Cloning and Expression of a Xenopus Embryonic Gap Junction Protein

Gap junctions in the early amphibian embryo may play a fundamental role in the regulation of differentiation by mediating the cell-to-cell transfer of chemical signals. A complementary DNA encoding a gap junction present in Xenopus oocytes and early embryos has now been cloned and sequenced. This pr...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1989-03, Vol.243 (4895), p.1194-1195
Hauptverfasser: Ebihara, L., Beyer, E. C., Swenson, K. I., Paul, D. L., Goodenough, D. A.
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container_issue 4895
container_start_page 1194
container_title Science (American Association for the Advancement of Science)
container_volume 243
creator Ebihara, L.
Beyer, E. C.
Swenson, K. I.
Paul, D. L.
Goodenough, D. A.
description Gap junctions in the early amphibian embryo may play a fundamental role in the regulation of differentiation by mediating the cell-to-cell transfer of chemical signals. A complementary DNA encoding a gap junction present in Xenopus oocytes and early embryos has now been cloned and sequenced. This protein sequence is homologous to the well-characterized gap junction structural proteins rat connexin32 and connexin43. RNA blot analysis of total Xenopus oocyte RNA showed hybridization to a single 1.6-kilobase band. This messenger RNA is abundant in oocytes, decreases to levels below the sensitivity of our assay by stage 15 (18 hours), and is not detectable in RNA from a number of adult organs. To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. Pairs of RNA-injected oocytes formed many more time- and voltage-sensitive cell-cell channels than water-injected pairs.
doi_str_mv 10.1126/science.2466337
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subjects Amino Acid Sequence
Animals
Biochemistry
Biological and medical sciences
Cell Communication
Cell junctions
Cloning, Molecular
Complementary DNA
Connexins
DNA Probes
Electric Conductivity
Electric potential
Embryos
Female
Fetuses
Fundamental and applied biological sciences. Psychology
Gap junctions
Gene expression
Gene Expression Regulation
genes
Genetics
Granulosa cells
Intercellular Junctions - physiology
Junctional complexes (Epithelium)
Membrane Proteins - genetics
Membrane Proteins - physiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Oocytes
Oocytes - analysis
Oocytes - physiology
Ovaries
Rats
RNA
RNA - analysis
RNA, Messenger - analysis
Tissue Distribution
Voltage-clamp technique (Electrophysiology)
Xenopus
Xenopus - embryology
title Cloning and Expression of a Xenopus Embryonic Gap Junction Protein
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