Localization of leptin receptor mRNA splice variants in murine peripheral tissues by RT-PCR and in situ hybridization
Expression of leptin receptor splice variants, including the long form variant (Ob-Rb), has been examined in murine peripheral tissues. RT-PCR indicates that the leptin receptor, Ob-R, and in particular the Ob-Ra splice variant are expressed in a wide range of tissues. Expression of the Ob-R recepto...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1997-03, Vol.232 (2), p.383-387 |
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| creator | Hoggard, N Mercer, J G Rayner, D V Moar, K Trayhurn, P Williams, L M |
| description | Expression of leptin receptor splice variants, including the long form variant (Ob-Rb), has been examined in murine peripheral tissues. RT-PCR indicates that the leptin receptor, Ob-R, and in particular the Ob-Ra splice variant are expressed in a wide range of tissues. Expression of the Ob-R receptor was localized by in situ hybridization to specific sites in the spleen, testes, kidney, liver, lung, and adrenal. However, the long form leptin receptor, Ob-Rb, was only expressed at significant levels in the medulla of the adrenal and the inner zone of the medulla of the kidney. The specific sites of expression of different splice variants within peripheral tissues has important implications with regard to the function of leptin. |
| doi_str_mv | 10.1006/bbrc.1997.6245 |
| format | Article |
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RT-PCR indicates that the leptin receptor, Ob-R, and in particular the Ob-Ra splice variant are expressed in a wide range of tissues. Expression of the Ob-R receptor was localized by in situ hybridization to specific sites in the spleen, testes, kidney, liver, lung, and adrenal. However, the long form leptin receptor, Ob-Rb, was only expressed at significant levels in the medulla of the adrenal and the inner zone of the medulla of the kidney. 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RT-PCR indicates that the leptin receptor, Ob-R, and in particular the Ob-Ra splice variant are expressed in a wide range of tissues. Expression of the Ob-R receptor was localized by in situ hybridization to specific sites in the spleen, testes, kidney, liver, lung, and adrenal. However, the long form leptin receptor, Ob-Rb, was only expressed at significant levels in the medulla of the adrenal and the inner zone of the medulla of the kidney. The specific sites of expression of different splice variants within peripheral tissues has important implications with regard to the function of leptin.</description><subject>Animals</subject><subject>Carrier Proteins - genetics</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Receptors, Cell Surface</subject><subject>Receptors, Cytokine - genetics</subject><subject>Receptors, Leptin</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-Directed DNA Polymerase</subject><issn>0006-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkM1LAzEQxXNQaq1evQk5eduaZJts5liKVqGolArelmSTpZHsh0lWqH-9K-5phuH35j0eQjeULCkh4l7rUC0pQLEUbMXP0JyM14wB_bhAlzF-EkLpSsAMzYAyTqWYo2HXVcq7H5Vc1-Kuxt72ybU42GpcuoCb_csax967yuJvFZxqU8Qj0AzBtRb3Nrj-aIPyOLkYBxuxPuH9IXvb7LFqzR8aXRrw8aSDM5PRFTqvlY_2epoL9P74cNg8ZbvX7fNmvct6RkTKaCEkAylzozWsTM6k4EQVVitOihpUTQUHIIJLALCMSSqNNEAYqXmteJUv0N3_3z50X2O2VDYuVtZ71dpuiGUhRyWn-QjeTuCgG2vKPrhGhVM59ZT_ArpAaSU</recordid><startdate>19970317</startdate><enddate>19970317</enddate><creator>Hoggard, N</creator><creator>Mercer, J G</creator><creator>Rayner, D V</creator><creator>Moar, K</creator><creator>Trayhurn, P</creator><creator>Williams, L M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970317</creationdate><title>Localization of leptin receptor mRNA splice variants in murine peripheral tissues by RT-PCR and in situ hybridization</title><author>Hoggard, N ; Mercer, J G ; Rayner, D V ; Moar, K ; Trayhurn, P ; Williams, L M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p206t-176829883dbb94d328650a7eba507f9af165990658999e22818d8d9020f5fa5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Carrier Proteins - genetics</topic><topic>In Situ Hybridization</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Receptors, Cell Surface</topic><topic>Receptors, Cytokine - genetics</topic><topic>Receptors, Leptin</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-Directed DNA Polymerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoggard, N</creatorcontrib><creatorcontrib>Mercer, J G</creatorcontrib><creatorcontrib>Rayner, D V</creatorcontrib><creatorcontrib>Moar, K</creatorcontrib><creatorcontrib>Trayhurn, P</creatorcontrib><creatorcontrib>Williams, L M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoggard, N</au><au>Mercer, J G</au><au>Rayner, D V</au><au>Moar, K</au><au>Trayhurn, P</au><au>Williams, L M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of leptin receptor mRNA splice variants in murine peripheral tissues by RT-PCR and in situ hybridization</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1997-03-17</date><risdate>1997</risdate><volume>232</volume><issue>2</issue><spage>383</spage><epage>387</epage><pages>383-387</pages><issn>0006-291X</issn><abstract>Expression of leptin receptor splice variants, including the long form variant (Ob-Rb), has been examined in murine peripheral tissues. 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| ispartof | Biochemical and biophysical research communications, 1997-03, Vol.232 (2), p.383-387 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Carrier Proteins - genetics In Situ Hybridization Mice Molecular Sequence Data Organ Specificity - genetics Polymerase Chain Reaction Receptors, Cell Surface Receptors, Cytokine - genetics Receptors, Leptin RNA Splicing RNA, Messenger - metabolism RNA-Directed DNA Polymerase |
| title | Localization of leptin receptor mRNA splice variants in murine peripheral tissues by RT-PCR and in situ hybridization |
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