H2O2 increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin
U. M. Wells, S. Duneclift, and J. G. Widdicombe Department of Physiology, St. George's Hospital Medical School, London SW17 ORE, United Kingdom Received 15 April 1996; accepted in final form 24 September 1996. Wells, U. M., S. Duneclift, and J. G. Widdicombe. H 2 O 2 increases sheep tracheal bl...
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| creator | Wells, U. M Duneclift, S Widdicombe, J. G |
| description | U. M.
Wells,
S.
Duneclift, and
J. G.
Widdicombe
Department of Physiology, St. George's Hospital Medical School,
London SW17 ORE, United Kingdom
Received 15 April 1996; accepted in final form 24 September 1996.
Wells, U. M., S. Duneclift, and J. G. Widdicombe.
H 2 O 2
increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin. J. Appl.
Physiol. 82(2): 621-631, 1997. Exogenous hydrogen
peroxide
(H 2 O 2 )
causes airway epithelial damage in vitro. We have studied the effects
of luminal
H 2 O 2
in the sheep trachea in vivo on tracheal permeability to
low-molecular-weight hydrophilic (technetium-99m-labeled
diethylenetriamine pentaacetic acid;
99m Tc-DTPA) and lipophilic
([ 14 C]antipyrine;
[ 14 C]AP) tracers and
on the tracheal vascular response to luminal capsaicin, which
stimulates afferent nerve endings. A tracheal artery was perfused, and
tracheal venous blood was collected. H 2 O 2
exposure (10 mM) reduced tracheal potential difference
( 42.0 ± 6.4 mV) to zero. It increased arterial and
venous flows (56.7 ± 6.1 and 57.3 ± 10.0%,
respectively; n = 5, P |
| doi_str_mv | 10.1152/jappl.1997.82.2.621 |
| format | Article |
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Wells,
S.
Duneclift, and
J. G.
Widdicombe
Department of Physiology, St. George's Hospital Medical School,
London SW17 ORE, United Kingdom
Received 15 April 1996; accepted in final form 24 September 1996.
Wells, U. M., S. Duneclift, and J. G. Widdicombe.
H 2 O 2
increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin. J. Appl.
Physiol. 82(2): 621-631, 1997. Exogenous hydrogen
peroxide
(H 2 O 2 )
causes airway epithelial damage in vitro. We have studied the effects
of luminal
H 2 O 2
in the sheep trachea in vivo on tracheal permeability to
low-molecular-weight hydrophilic (technetium-99m-labeled
diethylenetriamine pentaacetic acid;
99m Tc-DTPA) and lipophilic
([ 14 C]antipyrine;
[ 14 C]AP) tracers and
on the tracheal vascular response to luminal capsaicin, which
stimulates afferent nerve endings. A tracheal artery was perfused, and
tracheal venous blood was collected. H 2 O 2
exposure (10 mM) reduced tracheal potential difference
( 42.0 ± 6.4 mV) to zero. It increased arterial and
venous flows (56.7 ± 6.1 and 57.3 ± 10.0%,
respectively; n = 5, P < 0.01, paired t -test) but not tracheal lymph flow
(unstimulated flow 5.0 ± 1.2 µl · min 1 · cm 1 ,
n = 4). During
H 2 O 2
exposure, permeability to 99m Tc-DTPA increased from
2.6 to 89.7 × 10 7 cm/s
( n = 5, P < 0.05), whereas permeability to
[ 14 C]AP ( 3,312.6 × 10 7 cm/s,
n = 4) was not altered significantly
( 2,565 × 10 7
cm/s). Luminal capsaicin (10 µM) increased tracheal blood flow (10.1 ± 4.1%, n = 5)
and decreased venous 99m Tc-DTPA
concentration ( 19.7 ± 4.0, P < 0.01), and these effects were
significantly greater after epithelial damage (28.1 ± 6.0 and
45.7 ± 4.3%, respectively,
P < 0.05, unpaired
t -test). Thus H 2 O 2
increases the penetration of a hydrophilic tracer into tracheal blood
and lymph but has less effect on a lipophilic tracer. It also enhances
the effects of luminal capsaicin on blood flow and tracer uptake.
tracheal lymph; hydrogen peroxide; vascular hyperresponsiveness; potential difference
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.1997.82.2.621</identifier><identifier>PMID: 9049746</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Animals ; Biological and medical sciences ; Capsaicin - pharmacology ; Drug toxicity and drugs side effects treatment ; Hydrogen Peroxide - pharmacology ; Medical sciences ; Permeability - drug effects ; Pharmacology. Drug treatments ; Pulmonary Circulation - drug effects ; Sheep ; Toxicity: respiratory system, ent, stomatology ; Trachea - drug effects</subject><ispartof>Journal of applied physiology (1985), 1997-02, Vol.82 (2), p.621-631</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2587710$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9049746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wells, U. M</creatorcontrib><creatorcontrib>Duneclift, S</creatorcontrib><creatorcontrib>Widdicombe, J. G</creatorcontrib><title>H2O2 increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>U. M.
Wells,
S.
Duneclift, and
J. G.
Widdicombe
Department of Physiology, St. George's Hospital Medical School,
London SW17 ORE, United Kingdom
Received 15 April 1996; accepted in final form 24 September 1996.
Wells, U. M., S. Duneclift, and J. G. Widdicombe.
H 2 O 2
increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin. J. Appl.
Physiol. 82(2): 621-631, 1997. Exogenous hydrogen
peroxide
(H 2 O 2 )
causes airway epithelial damage in vitro. We have studied the effects
of luminal
H 2 O 2
in the sheep trachea in vivo on tracheal permeability to
low-molecular-weight hydrophilic (technetium-99m-labeled
diethylenetriamine pentaacetic acid;
99m Tc-DTPA) and lipophilic
([ 14 C]antipyrine;
[ 14 C]AP) tracers and
on the tracheal vascular response to luminal capsaicin, which
stimulates afferent nerve endings. A tracheal artery was perfused, and
tracheal venous blood was collected. H 2 O 2
exposure (10 mM) reduced tracheal potential difference
( 42.0 ± 6.4 mV) to zero. It increased arterial and
venous flows (56.7 ± 6.1 and 57.3 ± 10.0%,
respectively; n = 5, P < 0.01, paired t -test) but not tracheal lymph flow
(unstimulated flow 5.0 ± 1.2 µl · min 1 · cm 1 ,
n = 4). During
H 2 O 2
exposure, permeability to 99m Tc-DTPA increased from
2.6 to 89.7 × 10 7 cm/s
( n = 5, P < 0.05), whereas permeability to
[ 14 C]AP ( 3,312.6 × 10 7 cm/s,
n = 4) was not altered significantly
( 2,565 × 10 7
cm/s). Luminal capsaicin (10 µM) increased tracheal blood flow (10.1 ± 4.1%, n = 5)
and decreased venous 99m Tc-DTPA
concentration ( 19.7 ± 4.0, P < 0.01), and these effects were
significantly greater after epithelial damage (28.1 ± 6.0 and
45.7 ± 4.3%, respectively,
P < 0.05, unpaired
t -test). Thus H 2 O 2
increases the penetration of a hydrophilic tracer into tracheal blood
and lymph but has less effect on a lipophilic tracer. It also enhances
the effects of luminal capsaicin on blood flow and tracer uptake.
tracheal lymph; hydrogen peroxide; vascular hyperresponsiveness; potential difference
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Capsaicin - pharmacology</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Medical sciences</subject><subject>Permeability - drug effects</subject><subject>Pharmacology. Drug treatments</subject><subject>Pulmonary Circulation - drug effects</subject><subject>Sheep</subject><subject>Toxicity: respiratory system, ent, stomatology</subject><subject>Trachea - drug effects</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1TAQhi0EKofCEyAkLxBsmuBbYmeJKkqRKnVT1tbEdhpXTmzspOW8PZZ6YIc0mln83z-aC0LvKWkp7diXB0gptHQYZKtYy9qe0RfoUBXW0J7Ql-igZEca2Sn5Gr0p5YEQKkRHz9DZQMQgRX9A4zW7ZdivJjsoruAyO5fwlsHMDgIeQ4wWTyE-XeDk8uJg9MFvxwsMq8WPUMweIOPsSoprcXiLOOyLX6vVQCrgjV_folcThOLeneo5-nn17e7yurm5_f7j8utNM3NKt0bVMR0IyXsJyhoxWmUtmagShMJEpeAdsTVgqplbAZYz5Qzvph4YHwZ-jj499005_tpd2fTii3EhwOriXrRUStKe0wp-OIH7uDirU_YL5KM-HaXqH0963Q_ClGE1vvzDWB1UUlIx_ozN_n5-8tnpNB-LjyHeH_XVHsKd-73p-iTFNNP1OTrZqbo-_99VYf2X5n8Au-mTGA</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Wells, U. M</creator><creator>Duneclift, S</creator><creator>Widdicombe, J. G</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>H2O2 increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin</title><author>Wells, U. M ; Duneclift, S ; Widdicombe, J. G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h311t-8587ea47367a8dc4bd8dd0f18401af174350d50dafd503d4ad328ec35f6a23993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capsaicin - pharmacology</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Medical sciences</topic><topic>Permeability - drug effects</topic><topic>Pharmacology. Drug treatments</topic><topic>Pulmonary Circulation - drug effects</topic><topic>Sheep</topic><topic>Toxicity: respiratory system, ent, stomatology</topic><topic>Trachea - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wells, U. M</creatorcontrib><creatorcontrib>Duneclift, S</creatorcontrib><creatorcontrib>Widdicombe, J. G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wells, U. M</au><au>Duneclift, S</au><au>Widdicombe, J. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>H2O2 increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>82</volume><issue>2</issue><spage>621</spage><epage>631</epage><pages>621-631</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>U. M.
Wells,
S.
Duneclift, and
J. G.
Widdicombe
Department of Physiology, St. George's Hospital Medical School,
London SW17 ORE, United Kingdom
Received 15 April 1996; accepted in final form 24 September 1996.
Wells, U. M., S. Duneclift, and J. G. Widdicombe.
H 2 O 2
increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin. J. Appl.
Physiol. 82(2): 621-631, 1997. Exogenous hydrogen
peroxide
(H 2 O 2 )
causes airway epithelial damage in vitro. We have studied the effects
of luminal
H 2 O 2
in the sheep trachea in vivo on tracheal permeability to
low-molecular-weight hydrophilic (technetium-99m-labeled
diethylenetriamine pentaacetic acid;
99m Tc-DTPA) and lipophilic
([ 14 C]antipyrine;
[ 14 C]AP) tracers and
on the tracheal vascular response to luminal capsaicin, which
stimulates afferent nerve endings. A tracheal artery was perfused, and
tracheal venous blood was collected. H 2 O 2
exposure (10 mM) reduced tracheal potential difference
( 42.0 ± 6.4 mV) to zero. It increased arterial and
venous flows (56.7 ± 6.1 and 57.3 ± 10.0%,
respectively; n = 5, P < 0.01, paired t -test) but not tracheal lymph flow
(unstimulated flow 5.0 ± 1.2 µl · min 1 · cm 1 ,
n = 4). During
H 2 O 2
exposure, permeability to 99m Tc-DTPA increased from
2.6 to 89.7 × 10 7 cm/s
( n = 5, P < 0.05), whereas permeability to
[ 14 C]AP ( 3,312.6 × 10 7 cm/s,
n = 4) was not altered significantly
( 2,565 × 10 7
cm/s). Luminal capsaicin (10 µM) increased tracheal blood flow (10.1 ± 4.1%, n = 5)
and decreased venous 99m Tc-DTPA
concentration ( 19.7 ± 4.0, P < 0.01), and these effects were
significantly greater after epithelial damage (28.1 ± 6.0 and
45.7 ± 4.3%, respectively,
P < 0.05, unpaired
t -test). Thus H 2 O 2
increases the penetration of a hydrophilic tracer into tracheal blood
and lymph but has less effect on a lipophilic tracer. It also enhances
the effects of luminal capsaicin on blood flow and tracer uptake.
tracheal lymph; hydrogen peroxide; vascular hyperresponsiveness; potential difference
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>9049746</pmid><doi>10.1152/jappl.1997.82.2.621</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Capsaicin - pharmacology Drug toxicity and drugs side effects treatment Hydrogen Peroxide - pharmacology Medical sciences Permeability - drug effects Pharmacology. Drug treatments Pulmonary Circulation - drug effects Sheep Toxicity: respiratory system, ent, stomatology Trachea - drug effects |
| title | H2O2 increases sheep tracheal blood flow, permeability, and vascular response to luminal capsaicin |
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