nm23—Relationship to the metastatic potential of breast carcinoma cell lines, primary human xenografts, and lymph node negative breast carcinoma patients
BACKGROUND Since the discovery of nm23 (nonmetastatic) by Steeg et al. in 1988, a number of tumor cohort studies have shown an inverse relationship between the levels of expression of the nm23‐H1 protein and disease aggressiveness and tumor metastatic potential. METHODS The relationship between the...
Gespeichert in:
| Veröffentlicht in: | Cancer 1997-03, Vol.79 (6), p.1158-1165 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1165 |
|---|---|
| container_issue | 6 |
| container_start_page | 1158 |
| container_title | Cancer |
| container_volume | 79 |
| creator | Russell, Rosalind Lee Geisinger, Kim R. Mehta, Rajeshwari R. White, Wain L. Shelton, Brent Kute, Timothy Edward |
| description | BACKGROUND
Since the discovery of nm23 (nonmetastatic) by Steeg et al. in 1988, a number of tumor cohort studies have shown an inverse relationship between the levels of expression of the nm23‐H1 protein and disease aggressiveness and tumor metastatic potential.
METHODS
The relationship between the expression of nm23 protein and the metastatic potential of human breast carcinoma was analyzed in cell lines, xenografts, and in a retrospective lymph node negative breast carcinoma population. The lymph node negative breast carcinoma study was comprised of 40 patients: 19 with nonrecurrent and 21 with recurrent disease. The 40 patients were matched according to age, cathepsin D, tumor size, percent S‐phase, DNA ploidy, steroid receptor status, and tumor grade. Nm23‐H1 protein levels in cell lines and xenografts were analyzed quantitatively using Western blot analyses and semiquantitatively in tissue sections using immunocytochemistry. Immunocytochemical analysis of lymph node negative breast tumors was graded as the percent of tumor staining positive for nm23 and the intensity of staining. The metastatic potentials of the cell lines and xenografts were assessed as the ability to form metastatic lesions in nude mice. In the lymph node negative breast carcinoma patients, the metastatic potential was characterized as the incidence of breast carcinoma recurrence.
RESULTS
The MCF‐7 cell line expressed four‐ and tenfold higher levels of nm23‐H1 than the highly metastatic MDA‐MB‐435 and MDA‐MB‐231 cells, respectively. Among the xenografts and cell lines, there was an inverse correlation between nm23‐H1 expression and metastatic potential in athymic nude mice (correlation coefficient [R] = ‐0.51). The differences between the levels of nm23‐H1 among the metastatic and nonmetastatic cell lines and xenografts were not statistically significant. Statistical analyses indicated that neither the intensity nor the percent of tumor staining positive for nm23 expression was correlated to the recurrence of breast carcinoma in the lymph node negative patient population that had been matched for other clinical prognostic markers.
CONCLUSIONS
There was an inverse correlation (R = ‐0.51) between the levels of nm23‐H1 expression in cell lines and xenografts and the metastatic potential in nude mice. In the retrospective lymph node negative breast carcinoma population, no clear association was demonstrated between the expression of nm23 and breast carcinoma recurrence. This observation |
| doi_str_mv | 10.1002/(SICI)1097-0142(19970315)79:6<1158::AID-CNCR14>3.0.CO;2-Z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78900435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78900435</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3334-80fc2c55e2d710f177e42e3b195219e5ef77116a9dbfab8d3f9a33d06f18af863</originalsourceid><addsrcrecordid>eNqFkd-K1DAYxYso6-zqIwi5ENkFO-ZP2zSjLAz138DiwKqg68VH2n7ZqbRpbTrq3PkQ3vl2PokpU-dmBS9CyPlOTk74BcGS0TmjlD85fbvKVmeMKhlSFvFTppSkgsVnUi2SZ4zF6WKxXD0PszfZJYvOxZzOs_VTHl7dCmaHW7eDGaU0DeNIfLgbHDv32R8lj8VRcKSopJESs-CXbbj4_ePnJdZ6qFrrNlVHhpYMGyQNDtoNXi5I1w5oh0rXpDUk79HrpNB9Udm20aTAuiZ1ZdE9Jl1fNbrfkc220ZZ8R9te99oMfqJtSepd022IbUskFq999Fe8Gdd53b_m7gV3jK4d3p_2k-D9yxfvstfhxfrVKltehIUQIgpTagpexDHyUjJqmJQYcRQ5UzFnCmM0UjKWaFXmRudpKYzSQpQ0MSzVJk3ESfBon9v17ZctugGayo1_0hbbrQOZKkojEXvjx72x6FvnejQw_RYYhREcwAgORgQwIoC_4EAqSGAEB-DBwR4cCKCQrYHDlc9-MJXY5g2Wh-SJlJ8_nObaFbo2vbZF5Q42ngjll7d92tu-VTXubvT7f71_tpsU8QfWb8Yc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78900435</pqid></control><display><type>article</type><title>nm23—Relationship to the metastatic potential of breast carcinoma cell lines, primary human xenografts, and lymph node negative breast carcinoma patients</title><source>MEDLINE</source><source>Wiley Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley Online Library (Open Access Collection)</source><source>Alma/SFX Local Collection</source><creator>Russell, Rosalind Lee ; Geisinger, Kim R. ; Mehta, Rajeshwari R. ; White, Wain L. ; Shelton, Brent ; Kute, Timothy Edward</creator><creatorcontrib>Russell, Rosalind Lee ; Geisinger, Kim R. ; Mehta, Rajeshwari R. ; White, Wain L. ; Shelton, Brent ; Kute, Timothy Edward</creatorcontrib><description>BACKGROUND
Since the discovery of nm23 (nonmetastatic) by Steeg et al. in 1988, a number of tumor cohort studies have shown an inverse relationship between the levels of expression of the nm23‐H1 protein and disease aggressiveness and tumor metastatic potential.
METHODS
The relationship between the expression of nm23 protein and the metastatic potential of human breast carcinoma was analyzed in cell lines, xenografts, and in a retrospective lymph node negative breast carcinoma population. The lymph node negative breast carcinoma study was comprised of 40 patients: 19 with nonrecurrent and 21 with recurrent disease. The 40 patients were matched according to age, cathepsin D, tumor size, percent S‐phase, DNA ploidy, steroid receptor status, and tumor grade. Nm23‐H1 protein levels in cell lines and xenografts were analyzed quantitatively using Western blot analyses and semiquantitatively in tissue sections using immunocytochemistry. Immunocytochemical analysis of lymph node negative breast tumors was graded as the percent of tumor staining positive for nm23 and the intensity of staining. The metastatic potentials of the cell lines and xenografts were assessed as the ability to form metastatic lesions in nude mice. In the lymph node negative breast carcinoma patients, the metastatic potential was characterized as the incidence of breast carcinoma recurrence.
RESULTS
The MCF‐7 cell line expressed four‐ and tenfold higher levels of nm23‐H1 than the highly metastatic MDA‐MB‐435 and MDA‐MB‐231 cells, respectively. Among the xenografts and cell lines, there was an inverse correlation between nm23‐H1 expression and metastatic potential in athymic nude mice (correlation coefficient [R] = ‐0.51). The differences between the levels of nm23‐H1 among the metastatic and nonmetastatic cell lines and xenografts were not statistically significant. Statistical analyses indicated that neither the intensity nor the percent of tumor staining positive for nm23 expression was correlated to the recurrence of breast carcinoma in the lymph node negative patient population that had been matched for other clinical prognostic markers.
CONCLUSIONS
There was an inverse correlation (R = ‐0.51) between the levels of nm23‐H1 expression in cell lines and xenografts and the metastatic potential in nude mice. In the retrospective lymph node negative breast carcinoma population, no clear association was demonstrated between the expression of nm23 and breast carcinoma recurrence. This observation suggests the nm23 expression does not predict outcome in lymph node negative breast carcinoma patients. Cancer 1997; 79:1158‐65. © 1997 American Cancer Society.
Analyses of the levels of nm23 protein were performed on breast tumor cell lines, xenografts, and a retrospective lymph node negative breast carcinoma population using immunohistochemical and/or Western blot methods. No significant correlation was observed between the levels of nm23 and the metastatic potential of these breast tumor populations.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/(SICI)1097-0142(19970315)79:6<1158::AID-CNCR14>3.0.CO;2-Z</identifier><identifier>PMID: 9070493</identifier><identifier>CODEN: CANCAR</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Aged ; Animals ; Biological and medical sciences ; Blotting, Western ; Breast ; Breast carcinoma ; Breast Neoplasms - chemistry ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; clinical ; Female ; Gene Expression Regulation, Neoplastic ; Gynecology. Andrology. Obstetrics ; Humans ; immunohistochemistry ; lymph node‐negative ; Lymphatic Metastasis ; Mammary gland diseases ; Medical sciences ; metastasis ; Mice ; Mice, Nude ; Middle Aged ; Monomeric GTP-Binding Proteins ; Neoplasm Metastasis ; Neoplasm Recurrence, Local - chemistry ; Neoplasm Recurrence, Local - genetics ; nm23 ; NM23 Nucleoside Diphosphate Kinases ; Nucleoside-Diphosphate Kinase ; Prognosis ; Transcription Factors - analysis ; Transcription Factors - genetics ; Transplantation, Heterologous ; Tumor Cells, Cultured - pathology ; Tumors</subject><ispartof>Cancer, 1997-03, Vol.79 (6), p.1158-1165</ispartof><rights>Copyright © 1997 American Cancer Society</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3334-80fc2c55e2d710f177e42e3b195219e5ef77116a9dbfab8d3f9a33d06f18af863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-0142%2819970315%2979%3A6%3C1158%3A%3AAID-CNCR14%3E3.0.CO%3B2-Z$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-0142%2819970315%2979%3A6%3C1158%3A%3AAID-CNCR14%3E3.0.CO%3B2-Z$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2639263$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9070493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Russell, Rosalind Lee</creatorcontrib><creatorcontrib>Geisinger, Kim R.</creatorcontrib><creatorcontrib>Mehta, Rajeshwari R.</creatorcontrib><creatorcontrib>White, Wain L.</creatorcontrib><creatorcontrib>Shelton, Brent</creatorcontrib><creatorcontrib>Kute, Timothy Edward</creatorcontrib><title>nm23—Relationship to the metastatic potential of breast carcinoma cell lines, primary human xenografts, and lymph node negative breast carcinoma patients</title><title>Cancer</title><addtitle>Cancer</addtitle><description>BACKGROUND
Since the discovery of nm23 (nonmetastatic) by Steeg et al. in 1988, a number of tumor cohort studies have shown an inverse relationship between the levels of expression of the nm23‐H1 protein and disease aggressiveness and tumor metastatic potential.
METHODS
The relationship between the expression of nm23 protein and the metastatic potential of human breast carcinoma was analyzed in cell lines, xenografts, and in a retrospective lymph node negative breast carcinoma population. The lymph node negative breast carcinoma study was comprised of 40 patients: 19 with nonrecurrent and 21 with recurrent disease. The 40 patients were matched according to age, cathepsin D, tumor size, percent S‐phase, DNA ploidy, steroid receptor status, and tumor grade. Nm23‐H1 protein levels in cell lines and xenografts were analyzed quantitatively using Western blot analyses and semiquantitatively in tissue sections using immunocytochemistry. Immunocytochemical analysis of lymph node negative breast tumors was graded as the percent of tumor staining positive for nm23 and the intensity of staining. The metastatic potentials of the cell lines and xenografts were assessed as the ability to form metastatic lesions in nude mice. In the lymph node negative breast carcinoma patients, the metastatic potential was characterized as the incidence of breast carcinoma recurrence.
RESULTS
The MCF‐7 cell line expressed four‐ and tenfold higher levels of nm23‐H1 than the highly metastatic MDA‐MB‐435 and MDA‐MB‐231 cells, respectively. Among the xenografts and cell lines, there was an inverse correlation between nm23‐H1 expression and metastatic potential in athymic nude mice (correlation coefficient [R] = ‐0.51). The differences between the levels of nm23‐H1 among the metastatic and nonmetastatic cell lines and xenografts were not statistically significant. Statistical analyses indicated that neither the intensity nor the percent of tumor staining positive for nm23 expression was correlated to the recurrence of breast carcinoma in the lymph node negative patient population that had been matched for other clinical prognostic markers.
CONCLUSIONS
There was an inverse correlation (R = ‐0.51) between the levels of nm23‐H1 expression in cell lines and xenografts and the metastatic potential in nude mice. In the retrospective lymph node negative breast carcinoma population, no clear association was demonstrated between the expression of nm23 and breast carcinoma recurrence. This observation suggests the nm23 expression does not predict outcome in lymph node negative breast carcinoma patients. Cancer 1997; 79:1158‐65. © 1997 American Cancer Society.
Analyses of the levels of nm23 protein were performed on breast tumor cell lines, xenografts, and a retrospective lymph node negative breast carcinoma population using immunohistochemical and/or Western blot methods. No significant correlation was observed between the levels of nm23 and the metastatic potential of these breast tumor populations.</description><subject>Aged</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Breast</subject><subject>Breast carcinoma</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>clinical</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>immunohistochemistry</subject><subject>lymph node‐negative</subject><subject>Lymphatic Metastasis</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>metastasis</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>Monomeric GTP-Binding Proteins</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Recurrence, Local - chemistry</subject><subject>Neoplasm Recurrence, Local - genetics</subject><subject>nm23</subject><subject>NM23 Nucleoside Diphosphate Kinases</subject><subject>Nucleoside-Diphosphate Kinase</subject><subject>Prognosis</subject><subject>Transcription Factors - analysis</subject><subject>Transcription Factors - genetics</subject><subject>Transplantation, Heterologous</subject><subject>Tumor Cells, Cultured - pathology</subject><subject>Tumors</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-K1DAYxYso6-zqIwi5ENkFO-ZP2zSjLAz138DiwKqg68VH2n7ZqbRpbTrq3PkQ3vl2PokpU-dmBS9CyPlOTk74BcGS0TmjlD85fbvKVmeMKhlSFvFTppSkgsVnUi2SZ4zF6WKxXD0PszfZJYvOxZzOs_VTHl7dCmaHW7eDGaU0DeNIfLgbHDv32R8lj8VRcKSopJESs-CXbbj4_ePnJdZ6qFrrNlVHhpYMGyQNDtoNXi5I1w5oh0rXpDUk79HrpNB9Udm20aTAuiZ1ZdE9Jl1fNbrfkc220ZZ8R9te99oMfqJtSepd022IbUskFq999Fe8Gdd53b_m7gV3jK4d3p_2k-D9yxfvstfhxfrVKltehIUQIgpTagpexDHyUjJqmJQYcRQ5UzFnCmM0UjKWaFXmRudpKYzSQpQ0MSzVJk3ESfBon9v17ZctugGayo1_0hbbrQOZKkojEXvjx72x6FvnejQw_RYYhREcwAgORgQwIoC_4EAqSGAEB-DBwR4cCKCQrYHDlc9-MJXY5g2Wh-SJlJ8_nObaFbo2vbZF5Q42ngjll7d92tu-VTXubvT7f71_tpsU8QfWb8Yc</recordid><startdate>19970315</startdate><enddate>19970315</enddate><creator>Russell, Rosalind Lee</creator><creator>Geisinger, Kim R.</creator><creator>Mehta, Rajeshwari R.</creator><creator>White, Wain L.</creator><creator>Shelton, Brent</creator><creator>Kute, Timothy Edward</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970315</creationdate><title>nm23—Relationship to the metastatic potential of breast carcinoma cell lines, primary human xenografts, and lymph node negative breast carcinoma patients</title><author>Russell, Rosalind Lee ; Geisinger, Kim R. ; Mehta, Rajeshwari R. ; White, Wain L. ; Shelton, Brent ; Kute, Timothy Edward</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3334-80fc2c55e2d710f177e42e3b195219e5ef77116a9dbfab8d3f9a33d06f18af863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Aged</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Breast</topic><topic>Breast carcinoma</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>clinical</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>immunohistochemistry</topic><topic>lymph node‐negative</topic><topic>Lymphatic Metastasis</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>metastasis</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>Monomeric GTP-Binding Proteins</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Recurrence, Local - chemistry</topic><topic>Neoplasm Recurrence, Local - genetics</topic><topic>nm23</topic><topic>NM23 Nucleoside Diphosphate Kinases</topic><topic>Nucleoside-Diphosphate Kinase</topic><topic>Prognosis</topic><topic>Transcription Factors - analysis</topic><topic>Transcription Factors - genetics</topic><topic>Transplantation, Heterologous</topic><topic>Tumor Cells, Cultured - pathology</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Russell, Rosalind Lee</creatorcontrib><creatorcontrib>Geisinger, Kim R.</creatorcontrib><creatorcontrib>Mehta, Rajeshwari R.</creatorcontrib><creatorcontrib>White, Wain L.</creatorcontrib><creatorcontrib>Shelton, Brent</creatorcontrib><creatorcontrib>Kute, Timothy Edward</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Russell, Rosalind Lee</au><au>Geisinger, Kim R.</au><au>Mehta, Rajeshwari R.</au><au>White, Wain L.</au><au>Shelton, Brent</au><au>Kute, Timothy Edward</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>nm23—Relationship to the metastatic potential of breast carcinoma cell lines, primary human xenografts, and lymph node negative breast carcinoma patients</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>1997-03-15</date><risdate>1997</risdate><volume>79</volume><issue>6</issue><spage>1158</spage><epage>1165</epage><pages>1158-1165</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><coden>CANCAR</coden><abstract>BACKGROUND
Since the discovery of nm23 (nonmetastatic) by Steeg et al. in 1988, a number of tumor cohort studies have shown an inverse relationship between the levels of expression of the nm23‐H1 protein and disease aggressiveness and tumor metastatic potential.
METHODS
The relationship between the expression of nm23 protein and the metastatic potential of human breast carcinoma was analyzed in cell lines, xenografts, and in a retrospective lymph node negative breast carcinoma population. The lymph node negative breast carcinoma study was comprised of 40 patients: 19 with nonrecurrent and 21 with recurrent disease. The 40 patients were matched according to age, cathepsin D, tumor size, percent S‐phase, DNA ploidy, steroid receptor status, and tumor grade. Nm23‐H1 protein levels in cell lines and xenografts were analyzed quantitatively using Western blot analyses and semiquantitatively in tissue sections using immunocytochemistry. Immunocytochemical analysis of lymph node negative breast tumors was graded as the percent of tumor staining positive for nm23 and the intensity of staining. The metastatic potentials of the cell lines and xenografts were assessed as the ability to form metastatic lesions in nude mice. In the lymph node negative breast carcinoma patients, the metastatic potential was characterized as the incidence of breast carcinoma recurrence.
RESULTS
The MCF‐7 cell line expressed four‐ and tenfold higher levels of nm23‐H1 than the highly metastatic MDA‐MB‐435 and MDA‐MB‐231 cells, respectively. Among the xenografts and cell lines, there was an inverse correlation between nm23‐H1 expression and metastatic potential in athymic nude mice (correlation coefficient [R] = ‐0.51). The differences between the levels of nm23‐H1 among the metastatic and nonmetastatic cell lines and xenografts were not statistically significant. Statistical analyses indicated that neither the intensity nor the percent of tumor staining positive for nm23 expression was correlated to the recurrence of breast carcinoma in the lymph node negative patient population that had been matched for other clinical prognostic markers.
CONCLUSIONS
There was an inverse correlation (R = ‐0.51) between the levels of nm23‐H1 expression in cell lines and xenografts and the metastatic potential in nude mice. In the retrospective lymph node negative breast carcinoma population, no clear association was demonstrated between the expression of nm23 and breast carcinoma recurrence. This observation suggests the nm23 expression does not predict outcome in lymph node negative breast carcinoma patients. Cancer 1997; 79:1158‐65. © 1997 American Cancer Society.
Analyses of the levels of nm23 protein were performed on breast tumor cell lines, xenografts, and a retrospective lymph node negative breast carcinoma population using immunohistochemical and/or Western blot methods. No significant correlation was observed between the levels of nm23 and the metastatic potential of these breast tumor populations.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>9070493</pmid><doi>10.1002/(SICI)1097-0142(19970315)79:6<1158::AID-CNCR14>3.0.CO;2-Z</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-543X |
| ispartof | Cancer, 1997-03, Vol.79 (6), p.1158-1165 |
| issn | 0008-543X 1097-0142 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78900435 |
| source | MEDLINE; Wiley Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Aged Animals Biological and medical sciences Blotting, Western Breast Breast carcinoma Breast Neoplasms - chemistry Breast Neoplasms - genetics Breast Neoplasms - pathology clinical Female Gene Expression Regulation, Neoplastic Gynecology. Andrology. Obstetrics Humans immunohistochemistry lymph node‐negative Lymphatic Metastasis Mammary gland diseases Medical sciences metastasis Mice Mice, Nude Middle Aged Monomeric GTP-Binding Proteins Neoplasm Metastasis Neoplasm Recurrence, Local - chemistry Neoplasm Recurrence, Local - genetics nm23 NM23 Nucleoside Diphosphate Kinases Nucleoside-Diphosphate Kinase Prognosis Transcription Factors - analysis Transcription Factors - genetics Transplantation, Heterologous Tumor Cells, Cultured - pathology Tumors |
| title | nm23—Relationship to the metastatic potential of breast carcinoma cell lines, primary human xenografts, and lymph node negative breast carcinoma patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T22%3A36%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=nm23%E2%80%94Relationship%20to%20the%20metastatic%20potential%20of%20breast%20carcinoma%20cell%20lines,%20primary%20human%20xenografts,%20and%20lymph%20node%20negative%20breast%20carcinoma%20patients&rft.jtitle=Cancer&rft.au=Russell,%20Rosalind%20Lee&rft.date=1997-03-15&rft.volume=79&rft.issue=6&rft.spage=1158&rft.epage=1165&rft.pages=1158-1165&rft.issn=0008-543X&rft.eissn=1097-0142&rft.coden=CANCAR&rft_id=info:doi/10.1002/(SICI)1097-0142(19970315)79:6%3C1158::AID-CNCR14%3E3.0.CO;2-Z&rft_dat=%3Cproquest_cross%3E78900435%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78900435&rft_id=info:pmid/9070493&rfr_iscdi=true |