Localization of PDGF α-receptor in the developing and mature human kidney
Localization of PDGF α-receptor in the developing and mature human kidney. Using in situ hybridization and immunocytochemistry we describe the renal localization of the PDGF α-receptor. PDGF α-receptor mRNA was uniformly present in human metanephric kidney in interstitial cells and vascular arcades...
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| Veröffentlicht in: | Kidney international 1997-04, Vol.51 (4), p.1140-1150 |
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| container_title | Kidney international |
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| creator | Floege, Jürgen Hudkins, Kelly L. Seifert, Ronald A. Francki, Aleksandar Bowen-Pope, Daniel F. Alpers, Charles E. |
| description | Localization of PDGF α-receptor in the developing and mature human kidney. Using in situ hybridization and immunocytochemistry we describe the renal localization of the PDGF α-receptor. PDGF α-receptor mRNA was uniformly present in human metanephric kidney in interstitial cells and vascular arcades that course through the blastema. PDGF α-receptor mRNA was present in some mesangial structures in early glomeruli, but was largely lost as glomeruli matured. It was present in adventitial fibroblasts, but usually not in vascular smooth muscle cells or endothelial cells of the fetal vasculature. This pattern persisted in adult kidneys, with extensive expression of mRNA by interstitial cells and only occasional expression by mesangial cells. All in situ hybridization findings were corroborated by immunocytochemistry. Double immunolabeling confirmed the rare expression of the PDGF α-receptor protein by vascular smooth muscle cells and the absence of its expression by endothelial cells. Given that both PDGF A- and B-chain can promote smooth muscle cell and fibroblast migration and proliferation and that both signal through the PDGF α-receptor, these data suggest that PDGF α-receptor may play important roles in the early vasculogenesis of the fetal kidney as well as in the pathogenesis of renal interstitial fibrosis. |
| doi_str_mv | 10.1038/ki.1997.157 |
| format | Article |
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Using in situ hybridization and immunocytochemistry we describe the renal localization of the PDGF α-receptor. PDGF α-receptor mRNA was uniformly present in human metanephric kidney in interstitial cells and vascular arcades that course through the blastema. PDGF α-receptor mRNA was present in some mesangial structures in early glomeruli, but was largely lost as glomeruli matured. It was present in adventitial fibroblasts, but usually not in vascular smooth muscle cells or endothelial cells of the fetal vasculature. This pattern persisted in adult kidneys, with extensive expression of mRNA by interstitial cells and only occasional expression by mesangial cells. All in situ hybridization findings were corroborated by immunocytochemistry. Double immunolabeling confirmed the rare expression of the PDGF α-receptor protein by vascular smooth muscle cells and the absence of its expression by endothelial cells. Given that both PDGF A- and B-chain can promote smooth muscle cell and fibroblast migration and proliferation and that both signal through the PDGF α-receptor, these data suggest that PDGF α-receptor may play important roles in the early vasculogenesis of the fetal kidney as well as in the pathogenesis of renal interstitial fibrosis.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1038/ki.1997.157</identifier><identifier>PMID: 9083280</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Endothelium, Vascular - cytology ; Endothelium, Vascular - embryology ; Endothelium, Vascular - metabolism ; Fetus - cytology ; Fetus - metabolism ; Fundamental and applied biological sciences. Psychology ; Glomerular Mesangium - cytology ; Glomerular Mesangium - embryology ; Glomerular Mesangium - metabolism ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Kidney - cytology ; Kidney - embryology ; Kidney - metabolism ; Kidney Diseases - etiology ; Kidney Diseases - metabolism ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - embryology ; Muscle, Smooth, Vascular - metabolism ; Receptor, Platelet-Derived Growth Factor alpha ; Receptors, Platelet-Derived Growth Factor - genetics ; Receptors, Platelet-Derived Growth Factor - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Vertebrates: urinary system</subject><ispartof>Kidney international, 1997-04, Vol.51 (4), p.1140-1150</ispartof><rights>1997 International Society of Nephrology</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-41d5ad77b041b32060ff21085865cba5d626e143cb45ce50223f64e7fcd00b7f3</citedby><cites>FETCH-LOGICAL-c420t-41d5ad77b041b32060ff21085865cba5d626e143cb45ce50223f64e7fcd00b7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23928,23929,25138,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2641896$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9083280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Floege, Jürgen</creatorcontrib><creatorcontrib>Hudkins, Kelly L.</creatorcontrib><creatorcontrib>Seifert, Ronald A.</creatorcontrib><creatorcontrib>Francki, Aleksandar</creatorcontrib><creatorcontrib>Bowen-Pope, Daniel F.</creatorcontrib><creatorcontrib>Alpers, Charles E.</creatorcontrib><title>Localization of PDGF α-receptor in the developing and mature human kidney</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>Localization of PDGF α-receptor in the developing and mature human kidney. Using in situ hybridization and immunocytochemistry we describe the renal localization of the PDGF α-receptor. PDGF α-receptor mRNA was uniformly present in human metanephric kidney in interstitial cells and vascular arcades that course through the blastema. PDGF α-receptor mRNA was present in some mesangial structures in early glomeruli, but was largely lost as glomeruli matured. It was present in adventitial fibroblasts, but usually not in vascular smooth muscle cells or endothelial cells of the fetal vasculature. This pattern persisted in adult kidneys, with extensive expression of mRNA by interstitial cells and only occasional expression by mesangial cells. All in situ hybridization findings were corroborated by immunocytochemistry. Double immunolabeling confirmed the rare expression of the PDGF α-receptor protein by vascular smooth muscle cells and the absence of its expression by endothelial cells. Given that both PDGF A- and B-chain can promote smooth muscle cell and fibroblast migration and proliferation and that both signal through the PDGF α-receptor, these data suggest that PDGF α-receptor may play important roles in the early vasculogenesis of the fetal kidney as well as in the pathogenesis of renal interstitial fibrosis.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - embryology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fetus - cytology</subject><subject>Fetus - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glomerular Mesangium - cytology</subject><subject>Glomerular Mesangium - embryology</subject><subject>Glomerular Mesangium - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Kidney - cytology</subject><subject>Kidney - embryology</subject><subject>Kidney - metabolism</subject><subject>Kidney Diseases - etiology</subject><subject>Kidney Diseases - metabolism</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - embryology</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Receptor, Platelet-Derived Growth Factor alpha</subject><subject>Receptors, Platelet-Derived Growth Factor - genetics</subject><subject>Receptors, Platelet-Derived Growth Factor - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Vertebrates: urinary system</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1u1DAURi0EKtPCijXCC8QGZfBPnDjLqtBCNRIsYG059jU1k9iDnVQqb9UX6TPVQ0az6urq6jv67tVB6A0la0q4_LT1a9p17ZqK9hlaUcF4RVshnqMVIVJUTHD5Ep3m_IeUvePkBJ10RHImyQpdb6LRg_-nJx8Djg7_-Hx1iR_uqwQGdlNM2Ac83QC2cAtD3PnwG-tg8ainOQG-mUcd8NbbAHev0AunhwyvD_MM_br88vPia7X5fvXt4nxTmZqRqaqpFdq2bU9q2nNGGuIco-VR2QjTa2Eb1gCtuelrYUAQxrhramidsYT0reNn6MPSu0vx7wx5UqPPBoZBB4hzVq3sSNdIWsCPC2hSzDmBU7vkR53uFCVqb05tvdqbU8Vcod8eaud-BHtkD6pK_v6Q61yUuaSD8fmIsaamsmsK9m7Bwn9Dx3zr95eWQ2IhoEi69ZBUNh6CAeuL9EnZ6J988BH0IZH3</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>Floege, Jürgen</creator><creator>Hudkins, Kelly L.</creator><creator>Seifert, Ronald A.</creator><creator>Francki, Aleksandar</creator><creator>Bowen-Pope, Daniel F.</creator><creator>Alpers, Charles E.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Localization of PDGF α-receptor in the developing and mature human kidney</title><author>Floege, Jürgen ; Hudkins, Kelly L. ; Seifert, Ronald A. ; Francki, Aleksandar ; Bowen-Pope, Daniel F. ; Alpers, Charles E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-41d5ad77b041b32060ff21085865cba5d626e143cb45ce50223f64e7fcd00b7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - embryology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fetus - cytology</topic><topic>Fetus - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glomerular Mesangium - cytology</topic><topic>Glomerular Mesangium - embryology</topic><topic>Glomerular Mesangium - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Kidney - cytology</topic><topic>Kidney - embryology</topic><topic>Kidney - metabolism</topic><topic>Kidney Diseases - etiology</topic><topic>Kidney Diseases - metabolism</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - embryology</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Receptor, Platelet-Derived Growth Factor alpha</topic><topic>Receptors, Platelet-Derived Growth Factor - genetics</topic><topic>Receptors, Platelet-Derived Growth Factor - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Floege, Jürgen</creatorcontrib><creatorcontrib>Hudkins, Kelly L.</creatorcontrib><creatorcontrib>Seifert, Ronald A.</creatorcontrib><creatorcontrib>Francki, Aleksandar</creatorcontrib><creatorcontrib>Bowen-Pope, Daniel F.</creatorcontrib><creatorcontrib>Alpers, Charles E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Floege, Jürgen</au><au>Hudkins, Kelly L.</au><au>Seifert, Ronald A.</au><au>Francki, Aleksandar</au><au>Bowen-Pope, Daniel F.</au><au>Alpers, Charles E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of PDGF α-receptor in the developing and mature human kidney</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>51</volume><issue>4</issue><spage>1140</spage><epage>1150</epage><pages>1140-1150</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract>Localization of PDGF α-receptor in the developing and mature human kidney. Using in situ hybridization and immunocytochemistry we describe the renal localization of the PDGF α-receptor. PDGF α-receptor mRNA was uniformly present in human metanephric kidney in interstitial cells and vascular arcades that course through the blastema. PDGF α-receptor mRNA was present in some mesangial structures in early glomeruli, but was largely lost as glomeruli matured. It was present in adventitial fibroblasts, but usually not in vascular smooth muscle cells or endothelial cells of the fetal vasculature. This pattern persisted in adult kidneys, with extensive expression of mRNA by interstitial cells and only occasional expression by mesangial cells. All in situ hybridization findings were corroborated by immunocytochemistry. Double immunolabeling confirmed the rare expression of the PDGF α-receptor protein by vascular smooth muscle cells and the absence of its expression by endothelial cells. Given that both PDGF A- and B-chain can promote smooth muscle cell and fibroblast migration and proliferation and that both signal through the PDGF α-receptor, these data suggest that PDGF α-receptor may play important roles in the early vasculogenesis of the fetal kidney as well as in the pathogenesis of renal interstitial fibrosis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9083280</pmid><doi>10.1038/ki.1997.157</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Biological and medical sciences Endothelium, Vascular - cytology Endothelium, Vascular - embryology Endothelium, Vascular - metabolism Fetus - cytology Fetus - metabolism Fundamental and applied biological sciences. Psychology Glomerular Mesangium - cytology Glomerular Mesangium - embryology Glomerular Mesangium - metabolism Humans Immunohistochemistry In Situ Hybridization Kidney - cytology Kidney - embryology Kidney - metabolism Kidney Diseases - etiology Kidney Diseases - metabolism Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - embryology Muscle, Smooth, Vascular - metabolism Receptor, Platelet-Derived Growth Factor alpha Receptors, Platelet-Derived Growth Factor - genetics Receptors, Platelet-Derived Growth Factor - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Vertebrates: urinary system |
| title | Localization of PDGF α-receptor in the developing and mature human kidney |
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