Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media
We described here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are de...
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Veröffentlicht in: | Biosensors & bioelectronics 1997, Vol.12 (3), p.205-212 |
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creator | Bedioui, Fethi Trevin, Stéphane Dvynck, Jacques Lantoine, Frédérique Brunet, Annie Devynck, Marie-Aude |
description | We described here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein. |
doi_str_mv | 10.1016/S0956-5663(97)85338-9 |
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In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(97)85338-9</identifier><identifier>PMID: 9115688</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques ; Biotechnology ; blood platelets ; Blood Platelets - metabolism ; Cells, Cultured ; Culture Media - chemistry ; electrochemical detection ; Electrochemistry - instrumentation ; Electrochemistry - methods ; endothelial cells ; Endothelium, Vascular - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Methods. Procedures. 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In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biotechnology</subject><subject>blood platelets</subject><subject>Blood Platelets - metabolism</subject><subject>Cells, Cultured</subject><subject>Culture Media - chemistry</subject><subject>electrochemical detection</subject><subject>Electrochemistry - instrumentation</subject><subject>Electrochemistry - methods</subject><subject>endothelial cells</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Methods. Procedures. Technologies</subject><subject>Nickel</subject><subject>nitric oxide</subject><subject>Nitric Oxide - analysis</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Others</subject><subject>porphyrinic sensor</subject><subject>ultramicroelectrodes</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P1SAUxYnRjM_RjzAJC2N0UYVHobAyk8n4J5nEhbomt3DroLQ8oTUzH8TvK2_avK0bWNzfOXDOJeSCs7eccfXuKzNSNVIp8dp0b7QUQjfmEdlx3Ymm3Qv5mOxOyFPyrJSfjLGOG3ZGzgznUmm9I3-vI_QpwxzSRGHydClI00Cn4H5hpIcIE2Q6gsvJ3bsYHHVpPES8a3oo6GnBqaRc6JAynW-R-pDRzRRjPavkFsfgINIRoSwZR5zm1X3O1SrdBY80TLQPKaYfG-kDPCdPBogFX2z3Ofn-4frb1afm5svHz1eXN41rpZwb55gHo6RRneC8BTAwSOe1ER2DXnR-YBx6V4O2KLT2Xa9bNyhAzfW-ZyjOyavV95DT7wXLbMdQHMaaGtNSbKe14aI1FZQrWHsoJeNgDzmMkO8tZ_a4DvuwDnvs2prOPqzDHnUX2wNLX5OdVFv_df5ym0Op6YcMkwvlhO2lMkyqir1fMaxl_AmYbXEBJ4dr3dan8J-P_AMhq6rD</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Bedioui, Fethi</creator><creator>Trevin, Stéphane</creator><creator>Dvynck, Jacques</creator><creator>Lantoine, Frédérique</creator><creator>Brunet, Annie</creator><creator>Devynck, Marie-Aude</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media</title><author>Bedioui, Fethi ; Trevin, Stéphane ; Dvynck, Jacques ; Lantoine, Frédérique ; Brunet, Annie ; Devynck, Marie-Aude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-cc0da9659673114aa9af5cd89370ab37df01abc6884e388d7b84cf6ae8182b0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biotechnology</topic><topic>blood platelets</topic><topic>Blood Platelets - metabolism</topic><topic>Cells, Cultured</topic><topic>Culture Media - chemistry</topic><topic>electrochemical detection</topic><topic>Electrochemistry - instrumentation</topic><topic>Electrochemistry - methods</topic><topic>endothelial cells</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Methods. Procedures. 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subjects | Biological and medical sciences Biosensing Techniques Biotechnology blood platelets Blood Platelets - metabolism Cells, Cultured Culture Media - chemistry electrochemical detection Electrochemistry - instrumentation Electrochemistry - methods endothelial cells Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Methods. Procedures. Technologies Nickel nitric oxide Nitric Oxide - analysis Nitric Oxide - biosynthesis Others porphyrinic sensor ultramicroelectrodes Various methods and equipments |
title | Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media |
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