Determination of the nucleotide conformation in the productive enzyme-substrate complexes of RNA-depolymerases

The aim of this work is to determine the conformation of the nucleobase adjacent to the cleavable phosphodiester bond in the productive enzyme-substrate complex of RNA-depolymerizing enzymes. To this end the kinetic parameters of hydrolysis of UpA, 2′-C-Me- and 3′-C-Me-UpA were determined for RNase...

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Veröffentlicht in:FEBS letters 1997-03, Vol.404 (2), p.169-172
Hauptverfasser: Moiseyev, G.P, Yakovlev, G.I, Lysov, Yu.P, Chernyi, A.A, Polyakov, K.M, Oivanen, M, Lönnberg, H, Beigelman, L.N, Efimtseva, E.V, Mikhailov, S.N
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container_end_page 172
container_issue 2
container_start_page 169
container_title FEBS letters
container_volume 404
creator Moiseyev, G.P
Yakovlev, G.I
Lysov, Yu.P
Chernyi, A.A
Polyakov, K.M
Oivanen, M
Lönnberg, H
Beigelman, L.N
Efimtseva, E.V
Mikhailov, S.N
description The aim of this work is to determine the conformation of the nucleobase adjacent to the cleavable phosphodiester bond in the productive enzyme-substrate complex of RNA-depolymerizing enzymes. To this end the kinetic parameters of hydrolysis of UpA, 2′-C-Me- and 3′-C-Me-UpA were determined for RNase A, RNase Pb 2, nuclease S 1 and snake venom phosphodiesterase. In these derivatives the ranges of the allowed orientation of uridine residues are restricted due to the substitution of methyl groups for the ribose hydrogen atoms. The results described demonstrate that the proposed method is of general value for the estimation of the nucleotide glycoside angles in the productive enzyme-substrate complexes. © 1997 Federation of European Biochemical Societies.
doi_str_mv 10.1016/S0014-5793(97)00092-6
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subjects Animals
Calorimetry
Cattle
Conformation of substrate
C′-methyl analogues of UpA
Dinucleoside Phosphates - chemistry
Dinucleoside Phosphates - metabolism
Nucleic Acid Conformation
Penicillium - enzymology
Phosphoric Diester Hydrolases - chemistry
Phosphoric Diester Hydrolases - metabolism
Protein Conformation
Ribonuclease, Pancreatic - chemistry
Ribonuclease, Pancreatic - metabolism
Ribonucleases - chemistry
Ribonucleases - metabolism
RNA-depolymerizing enzyme
Single-Strand Specific DNA and RNA Endonucleases - chemistry
Single-Strand Specific DNA and RNA Endonucleases - metabolism
Snake Venoms
title Determination of the nucleotide conformation in the productive enzyme-substrate complexes of RNA-depolymerases
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