Mucosal Immunity Induced by Parenteral Immunization with a Live Attenuated Venezuelan Equine Encephalitis Virus Vaccine Candidate
Induction of a mucosal immune response is generally thought to require introduction of an immunogen directly onto the mucosal surface. It has been observed, however, that live, attenuated mutants of the alphavirus, Venezuelan equine encephalitis virus (VEE), induce protection from virulent challenge...
Gespeichert in:
| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1997-02, Vol.228 (2), p.153-160 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 160 |
|---|---|
| container_issue | 2 |
| container_start_page | 153 |
| container_title | Virology (New York, N.Y.) |
| container_volume | 228 |
| creator | Charles, Peter C Brown, Kevin W Davis, Nancy L Hart, Mary Kate Johnston, Robert E |
| description | Induction of a mucosal immune response is generally thought to require introduction of an immunogen directly onto the mucosal surface. It has been observed, however, that live, attenuated mutants of the alphavirus, Venezuelan equine encephalitis virus (VEE), induce protection from virulent challenge at the respiratory mucosa even after parenteral inoculation. In this report, we propose a mechanism by which subcutaneous immunization with a molecularly cloned, attenuated double mutant of VEE is able to stimulate the production of mucosal anti-VEE IgA. Our results showed that the immunizing virus spread to, and replicated within, lymphoid tissues throughout the mouse. Several tissues known to be inductive sites of the mucosal immune system were found to be positive for the presence of VEE RNA by 48 hr postimmunization. Moreover, this mucosal lymphotropism resulted in the production of virus-specific IgA antibody detectable in vaginal secretions of immunized mice. |
| doi_str_mv | 10.1006/viro.1996.8381 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78870159</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0042682296983816</els_id><sourcerecordid>16287529</sourcerecordid><originalsourceid>FETCH-LOGICAL-c429t-f3f21ab6dfcd083e3eb1a0214190297ec4bffef399f2611b8cf17abd25f7ca3</originalsourceid><addsrcrecordid>eNqFkc9rFDEcxYModVu9ehCEnLzNmmRmJ8mxLKtdWFGo9hoymW9sZCazzY8t25v_uRl26U28JITPe-8bvg-hd5QsKSHtp4ML05JK2S5FLegLtKBEthWpG_oSLQhpWNUKxl6jyxh_k_LmnFygC0lZLRhdoD9fs5miHvB2HLN36Yi3vs8Getwd8XcdwCcIz_hJJzd5_OjSPdZ45w6Ar1MCn3Uqjjvw8JRh0B5vHrLzgDfewP5eDy65iO9cyOXUxsxorX3v-uJ7g15ZPUR4e76v0O3nzY_1TbX79mW7vt5VpmEyVba2jOqu7a3piaihho5qwmhDJWGSg2k6a8HWUlrWUtoJYynXXc9WlhtdX6GPp9R9mB4yxKRGFw0M5bMw5ai4EJzQlfyvkLZM8BWbhcuT0IQpxgBW7YMbdTgqStRcjZqrUXM1aq6mGD6ck3M3Qv8sP3dR-PsTt3pS-ldwUf28lbwuCbxAcYJQVnRwEFQ0Dsp6exfAJNVP7l9z_wLvQqjv</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16287529</pqid></control><display><type>article</type><title>Mucosal Immunity Induced by Parenteral Immunization with a Live Attenuated Venezuelan Equine Encephalitis Virus Vaccine Candidate</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Charles, Peter C ; Brown, Kevin W ; Davis, Nancy L ; Hart, Mary Kate ; Johnston, Robert E</creator><creatorcontrib>Charles, Peter C ; Brown, Kevin W ; Davis, Nancy L ; Hart, Mary Kate ; Johnston, Robert E</creatorcontrib><description>Induction of a mucosal immune response is generally thought to require introduction of an immunogen directly onto the mucosal surface. It has been observed, however, that live, attenuated mutants of the alphavirus, Venezuelan equine encephalitis virus (VEE), induce protection from virulent challenge at the respiratory mucosa even after parenteral inoculation. In this report, we propose a mechanism by which subcutaneous immunization with a molecularly cloned, attenuated double mutant of VEE is able to stimulate the production of mucosal anti-VEE IgA. Our results showed that the immunizing virus spread to, and replicated within, lymphoid tissues throughout the mouse. Several tissues known to be inductive sites of the mucosal immune system were found to be positive for the presence of VEE RNA by 48 hr postimmunization. Moreover, this mucosal lymphotropism resulted in the production of virus-specific IgA antibody detectable in vaginal secretions of immunized mice.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1996.8381</identifier><identifier>PMID: 9123821</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Administration, Intranasal ; Animals ; Antibodies, Viral - analysis ; Cell Line ; Cricetinae ; Encephalitis Virus, Venezuelan Equine - immunology ; Encephalitis Virus, Venezuelan Equine - metabolism ; Encephalitis Virus, Venezuelan Equine - physiology ; Encephalomyelitis, Venezuelan Equine - prevention & control ; Female ; IMMUNITE ; Immunity, Mucosal ; INMUNIDAD ; Lymphoid Tissue - immunology ; Mice ; RNA, Viral - analysis ; VACCIN ; Vaccination ; Vaccines, Attenuated - immunology ; VACUNA ; Viral Vaccines - immunology ; VIRUS DE LA ENCEFALOMIELITIS EQUINA ; VIRUS ENCEPHALOMYELITE EQUINE ; Virus Replication</subject><ispartof>Virology (New York, N.Y.), 1997-02, Vol.228 (2), p.153-160</ispartof><rights>1997 Academic Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-f3f21ab6dfcd083e3eb1a0214190297ec4bffef399f2611b8cf17abd25f7ca3</citedby><cites>FETCH-LOGICAL-c429t-f3f21ab6dfcd083e3eb1a0214190297ec4bffef399f2611b8cf17abd25f7ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682296983816$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9123821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Charles, Peter C</creatorcontrib><creatorcontrib>Brown, Kevin W</creatorcontrib><creatorcontrib>Davis, Nancy L</creatorcontrib><creatorcontrib>Hart, Mary Kate</creatorcontrib><creatorcontrib>Johnston, Robert E</creatorcontrib><title>Mucosal Immunity Induced by Parenteral Immunization with a Live Attenuated Venezuelan Equine Encephalitis Virus Vaccine Candidate</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Induction of a mucosal immune response is generally thought to require introduction of an immunogen directly onto the mucosal surface. It has been observed, however, that live, attenuated mutants of the alphavirus, Venezuelan equine encephalitis virus (VEE), induce protection from virulent challenge at the respiratory mucosa even after parenteral inoculation. In this report, we propose a mechanism by which subcutaneous immunization with a molecularly cloned, attenuated double mutant of VEE is able to stimulate the production of mucosal anti-VEE IgA. Our results showed that the immunizing virus spread to, and replicated within, lymphoid tissues throughout the mouse. Several tissues known to be inductive sites of the mucosal immune system were found to be positive for the presence of VEE RNA by 48 hr postimmunization. Moreover, this mucosal lymphotropism resulted in the production of virus-specific IgA antibody detectable in vaginal secretions of immunized mice.</description><subject>Administration, Intranasal</subject><subject>Animals</subject><subject>Antibodies, Viral - analysis</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>Encephalitis Virus, Venezuelan Equine - immunology</subject><subject>Encephalitis Virus, Venezuelan Equine - metabolism</subject><subject>Encephalitis Virus, Venezuelan Equine - physiology</subject><subject>Encephalomyelitis, Venezuelan Equine - prevention & control</subject><subject>Female</subject><subject>IMMUNITE</subject><subject>Immunity, Mucosal</subject><subject>INMUNIDAD</subject><subject>Lymphoid Tissue - immunology</subject><subject>Mice</subject><subject>RNA, Viral - analysis</subject><subject>VACCIN</subject><subject>Vaccination</subject><subject>Vaccines, Attenuated - immunology</subject><subject>VACUNA</subject><subject>Viral Vaccines - immunology</subject><subject>VIRUS DE LA ENCEFALOMIELITIS EQUINA</subject><subject>VIRUS ENCEPHALOMYELITE EQUINE</subject><subject>Virus Replication</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEcxYModVu9ehCEnLzNmmRmJ8mxLKtdWFGo9hoymW9sZCazzY8t25v_uRl26U28JITPe-8bvg-hd5QsKSHtp4ML05JK2S5FLegLtKBEthWpG_oSLQhpWNUKxl6jyxh_k_LmnFygC0lZLRhdoD9fs5miHvB2HLN36Yi3vs8Getwd8XcdwCcIz_hJJzd5_OjSPdZ45w6Ar1MCn3Uqjjvw8JRh0B5vHrLzgDfewP5eDy65iO9cyOXUxsxorX3v-uJ7g15ZPUR4e76v0O3nzY_1TbX79mW7vt5VpmEyVba2jOqu7a3piaihho5qwmhDJWGSg2k6a8HWUlrWUtoJYynXXc9WlhtdX6GPp9R9mB4yxKRGFw0M5bMw5ai4EJzQlfyvkLZM8BWbhcuT0IQpxgBW7YMbdTgqStRcjZqrUXM1aq6mGD6ck3M3Qv8sP3dR-PsTt3pS-ldwUf28lbwuCbxAcYJQVnRwEFQ0Dsp6exfAJNVP7l9z_wLvQqjv</recordid><startdate>19970217</startdate><enddate>19970217</enddate><creator>Charles, Peter C</creator><creator>Brown, Kevin W</creator><creator>Davis, Nancy L</creator><creator>Hart, Mary Kate</creator><creator>Johnston, Robert E</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970217</creationdate><title>Mucosal Immunity Induced by Parenteral Immunization with a Live Attenuated Venezuelan Equine Encephalitis Virus Vaccine Candidate</title><author>Charles, Peter C ; Brown, Kevin W ; Davis, Nancy L ; Hart, Mary Kate ; Johnston, Robert E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-f3f21ab6dfcd083e3eb1a0214190297ec4bffef399f2611b8cf17abd25f7ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Administration, Intranasal</topic><topic>Animals</topic><topic>Antibodies, Viral - analysis</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>Encephalitis Virus, Venezuelan Equine - immunology</topic><topic>Encephalitis Virus, Venezuelan Equine - metabolism</topic><topic>Encephalitis Virus, Venezuelan Equine - physiology</topic><topic>Encephalomyelitis, Venezuelan Equine - prevention & control</topic><topic>Female</topic><topic>IMMUNITE</topic><topic>Immunity, Mucosal</topic><topic>INMUNIDAD</topic><topic>Lymphoid Tissue - immunology</topic><topic>Mice</topic><topic>RNA, Viral - analysis</topic><topic>VACCIN</topic><topic>Vaccination</topic><topic>Vaccines, Attenuated - immunology</topic><topic>VACUNA</topic><topic>Viral Vaccines - immunology</topic><topic>VIRUS DE LA ENCEFALOMIELITIS EQUINA</topic><topic>VIRUS ENCEPHALOMYELITE EQUINE</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Charles, Peter C</creatorcontrib><creatorcontrib>Brown, Kevin W</creatorcontrib><creatorcontrib>Davis, Nancy L</creatorcontrib><creatorcontrib>Hart, Mary Kate</creatorcontrib><creatorcontrib>Johnston, Robert E</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Charles, Peter C</au><au>Brown, Kevin W</au><au>Davis, Nancy L</au><au>Hart, Mary Kate</au><au>Johnston, Robert E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mucosal Immunity Induced by Parenteral Immunization with a Live Attenuated Venezuelan Equine Encephalitis Virus Vaccine Candidate</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1997-02-17</date><risdate>1997</risdate><volume>228</volume><issue>2</issue><spage>153</spage><epage>160</epage><pages>153-160</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Induction of a mucosal immune response is generally thought to require introduction of an immunogen directly onto the mucosal surface. It has been observed, however, that live, attenuated mutants of the alphavirus, Venezuelan equine encephalitis virus (VEE), induce protection from virulent challenge at the respiratory mucosa even after parenteral inoculation. In this report, we propose a mechanism by which subcutaneous immunization with a molecularly cloned, attenuated double mutant of VEE is able to stimulate the production of mucosal anti-VEE IgA. Our results showed that the immunizing virus spread to, and replicated within, lymphoid tissues throughout the mouse. Several tissues known to be inductive sites of the mucosal immune system were found to be positive for the presence of VEE RNA by 48 hr postimmunization. Moreover, this mucosal lymphotropism resulted in the production of virus-specific IgA antibody detectable in vaginal secretions of immunized mice.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9123821</pmid><doi>10.1006/viro.1996.8381</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1997-02, Vol.228 (2), p.153-160 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78870159 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Administration, Intranasal Animals Antibodies, Viral - analysis Cell Line Cricetinae Encephalitis Virus, Venezuelan Equine - immunology Encephalitis Virus, Venezuelan Equine - metabolism Encephalitis Virus, Venezuelan Equine - physiology Encephalomyelitis, Venezuelan Equine - prevention & control Female IMMUNITE Immunity, Mucosal INMUNIDAD Lymphoid Tissue - immunology Mice RNA, Viral - analysis VACCIN Vaccination Vaccines, Attenuated - immunology VACUNA Viral Vaccines - immunology VIRUS DE LA ENCEFALOMIELITIS EQUINA VIRUS ENCEPHALOMYELITE EQUINE Virus Replication |
| title | Mucosal Immunity Induced by Parenteral Immunization with a Live Attenuated Venezuelan Equine Encephalitis Virus Vaccine Candidate |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T10%3A46%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mucosal%20Immunity%20Induced%20by%20Parenteral%20Immunization%20with%20a%20Live%20Attenuated%20Venezuelan%20Equine%20Encephalitis%20Virus%20Vaccine%20Candidate&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Charles,%20Peter%20C&rft.date=1997-02-17&rft.volume=228&rft.issue=2&rft.spage=153&rft.epage=160&rft.pages=153-160&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1006/viro.1996.8381&rft_dat=%3Cproquest_cross%3E16287529%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16287529&rft_id=info:pmid/9123821&rft_els_id=S0042682296983816&rfr_iscdi=true |