Adjuvanticity of an Ornithine-Containing Lipid of Flavobacterium meningosepticum as a Candidate Vaccine Adjuvant
Ornithine-containing lipids (OrnL) extracted from Flavobacterium meningosepticum have been reported to have various biological activities such as B-cell mitogenicity and macrophage activation to generate interleukin-1 and prostaglandin E2. We, using ovalbumin (OVA) as an antigen, evaluated the adjuv...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1997, Vol.41(2), pp.101-106 |
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| description | Ornithine-containing lipids (OrnL) extracted from Flavobacterium meningosepticum have been reported to have various biological activities such as B-cell mitogenicity and macrophage activation to generate interleukin-1 and prostaglandin E2. We, using ovalbumin (OVA) as an antigen, evaluated the adjuvant activity of OrnL as an immunological adjuvant in BALB/c mice. OrnL showed the function of forming liposome-like vesicles retaining biological activities when prepared as either small unilamellar or dehydration-rehydration vesicles. Although OrnL was not shown to have enough entrapping efficacy for use as a vaccine adjuvant, phosphatidylglycerol (PG) and cholesterol (CHOL) added to stabilize the vesicle membrane increased the entrapping efficacy to the same extent as that of conventional liposomes. Furthermore, the stabilized OrnL vesicles tolerated centrifugation to remove non-entrapped antigens. Completely antigen-entrapped OrnL vesicles including PG and CHOL induced a significantly greater enhancement of IgG antibody production than did aluminum hydroxide gel in BALB/c mice from week 6. These results indicate that OrnL can be utilized as an immunological adjuvant for vaccines. |
| doi_str_mv | 10.1111/j.1348-0421.1997.tb01175.x |
| format | Article |
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We, using ovalbumin (OVA) as an antigen, evaluated the adjuvant activity of OrnL as an immunological adjuvant in BALB/c mice. OrnL showed the function of forming liposome-like vesicles retaining biological activities when prepared as either small unilamellar or dehydration-rehydration vesicles. Although OrnL was not shown to have enough entrapping efficacy for use as a vaccine adjuvant, phosphatidylglycerol (PG) and cholesterol (CHOL) added to stabilize the vesicle membrane increased the entrapping efficacy to the same extent as that of conventional liposomes. Furthermore, the stabilized OrnL vesicles tolerated centrifugation to remove non-entrapped antigens. Completely antigen-entrapped OrnL vesicles including PG and CHOL induced a significantly greater enhancement of IgG antibody production than did aluminum hydroxide gel in BALB/c mice from week 6. These results indicate that OrnL can be utilized as an immunological adjuvant for vaccines.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1997.tb01175.x</identifier><identifier>PMID: 9087952</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Adjuvant ; Adjuvants, Immunologic - pharmacology ; Aluminum Hydroxide - immunology ; Animals ; Bacteriology ; Biological and medical sciences ; Cholesterol - pharmacology ; Dehydration-rehydration vesicle ; Female ; Flavobacterium - chemistry ; Flavobacterium meningosepticum ; Fundamental and applied biological sciences. Psychology ; Immunoglobulin G - analysis ; Immunoglobulin G - immunology ; Lipids - chemistry ; Lipids - immunology ; Lipids - isolation & purification ; Liposome ; Liposomes - immunology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Ornithine - immunology ; Ornithine - metabolism ; Ornithine-containing lipid ; Ovalbumin - immunology ; Phosphatidylglycerols - pharmacology ; Vaccination - methods ; Vaccines - administration & dosage ; Vaccines - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1997, Vol.41(2), pp.101-106</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6995-7a18894cf4efbe6c79eab098bed15b3faa6c851008c89c5112602eaa8ac025513</citedby><cites>FETCH-LOGICAL-c6995-7a18894cf4efbe6c79eab098bed15b3faa6c851008c89c5112602eaa8ac025513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1348-0421.1997.tb01175.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1348-0421.1997.tb01175.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,1882,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2615782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9087952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kato, Hiroshi</creatorcontrib><creatorcontrib>Goto, Norihisa</creatorcontrib><title>Adjuvanticity of an Ornithine-Containing Lipid of Flavobacterium meningosepticum as a Candidate Vaccine Adjuvant</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>Ornithine-containing lipids (OrnL) extracted from Flavobacterium meningosepticum have been reported to have various biological activities such as B-cell mitogenicity and macrophage activation to generate interleukin-1 and prostaglandin E2. We, using ovalbumin (OVA) as an antigen, evaluated the adjuvant activity of OrnL as an immunological adjuvant in BALB/c mice. OrnL showed the function of forming liposome-like vesicles retaining biological activities when prepared as either small unilamellar or dehydration-rehydration vesicles. Although OrnL was not shown to have enough entrapping efficacy for use as a vaccine adjuvant, phosphatidylglycerol (PG) and cholesterol (CHOL) added to stabilize the vesicle membrane increased the entrapping efficacy to the same extent as that of conventional liposomes. Furthermore, the stabilized OrnL vesicles tolerated centrifugation to remove non-entrapped antigens. Completely antigen-entrapped OrnL vesicles including PG and CHOL induced a significantly greater enhancement of IgG antibody production than did aluminum hydroxide gel in BALB/c mice from week 6. These results indicate that OrnL can be utilized as an immunological adjuvant for vaccines.</description><subject>Adjuvant</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Aluminum Hydroxide - immunology</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cholesterol - pharmacology</subject><subject>Dehydration-rehydration vesicle</subject><subject>Female</subject><subject>Flavobacterium - chemistry</subject><subject>Flavobacterium meningosepticum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin G - immunology</subject><subject>Lipids - chemistry</subject><subject>Lipids - immunology</subject><subject>Lipids - isolation & purification</subject><subject>Liposome</subject><subject>Liposomes - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Ornithine - immunology</subject><subject>Ornithine - metabolism</subject><subject>Ornithine-containing lipid</subject><subject>Ovalbumin - immunology</subject><subject>Phosphatidylglycerols - pharmacology</subject><subject>Vaccination - methods</subject><subject>Vaccines - administration & dosage</subject><subject>Vaccines - immunology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV1v0zAUhiMEGmXwE5AihBA3KT5pHNtcbWq3MtQxCfFxaZ04J5tL4pQ4He2_xyGl4gpELpxE7-PnWH6j6AWwKYTnzXoKs0wmLEthCkqJaV8wAMGnuwfR5Bg9jCZsJnnCc8YeR0-8XzOWilRmJ9GJYlIonk6izXm53t6j662x_T5uqxhdfNM5299ZR8m8dT1aZ91tvLIbWw7AZY33bYGmp85um7ihIW49bYIj_KOPMZ6jK22JPcVf0Jhgin_PeRo9qrD29OzwPo0-X158mr9LVjfLq_n5KjG5UjwRCFKqzFQZVQXlRijCgilZUAm8mFWIuZEcGJNGKsMB0pylhCjRsJRzmJ1Gr0bvpmu_b8n3urHeUF2jo3brtZCKZYrn_wQhZ6ByIQL4-u9gptIcGM8H9O2Imq71vqNKbzrbYLfXwPRQoV7roSc99KSHCvWhQr0Lm58f5myLhsrj1kNnIX95yNEbrKsOnbH-iIUzcCEH7GzEftia9v9xAH19df3rMyguRsXa93hLRwd2oeqadDO0DEoInYFOxwUYHHNzh50mFzzJ6LG-p90fmm86XFaY-fXDUsvF--ViMU_1x9lPgDjhPw</recordid><startdate>19970101</startdate><enddate>19970101</enddate><creator>Kato, Hiroshi</creator><creator>Goto, Norihisa</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7T7</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19970101</creationdate><title>Adjuvanticity of an Ornithine-Containing Lipid of Flavobacterium meningosepticum as a Candidate Vaccine Adjuvant</title><author>Kato, Hiroshi ; Goto, Norihisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6995-7a18894cf4efbe6c79eab098bed15b3faa6c851008c89c5112602eaa8ac025513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adjuvant</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Aluminum Hydroxide - immunology</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cholesterol - pharmacology</topic><topic>Dehydration-rehydration vesicle</topic><topic>Female</topic><topic>Flavobacterium - chemistry</topic><topic>Flavobacterium meningosepticum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin G - immunology</topic><topic>Lipids - chemistry</topic><topic>Lipids - immunology</topic><topic>Lipids - isolation & purification</topic><topic>Liposome</topic><topic>Liposomes - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Ornithine - immunology</topic><topic>Ornithine - metabolism</topic><topic>Ornithine-containing lipid</topic><topic>Ovalbumin - immunology</topic><topic>Phosphatidylglycerols - pharmacology</topic><topic>Vaccination - methods</topic><topic>Vaccines - administration & dosage</topic><topic>Vaccines - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kato, Hiroshi</creatorcontrib><creatorcontrib>Goto, Norihisa</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kato, Hiroshi</au><au>Goto, Norihisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adjuvanticity of an Ornithine-Containing Lipid of Flavobacterium meningosepticum as a Candidate Vaccine Adjuvant</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1997-01-01</date><risdate>1997</risdate><volume>41</volume><issue>2</issue><spage>101</spage><epage>106</epage><pages>101-106</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>Ornithine-containing lipids (OrnL) extracted from Flavobacterium meningosepticum have been reported to have various biological activities such as B-cell mitogenicity and macrophage activation to generate interleukin-1 and prostaglandin E2. We, using ovalbumin (OVA) as an antigen, evaluated the adjuvant activity of OrnL as an immunological adjuvant in BALB/c mice. OrnL showed the function of forming liposome-like vesicles retaining biological activities when prepared as either small unilamellar or dehydration-rehydration vesicles. Although OrnL was not shown to have enough entrapping efficacy for use as a vaccine adjuvant, phosphatidylglycerol (PG) and cholesterol (CHOL) added to stabilize the vesicle membrane increased the entrapping efficacy to the same extent as that of conventional liposomes. Furthermore, the stabilized OrnL vesicles tolerated centrifugation to remove non-entrapped antigens. Completely antigen-entrapped OrnL vesicles including PG and CHOL induced a significantly greater enhancement of IgG antibody production than did aluminum hydroxide gel in BALB/c mice from week 6. These results indicate that OrnL can be utilized as an immunological adjuvant for vaccines.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>9087952</pmid><doi>10.1111/j.1348-0421.1997.tb01175.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-5600 |
| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1997, Vol.41(2), pp.101-106 |
| issn | 0385-5600 1348-0421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78904956 |
| source | J-STAGE Free; MEDLINE; Wiley Free Content; Freely Accessible Japanese Titles; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Adjuvant Adjuvants, Immunologic - pharmacology Aluminum Hydroxide - immunology Animals Bacteriology Biological and medical sciences Cholesterol - pharmacology Dehydration-rehydration vesicle Female Flavobacterium - chemistry Flavobacterium meningosepticum Fundamental and applied biological sciences. Psychology Immunoglobulin G - analysis Immunoglobulin G - immunology Lipids - chemistry Lipids - immunology Lipids - isolation & purification Liposome Liposomes - immunology Mice Mice, Inbred BALB C Microbiology Ornithine - immunology Ornithine - metabolism Ornithine-containing lipid Ovalbumin - immunology Phosphatidylglycerols - pharmacology Vaccination - methods Vaccines - administration & dosage Vaccines - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies |
| title | Adjuvanticity of an Ornithine-Containing Lipid of Flavobacterium meningosepticum as a Candidate Vaccine Adjuvant |
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