Restriction fragment length patterns of dna from parasitic nematodes of sheep
High molecular weight DNA obtained from sheep parasitic nematodes Haemonchus contortus, Ostertagia circumcincta and Trichostrongylus colubriformis, was digested with various restriction endonucleases. Digestion with Eco R1 produced the most informative pattern of repeat sequence bands. H contortus a...
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| Veröffentlicht in: | Research in veterinary science 1989-01, Vol.46 (1), p.127-128 |
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| container_title | Research in veterinary science |
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| creator | BEH, K.J. FOLEY, R.C. GOODWIN, E.J. |
| description | High molecular weight DNA obtained from sheep parasitic nematodes Haemonchus contortus, Ostertagia circumcincta and Trichostrongylus colubriformis, was digested with various restriction endonucleases. Digestion with Eco R1 produced the most informative pattern of repeat sequence bands. H contortus adult or larval dna produced bands of 2·7, 3·0 and 1·4 kb. O circumcincta adult or larval dna had common 2·7 and 1·4 kb bands with adult specific bands of 2·2 and 0·9 kb and a larval specific 2·08 kb band. T colubriformis adults or larval dna produced 2·7, 1·4 and 0·79 kb bands. These preliminary results show that restriction patterns of repeal sequence dna may be useful for the identification of various trichostrongylid species parasitic for sheep. |
| doi_str_mv | 10.1016/S0034-5288(18)31132-9 |
| format | Article |
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Digestion with Eco R1 produced the most informative pattern of repeat sequence bands. H contortus adult or larval dna produced bands of 2·7, 3·0 and 1·4 kb. O circumcincta adult or larval dna had common 2·7 and 1·4 kb bands with adult specific bands of 2·2 and 0·9 kb and a larval specific 2·08 kb band. T colubriformis adults or larval dna produced 2·7, 1·4 and 0·79 kb bands. These preliminary results show that restriction patterns of repeal sequence dna may be useful for the identification of various trichostrongylid species parasitic for sheep.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/S0034-5288(18)31132-9</identifier><identifier>PMID: 2564211</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adults ; Animals ; Deoxyribonucleic acid ; DNA ; DNA - analysis ; DNA - genetics ; DNA Restriction Enzymes - metabolism ; genetic techniques and protocols ; Haemonchus - classification ; Haemonchus - genetics ; Haemonchus contortus ; Molecular chains ; Molecular weight ; Nematodes ; Nucleotide sequence ; Ostertagia - classification ; Ostertagia - genetics ; Ovis aries ; Polymorphism, Restriction Fragment Length ; Repetitive Sequences, Nucleic Acid ; Sheep ; Teladorsagia circumcincta ; Trichostrongyloidea - classification ; Trichostrongyloidea - genetics ; Trichostrongylus - classification ; Trichostrongylus - genetics ; Trichostrongylus colubriformis ; Veterinary medicine</subject><ispartof>Research in veterinary science, 1989-01, Vol.46 (1), p.127-128</ispartof><rights>1989</rights><rights>Copyright Elsevier Limited Jan 1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-d59c48a2d8aab6ef80e0f9541249ead58ca75e04a13444e83abf36332a7fcfb23</citedby><cites>FETCH-LOGICAL-c412t-d59c48a2d8aab6ef80e0f9541249ead58ca75e04a13444e83abf36332a7fcfb23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0034528818311329$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2564211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BEH, K.J.</creatorcontrib><creatorcontrib>FOLEY, R.C.</creatorcontrib><creatorcontrib>GOODWIN, E.J.</creatorcontrib><title>Restriction fragment length patterns of dna from parasitic nematodes of sheep</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>High molecular weight DNA obtained from sheep parasitic nematodes Haemonchus contortus, Ostertagia circumcincta and Trichostrongylus colubriformis, was digested with various restriction endonucleases. Digestion with Eco R1 produced the most informative pattern of repeat sequence bands. H contortus adult or larval dna produced bands of 2·7, 3·0 and 1·4 kb. O circumcincta adult or larval dna had common 2·7 and 1·4 kb bands with adult specific bands of 2·2 and 0·9 kb and a larval specific 2·08 kb band. T colubriformis adults or larval dna produced 2·7, 1·4 and 0·79 kb bands. These preliminary results show that restriction patterns of repeal sequence dna may be useful for the identification of various trichostrongylid species parasitic for sheep.</description><subject>Adults</subject><subject>Animals</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - genetics</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>genetic techniques and protocols</subject><subject>Haemonchus - classification</subject><subject>Haemonchus - genetics</subject><subject>Haemonchus contortus</subject><subject>Molecular chains</subject><subject>Molecular weight</subject><subject>Nematodes</subject><subject>Nucleotide sequence</subject><subject>Ostertagia - classification</subject><subject>Ostertagia - genetics</subject><subject>Ovis aries</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Sheep</subject><subject>Teladorsagia circumcincta</subject><subject>Trichostrongyloidea - classification</subject><subject>Trichostrongyloidea - genetics</subject><subject>Trichostrongylus - classification</subject><subject>Trichostrongylus - genetics</subject><subject>Trichostrongylus colubriformis</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEQhkVpSJ20PyF0oVDSw6YafexqT6GEfkFCoGnOYqwdOQrelSvJhf77KrbJoZeeRsz7zDB6GDsDfgEcuo93nEvVamHMOZgPEkCKdnjBFqDrQ3QdvGSLZ-QVO8n5kXOuAPpjdix0pwTAgt38oFxScCXEufEJVxPNpVnTvCoPzQZLoTTnJvpmnLHmcarNhDmU4JqZJixxpF2eH4g2r9mRx3WmN4d6yu6_fP559a29vv36_erTdesUiNKOenDKoBgN4rIjbzhxP-iaqYFw1MZhr4krBKmUIiNx6WUnpcDeO78U8pS93-_dpPhrW39gp5Adrdc4U9xm2xtjdM91Bd_9Az7GbZrrbVZwrQcO1U6l9J5yKeacyNtNChOmPxa4fZJtd7Ltk0kLxu5k26HOnR22b5cTjc9TB7s1f7vPPUaLqxSyvb8THCQX3SC0lpW43BNUbf0OlGx2gWZHY0jkih1j-M8NfwGRMJdp</recordid><startdate>198901</startdate><enddate>198901</enddate><creator>BEH, K.J.</creator><creator>FOLEY, R.C.</creator><creator>GOODWIN, E.J.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198901</creationdate><title>Restriction fragment length patterns of dna from parasitic nematodes of sheep</title><author>BEH, K.J. ; FOLEY, R.C. ; GOODWIN, E.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-d59c48a2d8aab6ef80e0f9541249ead58ca75e04a13444e83abf36332a7fcfb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adults</topic><topic>Animals</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA - genetics</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>genetic techniques and protocols</topic><topic>Haemonchus - classification</topic><topic>Haemonchus - genetics</topic><topic>Haemonchus contortus</topic><topic>Molecular chains</topic><topic>Molecular weight</topic><topic>Nematodes</topic><topic>Nucleotide sequence</topic><topic>Ostertagia - classification</topic><topic>Ostertagia - genetics</topic><topic>Ovis aries</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Sheep</topic><topic>Teladorsagia circumcincta</topic><topic>Trichostrongyloidea - classification</topic><topic>Trichostrongyloidea - genetics</topic><topic>Trichostrongylus - classification</topic><topic>Trichostrongylus - genetics</topic><topic>Trichostrongylus colubriformis</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BEH, K.J.</creatorcontrib><creatorcontrib>FOLEY, R.C.</creatorcontrib><creatorcontrib>GOODWIN, E.J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BEH, K.J.</au><au>FOLEY, R.C.</au><au>GOODWIN, E.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restriction fragment length patterns of dna from parasitic nematodes of sheep</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>1989-01</date><risdate>1989</risdate><volume>46</volume><issue>1</issue><spage>127</spage><epage>128</epage><pages>127-128</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>High molecular weight DNA obtained from sheep parasitic nematodes Haemonchus contortus, Ostertagia circumcincta and Trichostrongylus colubriformis, was digested with various restriction endonucleases. Digestion with Eco R1 produced the most informative pattern of repeat sequence bands. H contortus adult or larval dna produced bands of 2·7, 3·0 and 1·4 kb. O circumcincta adult or larval dna had common 2·7 and 1·4 kb bands with adult specific bands of 2·2 and 0·9 kb and a larval specific 2·08 kb band. T colubriformis adults or larval dna produced 2·7, 1·4 and 0·79 kb bands. These preliminary results show that restriction patterns of repeal sequence dna may be useful for the identification of various trichostrongylid species parasitic for sheep.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>2564211</pmid><doi>10.1016/S0034-5288(18)31132-9</doi><tpages>2</tpages></addata></record> |
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| subjects | Adults Animals Deoxyribonucleic acid DNA DNA - analysis DNA - genetics DNA Restriction Enzymes - metabolism genetic techniques and protocols Haemonchus - classification Haemonchus - genetics Haemonchus contortus Molecular chains Molecular weight Nematodes Nucleotide sequence Ostertagia - classification Ostertagia - genetics Ovis aries Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Sheep Teladorsagia circumcincta Trichostrongyloidea - classification Trichostrongyloidea - genetics Trichostrongylus - classification Trichostrongylus - genetics Trichostrongylus colubriformis Veterinary medicine |
| title | Restriction fragment length patterns of dna from parasitic nematodes of sheep |
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