Angiotensin-Converting Enzyme Inhibitor Up-Regulates Cardiac β-Receptors in Cultured Neonatal Rat Myocytes

In patients with congestive heart failure (CHF), β-receptor up-regulation is regarded as one of the mechanisms leading to improved function and prognosis. To clarify whether β-receptor up-regulation is involved in the mechanisms underlying the beneficial effects of angiotensin-converting enzyme (ACE...

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Veröffentlicht in:JAPANESE CIRCULATION JOURNAL 1997, Vol.61(2), pp.170-179
Hauptverfasser: Yonemochi, Hidetoshi, Saikawa, Tetsunori, Yasunaga, Seikou, Iwao, Tetsu, Takakura, Takeshi, Nakagawa, Mikiko, Sakata, Toshiie, Ito, Morio
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container_issue 2
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container_title JAPANESE CIRCULATION JOURNAL
container_volume 61
creator Yonemochi, Hidetoshi
Saikawa, Tetsunori
Yasunaga, Seikou
Iwao, Tetsu
Takakura, Takeshi
Nakagawa, Mikiko
Sakata, Toshiie
Ito, Morio
description In patients with congestive heart failure (CHF), β-receptor up-regulation is regarded as one of the mechanisms leading to improved function and prognosis. To clarify whether β-receptor up-regulation is involved in the mechanisms underlying the beneficial effects of angiotensin-converting enzyme (ACE) inhibitors, we investigated the actions of ACE inhibitors and an angiotensin II type I receptor (AT1) antagonist on β-receptors of neonatal rat cultured cardiac myocytes. Angiotensin II (A-II) increased the spontaneous beating frequency of the cells, and the effect was completely antagonized by the AT1 antagonist CV-11974. Under control conditions, β-receptor density (Bmax) and affinity (Kd) were measured by radiobinding assay with the hydrophilic ligand [3H]CGP-12177, and were 103 ±11 fmol/mg protein and 3.4±0.4 nmol/L, respectively. Captopril increased the β-receptor density of myocytes and augmented the response to isoproterenol. Bmax was increased by 34% after 24 h treatment with 10 -6 mol/L captopril. CV-3480, an ACE inhibitor that contains no sulthydryl group, but neither A-II nor the AT1 antagonist, also up-regulated β-receptors. The results suggest that β-receptor up-regulation contributes at least partly to the beneficial cardiac effects of ACE inhibitors in patients with CHF. ACE inhibitors and AT1 antagonists seem to play different roles in clinical practice. (Jpn Circ J 1997; 61: 170 -179)
doi_str_mv 10.1253/jcj.61.170
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To clarify whether β-receptor up-regulation is involved in the mechanisms underlying the beneficial effects of angiotensin-converting enzyme (ACE) inhibitors, we investigated the actions of ACE inhibitors and an angiotensin II type I receptor (AT1) antagonist on β-receptors of neonatal rat cultured cardiac myocytes. Angiotensin II (A-II) increased the spontaneous beating frequency of the cells, and the effect was completely antagonized by the AT1 antagonist CV-11974. Under control conditions, β-receptor density (Bmax) and affinity (Kd) were measured by radiobinding assay with the hydrophilic ligand [3H]CGP-12177, and were 103 ±11 fmol/mg protein and 3.4±0.4 nmol/L, respectively. Captopril increased the β-receptor density of myocytes and augmented the response to isoproterenol. Bmax was increased by 34% after 24 h treatment with 10 -6 mol/L captopril. CV-3480, an ACE inhibitor that contains no sulthydryl group, but neither A-II nor the AT1 antagonist, also up-regulated β-receptors. The results suggest that β-receptor up-regulation contributes at least partly to the beneficial cardiac effects of ACE inhibitors in patients with CHF. ACE inhibitors and AT1 antagonists seem to play different roles in clinical practice. 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The results suggest that β-receptor up-regulation contributes at least partly to the beneficial cardiac effects of ACE inhibitors in patients with CHF. ACE inhibitors and AT1 antagonists seem to play different roles in clinical practice. 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Drug treatments</subject><subject>Radioligand Assay</subject><subject>Rats</subject><subject>Receptors, Adrenergic, beta - metabolism</subject><subject>Tetrazoles - pharmacology</subject><subject>Up-Regulation</subject><subject>Vasodilator agents. 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subjects Angiotensin II
Angiotensin II - pharmacology
Angiotensin II receptor antagonist
Angiotensin Receptor Antagonists
Angiotensin-Converting Enzyme Inhibitors - pharmacology
Animals
Animals, Newborn
b-Adrenergic receptor
Benzimidazoles - pharmacology
Biological and medical sciences
Captopril - pharmacology
Cardiovascular system
Cells, Cultured
Congestive heart failure
Medical sciences
Myocardial Contraction - drug effects
Myocardium - cytology
Myocardium - metabolism
Pharmacology. Drug treatments
Radioligand Assay
Rats
Receptors, Adrenergic, beta - metabolism
Tetrazoles - pharmacology
Up-Regulation
Vasodilator agents. Cerebral vasodilators
title Angiotensin-Converting Enzyme Inhibitor Up-Regulates Cardiac β-Receptors in Cultured Neonatal Rat Myocytes
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