Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media

We described here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are de...

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Veröffentlicht in:Biosensors & bioelectronics 1997, Vol.12 (3), p.205-212
Hauptverfasser: Bedioui, Fethi, Trevin, Stéphane, Dvynck, Jacques, Lantoine, Frédérique, Brunet, Annie, Devynck, Marie-Aude
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container_end_page 212
container_issue 3
container_start_page 205
container_title Biosensors & bioelectronics
container_volume 12
creator Bedioui, Fethi
Trevin, Stéphane
Dvynck, Jacques
Lantoine, Frédérique
Brunet, Annie
Devynck, Marie-Aude
description We described here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein.
doi_str_mv 10.1016/S0956-5663(97)85338-9
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Biological and medical sciences
Biosensing Techniques
Biotechnology
blood platelets
Blood Platelets - metabolism
Cells, Cultured
Culture Media - chemistry
electrochemical detection
Electrochemistry - instrumentation
Electrochemistry - methods
endothelial cells
Endothelium, Vascular - metabolism
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Methods. Procedures. Technologies
Nickel
nitric oxide
Nitric Oxide - analysis
Nitric Oxide - biosynthesis
Others
porphyrinic sensor
ultramicroelectrodes
Various methods and equipments
title Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media
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