cAMP- and calcium-mediated modulation of intrinsic factor secretion in isolated rabbit gastric glands
We have studied the role of cAMP-mediated and calcium-mediated secretagogues in stimulating secretion of intrinsic factor (IF) from parietal cells in isolated rabbit gastric glands. The magnitudes of IF release caused by maximally stimulating doses of the following agents were compared: histamine (5...
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| Veröffentlicht in: | The Journal of surgical research 1989-03, Vol.46 (3), p.241-245 |
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| creator | Ballantyne, Garth H. Zdon, Michael J. Zucker, Karl A. Modlin, Irvin M. |
| description | We have studied the role of cAMP-mediated and calcium-mediated secretagogues in stimulating secretion of intrinsic factor (IF) from parietal cells in isolated rabbit gastric glands. The magnitudes of IF release caused by maximally stimulating doses of the following agents were compared: histamine (5 × 10
−5
M), forskolin (10
−5
M), 8-bromo-cAMP (10
−3
M), pentagastrin (10
−7
M), carbachol (10
−5
M), and the calcium ionophore A23187 (10
−3
M). Each agent was tested simultaneously in gastric glands prepared from the same animal so as to minimize the effects of variations between preparations. Gastric glands were incubated 30 min alone (unstimulated) or with each of the secretagogues. IF release into the culture medium was measured using an assay based on the binding of IF to
57Co-cyanocobalamin. Results were expressed as percentages IF release above unstimulated levels. Three cAMP-mediated agents significantly (
P < 0.05) stimulated IF secretion above unstimulated levels: histamine (439 ± 33%), forskolin (769 ± 385%), and 8-bromo-cAMP (1483 ± 362%). Two agents thought to act through calcium-dependent mechanisms significantly (
P < 0.05) stimulated IF release: carbachol (79 ± 25%) and pentagastrin (16 ± 6%). A23187 did not increase IF release above unstimulated levels (
P > 0.05). In this study, cAMP-mediated secretagogues stimulated significantly (
P < 0.05) more IF release than carbachol or pentagastrin. This study supports the hypothesis that both cAMP-and calcium-mediated mechanisms participate in regulating IF release. |
| doi_str_mv | 10.1016/0022-4804(89)90064-4 |
| format | Article |
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−5
M), forskolin (10
−5
M), 8-bromo-cAMP (10
−3
M), pentagastrin (10
−7
M), carbachol (10
−5
M), and the calcium ionophore A23187 (10
−3
M). Each agent was tested simultaneously in gastric glands prepared from the same animal so as to minimize the effects of variations between preparations. Gastric glands were incubated 30 min alone (unstimulated) or with each of the secretagogues. IF release into the culture medium was measured using an assay based on the binding of IF to
57Co-cyanocobalamin. Results were expressed as percentages IF release above unstimulated levels. Three cAMP-mediated agents significantly (
P < 0.05) stimulated IF secretion above unstimulated levels: histamine (439 ± 33%), forskolin (769 ± 385%), and 8-bromo-cAMP (1483 ± 362%). Two agents thought to act through calcium-dependent mechanisms significantly (
P < 0.05) stimulated IF release: carbachol (79 ± 25%) and pentagastrin (16 ± 6%). A23187 did not increase IF release above unstimulated levels (
P > 0.05). In this study, cAMP-mediated secretagogues stimulated significantly (
P < 0.05) more IF release than carbachol or pentagastrin. This study supports the hypothesis that both cAMP-and calcium-mediated mechanisms participate in regulating IF release.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/0022-4804(89)90064-4</identifier><identifier>PMID: 2537911</identifier><identifier>CODEN: JSGRA2</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology ; Animals ; Biological and medical sciences ; Calcimycin - pharmacology ; Calcium - physiology ; Carbachol - pharmacology ; Colforsin - pharmacology ; Cyclic AMP - physiology ; Fundamental and applied biological sciences. Psychology ; Gastrointestinal hormones ; Histamine - pharmacology ; In Vitro Techniques ; Intrinsic Factor - metabolism ; Parietal Cells, Gastric - drug effects ; Parietal Cells, Gastric - metabolism ; Pentagastrin - pharmacology ; Rabbits ; Vertebrates: digestive system</subject><ispartof>The Journal of surgical research, 1989-03, Vol.46 (3), p.241-245</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c250t-97b89fb9df8c197ca0bfb57e3c1a608254c5d72e2f8c908b33e55692ee082deb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-4804(89)90064-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6753690$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2537911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ballantyne, Garth H.</creatorcontrib><creatorcontrib>Zdon, Michael J.</creatorcontrib><creatorcontrib>Zucker, Karl A.</creatorcontrib><creatorcontrib>Modlin, Irvin M.</creatorcontrib><title>cAMP- and calcium-mediated modulation of intrinsic factor secretion in isolated rabbit gastric glands</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>We have studied the role of cAMP-mediated and calcium-mediated secretagogues in stimulating secretion of intrinsic factor (IF) from parietal cells in isolated rabbit gastric glands. The magnitudes of IF release caused by maximally stimulating doses of the following agents were compared: histamine (5 × 10
−5
M), forskolin (10
−5
M), 8-bromo-cAMP (10
−3
M), pentagastrin (10
−7
M), carbachol (10
−5
M), and the calcium ionophore A23187 (10
−3
M). Each agent was tested simultaneously in gastric glands prepared from the same animal so as to minimize the effects of variations between preparations. Gastric glands were incubated 30 min alone (unstimulated) or with each of the secretagogues. IF release into the culture medium was measured using an assay based on the binding of IF to
57Co-cyanocobalamin. Results were expressed as percentages IF release above unstimulated levels. Three cAMP-mediated agents significantly (
P < 0.05) stimulated IF secretion above unstimulated levels: histamine (439 ± 33%), forskolin (769 ± 385%), and 8-bromo-cAMP (1483 ± 362%). Two agents thought to act through calcium-dependent mechanisms significantly (
P < 0.05) stimulated IF release: carbachol (79 ± 25%) and pentagastrin (16 ± 6%). A23187 did not increase IF release above unstimulated levels (
P > 0.05). In this study, cAMP-mediated secretagogues stimulated significantly (
P < 0.05) more IF release than carbachol or pentagastrin. This study supports the hypothesis that both cAMP-and calcium-mediated mechanisms participate in regulating IF release.</description><subject>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcimycin - pharmacology</subject><subject>Calcium - physiology</subject><subject>Carbachol - pharmacology</subject><subject>Colforsin - pharmacology</subject><subject>Cyclic AMP - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrointestinal hormones</subject><subject>Histamine - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Intrinsic Factor - metabolism</subject><subject>Parietal Cells, Gastric - drug effects</subject><subject>Parietal Cells, Gastric - metabolism</subject><subject>Pentagastrin - pharmacology</subject><subject>Rabbits</subject><subject>Vertebrates: digestive system</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1q3DAURkVpSCdp3yAFLUpIFk4l27KkTWEI-YMJ7SJZC-n6OijYVirZgb59NDNmlgWBEN_5ri6HkDPOrjjjzU_GyrKoFasvlL7UjDV1UX8iK860KFQjq89kdUC-kJOUXll-a1kdk-NSVFJzviII68c_BbVjS8H24OehGLD1dsKWDqGdezv5MNLQUT9O0Y_JA-0sTCHShBBxl_p8Uuh3pWid8xN9sSnjQF_6PDp9JUed7RN-W-5T8nx783R9X2x-3z1crzcFlIJNhZZO6c7ptlPAtQTLXOeExAq4bZgqRQ2ilSWWOddMuapCIRpdIuawRVedkvP93LcY_s6YJjP4BNjnJTDMyUilJJdSZLDegxBDShE78xb9YOM_w5nZ2jVbdWarzihtdnZNnWvfl_mzy5oOpUVnzn8suU1ZZxftCD4dsCb_3GiWsV97DLOLd4_RJPA4QhYfESbTBv__PT4AeiOWwQ</recordid><startdate>198903</startdate><enddate>198903</enddate><creator>Ballantyne, Garth H.</creator><creator>Zdon, Michael J.</creator><creator>Zucker, Karl A.</creator><creator>Modlin, Irvin M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198903</creationdate><title>cAMP- and calcium-mediated modulation of intrinsic factor secretion in isolated rabbit gastric glands</title><author>Ballantyne, Garth H. ; Zdon, Michael J. ; Zucker, Karl A. ; Modlin, Irvin M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c250t-97b89fb9df8c197ca0bfb57e3c1a608254c5d72e2f8c908b33e55692ee082deb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>8-Bromo Cyclic Adenosine Monophosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcimycin - pharmacology</topic><topic>Calcium - physiology</topic><topic>Carbachol - pharmacology</topic><topic>Colforsin - pharmacology</topic><topic>Cyclic AMP - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrointestinal hormones</topic><topic>Histamine - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Intrinsic Factor - metabolism</topic><topic>Parietal Cells, Gastric - drug effects</topic><topic>Parietal Cells, Gastric - metabolism</topic><topic>Pentagastrin - pharmacology</topic><topic>Rabbits</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ballantyne, Garth H.</creatorcontrib><creatorcontrib>Zdon, Michael J.</creatorcontrib><creatorcontrib>Zucker, Karl A.</creatorcontrib><creatorcontrib>Modlin, Irvin M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ballantyne, Garth H.</au><au>Zdon, Michael J.</au><au>Zucker, Karl A.</au><au>Modlin, Irvin M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cAMP- and calcium-mediated modulation of intrinsic factor secretion in isolated rabbit gastric glands</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>1989-03</date><risdate>1989</risdate><volume>46</volume><issue>3</issue><spage>241</spage><epage>245</epage><pages>241-245</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><coden>JSGRA2</coden><abstract>We have studied the role of cAMP-mediated and calcium-mediated secretagogues in stimulating secretion of intrinsic factor (IF) from parietal cells in isolated rabbit gastric glands. The magnitudes of IF release caused by maximally stimulating doses of the following agents were compared: histamine (5 × 10
−5
M), forskolin (10
−5
M), 8-bromo-cAMP (10
−3
M), pentagastrin (10
−7
M), carbachol (10
−5
M), and the calcium ionophore A23187 (10
−3
M). Each agent was tested simultaneously in gastric glands prepared from the same animal so as to minimize the effects of variations between preparations. Gastric glands were incubated 30 min alone (unstimulated) or with each of the secretagogues. IF release into the culture medium was measured using an assay based on the binding of IF to
57Co-cyanocobalamin. Results were expressed as percentages IF release above unstimulated levels. Three cAMP-mediated agents significantly (
P < 0.05) stimulated IF secretion above unstimulated levels: histamine (439 ± 33%), forskolin (769 ± 385%), and 8-bromo-cAMP (1483 ± 362%). Two agents thought to act through calcium-dependent mechanisms significantly (
P < 0.05) stimulated IF release: carbachol (79 ± 25%) and pentagastrin (16 ± 6%). A23187 did not increase IF release above unstimulated levels (
P > 0.05). In this study, cAMP-mediated secretagogues stimulated significantly (
P < 0.05) more IF release than carbachol or pentagastrin. This study supports the hypothesis that both cAMP-and calcium-mediated mechanisms participate in regulating IF release.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2537911</pmid><doi>10.1016/0022-4804(89)90064-4</doi><tpages>5</tpages></addata></record> |
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| language | eng |
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| subjects | 8-Bromo Cyclic Adenosine Monophosphate - pharmacology Animals Biological and medical sciences Calcimycin - pharmacology Calcium - physiology Carbachol - pharmacology Colforsin - pharmacology Cyclic AMP - physiology Fundamental and applied biological sciences. Psychology Gastrointestinal hormones Histamine - pharmacology In Vitro Techniques Intrinsic Factor - metabolism Parietal Cells, Gastric - drug effects Parietal Cells, Gastric - metabolism Pentagastrin - pharmacology Rabbits Vertebrates: digestive system |
| title | cAMP- and calcium-mediated modulation of intrinsic factor secretion in isolated rabbit gastric glands |
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