Transient inositol (1,4,5)trisphosphate accumulation under vasopressin stimulation in WRK1 cells: correlation with intracellular calcium mobilization
In the rat mammary tumoral cell line (WRK1 cells), vasopressin was previously described to stimulate a phospholipase C. In this study, we have analysed the effect of vasopressin both on intracellular calcium mobilization and on the accumulation of inositol phosphates. Maximal concentration of vasopr...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1989-03, Vol.159 (3), p.953-960 |
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| creator | MOUILLAC, B BALESTRE, M.-N GUILLON, G |
| description | In the rat mammary tumoral cell line (WRK1 cells), vasopressin was previously described to stimulate a phospholipase C. In this study, we have analysed the effect of vasopressin both on intracellular calcium mobilization and on the accumulation of inositol phosphates. Maximal concentration of vasopressin simultaneously induces an accumulation of Ins(1,4,5)P3 and a rise of intracellular calcium concentration. Both these two phenomena are transient and exhibit similar kinetics. A sustained accumulation of InsP2, Ins(1,3,4)P3 and InsP are observed later. Yet no stimulation of InsP4 can be objectified. These results indicate that Ins(1,4,5)P3 is the major inositol phosphate involved in intracellular calcium mobilization. |
| doi_str_mv | 10.1016/0006-291X(89)92201-8 |
| format | Article |
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In this study, we have analysed the effect of vasopressin both on intracellular calcium mobilization and on the accumulation of inositol phosphates. Maximal concentration of vasopressin simultaneously induces an accumulation of Ins(1,4,5)P3 and a rise of intracellular calcium concentration. Both these two phenomena are transient and exhibit similar kinetics. A sustained accumulation of InsP2, Ins(1,3,4)P3 and InsP are observed later. Yet no stimulation of InsP4 can be objectified. These results indicate that Ins(1,4,5)P3 is the major inositol phosphate involved in intracellular calcium mobilization.</description><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Arginine Vasopressin - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cell Line</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inositol 1,4,5-Trisphosphate</subject><subject>Inositol Phosphates - isolation & purification</subject><subject>Inositol Phosphates - metabolism</subject><subject>Kinetics</subject><subject>Mammary Neoplasms, Experimental</subject><subject>Proteins</subject><subject>Rats</subject><subject>Sugar Phosphates - metabolism</subject><subject>Type C Phospholipases - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kV-L1TAQxYMo63X1GyjkQWQXtjqTpm3imyz-wwVBVvQtpLkJG2mbayZV9Hv4fW13y30YwvA7ZwjnMPYU4SUCtq8AoK2Exu9nSp9rIQArdY_tEDRUAkHeZ7uj5CF7RPQDAFG2-oSdiE7JVsGO_bvOdqLop8LjlCiWNPAzvJAXzXnJkQ43aRlbPLfOzeM82BLTxOdp7zP_ZSkdsieKE6cSj3RZv335hNz5YaDX3KWc_YZ-x3Kz8JLtChdD5s4OLs4jH1Mfh_j3VveYPQh2IP9ke0_Z13dvry8_VFef33-8fHNVubrGUjWusXul666rIfSATjVyH1TTikZ6J4JDcE2o-87KgLL2IvS9AK1d4wCss_Upe3F395DTz9lTMWOk9Wd28mkm0ykNSgu9COWd0OVElH0whxxHm_8YBLO2YdaozRq1UdrctmHUYnu23Z_70e-Ppi3-hT_fuKUlh7B04SIdZW0LukOs_wN5e5Ym</recordid><startdate>19890331</startdate><enddate>19890331</enddate><creator>MOUILLAC, B</creator><creator>BALESTRE, M.-N</creator><creator>GUILLON, G</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890331</creationdate><title>Transient inositol (1,4,5)trisphosphate accumulation under vasopressin stimulation in WRK1 cells: correlation with intracellular calcium mobilization</title><author>MOUILLAC, B ; BALESTRE, M.-N ; GUILLON, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-5c5ad8937730fb01c854df856254ec2fc10c5f3b7a4f143e2fbb2099c5c00aca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Arginine Vasopressin - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cell Line</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inositol 1,4,5-Trisphosphate</topic><topic>Inositol Phosphates - isolation & purification</topic><topic>Inositol Phosphates - metabolism</topic><topic>Kinetics</topic><topic>Mammary Neoplasms, Experimental</topic><topic>Proteins</topic><topic>Rats</topic><topic>Sugar Phosphates - metabolism</topic><topic>Type C Phospholipases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOUILLAC, B</creatorcontrib><creatorcontrib>BALESTRE, M.-N</creatorcontrib><creatorcontrib>GUILLON, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOUILLAC, B</au><au>BALESTRE, M.-N</au><au>GUILLON, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transient inositol (1,4,5)trisphosphate accumulation under vasopressin stimulation in WRK1 cells: correlation with intracellular calcium mobilization</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1989-03-31</date><risdate>1989</risdate><volume>159</volume><issue>3</issue><spage>953</spage><epage>960</epage><pages>953-960</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>In the rat mammary tumoral cell line (WRK1 cells), vasopressin was previously described to stimulate a phospholipase C. In this study, we have analysed the effect of vasopressin both on intracellular calcium mobilization and on the accumulation of inositol phosphates. Maximal concentration of vasopressin simultaneously induces an accumulation of Ins(1,4,5)P3 and a rise of intracellular calcium concentration. Both these two phenomena are transient and exhibit similar kinetics. A sustained accumulation of InsP2, Ins(1,3,4)P3 and InsP are observed later. Yet no stimulation of InsP4 can be objectified. These results indicate that Ins(1,4,5)P3 is the major inositol phosphate involved in intracellular calcium mobilization.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>2784680</pmid><doi>10.1016/0006-291X(89)92201-8</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1989-03, Vol.159 (3), p.953-960 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Arginine Vasopressin - pharmacology Biological and medical sciences Calcium - metabolism Cell Line Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Inositol 1,4,5-Trisphosphate Inositol Phosphates - isolation & purification Inositol Phosphates - metabolism Kinetics Mammary Neoplasms, Experimental Proteins Rats Sugar Phosphates - metabolism Type C Phospholipases - metabolism |
| title | Transient inositol (1,4,5)trisphosphate accumulation under vasopressin stimulation in WRK1 cells: correlation with intracellular calcium mobilization |
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