Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A
Wnt genes encode secreted proteins which are implicated in receptor-mediated cell-cell signaling events important in embryogenesis, but the second messenger systems modulated by Wnts have not been identified. We report that ectopic expression of Xwnt-5A in zebrafish embryos enhances the frequency of...
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| Veröffentlicht in: | Developmental biology 1997-02, Vol.182 (1), p.114-120 |
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| container_title | Developmental biology |
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| creator | Slusarski, D C Yang-Snyder, J Busa, W B Moon, R T |
| description | Wnt genes encode secreted proteins which are implicated in receptor-mediated cell-cell signaling events important in embryogenesis, but the second messenger systems modulated by Wnts have not been identified. We report that ectopic expression of Xwnt-5A in zebrafish embryos enhances the frequency of intracellular Ca2+ transients in the enveloping layer of the blastodisc, whereas Xwnt-8 does not. These transients are independent of extracellular Ca2+. Consistent with the observed Ca2+ transients playing a role in responses of embryos to Xwnt-5A, we report that the ligand-activated serotonin type 1C receptor, which stimulates PI cycle activity and Ca2+ signaling independent of Wnts, phenocopies embryonic responses to Xwnt-5A. These results suggest that intercellular signaling by a subset of vertebrate Wnts involves modulation of a intracellular Ca2+ signaling pathway, which may arise from phosphatidylinositol cycle activity. |
| doi_str_mv | 10.1006/dbio.1996.8463 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 1997-02, Vol.182 (1), p.114-120 |
| issn | 0012-1606 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Blastocyst - physiology Calcium - metabolism Embryo, Nonmammalian - physiology Proto-Oncogene Proteins - biosynthesis Proto-Oncogene Proteins - physiology Receptor, Serotonin, 5-HT2C Receptors, Serotonin - biosynthesis Receptors, Serotonin - physiology Signal Transduction Vertebrates Wnt Proteins Wnt-5a Protein Zebrafish |
| title | Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A |
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