Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids
Virulent strains of Entamoeba histolytica have been reported to produce a mixture of phosphoglycoconjugates that share some structural features with the lipophosphoglycans (LPGs) of Leishmania. Purification of these glycoconjugates is essential to their precise structural characterization. In this s...
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| Veröffentlicht in: | Parasitology 1997-02, Vol.114 (2), p.95-104 |
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| creator | MOODY, S. BECKER, S. NUCHAMOWITZ, Y. MIRELMAN, D. |
| description | Virulent strains of Entamoeba histolytica have been
reported to produce a mixture of phosphoglycoconjugates that share
some structural features with the lipophosphoglycans (LPGs) of Leishmania.
Purification of these glycoconjugates is
essential to their precise structural characterization. In this study we
have
extracted ‘LPG-like’ molecules from various
virulent E. histolytica strains and purified on the basis of charge
differences, 2 apparently related glycoconjugates a ‘LPG’
and a ‘lipophosphopeptidoglycan (LPPG)’. In marked contrast
to the
abundance of these ‘LPG’ and ‘LPPG’ molecules
in the virulent strains, avirulent E. histolytica and E. dispar
strains produce either very low, or no detectable levels of LPG,
and either low levels or modified forms of ‘LPPG’. Monospecific
polyclonal antibodies prepared against that ‘LPG’ of
the virulent strain HM-1: IMSS cl6 identified epitopes shared between both
the
‘LPG’ and the ‘LPPG’ of this and other
virulent strains, using Western blot analysis. Flow cytometric analysis
of a
range of strains using these antibodies identified
a surface distribution of these molecules and confirmed a correlation between
surface exposure of epitopes bound by these
antibodies and parasite virulence. |
| doi_str_mv | 10.1017/S0031182096008396 |
| format | Article |
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reported to produce a mixture of phosphoglycoconjugates that share
some structural features with the lipophosphoglycans (LPGs) of Leishmania.
Purification of these glycoconjugates is
essential to their precise structural characterization. In this study we
have
extracted ‘LPG-like’ molecules from various
virulent E. histolytica strains and purified on the basis of charge
differences, 2 apparently related glycoconjugates a ‘LPG’
and a ‘lipophosphopeptidoglycan (LPPG)’. In marked contrast
to the
abundance of these ‘LPG’ and ‘LPPG’ molecules
in the virulent strains, avirulent E. histolytica and E. dispar
strains produce either very low, or no detectable levels of LPG,
and either low levels or modified forms of ‘LPPG’. Monospecific
polyclonal antibodies prepared against that ‘LPG’ of
the virulent strain HM-1: IMSS cl6 identified epitopes shared between both
the
‘LPG’ and the ‘LPPG’ of this and other
virulent strains, using Western blot analysis. Flow cytometric analysis
of a
range of strains using these antibodies identified
a surface distribution of these molecules and confirmed a correlation between
surface exposure of epitopes bound by these
antibodies and parasite virulence.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182096008396</identifier><identifier>PMID: 9051918</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Animals ; Antibodies, Protozoan ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Carbohydrates - analysis ; Cell Membrane - chemistry ; Electrophoresis, Polyacrylamide Gel ; Entamoeba - chemistry ; Entamoeba - classification ; Entamoeba - pathogenicity ; Entamoeba dispar ; Entamoeba histolytica ; Entamoeba histolytica - chemistry ; Entamoeba histolytica - classification ; Entamoeba histolytica - pathogenicity ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; Glycoconjugates - chemistry ; Glycoconjugates - isolation & purification ; Glycosphingolipids - chemistry ; Glycosphingolipids - isolation & purification ; Guinea Pigs ; lipophosphoglycan ; lipophosphopeptidoglycan ; Phosphatidylinositol Diacylglycerol-Lyase ; Phosphoric Diester Hydrolases ; Protozoa ; Species Specificity ; Virulence</subject><ispartof>Parasitology, 1997-02, Vol.114 (2), p.95-104</ispartof><rights>1997 Cambridge University Press</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-8229cd37d2a9c26db5d2b166e714ec68c31e7323fe4570941570780f2c23d8693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182096008396/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,315,781,785,27929,27930,55633</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2597214$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9051918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOODY, S.</creatorcontrib><creatorcontrib>BECKER, S.</creatorcontrib><creatorcontrib>NUCHAMOWITZ, Y.</creatorcontrib><creatorcontrib>MIRELMAN, D.</creatorcontrib><title>Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Virulent strains of Entamoeba histolytica have been
reported to produce a mixture of phosphoglycoconjugates that share
some structural features with the lipophosphoglycans (LPGs) of Leishmania.
Purification of these glycoconjugates is
essential to their precise structural characterization. In this study we
have
extracted ‘LPG-like’ molecules from various
virulent E. histolytica strains and purified on the basis of charge
differences, 2 apparently related glycoconjugates a ‘LPG’
and a ‘lipophosphopeptidoglycan (LPPG)’. In marked contrast
to the
abundance of these ‘LPG’ and ‘LPPG’ molecules
in the virulent strains, avirulent E. histolytica and E. dispar
strains produce either very low, or no detectable levels of LPG,
and either low levels or modified forms of ‘LPPG’. Monospecific
polyclonal antibodies prepared against that ‘LPG’ of
the virulent strain HM-1: IMSS cl6 identified epitopes shared between both
the
‘LPG’ and the ‘LPPG’ of this and other
virulent strains, using Western blot analysis. Flow cytometric analysis
of a
range of strains using these antibodies identified
a surface distribution of these molecules and confirmed a correlation between
surface exposure of epitopes bound by these
antibodies and parasite virulence.</description><subject>Animals</subject><subject>Antibodies, Protozoan</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates - analysis</subject><subject>Cell Membrane - chemistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Entamoeba - chemistry</subject><subject>Entamoeba - classification</subject><subject>Entamoeba - pathogenicity</subject><subject>Entamoeba dispar</subject><subject>Entamoeba histolytica</subject><subject>Entamoeba histolytica - chemistry</subject><subject>Entamoeba histolytica - classification</subject><subject>Entamoeba histolytica - pathogenicity</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoconjugates - chemistry</subject><subject>Glycoconjugates - isolation & purification</subject><subject>Glycosphingolipids - chemistry</subject><subject>Glycosphingolipids - isolation & purification</subject><subject>Guinea Pigs</subject><subject>lipophosphoglycan</subject><subject>lipophosphopeptidoglycan</subject><subject>Phosphatidylinositol Diacylglycerol-Lyase</subject><subject>Phosphoric Diester Hydrolases</subject><subject>Protozoa</subject><subject>Species Specificity</subject><subject>Virulence</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKtPCD2CB5AVil-JrJ34sUTW0hSKEeIid5dhOxyWv2gli_j0OE2aDBAv7yjrfPTryQegZkHMgIF59IoQBSEoUJ0QyxR-gDZRcFRI4PESbRS4W_TE6TemOEMIZpyfoRJEKFMgNil9DnFvfT9j0Dpsff17bfjLd4GuDdyFNQ7ufgjW_me05diGNJubRND7i0ONp50PE1rctTnNsjPV43A0pn7hvzeQdvm33dmjDGFx6gh41pk3-6TrP0Jc3288XV8XNh8vri9c3hS2ZnApJqbKOCUeNspS7unK0Bs69gNJbLi0DLxhljS8rQVQJ-RaSNNRS5iRX7Ay9PPiOcbiffZp0F9KS0fR-mJMWUoqqpNV_QeBUMVmJDMIBtHFIKfpGjzF0Ju41EL0Uov8qJO88X83nuvPuuLE2kPUXq26SNW0TTW9DOmK0UoJCmbHigOU2_M-jbOJ3zQUTleaXH_W78v3bK6i-6cWWrVFNV8fgbr2-G-bY5w__R9hfwiaxBw</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>MOODY, S.</creator><creator>BECKER, S.</creator><creator>NUCHAMOWITZ, Y.</creator><creator>MIRELMAN, D.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids</title><author>MOODY, S. ; BECKER, S. ; NUCHAMOWITZ, Y. ; MIRELMAN, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-8229cd37d2a9c26db5d2b166e714ec68c31e7323fe4570941570780f2c23d8693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibodies, Protozoan</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates - analysis</topic><topic>Cell Membrane - chemistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Entamoeba - chemistry</topic><topic>Entamoeba - classification</topic><topic>Entamoeba - pathogenicity</topic><topic>Entamoeba dispar</topic><topic>Entamoeba histolytica</topic><topic>Entamoeba histolytica - chemistry</topic><topic>Entamoeba histolytica - classification</topic><topic>Entamoeba histolytica - pathogenicity</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoconjugates - chemistry</topic><topic>Glycoconjugates - isolation & purification</topic><topic>Glycosphingolipids - chemistry</topic><topic>Glycosphingolipids - isolation & purification</topic><topic>Guinea Pigs</topic><topic>lipophosphoglycan</topic><topic>lipophosphopeptidoglycan</topic><topic>Phosphatidylinositol Diacylglycerol-Lyase</topic><topic>Phosphoric Diester Hydrolases</topic><topic>Protozoa</topic><topic>Species Specificity</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOODY, S.</creatorcontrib><creatorcontrib>BECKER, S.</creatorcontrib><creatorcontrib>NUCHAMOWITZ, Y.</creatorcontrib><creatorcontrib>MIRELMAN, D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOODY, S.</au><au>BECKER, S.</au><au>NUCHAMOWITZ, Y.</au><au>MIRELMAN, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>114</volume><issue>2</issue><spage>95</spage><epage>104</epage><pages>95-104</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Virulent strains of Entamoeba histolytica have been
reported to produce a mixture of phosphoglycoconjugates that share
some structural features with the lipophosphoglycans (LPGs) of Leishmania.
Purification of these glycoconjugates is
essential to their precise structural characterization. In this study we
have
extracted ‘LPG-like’ molecules from various
virulent E. histolytica strains and purified on the basis of charge
differences, 2 apparently related glycoconjugates a ‘LPG’
and a ‘lipophosphopeptidoglycan (LPPG)’. In marked contrast
to the
abundance of these ‘LPG’ and ‘LPPG’ molecules
in the virulent strains, avirulent E. histolytica and E. dispar
strains produce either very low, or no detectable levels of LPG,
and either low levels or modified forms of ‘LPPG’. Monospecific
polyclonal antibodies prepared against that ‘LPG’ of
the virulent strain HM-1: IMSS cl6 identified epitopes shared between both
the
‘LPG’ and the ‘LPPG’ of this and other
virulent strains, using Western blot analysis. Flow cytometric analysis
of a
range of strains using these antibodies identified
a surface distribution of these molecules and confirmed a correlation between
surface exposure of epitopes bound by these
antibodies and parasite virulence.</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><pmid>9051918</pmid><doi>10.1017/S0031182096008396</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-1820 |
| ispartof | Parasitology, 1997-02, Vol.114 (2), p.95-104 |
| issn | 0031-1820 1469-8161 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78875425 |
| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Antibodies, Protozoan Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Carbohydrates - analysis Cell Membrane - chemistry Electrophoresis, Polyacrylamide Gel Entamoeba - chemistry Entamoeba - classification Entamoeba - pathogenicity Entamoeba dispar Entamoeba histolytica Entamoeba histolytica - chemistry Entamoeba histolytica - classification Entamoeba histolytica - pathogenicity Epitopes - analysis Fundamental and applied biological sciences. Psychology Glycoconjugates - chemistry Glycoconjugates - isolation & purification Glycosphingolipids - chemistry Glycosphingolipids - isolation & purification Guinea Pigs lipophosphoglycan lipophosphopeptidoglycan Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases Protozoa Species Specificity Virulence |
| title | Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids |
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