Adenylate Kinase Activity in Ejaculated Bovine Sperm Flagella
Adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3) activity was detected in the flagella of ejaculated bovine spermatozoa. This activity provided sufficient ATP to produce normal motility in cells permeabilized with digitonin and treated with 0.5 mM MgADP. In the presence of ADP, adenylate ki...
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| Veröffentlicht in: | The Journal of biological chemistry 1989-04, Vol.264 (11), p.6086-6091 |
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| creator | Schoff, P K Cheetham, J Lardy, H A |
| description | Adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3) activity was detected in the flagella of ejaculated bovine spermatozoa. This activity provided sufficient ATP to produce normal motility in cells permeabilized with digitonin and treated with 0.5 mM MgADP. In the presence of ADP, adenylate kinase activity was inhibited by P1, P5-di(adenosine 5′)-pentaphosphate (Ap5A), an adenylate kinase-specific inhibitor, and motility was stopped. ATP-supported motility was not affected by Ap5A. Mitochondrial adenylate kinase activity allowed AMP to stimulate respiration in permeabilized sperm. Adenylate kinase activity in tail fragments was most active in a pH range from 7.6 to 8.4, and a similar pH sensitivity was observed for this enzyme activity in a hypotonic extract of whole sperm. The apparent km of adenylate kinase activity in permeabilized tail fragments was about 1.0 mM ADP in the direction of ATP synthesis. The fluctuation of nucleotide concentrations in normal and metabolically stimulated sperm suggested that adenylate kinase was most active when the cell was highly motile, although adenylate kinase activity did not appear to be coupled strictly with motility. |
| doi_str_mv | 10.1016/S0021-9258(18)83316-6 |
| format | Article |
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This activity provided sufficient ATP to produce normal motility in cells permeabilized with digitonin and treated with 0.5 mM MgADP. In the presence of ADP, adenylate kinase activity was inhibited by P1, P5-di(adenosine 5′)-pentaphosphate (Ap5A), an adenylate kinase-specific inhibitor, and motility was stopped. ATP-supported motility was not affected by Ap5A. Mitochondrial adenylate kinase activity allowed AMP to stimulate respiration in permeabilized sperm. Adenylate kinase activity in tail fragments was most active in a pH range from 7.6 to 8.4, and a similar pH sensitivity was observed for this enzyme activity in a hypotonic extract of whole sperm. The apparent km of adenylate kinase activity in permeabilized tail fragments was about 1.0 mM ADP in the direction of ATP synthesis. The fluctuation of nucleotide concentrations in normal and metabolically stimulated sperm suggested that adenylate kinase was most active when the cell was highly motile, although adenylate kinase activity did not appear to be coupled strictly with motility.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)83316-6</identifier><identifier>PMID: 2539368</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Adenosine Diphosphate - metabolism ; Adenosine Triphosphate - metabolism ; Adenylate Kinase - metabolism ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; BOVIN ; Caffeine - pharmacology ; CATTLE ; EJACULATION ; Energy Metabolism ; Enzymes and enzyme inhibitors ; ENZYMIC ACTIVITY ; ESPERMATOZOO ; EYACULACION ; Fluorides - pharmacology ; Fundamental and applied biological sciences. Psychology ; GANADO BOVINO ; Hydrogen-Ion Concentration ; In Vitro Techniques ; Kinetics ; Male ; Oligomycins - pharmacology ; Phosphotransferases - metabolism ; Sperm Motility ; Sperm Tail - enzymology ; spermatocytes ; SPERMATOZOA ; Spermatozoa - enzymology ; SPERMATOZOIDE ; TRANSFERASAS ; TRANSFERASE ; TRANSFERASES</subject><ispartof>The Journal of biological chemistry, 1989-04, Vol.264 (11), p.6086-6091</ispartof><rights>1989 © 1989 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-1877b41fbeb6f0b3a8354fe9c51a94e09db083a7c8677eaf206738dde59c79b73</citedby><cites>FETCH-LOGICAL-c566t-1877b41fbeb6f0b3a8354fe9c51a94e09db083a7c8677eaf206738dde59c79b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7310580$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2539368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schoff, P K</creatorcontrib><creatorcontrib>Cheetham, J</creatorcontrib><creatorcontrib>Lardy, H A</creatorcontrib><title>Adenylate Kinase Activity in Ejaculated Bovine Sperm Flagella</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3) activity was detected in the flagella of ejaculated bovine spermatozoa. This activity provided sufficient ATP to produce normal motility in cells permeabilized with digitonin and treated with 0.5 mM MgADP. In the presence of ADP, adenylate kinase activity was inhibited by P1, P5-di(adenosine 5′)-pentaphosphate (Ap5A), an adenylate kinase-specific inhibitor, and motility was stopped. ATP-supported motility was not affected by Ap5A. Mitochondrial adenylate kinase activity allowed AMP to stimulate respiration in permeabilized sperm. Adenylate kinase activity in tail fragments was most active in a pH range from 7.6 to 8.4, and a similar pH sensitivity was observed for this enzyme activity in a hypotonic extract of whole sperm. The apparent km of adenylate kinase activity in permeabilized tail fragments was about 1.0 mM ADP in the direction of ATP synthesis. The fluctuation of nucleotide concentrations in normal and metabolically stimulated sperm suggested that adenylate kinase was most active when the cell was highly motile, although adenylate kinase activity did not appear to be coupled strictly with motility.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Adenylate Kinase - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BOVIN</subject><subject>Caffeine - pharmacology</subject><subject>CATTLE</subject><subject>EJACULATION</subject><subject>Energy Metabolism</subject><subject>Enzymes and enzyme inhibitors</subject><subject>ENZYMIC ACTIVITY</subject><subject>ESPERMATOZOO</subject><subject>EYACULACION</subject><subject>Fluorides - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GANADO BOVINO</subject><subject>Hydrogen-Ion Concentration</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Male</subject><subject>Oligomycins - pharmacology</subject><subject>Phosphotransferases - metabolism</subject><subject>Sperm Motility</subject><subject>Sperm Tail - enzymology</subject><subject>spermatocytes</subject><subject>SPERMATOZOA</subject><subject>Spermatozoa - enzymology</subject><subject>SPERMATOZOIDE</subject><subject>TRANSFERASAS</subject><subject>TRANSFERASE</subject><subject>TRANSFERASES</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1rFDEYh4Moda3-A0JhQBE9jOadTL4OItvSqljwsBa8hUzmnd2U-ViT2S3735vpLHttLjn8nvfrIeQC6GegIL6sKC0g1wVXH0F9UoyByMUzsgCqWM44_H1OFifkJXkV4z1Nr9RwRs4KzjQTakG-LmvsD60dMfvlexsxW7rR7_14yHyfXd9bt5vCOrsc9r7HbLXF0GU3rV1j29rX5EVj24hvjv85ubu5_nP1I7_9_f3n1fI2d1yIMQclZVVCU2ElGloxqxgvG9SOg9UlUl1XaWkrnRJSom0KKiRTdY1cO6kryc7Jh7nvNgz_dhhH0_nopg16HHbRSKV0QSV7EgTOOC9VkUA-gy4MMQZszDb4zoaDAWomv-bRr5nkGVDm0a8Rqe7iOGBXdVifqo5CU_7-mNvobNsE2zsfT5hkQLmiCXs3Yxu_3jz4gKbyg9tgZwpRGgAjqJqGvZ2pxg7GrkNqdLdSGgTTOoXf5hCT-L3HYKLz2DusUzs3mnrwT9zyH20DqT0</recordid><startdate>19890415</startdate><enddate>19890415</enddate><creator>Schoff, P K</creator><creator>Cheetham, J</creator><creator>Lardy, H A</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19890415</creationdate><title>Adenylate Kinase Activity in Ejaculated Bovine Sperm Flagella</title><author>Schoff, P K ; Cheetham, J ; Lardy, H A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-1877b41fbeb6f0b3a8354fe9c51a94e09db083a7c8677eaf206738dde59c79b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Adenylate Kinase - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BOVIN</topic><topic>Caffeine - pharmacology</topic><topic>CATTLE</topic><topic>EJACULATION</topic><topic>Energy Metabolism</topic><topic>Enzymes and enzyme inhibitors</topic><topic>ENZYMIC ACTIVITY</topic><topic>ESPERMATOZOO</topic><topic>EYACULACION</topic><topic>Fluorides - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GANADO BOVINO</topic><topic>Hydrogen-Ion Concentration</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Male</topic><topic>Oligomycins - pharmacology</topic><topic>Phosphotransferases - metabolism</topic><topic>Sperm Motility</topic><topic>Sperm Tail - enzymology</topic><topic>spermatocytes</topic><topic>SPERMATOZOA</topic><topic>Spermatozoa - enzymology</topic><topic>SPERMATOZOIDE</topic><topic>TRANSFERASAS</topic><topic>TRANSFERASE</topic><topic>TRANSFERASES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schoff, P K</creatorcontrib><creatorcontrib>Cheetham, J</creatorcontrib><creatorcontrib>Lardy, H A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schoff, P K</au><au>Cheetham, J</au><au>Lardy, H A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adenylate Kinase Activity in Ejaculated Bovine Sperm Flagella</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1989-04-15</date><risdate>1989</risdate><volume>264</volume><issue>11</issue><spage>6086</spage><epage>6091</epage><pages>6086-6091</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Adenylate kinase (ATP:AMP phosphotransferase, EC 2.7.4.3) activity was detected in the flagella of ejaculated bovine spermatozoa. This activity provided sufficient ATP to produce normal motility in cells permeabilized with digitonin and treated with 0.5 mM MgADP. In the presence of ADP, adenylate kinase activity was inhibited by P1, P5-di(adenosine 5′)-pentaphosphate (Ap5A), an adenylate kinase-specific inhibitor, and motility was stopped. ATP-supported motility was not affected by Ap5A. Mitochondrial adenylate kinase activity allowed AMP to stimulate respiration in permeabilized sperm. Adenylate kinase activity in tail fragments was most active in a pH range from 7.6 to 8.4, and a similar pH sensitivity was observed for this enzyme activity in a hypotonic extract of whole sperm. The apparent km of adenylate kinase activity in permeabilized tail fragments was about 1.0 mM ADP in the direction of ATP synthesis. The fluctuation of nucleotide concentrations in normal and metabolically stimulated sperm suggested that adenylate kinase was most active when the cell was highly motile, although adenylate kinase activity did not appear to be coupled strictly with motility.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2539368</pmid><doi>10.1016/S0021-9258(18)83316-6</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1989-04, Vol.264 (11), p.6086-6091 |
| issn | 0021-9258 1083-351X |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Adenylate Kinase - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences BOVIN Caffeine - pharmacology CATTLE EJACULATION Energy Metabolism Enzymes and enzyme inhibitors ENZYMIC ACTIVITY ESPERMATOZOO EYACULACION Fluorides - pharmacology Fundamental and applied biological sciences. Psychology GANADO BOVINO Hydrogen-Ion Concentration In Vitro Techniques Kinetics Male Oligomycins - pharmacology Phosphotransferases - metabolism Sperm Motility Sperm Tail - enzymology spermatocytes SPERMATOZOA Spermatozoa - enzymology SPERMATOZOIDE TRANSFERASAS TRANSFERASE TRANSFERASES |
| title | Adenylate Kinase Activity in Ejaculated Bovine Sperm Flagella |
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