Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens
Partially purified low molecular weight antigens obtained by gel filtration of whole worm homogenates or total adult excretory–secretory (ES) products were tested in a vaccination experiment to determine their ability to induce protective immunity against Haemonchus contortus in sheep. Sheep were ch...
Gespeichert in:
| Veröffentlicht in: | Parasitology 1997-03, Vol.114 (3), p.293-299 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 299 |
|---|---|
| container_issue | 3 |
| container_start_page | 293 |
| container_title | Parasitology |
| container_volume | 114 |
| creator | SCHALLIG, H. D. F. H. VAN LEEUWEN, M. A. W. |
| description | Partially purified low molecular weight antigens obtained by
gel filtration of whole worm homogenates or total adult
excretory–secretory (ES) products were tested in a vaccination
experiment to determine their ability to induce protective
immunity against Haemonchus contortus in sheep. Sheep were challenged
with 20000 infective 3rd-stage larvae. One animal
in the low molecular weight vaccinated group showed no protection
against H. contortus, whereas the 4 other sheep in this
group showed a mean reduction of 99·9% in faecal egg counts
and of 97·6% in abomasal worm burden compared to the
non-vaccinated controls and the adjuvant controls. The ES-vaccinated
sheep showed a 32·2% reduction in parasite egg
production and a 63·7% reduction in abomasal worm counts.
Analysis of the humoral immune responses revealed no
significant differences in antibody recognition of putative protective
antigens between the protected and non-protected
vaccinated animals. However, a marked lower lymphocyte proliferation
response was found in non-protected sheep. |
| doi_str_mv | 10.1017/S0031182096008517 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78888943</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0031182096008517</cupid><sourcerecordid>78888943</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-a9f66988477338615ca542da4ca0248f85e12539f195ed3bb7e73563205eb7dd3</originalsourceid><addsrcrecordid>eNqFkc9uEzEQxi0EKmnhATgg-YC4Ldjr9Z89QkVbRARUwNny2rOJy64dbG9DHqFvzUaJckGCuXik7zczn_wh9IKSN5RQ-fYbIYxSVZNWEKI4lY_QgjairRQV9DFa7OVqrz9F5znfEUIEE_UZOmuJ5KxRC_TwNcUCtvh7wH4cp-DLDpeIyxpwN8Toqh7A-bDCAUZTogN8Y2CMwa6njG0MJaYydz64yYLD3Q7fG2t9MMXHgLe-rPHGJJN9ATzELR7jAHYaTMJb8Kt1wSYUv4KQn6EnvRkyPD--F-jH1YfvlzfV8sv1x8t3y8o2TJXKtL0QrVKNlIwpQbk1vKmdaawhdaN6xYHWnLU9bTk41nUSJOOC1YRDJ51jF-j1Ye8mxV8T5KJHny0MgwkQp6ylmqtt2H9BKmrGCKUzSA-gTTHnBL3eJD-atNOU6H1O-q-c5pmXx-VTN4I7TRyDmfVXR91ka4Y-mWB9PmG1ILPF_enqgPlc4PdJNumnFpJJrsX1rV5-vl2y-v2V_jTz7GjVjF3ybgX6Lk4pzB_-D7N_AOSxurk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16233011</pqid></control><display><type>article</type><title>Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens</title><source>MEDLINE</source><source>Cambridge University Press Journals Complete</source><creator>SCHALLIG, H. D. F. H. ; VAN LEEUWEN, M. A. W.</creator><creatorcontrib>SCHALLIG, H. D. F. H. ; VAN LEEUWEN, M. A. W.</creatorcontrib><description>Partially purified low molecular weight antigens obtained by
gel filtration of whole worm homogenates or total adult
excretory–secretory (ES) products were tested in a vaccination
experiment to determine their ability to induce protective
immunity against Haemonchus contortus in sheep. Sheep were challenged
with 20000 infective 3rd-stage larvae. One animal
in the low molecular weight vaccinated group showed no protection
against H. contortus, whereas the 4 other sheep in this
group showed a mean reduction of 99·9% in faecal egg counts
and of 97·6% in abomasal worm burden compared to the
non-vaccinated controls and the adjuvant controls. The ES-vaccinated
sheep showed a 32·2% reduction in parasite egg
production and a 63·7% reduction in abomasal worm counts.
Analysis of the humoral immune responses revealed no
significant differences in antibody recognition of putative protective
antigens between the protected and non-protected
vaccinated animals. However, a marked lower lymphocyte proliferation
response was found in non-protected sheep.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182096008517</identifier><identifier>PMID: 9075348</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Animal productions ; Animals ; Antibodies, Helminth - blood ; Antigens, Helminth - immunology ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Haemonchiasis - immunology ; Haemonchiasis - parasitology ; Haemonchiasis - prevention & control ; Haemonchiasis - veterinary ; Haemonchus - immunology ; Haemonchus - physiology ; Haemonchus contortus ; low molecular weight antigens ; Lymphocyte Activation ; Molecular Weight ; nematodes ; Parasite Egg Count - veterinary ; Sheep ; Sheep Diseases - immunology ; Sheep Diseases - parasitology ; Sheep Diseases - prevention & control ; Terrestrial animal productions ; vaccination ; Vaccination - veterinary ; Vaccines - immunology ; Vertebrates</subject><ispartof>Parasitology, 1997-03, Vol.114 (3), p.293-299</ispartof><rights>1997 Cambridge University Press</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-a9f66988477338615ca542da4ca0248f85e12539f195ed3bb7e73563205eb7dd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182096008517/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2609431$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9075348$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHALLIG, H. D. F. H.</creatorcontrib><creatorcontrib>VAN LEEUWEN, M. A. W.</creatorcontrib><title>Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Partially purified low molecular weight antigens obtained by
gel filtration of whole worm homogenates or total adult
excretory–secretory (ES) products were tested in a vaccination
experiment to determine their ability to induce protective
immunity against Haemonchus contortus in sheep. Sheep were challenged
with 20000 infective 3rd-stage larvae. One animal
in the low molecular weight vaccinated group showed no protection
against H. contortus, whereas the 4 other sheep in this
group showed a mean reduction of 99·9% in faecal egg counts
and of 97·6% in abomasal worm burden compared to the
non-vaccinated controls and the adjuvant controls. The ES-vaccinated
sheep showed a 32·2% reduction in parasite egg
production and a 63·7% reduction in abomasal worm counts.
Analysis of the humoral immune responses revealed no
significant differences in antibody recognition of putative protective
antigens between the protected and non-protected
vaccinated animals. However, a marked lower lymphocyte proliferation
response was found in non-protected sheep.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens, Helminth - immunology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haemonchiasis - immunology</subject><subject>Haemonchiasis - parasitology</subject><subject>Haemonchiasis - prevention & control</subject><subject>Haemonchiasis - veterinary</subject><subject>Haemonchus - immunology</subject><subject>Haemonchus - physiology</subject><subject>Haemonchus contortus</subject><subject>low molecular weight antigens</subject><subject>Lymphocyte Activation</subject><subject>Molecular Weight</subject><subject>nematodes</subject><subject>Parasite Egg Count - veterinary</subject><subject>Sheep</subject><subject>Sheep Diseases - immunology</subject><subject>Sheep Diseases - parasitology</subject><subject>Sheep Diseases - prevention & control</subject><subject>Terrestrial animal productions</subject><subject>vaccination</subject><subject>Vaccination - veterinary</subject><subject>Vaccines - immunology</subject><subject>Vertebrates</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9uEzEQxi0EKmnhATgg-YC4Ldjr9Z89QkVbRARUwNny2rOJy64dbG9DHqFvzUaJckGCuXik7zczn_wh9IKSN5RQ-fYbIYxSVZNWEKI4lY_QgjairRQV9DFa7OVqrz9F5znfEUIEE_UZOmuJ5KxRC_TwNcUCtvh7wH4cp-DLDpeIyxpwN8Toqh7A-bDCAUZTogN8Y2CMwa6njG0MJaYydz64yYLD3Q7fG2t9MMXHgLe-rPHGJJN9ATzELR7jAHYaTMJb8Kt1wSYUv4KQn6EnvRkyPD--F-jH1YfvlzfV8sv1x8t3y8o2TJXKtL0QrVKNlIwpQbk1vKmdaawhdaN6xYHWnLU9bTk41nUSJOOC1YRDJ51jF-j1Ye8mxV8T5KJHny0MgwkQp6ylmqtt2H9BKmrGCKUzSA-gTTHnBL3eJD-atNOU6H1O-q-c5pmXx-VTN4I7TRyDmfVXR91ka4Y-mWB9PmG1ILPF_enqgPlc4PdJNumnFpJJrsX1rV5-vl2y-v2V_jTz7GjVjF3ybgX6Lk4pzB_-D7N_AOSxurk</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>SCHALLIG, H. D. F. H.</creator><creator>VAN LEEUWEN, M. A. W.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970301</creationdate><title>Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens</title><author>SCHALLIG, H. D. F. H. ; VAN LEEUWEN, M. A. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-a9f66988477338615ca542da4ca0248f85e12539f195ed3bb7e73563205eb7dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigens, Helminth - immunology</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Haemonchiasis - immunology</topic><topic>Haemonchiasis - parasitology</topic><topic>Haemonchiasis - prevention & control</topic><topic>Haemonchiasis - veterinary</topic><topic>Haemonchus - immunology</topic><topic>Haemonchus - physiology</topic><topic>Haemonchus contortus</topic><topic>low molecular weight antigens</topic><topic>Lymphocyte Activation</topic><topic>Molecular Weight</topic><topic>nematodes</topic><topic>Parasite Egg Count - veterinary</topic><topic>Sheep</topic><topic>Sheep Diseases - immunology</topic><topic>Sheep Diseases - parasitology</topic><topic>Sheep Diseases - prevention & control</topic><topic>Terrestrial animal productions</topic><topic>vaccination</topic><topic>Vaccination - veterinary</topic><topic>Vaccines - immunology</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SCHALLIG, H. D. F. H.</creatorcontrib><creatorcontrib>VAN LEEUWEN, M. A. W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SCHALLIG, H. D. F. H.</au><au>VAN LEEUWEN, M. A. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>114</volume><issue>3</issue><spage>293</spage><epage>299</epage><pages>293-299</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Partially purified low molecular weight antigens obtained by
gel filtration of whole worm homogenates or total adult
excretory–secretory (ES) products were tested in a vaccination
experiment to determine their ability to induce protective
immunity against Haemonchus contortus in sheep. Sheep were challenged
with 20000 infective 3rd-stage larvae. One animal
in the low molecular weight vaccinated group showed no protection
against H. contortus, whereas the 4 other sheep in this
group showed a mean reduction of 99·9% in faecal egg counts
and of 97·6% in abomasal worm burden compared to the
non-vaccinated controls and the adjuvant controls. The ES-vaccinated
sheep showed a 32·2% reduction in parasite egg
production and a 63·7% reduction in abomasal worm counts.
Analysis of the humoral immune responses revealed no
significant differences in antibody recognition of putative protective
antigens between the protected and non-protected
vaccinated animals. However, a marked lower lymphocyte proliferation
response was found in non-protected sheep.</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><pmid>9075348</pmid><doi>10.1017/S0031182096008517</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-1820 |
| ispartof | Parasitology, 1997-03, Vol.114 (3), p.293-299 |
| issn | 0031-1820 1469-8161 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78888943 |
| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animal productions Animals Antibodies, Helminth - blood Antigens, Helminth - immunology Biological and medical sciences Fundamental and applied biological sciences. Psychology Haemonchiasis - immunology Haemonchiasis - parasitology Haemonchiasis - prevention & control Haemonchiasis - veterinary Haemonchus - immunology Haemonchus - physiology Haemonchus contortus low molecular weight antigens Lymphocyte Activation Molecular Weight nematodes Parasite Egg Count - veterinary Sheep Sheep Diseases - immunology Sheep Diseases - parasitology Sheep Diseases - prevention & control Terrestrial animal productions vaccination Vaccination - veterinary Vaccines - immunology Vertebrates |
| title | Protective immunity to the blood-feeding nematode Haemonchus contortus induced by vaccination with parasite low molecular weight antigens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T01%3A18%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20immunity%20to%20the%20blood-feeding%20nematode%20Haemonchus%20contortus%20induced%20by%20vaccination%20with%20parasite%20low%20molecular%20weight%20antigens&rft.jtitle=Parasitology&rft.au=SCHALLIG,%20H.%20D.%20F.%20H.&rft.date=1997-03-01&rft.volume=114&rft.issue=3&rft.spage=293&rft.epage=299&rft.pages=293-299&rft.issn=0031-1820&rft.eissn=1469-8161&rft.coden=PARAAE&rft_id=info:doi/10.1017/S0031182096008517&rft_dat=%3Cproquest_cross%3E78888943%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16233011&rft_id=info:pmid/9075348&rft_cupid=10_1017_S0031182096008517&rfr_iscdi=true |