Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme

We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (h...

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Veröffentlicht in:Current microbiology 1997-04, Vol.34 (4), p.258-263
Hauptverfasser: MAJUMDAR, D, WYCHE, J. H
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description We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. A 2.8-kb DNA fragment containing the 1.3-kb hemA gene of Chlorobium was cloned into a hemC auxotroph (Sz16) of Bacillus subtilis, and complementation of the auxotroph to prototrophy was achieved. DNA sequence data showed a single open reading frame of 840 bp coding a protein of 279 amino acid residues. The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences.
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The deduced amino acid sequence of the Chlorobium porphobilinogen deaminase revealed 39% to 46% homology with the corresponding prokaryotic and eukaryotic sequences.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849900179</identifier><identifier>PMID: 9058548</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Amino Acid Sequence ; Amino acids ; Bacillus subtilis - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chlorobi - genetics ; Chlorobium vibrioforme ; Cloning ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; E coli ; Fundamental and applied biological sciences. 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H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>34</volume><issue>4</issue><spage>258</spage><epage>263</epage><pages>258-263</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>We previously reported the DNA sequence and expression of the Chlorobium vibrioforme glutamyl-tRNA reductase (hemA) gene (Majumdar et al., Arch Microbiol 156:281, 1991). The sequence downstream of the hemA gene indicated homology to Escherichia coli and Bacillus subtilis porphobilinogen deaminase (hemC) gene. The Chlorobium gene was confirmed to be the porphobilinogen deaminase gene, and complete sequence of the structural gene was obtained. 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subjects Amino Acid Sequence
Amino acids
Bacillus subtilis - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Biotechnology
Chlorobi - genetics
Chlorobium vibrioforme
Cloning
Cloning, Molecular
Deoxyribonucleic acid
DNA
E coli
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes
Genetic Complementation Test
Genetics
Hydroxymethylbilane Synthase - genetics
Hydroxymethylbilane Synthase - metabolism
Microbiology
Molecular Sequence Data
Open Reading Frames
Restriction Mapping
Sequence Homology, Amino Acid
Space life sciences
Transformation, Genetic
title Molecular cloning and nucleotide sequence of the porphobilinogen deaminase gene, hemC, from Chlorobium vibrioforme
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