Cloning and Expression of a Xenopus Embryonic Gap Junction Protein
Gap junctions in the early amphibian embryo may play a fundamental role in the regulation of differentiation by mediating the cell-to-cell transfer of chemical signals. A complementary DNA encoding a gap junction present in Xenopus oocytes and early embryos has now been cloned and sequenced. This pr...
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Veröffentlicht in: | Science (American Association for the Advancement of Science) 1989-03, Vol.243 (4895), p.1194-1195 |
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creator | Ebihara, L. Beyer, E. C. Swenson, K. I. Paul, D. L. Goodenough, D. A. |
description | Gap junctions in the early amphibian embryo may play a fundamental role in the regulation of differentiation by mediating the cell-to-cell transfer of chemical signals. A complementary DNA encoding a gap junction present in Xenopus oocytes and early embryos has now been cloned and sequenced. This protein sequence is homologous to the well-characterized gap junction structural proteins rat connexin32 and connexin43. RNA blot analysis of total Xenopus oocyte RNA showed hybridization to a single 1.6-kilobase band. This messenger RNA is abundant in oocytes, decreases to levels below the sensitivity of our assay by stage 15 (18 hours), and is not detectable in RNA from a number of adult organs. To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. Pairs of RNA-injected oocytes formed many more time- and voltage-sensitive cell-cell channels than water-injected pairs. |
doi_str_mv | 10.1126/science.2466337 |
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To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. Pairs of RNA-injected oocytes formed many more time- and voltage-sensitive cell-cell channels than water-injected pairs.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.2466337</identifier><identifier>PMID: 2466337</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>Amino Acid Sequence ; Animals ; Biochemistry ; Biological and medical sciences ; Cell Communication ; Cell junctions ; Cloning, Molecular ; Complementary DNA ; Connexins ; DNA Probes ; Electric Conductivity ; Electric potential ; Embryos ; Female ; Fetuses ; Fundamental and applied biological sciences. Psychology ; Gap junctions ; Gene expression ; Gene Expression Regulation ; genes ; Genetics ; Granulosa cells ; Intercellular Junctions - physiology ; Junctional complexes (Epithelium) ; Membrane Proteins - genetics ; Membrane Proteins - physiology ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Oocytes ; Oocytes - analysis ; Oocytes - physiology ; Ovaries ; Rats ; RNA ; RNA - analysis ; RNA, Messenger - analysis ; Tissue Distribution ; Voltage-clamp technique (Electrophysiology) ; Xenopus ; Xenopus - embryology</subject><ispartof>Science (American Association for the Advancement of Science), 1989-03, Vol.243 (4895), p.1194-1195</ispartof><rights>Copyright 1989 The American Association for the Advancement of Science</rights><rights>1989 INIST-CNRS</rights><rights>COPYRIGHT 1989 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1989 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Mar 3, 1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c730t-72f77f11a74f1075186853be39b20b4d3179a408a9580e69552402fd35089e3</citedby><cites>FETCH-LOGICAL-c730t-72f77f11a74f1075186853be39b20b4d3179a408a9580e69552402fd35089e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1702842$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1702842$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,778,782,801,2873,2874,27913,27914,58006,58239</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7373603$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2466337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebihara, L.</creatorcontrib><creatorcontrib>Beyer, E. C.</creatorcontrib><creatorcontrib>Swenson, K. I.</creatorcontrib><creatorcontrib>Paul, D. L.</creatorcontrib><creatorcontrib>Goodenough, D. A.</creatorcontrib><title>Cloning and Expression of a Xenopus Embryonic Gap Junction Protein</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Gap junctions in the early amphibian embryo may play a fundamental role in the regulation of differentiation by mediating the cell-to-cell transfer of chemical signals. A complementary DNA encoding a gap junction present in Xenopus oocytes and early embryos has now been cloned and sequenced. This protein sequence is homologous to the well-characterized gap junction structural proteins rat connexin32 and connexin43. RNA blot analysis of total Xenopus oocyte RNA showed hybridization to a single 1.6-kilobase band. This messenger RNA is abundant in oocytes, decreases to levels below the sensitivity of our assay by stage 15 (18 hours), and is not detectable in RNA from a number of adult organs. To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. Pairs of RNA-injected oocytes formed many more time- and voltage-sensitive cell-cell channels than water-injected pairs.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cell Communication</subject><subject>Cell junctions</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>Connexins</subject><subject>DNA Probes</subject><subject>Electric Conductivity</subject><subject>Electric potential</subject><subject>Embryos</subject><subject>Female</subject><subject>Fetuses</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gap junctions</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>Genetics</subject><subject>Granulosa cells</subject><subject>Intercellular Junctions - physiology</subject><subject>Junctional complexes (Epithelium)</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - physiology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Oocytes</subject><subject>Oocytes - analysis</subject><subject>Oocytes - physiology</subject><subject>Ovaries</subject><subject>Rats</subject><subject>RNA</subject><subject>RNA - analysis</subject><subject>RNA, Messenger - analysis</subject><subject>Tissue Distribution</subject><subject>Voltage-clamp technique (Electrophysiology)</subject><subject>Xenopus</subject><subject>Xenopus - embryology</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN082L1DAUAPAiyjqunr0oFBE9rN3NR9Okx91hHFcGR1gRbyVNX0qHNqlJC7v_vRmn7K4yMEMOgbxfPngvL4peY3SOMckuvGrAKDgnaZZRyp9EM4xyluQE0afRDCGaJQJx9jx64f0GoRDL6Ul0MvFZdDVvrWlMHUtTxYvb3oH3jTWx1bGMf4Gx_ejjRVe6u8BUvJR9_HU0atia784O0JiX0TMtWw-vpvk0uvm8-DH_kqzWy-v55SpRnKIh4URzrjGWPNU4PAmLTDBaAs1Lgsq0opjnMkVC5kwgyHLGSIqIrihDIgd6Gn3Yndo7-3sEPxRd4xW0rTRgR19wIXKaEnIQUoYRoYIehASHF1F0GGKW0ZwRHuC7_-DGjs6EpITDKEtD8nFAZztUyxaKxmg7OKlqMOBkqAXoJixf8pQShrb60x4dRgVdo_bwj__wIAa4HWo5el9c33w7Vq5_HiuvlkdKsVw9lmf7pLJtCzUU4d_M14_1xU4rZ713oIveNZ10dwVGxbYNiqkNiulfhx1vp0qMZQfVvX-Iv5_i0ivZaieNavw944Fkfyv_Zsc2frDu4VaOiEgJ_QMLDxIV</recordid><startdate>19890303</startdate><enddate>19890303</enddate><creator>Ebihara, L.</creator><creator>Beyer, E. 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Psychology</topic><topic>Gap junctions</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>genes</topic><topic>Genetics</topic><topic>Granulosa cells</topic><topic>Intercellular Junctions - physiology</topic><topic>Junctional complexes (Epithelium)</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - physiology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Oocytes</topic><topic>Oocytes - analysis</topic><topic>Oocytes - physiology</topic><topic>Ovaries</topic><topic>Rats</topic><topic>RNA</topic><topic>RNA - analysis</topic><topic>RNA, Messenger - analysis</topic><topic>Tissue Distribution</topic><topic>Voltage-clamp technique (Electrophysiology)</topic><topic>Xenopus</topic><topic>Xenopus - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebihara, L.</creatorcontrib><creatorcontrib>Beyer, E. 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RNA blot analysis of total Xenopus oocyte RNA showed hybridization to a single 1.6-kilobase band. This messenger RNA is abundant in oocytes, decreases to levels below the sensitivity of our assay by stage 15 (18 hours), and is not detectable in RNA from a number of adult organs. To confirm that the oocyte cDNA encodes a gap junction channel, the protein was over expressed in Xenopus oocytes by injection of RNA synthesized in vitro. Pairs of RNA-injected oocytes formed many more time- and voltage-sensitive cell-cell channels than water-injected pairs.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>2466337</pmid><doi>10.1126/science.2466337</doi><tpages>2</tpages></addata></record> |
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subjects | Amino Acid Sequence Animals Biochemistry Biological and medical sciences Cell Communication Cell junctions Cloning, Molecular Complementary DNA Connexins DNA Probes Electric Conductivity Electric potential Embryos Female Fetuses Fundamental and applied biological sciences. Psychology Gap junctions Gene expression Gene Expression Regulation genes Genetics Granulosa cells Intercellular Junctions - physiology Junctional complexes (Epithelium) Membrane Proteins - genetics Membrane Proteins - physiology Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Hybridization Oocytes Oocytes - analysis Oocytes - physiology Ovaries Rats RNA RNA - analysis RNA, Messenger - analysis Tissue Distribution Voltage-clamp technique (Electrophysiology) Xenopus Xenopus - embryology |
title | Cloning and Expression of a Xenopus Embryonic Gap Junction Protein |
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