Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy
The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorot...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1989-04, Vol.49 (8), p.1937-1940 |
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| container_end_page | 1940 |
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| container_issue | 8 |
| container_start_page | 1937 |
| container_title | Cancer research (Chicago, Ill.) |
| container_volume | 49 |
| creator | LIVESEY, J. C GOLDEN, R. N SHANKLAND, E. G GRUNBAUM, Z RICHARDS, T. L WADE, R. A KROHN, K. A |
| description | The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethylphosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy. |
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Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 2539249</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Amifostine - analogs & derivatives ; Amifostine - metabolism ; Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Disulfides - metabolism ; Fundamental and applied biological sciences. 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G</creatorcontrib><creatorcontrib>GRUNBAUM, Z</creatorcontrib><creatorcontrib>RICHARDS, T. L</creatorcontrib><creatorcontrib>WADE, R. A</creatorcontrib><creatorcontrib>KROHN, K. A</creatorcontrib><title>Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethylphosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.</description><subject>Amifostine - analogs & derivatives</subject><subject>Amifostine - metabolism</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Disulfides - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Oxidation-Reduction</subject><subject>Sulfhydryl Compounds - metabolism</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo67r6E4QcxFuh-WrToyzqCite9Fym6cSNtE1NUnT_vRWLp5nheXhh3hOyZkrorJRSnZJ1nuc6U7Lk5-Qixo_5VCxXK7LiSlRcVmtinxHiFLDHIVFvaXIxTkj9t2shOT9kAdvJ_G40JkhIv1w6UAOhmRkTdJhMhxBoD-8DJmdowOgHGAzSOKJJwUfjx-MlObPQRbxa5oa8Pdy_bnfZ_uXxaXu3zw5Mi5ShBcGbVusGWJUrzU0rsbLMKi3BKlVwUwkh2sIKzplkDaCVmhdcNrwxiGJDbv9yx-A_J4yp7l002HUwoJ9iXWpdCSaKWbxexKnpsa3H4HoIx3opZuY3C4dooLNhfsnFf60odSHmqB-szW8E</recordid><startdate>19890415</startdate><enddate>19890415</enddate><creator>LIVESEY, J. C</creator><creator>GOLDEN, R. N</creator><creator>SHANKLAND, E. 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C</creatorcontrib><creatorcontrib>GOLDEN, R. N</creatorcontrib><creatorcontrib>SHANKLAND, E. G</creatorcontrib><creatorcontrib>GRUNBAUM, Z</creatorcontrib><creatorcontrib>RICHARDS, T. L</creatorcontrib><creatorcontrib>WADE, R. A</creatorcontrib><creatorcontrib>KROHN, K. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIVESEY, J. C</au><au>GOLDEN, R. N</au><au>SHANKLAND, E. G</au><au>GRUNBAUM, Z</au><au>RICHARDS, T. L</au><au>WADE, R. A</au><au>KROHN, K. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1989-04-15</date><risdate>1989</risdate><volume>49</volume><issue>8</issue><spage>1937</spage><epage>1940</epage><pages>1937-1940</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>The oxidation state of tissues influences their response to cancer therapy. We have devised a novel approach to the measurement of thiol redox which is based on the relative nuclear magnetic resonance signal intensity from carbon-13 adjacent to sulfur in metabolites of the redox-sensitive phosphorothioate drug, S-2-(3-methylaminopropylamino)ethylphosphorothioic acid (WR3689). Incubation of WR3689 metabolites under oxidizing conditions results in quantifiable changes in the 13C nuclear magnetic resonance spectrum stoichiometrically related to the degree of oxidation in mouse liver homogenate in vitro. Drug oxidation is competitive with the oxidation of tissue-derived thiol groups under these conditions. Noninvasive measurement of redox state may assist in designing more effective strategies for altering normal and malignant tissue response to cancer therapy.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>2539249</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1989-04, Vol.49 (8), p.1937-1940 |
| issn | 0008-5472 1538-7445 |
| language | eng |
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| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amifostine - analogs & derivatives Amifostine - metabolism Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Disulfides - metabolism Fundamental and applied biological sciences. Psychology Magnetic Resonance Spectroscopy Mice Mice, Inbred C3H Oxidation-Reduction Sulfhydryl Compounds - metabolism |
| title | Measurement of tissue oxidation-reduction state with carbon-13 nuclear magnetic resonance spectroscopy |
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