Kallikrein-binding protein levels are reduced in the retinas of streptozotocin-induced diabetic rats
To determine the involvement of rat kallikrein-binding protein (RKBP) in the development of diabetic retinopathy. Diabetes was induced by streptozotocin (STZ) (55 mg/kg body weight in 0.05 M citrate buffer, pH 4.5) in male Sprague-Dawley rats (150 to 175 g, 6 weeks old) as confirmed by hyperglycemia...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1997-03, Vol.38 (3), p.658-664 |
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| creator | Hatcher, HC Ma, JX Chao, J Chao, L Ottlecz, A |
| description | To determine the involvement of rat kallikrein-binding protein (RKBP) in the development of diabetic retinopathy.
Diabetes was induced by streptozotocin (STZ) (55 mg/kg body weight in 0.05 M citrate buffer, pH 4.5) in male Sprague-Dawley rats (150 to 175 g, 6 weeks old) as confirmed by hyperglycemia and reduced body weight. Retinas were dissected from animals at 1, 2, and 4 months of diabetes. The functional activity of RKBP in retinal homogenates was determined by its complex formation with tissue kallikrein. Immunoreactive RKBP levels were measured by enzyme-linked immunosorbent assay. The RKBP messenger RNA (mRNA) levels in the retina were measured by Northern blot analysis using the RKBP complementary DNA probe. The activity of total Na+,K(+)-ATPase was determined by a radioassay. Total protein concentration was determined by a protein assay.
The kallikrein-binding activity was reduced in the retinas of STZ-diabetic rats at 1 (59%), 2 (50%), and 4 (38%) months of diabetes compared to those of age-matched control subjects. Levels of immunoreactive RKBP were significantly lower in the diabetic animals at each time point examined compared to those of control subjects. At 1 and 2 months of diabetes, RKBP levels (nanogram/milligram protein) were decreased significantly to 6.9 +/- 0.7 (n = 8) and 10.6 +/- 1.0 (n = 8), respectively, compared to those of age-matched control subjects (14.1 +/- 0.7, n = 8, P < 0.001, and 14.1 +/- 1.2, n = 8, P < 0.01). At 4 months of diabetes, retinal RKBP levels were lower in both control and diabetic groups, but RKBP levels in diabetic groups were significantly lower (5.8 +/- 0.6, n = 8) than those of the age-matched control subjects (8.4 +/- 0.9, n = 8, P < 0.01). Similarly, Northern blot analysis showed that RKBP mRNA levels were reduced in the retina of each group of STZ-diabetic rats, suggesting that the decrease in RKBP occurred at the level of transcription.
The results show that STZ-induced diabetic rats have decreased retinal RKBP; moreover, this suggests that RKBP may contribute to diabetic retinopathy. |
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Diabetes was induced by streptozotocin (STZ) (55 mg/kg body weight in 0.05 M citrate buffer, pH 4.5) in male Sprague-Dawley rats (150 to 175 g, 6 weeks old) as confirmed by hyperglycemia and reduced body weight. Retinas were dissected from animals at 1, 2, and 4 months of diabetes. The functional activity of RKBP in retinal homogenates was determined by its complex formation with tissue kallikrein. Immunoreactive RKBP levels were measured by enzyme-linked immunosorbent assay. The RKBP messenger RNA (mRNA) levels in the retina were measured by Northern blot analysis using the RKBP complementary DNA probe. The activity of total Na+,K(+)-ATPase was determined by a radioassay. Total protein concentration was determined by a protein assay.
The kallikrein-binding activity was reduced in the retinas of STZ-diabetic rats at 1 (59%), 2 (50%), and 4 (38%) months of diabetes compared to those of age-matched control subjects. Levels of immunoreactive RKBP were significantly lower in the diabetic animals at each time point examined compared to those of control subjects. At 1 and 2 months of diabetes, RKBP levels (nanogram/milligram protein) were decreased significantly to 6.9 +/- 0.7 (n = 8) and 10.6 +/- 1.0 (n = 8), respectively, compared to those of age-matched control subjects (14.1 +/- 0.7, n = 8, P < 0.001, and 14.1 +/- 1.2, n = 8, P < 0.01). At 4 months of diabetes, retinal RKBP levels were lower in both control and diabetic groups, but RKBP levels in diabetic groups were significantly lower (5.8 +/- 0.6, n = 8) than those of the age-matched control subjects (8.4 +/- 0.9, n = 8, P < 0.01). Similarly, Northern blot analysis showed that RKBP mRNA levels were reduced in the retina of each group of STZ-diabetic rats, suggesting that the decrease in RKBP occurred at the level of transcription.
The results show that STZ-induced diabetic rats have decreased retinal RKBP; moreover, this suggests that RKBP may contribute to diabetic retinopathy.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 9071220</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Animals ; Associated diseases and complications ; Biological and medical sciences ; Blotting, Northern ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Diabetes Mellitus, Experimental - metabolism ; Diabetes. Impaired glucose tolerance ; Diabetic Retinopathy - metabolism ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Enzyme-Linked Immunosorbent Assay ; Kallikreins - genetics ; Kallikreins - metabolism ; Male ; Medical sciences ; Rats ; Rats, Sprague-Dawley ; Retina - metabolism ; RNA, Messenger - metabolism ; Serpins - genetics ; Serpins - metabolism ; Sodium-Potassium-Exchanging ATPase - metabolism ; Streptozocin</subject><ispartof>Investigative ophthalmology & visual science, 1997-03, Vol.38 (3), p.658-664</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2616604$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9071220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hatcher, HC</creatorcontrib><creatorcontrib>Ma, JX</creatorcontrib><creatorcontrib>Chao, J</creatorcontrib><creatorcontrib>Chao, L</creatorcontrib><creatorcontrib>Ottlecz, A</creatorcontrib><title>Kallikrein-binding protein levels are reduced in the retinas of streptozotocin-induced diabetic rats</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To determine the involvement of rat kallikrein-binding protein (RKBP) in the development of diabetic retinopathy.
Diabetes was induced by streptozotocin (STZ) (55 mg/kg body weight in 0.05 M citrate buffer, pH 4.5) in male Sprague-Dawley rats (150 to 175 g, 6 weeks old) as confirmed by hyperglycemia and reduced body weight. Retinas were dissected from animals at 1, 2, and 4 months of diabetes. The functional activity of RKBP in retinal homogenates was determined by its complex formation with tissue kallikrein. Immunoreactive RKBP levels were measured by enzyme-linked immunosorbent assay. The RKBP messenger RNA (mRNA) levels in the retina were measured by Northern blot analysis using the RKBP complementary DNA probe. The activity of total Na+,K(+)-ATPase was determined by a radioassay. Total protein concentration was determined by a protein assay.
The kallikrein-binding activity was reduced in the retinas of STZ-diabetic rats at 1 (59%), 2 (50%), and 4 (38%) months of diabetes compared to those of age-matched control subjects. Levels of immunoreactive RKBP were significantly lower in the diabetic animals at each time point examined compared to those of control subjects. At 1 and 2 months of diabetes, RKBP levels (nanogram/milligram protein) were decreased significantly to 6.9 +/- 0.7 (n = 8) and 10.6 +/- 1.0 (n = 8), respectively, compared to those of age-matched control subjects (14.1 +/- 0.7, n = 8, P < 0.001, and 14.1 +/- 1.2, n = 8, P < 0.01). At 4 months of diabetes, retinal RKBP levels were lower in both control and diabetic groups, but RKBP levels in diabetic groups were significantly lower (5.8 +/- 0.6, n = 8) than those of the age-matched control subjects (8.4 +/- 0.9, n = 8, P < 0.01). Similarly, Northern blot analysis showed that RKBP mRNA levels were reduced in the retina of each group of STZ-diabetic rats, suggesting that the decrease in RKBP occurred at the level of transcription.
The results show that STZ-induced diabetic rats have decreased retinal RKBP; moreover, this suggests that RKBP may contribute to diabetic retinopathy.</description><subject>Animals</subject><subject>Associated diseases and complications</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diabetic Retinopathy - metabolism</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Kallikreins - genetics</subject><subject>Kallikreins - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Retina - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Serpins - genetics</subject><subject>Serpins - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Streptozocin</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtKxDAUhoso4zj6CEIX6q6QS3OZpQzeUHCj65ImJ9NoL2OSWvTpjU5xdfjP__HBOQfZEjNGCiYkPcyWCJe8QCUqj7OTEN4QIhgTtMgWayQwIWiZmUfVtu7dg-uL2vXG9dt854eYct7CJ7QhVx5yD2bUYPK0jc1vjK5XIR9sHqKHXRy-hzjo5EiKP9A4VSdI517FcJodWdUGOJvnKnu9vXnZ3BdPz3cPm-unoiGcxQIQYRKAcVFzYNTa2hCtOLJUMCO05gwLiawWWHIMhhvAtJTWihIYs2hNV9nV3psu-BghxKpzQUPbqh6GMVRCSknZWiTwfAbHugNT7bzrlP-q5rek_mLuVdCqtV712oV_jHDMOSoTdrnHGrdtJuehCl16Z5LiapomKitacSbpD2zBesM</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>Hatcher, HC</creator><creator>Ma, JX</creator><creator>Chao, J</creator><creator>Chao, L</creator><creator>Ottlecz, A</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970301</creationdate><title>Kallikrein-binding protein levels are reduced in the retinas of streptozotocin-induced diabetic rats</title><author>Hatcher, HC ; Ma, JX ; Chao, J ; Chao, L ; Ottlecz, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-e0258ee567b6e53ffbd2ca60f375d7cc651780fc71861ed6de1348ff74e55f093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Associated diseases and complications</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diabetic Retinopathy - metabolism</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Kallikreins - genetics</topic><topic>Kallikreins - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Retina - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Serpins - genetics</topic><topic>Serpins - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Streptozocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hatcher, HC</creatorcontrib><creatorcontrib>Ma, JX</creatorcontrib><creatorcontrib>Chao, J</creatorcontrib><creatorcontrib>Chao, L</creatorcontrib><creatorcontrib>Ottlecz, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hatcher, HC</au><au>Ma, JX</au><au>Chao, J</au><au>Chao, L</au><au>Ottlecz, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kallikrein-binding protein levels are reduced in the retinas of streptozotocin-induced diabetic rats</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>38</volume><issue>3</issue><spage>658</spage><epage>664</epage><pages>658-664</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To determine the involvement of rat kallikrein-binding protein (RKBP) in the development of diabetic retinopathy.
Diabetes was induced by streptozotocin (STZ) (55 mg/kg body weight in 0.05 M citrate buffer, pH 4.5) in male Sprague-Dawley rats (150 to 175 g, 6 weeks old) as confirmed by hyperglycemia and reduced body weight. Retinas were dissected from animals at 1, 2, and 4 months of diabetes. The functional activity of RKBP in retinal homogenates was determined by its complex formation with tissue kallikrein. Immunoreactive RKBP levels were measured by enzyme-linked immunosorbent assay. The RKBP messenger RNA (mRNA) levels in the retina were measured by Northern blot analysis using the RKBP complementary DNA probe. The activity of total Na+,K(+)-ATPase was determined by a radioassay. Total protein concentration was determined by a protein assay.
The kallikrein-binding activity was reduced in the retinas of STZ-diabetic rats at 1 (59%), 2 (50%), and 4 (38%) months of diabetes compared to those of age-matched control subjects. Levels of immunoreactive RKBP were significantly lower in the diabetic animals at each time point examined compared to those of control subjects. At 1 and 2 months of diabetes, RKBP levels (nanogram/milligram protein) were decreased significantly to 6.9 +/- 0.7 (n = 8) and 10.6 +/- 1.0 (n = 8), respectively, compared to those of age-matched control subjects (14.1 +/- 0.7, n = 8, P < 0.001, and 14.1 +/- 1.2, n = 8, P < 0.01). At 4 months of diabetes, retinal RKBP levels were lower in both control and diabetic groups, but RKBP levels in diabetic groups were significantly lower (5.8 +/- 0.6, n = 8) than those of the age-matched control subjects (8.4 +/- 0.9, n = 8, P < 0.01). Similarly, Northern blot analysis showed that RKBP mRNA levels were reduced in the retina of each group of STZ-diabetic rats, suggesting that the decrease in RKBP occurred at the level of transcription.
The results show that STZ-induced diabetic rats have decreased retinal RKBP; moreover, this suggests that RKBP may contribute to diabetic retinopathy.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>9071220</pmid><tpages>7</tpages></addata></record> |
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| subjects | Animals Associated diseases and complications Biological and medical sciences Blotting, Northern Carrier Proteins - genetics Carrier Proteins - metabolism Diabetes Mellitus, Experimental - metabolism Diabetes. Impaired glucose tolerance Diabetic Retinopathy - metabolism Endocrine pancreas. Apud cells (diseases) Endocrinopathies Enzyme-Linked Immunosorbent Assay Kallikreins - genetics Kallikreins - metabolism Male Medical sciences Rats Rats, Sprague-Dawley Retina - metabolism RNA, Messenger - metabolism Serpins - genetics Serpins - metabolism Sodium-Potassium-Exchanging ATPase - metabolism Streptozocin |
| title | Kallikrein-binding protein levels are reduced in the retinas of streptozotocin-induced diabetic rats |
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