throm-bin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues, including human bone marrow and mouse megakaryocytes, making it a candidate for the sought-after second platelet thrombin receptor. PAR3 provides a new tool for understanding thrombin signalling and a possible target for therapeutics designed selectively to block thrombotic, inflammatory and proliferative responses to thrombin.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - metabolism</subject><subject>Bone marrow</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>COS Cells</subject><subject>DNA, Complementary</subject><subject>Endopeptidases - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods, hemostasis, fibrinolysis</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>letter</subject><subject>Medical research</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular and cellular biology</subject><subject>Molecular Mimicry</subject><subject>Molecular Sequence Data</subject><subject>multidisciplinary</subject><subject>Mutation</subject><subject>Rats</subject><subject>Receptors, Thrombin - genetics</subject><subject>Receptors, Thrombin - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction</subject><subject>Thrombin - metabolism</subject><subject>Tissue Distribution</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkVtLw0AQhRdRtF7APyAEES8P0dlLdjePUuoFCvqgz2G7mdWUJqm7ieC_d0urBQV9mgPn48wMh5BDCpcUuL7iWmbADGyQARVKpkJqtUkGAEynoLncIbshTAEgo0psk-0ctBIUBmT06NsOTcDU2K56Nx2WiUeL8671CU-qkJgkoG2bMulefVtPqmbtR_3a16YJ-2TLmVnAg9XcI883o6fhXTp-uL0fXo9TK3LVpc5wlzt0TKItGViBVDClQeeQy4kAB1g6o4QVjIIE5yinZpJHhYarUvI9crrMnfv2rcfQFXUVLM5mpsG2D4XSOWVKqQie_Q0Krjhjmv0bSTMtKWQQweMf4LTtfRPfLRgIkWWCLg48X0LWtyF4dMXcV7XxHwWFYtFU8dVURI9Wef2kxvIbXFUT_ZOVb4I1M-dNY6vwjTEJGnIdsYslFqLTvKBfn_Vr5Sf-xKVi</recordid><startdate>19970403</startdate><enddate>19970403</enddate><creator>Ishihara, Hiroaki</creator><creator>Connolly, Andrew J.</creator><creator>Zeng, Dewan</creator><creator>Kahn, Mark L.</creator><creator>Wu Zheng, Yao</creator><creator>Timmons, Courtney</creator><creator>Tram, Tracy</creator><creator>Coughlin, Shaun R.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7TG</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>S0X</scope><scope>SOI</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope></search><sort><creationdate>19970403</creationdate><title>Protease-activated receptor 3 is a second thrombin receptor in humans</title><author>Ishihara, Hiroaki ; Connolly, Andrew J. ; Zeng, Dewan ; Kahn, Mark L. ; Wu Zheng, Yao ; Timmons, Courtney ; Tram, Tracy ; Coughlin, Shaun R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-fa3f9fef26ecd20c4e14278089096b40f0edfa74c421060ff131ab960fea37d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - metabolism</topic><topic>Bone marrow</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>COS Cells</topic><topic>DNA, Complementary</topic><topic>Endopeptidases - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods, hemostasis, fibrinolysis</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>letter</topic><topic>Medical research</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular and cellular biology</topic><topic>Molecular Mimicry</topic><topic>Molecular Sequence Data</topic><topic>multidisciplinary</topic><topic>Mutation</topic><topic>Rats</topic><topic>Receptors, Thrombin - genetics</topic><topic>Receptors, Thrombin - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction</topic><topic>Thrombin - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishihara, Hiroaki</creatorcontrib><creatorcontrib>Connolly, Andrew J.</creatorcontrib><creatorcontrib>Zeng, Dewan</creatorcontrib><creatorcontrib>Kahn, Mark L.</creatorcontrib><creatorcontrib>Wu Zheng, Yao</creatorcontrib><creatorcontrib>Timmons, Courtney</creatorcontrib><creatorcontrib>Tram, Tracy</creatorcontrib><creatorcontrib>Coughlin, Shaun R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>ProQuest Research Library</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>Environment Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishihara, Hiroaki</au><au>Connolly, Andrew J.</au><au>Zeng, Dewan</au><au>Kahn, Mark L.</au><au>Wu Zheng, Yao</au><au>Timmons, Courtney</au><au>Tram, Tracy</au><au>Coughlin, Shaun R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protease-activated receptor 3 is a second thrombin receptor in humans</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1997-04-03</date><risdate>1997</risdate><volume>386</volume><issue>6624</issue><spage>502</spage><epage>506</epage><pages>502-506</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Thrombin is a coagulation protease that activates platelets, leukocytes, endothelial and mesenchymal cells at sites of vascular injury, acting partly through an unusual proteolytically activated G-protein-coupled receptor
1–3
. Knockout of the gene encoding this receptor provided definitive evidence for a second thrombin receptor in mouse platelets and for tissue-specific roles for different thrombin receptors
4
. We now report the cloning and characterization of a new human thrombin receptor, designated protease-activated receptor 3 (PAR3). PAR3 can mediate throm-bin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues, including human bone marrow and mouse megakaryocytes, making it a candidate for the sought-after second platelet thrombin receptor. PAR3 provides a new tool for understanding thrombin signalling and a possible target for therapeutics designed selectively to block thrombotic, inflammatory and proliferative responses to thrombin.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>9087410</pmid><doi>10.1038/386502a0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1997-04, Vol.386 (6624), p.502-506 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78912777 |
| source | MEDLINE; SpringerLink Journals; Nature |
| subjects | Amino Acid Sequence Animals Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - metabolism Bone marrow Cloning Cloning, Molecular COS Cells DNA, Complementary Endopeptidases - metabolism Fundamental and applied biological sciences. Psychology General aspects, investigation methods, hemostasis, fibrinolysis Humanities and Social Sciences Humans Hydrolysis letter Medical research Mice Mice, Inbred C57BL Molecular and cellular biology Molecular Mimicry Molecular Sequence Data multidisciplinary Mutation Rats Receptors, Thrombin - genetics Receptors, Thrombin - metabolism RNA, Messenger - metabolism Science Science (multidisciplinary) Sequence Homology, Amino Acid Signal Transduction Thrombin - metabolism Tissue Distribution |
| title | Protease-activated receptor 3 is a second thrombin receptor in humans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T04%3A05%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protease-activated%20receptor%203%20is%20a%20second%20thrombin%20receptor%20in%20humans&rft.jtitle=Nature%20(London)&rft.au=Ishihara,%20Hiroaki&rft.date=1997-04-03&rft.volume=386&rft.issue=6624&rft.spage=502&rft.epage=506&rft.pages=502-506&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/386502a0&rft_dat=%3Cproquest_cross%3E11404225%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204455416&rft_id=info:pmid/9087410&rfr_iscdi=true |