Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme
We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (h...
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Veröffentlicht in: | Current microbiology 1997-04, Vol.34 (4), p.258-263 |
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description | We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. A 2.8-kb DNA fragment containing the 1.3-kb hemA gene of Chlorobium was cloned into a hemC auxotroph (Sz16) of Bacillus subtilis, and complementation of the auxotroph to prototrophy was achieved. DNA sequence data showed a single open reading frame of 840 bp coding a protein of 279 amino acid residues. The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences. |
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H</creator><creatorcontrib>MAJUMDAR, D ; WYCHE, J. H</creatorcontrib><description>We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. A 2.8-kb DNA fragment containing the 1.3-kb hemA gene of Chlorobium was cloned into a hemC auxotroph (Sz16) of Bacillus subtilis, and complementation of the auxotroph to prototrophy was achieved. DNA sequence data showed a single open reading frame of 840 bp coding a protein of 279 amino acid residues. The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849900179</identifier><identifier>PMID: 9058548</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Amino Acid Sequence ; Amino acids ; Bacillus subtilis - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chlorobi - genetics ; Chlorobium vibrioforme ; Cloning ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; E coli ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes ; Genetic Complementation Test ; Genetics ; Hydroxymethylbilane Synthase - genetics ; Hydroxymethylbilane Synthase - metabolism ; Microbiology ; Molecular Sequence Data ; Open Reading Frames ; Restriction Mapping ; Sequence Homology, Amino Acid ; Space life sciences ; Transformation, Genetic</subject><ispartof>Current microbiology, 1997-04, Vol.34 (4), p.258-263</ispartof><rights>1997 INIST-CNRS</rights><rights>Springer-Verlag New York Inc. 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2625440$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9058548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MAJUMDAR, D</creatorcontrib><creatorcontrib>WYCHE, J. H</creatorcontrib><title>Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. A 2.8-kb DNA fragment containing the 1.3-kb hemA gene of Chlorobium was cloned into a hemC auxotroph (Sz16) of Bacillus subtilis, and complementation of the auxotroph to prototrophy was achieved. DNA sequence data showed a single open reading frame of 840 bp coding a protein of 279 amino acid residues. The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chlorobi - genetics</subject><subject>Chlorobium vibrioforme</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Hydroxymethylbilane Synthase - genetics</subject><subject>Hydroxymethylbilane Synthase - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Amino Acid</subject><subject>Space life sciences</subject><subject>Transformation, Genetic</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0U1rGzEQBmARUlLXybHHgiChJ2-iz9XqWEzaFBxySc-LVpqNFbSSI3kL_fdViTE0l5yGYR5eGF6EPlNyTQlRN4UQ1gmtCaFKn6AFFZw1RGt6ihaEC950raQf0adSnithmtAzdKaJ7KToFijfpwB2DiZjG1L08Qmb6HCcbYC09w5wgZcZogWcRrzfAt6lvNumwQcf0xNE7MBMPpoCuG6wwluY1is85jTh9TakXOk84d9-yD6NKU9wjj6MJhS4OMwl-vX99nF912wefvxcf9s0lnO2bxgIAsqpobPGUbB8sJKP3GkiOJWjswCSCesY14oNreDODmCUbKUxFbd8ib6-5u5yqi-UfT_5YiEEEyHNpVdd12rR8nchlbpVgtEKL9_A5zTnWJ_oqWCKC8koqap5VTanUjKM_S77yeQ_PSX9v8r6_yqr_sshdR4mcEd96Kjerw53U6wJYzbR-nJkrGVSCML_AtWYnu4</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>MAJUMDAR, D</creator><creator>WYCHE, J. 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H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-2e40e7d7b8cad1ec3bc53f3d904315fdcee524cd23972b643dcbea7565aac3b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chlorobi - genetics</topic><topic>Chlorobium vibrioforme</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Hydroxymethylbilane Synthase - genetics</topic><topic>Hydroxymethylbilane Synthase - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Open Reading Frames</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Amino Acid</topic><topic>Space life sciences</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAJUMDAR, D</creatorcontrib><creatorcontrib>WYCHE, J. 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H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>34</volume><issue>4</issue><spage>258</spage><epage>263</epage><pages>258-263</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. A 2.8-kb DNA fragment containing the 1.3-kb hemA gene of Chlorobium was cloned into a hemC auxotroph (Sz16) of Bacillus subtilis, and complementation of the auxotroph to prototrophy was achieved. DNA sequence data showed a single open reading frame of 840 bp coding a protein of 279 amino acid residues. The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>9058548</pmid><doi>10.1007/s002849900179</doi><tpages>6</tpages></addata></record> |
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subjects | Amino Acid Sequence Amino acids Bacillus subtilis - genetics Bacteriology Base Sequence Biological and medical sciences Biotechnology Chlorobi - genetics Chlorobium vibrioforme Cloning Cloning, Molecular Deoxyribonucleic acid DNA E coli Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes Genetic Complementation Test Genetics Hydroxymethylbilane Synthase - genetics Hydroxymethylbilane Synthase - metabolism Microbiology Molecular Sequence Data Open Reading Frames Restriction Mapping Sequence Homology, Amino Acid Space life sciences Transformation, Genetic |
title | Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme |
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