Activity of type 1 5α-reductase is greater in the follicular infrainfundibulum compared with the epidermis
Summary The enzyme 5α–reductase converts testosterone (T) to dihydrotestosterone (DHT). Although this enzyme has been localized to various regions of the pilosebaceous unit, its activity has not been studied in the follicular portion of either vellus or sebaceous follicles. The goal of our study was...
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| Veröffentlicht in: | British journal of dermatology (1951) 1997-02, Vol.136 (2), p.166-171 |
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| container_title | British journal of dermatology (1951) |
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| creator | THIBOUTOT, D.M. KNAGGS, H. GILLILAND, K. HAGARI, S. |
| description | Summary
The enzyme 5α–reductase converts testosterone (T) to dihydrotestosterone (DHT). Although this enzyme has been localized to various regions of the pilosebaceous unit, its activity has not been studied in the follicular portion of either vellus or sebaceous follicles. The goal of our study was to determine the relative activities of 5α–reductase within various regions of these follicles with particular emphasis on the infrainfundibulum. A finding of increased 5α–reductase activity in upper follicles compared to epidermis might support the hypothesis that increased follicular production of DHT is involved in the hyperkeratinization observed in this region of the follicle in acne vulgaris. 5α–reductase activity was determined at pH 5 (optimal for the type 2 isozyme) and pH 7 (optimal for the type 1 isozyme) in isolated infrainfundibular segments from sebaceous and vellus follicles, homogenized epidermis from various anatomical areas and in microdissected segments of the pilosebaceous unit from breast skin of normal subjects. Enzyme activity was also determined at pH 7 in cultured infrainfundibular keratinocytes and in interfollicular epidermal keratinocytes. Homogenates of infrainfundibular segments demonstrated significantly greater activity at pH 7 compared to pH 5 (P < 0.001), confirming activity of the type 1 5α–reductase in this region. Activity of 5α–reductase was much lower in homogenized epidermis and did not demonstrate a clear pH preference. Keratinocytes cultured from the infrainfundibulum demonstrated significantly greater 5α–reductase activity compared to keratinocytes from interfollicular epidermis (P = 0.04). In the dissected segments of pilosebaceous units from breast skin, 5α–reductase activity was greatest in the sebaceous gland followed by the sebaceous duct, infrainfundibulum, whole skin and epidermis. These data indicate that 5α–reductase activity varies within regions of the pilosebaceous unit and compared with interfollicular epidermal cells, infrainfundibular keratinocytes have an increased capacity for producing androgens which may play a role in the follicular hyperkeratinization seen in acne. |
| doi_str_mv | 10.1046/j.1365-2133.1997.d01-1162.x |
| format | Article |
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The enzyme 5α–reductase converts testosterone (T) to dihydrotestosterone (DHT). Although this enzyme has been localized to various regions of the pilosebaceous unit, its activity has not been studied in the follicular portion of either vellus or sebaceous follicles. The goal of our study was to determine the relative activities of 5α–reductase within various regions of these follicles with particular emphasis on the infrainfundibulum. A finding of increased 5α–reductase activity in upper follicles compared to epidermis might support the hypothesis that increased follicular production of DHT is involved in the hyperkeratinization observed in this region of the follicle in acne vulgaris. 5α–reductase activity was determined at pH 5 (optimal for the type 2 isozyme) and pH 7 (optimal for the type 1 isozyme) in isolated infrainfundibular segments from sebaceous and vellus follicles, homogenized epidermis from various anatomical areas and in microdissected segments of the pilosebaceous unit from breast skin of normal subjects. Enzyme activity was also determined at pH 7 in cultured infrainfundibular keratinocytes and in interfollicular epidermal keratinocytes. Homogenates of infrainfundibular segments demonstrated significantly greater activity at pH 7 compared to pH 5 (P < 0.001), confirming activity of the type 1 5α–reductase in this region. Activity of 5α–reductase was much lower in homogenized epidermis and did not demonstrate a clear pH preference. Keratinocytes cultured from the infrainfundibulum demonstrated significantly greater 5α–reductase activity compared to keratinocytes from interfollicular epidermis (P = 0.04). In the dissected segments of pilosebaceous units from breast skin, 5α–reductase activity was greatest in the sebaceous gland followed by the sebaceous duct, infrainfundibulum, whole skin and epidermis. These data indicate that 5α–reductase activity varies within regions of the pilosebaceous unit and compared with interfollicular epidermal cells, infrainfundibular keratinocytes have an increased capacity for producing androgens which may play a role in the follicular hyperkeratinization seen in acne.</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1046/j.1365-2133.1997.d01-1162.x</identifier><identifier>PMID: 9068725</identifier><identifier>CODEN: BJDEAZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Breast ; Cell Culture Techniques ; Cholestenone 5 alpha-Reductase ; Dermatology ; Epidermis - enzymology ; Face ; Female ; Hair Follicle - enzymology ; Humans ; Keratinocytes - enzymology ; Male ; Medical sciences ; Middle Aged ; Oxidoreductases - metabolism ; Sebaceous Glands - enzymology ; Skin involvement in other diseases. Miscellaneous. General aspects</subject><ispartof>British journal of dermatology (1951), 1997-02, Vol.136 (2), p.166-171</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3716-eb05a4c60812d4e0d9db69516ff344382ffe35679116c8f285f3e91bb6a0c1c43</citedby><cites>FETCH-LOGICAL-c3716-eb05a4c60812d4e0d9db69516ff344382ffe35679116c8f285f3e91bb6a0c1c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2133.1997.d01-1162.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2133.1997.d01-1162.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,1411,23909,23910,25118,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2585043$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9068725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>THIBOUTOT, D.M.</creatorcontrib><creatorcontrib>KNAGGS, H.</creatorcontrib><creatorcontrib>GILLILAND, K.</creatorcontrib><creatorcontrib>HAGARI, S.</creatorcontrib><title>Activity of type 1 5α-reductase is greater in the follicular infrainfundibulum compared with the epidermis</title><title>British journal of dermatology (1951)</title><addtitle>Br J Dermatol</addtitle><description>Summary
The enzyme 5α–reductase converts testosterone (T) to dihydrotestosterone (DHT). Although this enzyme has been localized to various regions of the pilosebaceous unit, its activity has not been studied in the follicular portion of either vellus or sebaceous follicles. The goal of our study was to determine the relative activities of 5α–reductase within various regions of these follicles with particular emphasis on the infrainfundibulum. A finding of increased 5α–reductase activity in upper follicles compared to epidermis might support the hypothesis that increased follicular production of DHT is involved in the hyperkeratinization observed in this region of the follicle in acne vulgaris. 5α–reductase activity was determined at pH 5 (optimal for the type 2 isozyme) and pH 7 (optimal for the type 1 isozyme) in isolated infrainfundibular segments from sebaceous and vellus follicles, homogenized epidermis from various anatomical areas and in microdissected segments of the pilosebaceous unit from breast skin of normal subjects. Enzyme activity was also determined at pH 7 in cultured infrainfundibular keratinocytes and in interfollicular epidermal keratinocytes. Homogenates of infrainfundibular segments demonstrated significantly greater activity at pH 7 compared to pH 5 (P < 0.001), confirming activity of the type 1 5α–reductase in this region. Activity of 5α–reductase was much lower in homogenized epidermis and did not demonstrate a clear pH preference. Keratinocytes cultured from the infrainfundibulum demonstrated significantly greater 5α–reductase activity compared to keratinocytes from interfollicular epidermis (P = 0.04). In the dissected segments of pilosebaceous units from breast skin, 5α–reductase activity was greatest in the sebaceous gland followed by the sebaceous duct, infrainfundibulum, whole skin and epidermis. These data indicate that 5α–reductase activity varies within regions of the pilosebaceous unit and compared with interfollicular epidermal cells, infrainfundibular keratinocytes have an increased capacity for producing androgens which may play a role in the follicular hyperkeratinization seen in acne.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Breast</subject><subject>Cell Culture Techniques</subject><subject>Cholestenone 5 alpha-Reductase</subject><subject>Dermatology</subject><subject>Epidermis - enzymology</subject><subject>Face</subject><subject>Female</subject><subject>Hair Follicle - enzymology</subject><subject>Humans</subject><subject>Keratinocytes - enzymology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Oxidoreductases - metabolism</subject><subject>Sebaceous Glands - enzymology</subject><subject>Skin involvement in other diseases. Miscellaneous. General aspects</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtu1DAUhi1EVYbCIyBZArFLsOPYicUC9QLTlgIbEBIby3GOqafJJNgOnXksXoRnwumMZs_GlvxfzvGH0EtKckpK8WaVUyZ4VlDGcipllbeEZpSKIt88QouD9hgtCCFVRqRgT9DTEFaEUEY4OUbHkoi6KvgC3Z2a6H67uMWDxXE7AqaY__2TeWgnE3UA7AL-6UFH8NitcbwFbIeuc2bq9PxivU7HtG5dM3VTj83Qjzql8b2Ltw92GF0LvnfhGTqyugvwfH-foG8f3n89v8xuviyvzk9vMsMqKjJoCNelEaSmRVsCaWXbCMmpsJaVJasLa4FxUcn0ZVPbouaWgaRNIzQx1JTsBL3e9Y5--DVBiCoNN9B1eg3DFFRV10LwSibj253R-CEED1aN3vXabxUlakatVmrGqWacakatEmo1o1ablH6xHzM1PbSH7J5t0l_tdR2M7hKotXHhYCt4zUnJku3dznbvOtj-zwbq7PqCCpEasl2DCxE2hwbt75SoWMXV989L9eny4qNcSqZ-sH_f5auX</recordid><startdate>199702</startdate><enddate>199702</enddate><creator>THIBOUTOT, D.M.</creator><creator>KNAGGS, H.</creator><creator>GILLILAND, K.</creator><creator>HAGARI, S.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199702</creationdate><title>Activity of type 1 5α-reductase is greater in the follicular infrainfundibulum compared with the epidermis</title><author>THIBOUTOT, D.M. ; KNAGGS, H. ; GILLILAND, K. ; HAGARI, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3716-eb05a4c60812d4e0d9db69516ff344382ffe35679116c8f285f3e91bb6a0c1c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Breast</topic><topic>Cell Culture Techniques</topic><topic>Cholestenone 5 alpha-Reductase</topic><topic>Dermatology</topic><topic>Epidermis - enzymology</topic><topic>Face</topic><topic>Female</topic><topic>Hair Follicle - enzymology</topic><topic>Humans</topic><topic>Keratinocytes - enzymology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Oxidoreductases - metabolism</topic><topic>Sebaceous Glands - enzymology</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THIBOUTOT, D.M.</creatorcontrib><creatorcontrib>KNAGGS, H.</creatorcontrib><creatorcontrib>GILLILAND, K.</creatorcontrib><creatorcontrib>HAGARI, S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>THIBOUTOT, D.M.</au><au>KNAGGS, H.</au><au>GILLILAND, K.</au><au>HAGARI, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activity of type 1 5α-reductase is greater in the follicular infrainfundibulum compared with the epidermis</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>1997-02</date><risdate>1997</risdate><volume>136</volume><issue>2</issue><spage>166</spage><epage>171</epage><pages>166-171</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><coden>BJDEAZ</coden><abstract>Summary
The enzyme 5α–reductase converts testosterone (T) to dihydrotestosterone (DHT). Although this enzyme has been localized to various regions of the pilosebaceous unit, its activity has not been studied in the follicular portion of either vellus or sebaceous follicles. The goal of our study was to determine the relative activities of 5α–reductase within various regions of these follicles with particular emphasis on the infrainfundibulum. A finding of increased 5α–reductase activity in upper follicles compared to epidermis might support the hypothesis that increased follicular production of DHT is involved in the hyperkeratinization observed in this region of the follicle in acne vulgaris. 5α–reductase activity was determined at pH 5 (optimal for the type 2 isozyme) and pH 7 (optimal for the type 1 isozyme) in isolated infrainfundibular segments from sebaceous and vellus follicles, homogenized epidermis from various anatomical areas and in microdissected segments of the pilosebaceous unit from breast skin of normal subjects. Enzyme activity was also determined at pH 7 in cultured infrainfundibular keratinocytes and in interfollicular epidermal keratinocytes. Homogenates of infrainfundibular segments demonstrated significantly greater activity at pH 7 compared to pH 5 (P < 0.001), confirming activity of the type 1 5α–reductase in this region. Activity of 5α–reductase was much lower in homogenized epidermis and did not demonstrate a clear pH preference. Keratinocytes cultured from the infrainfundibulum demonstrated significantly greater 5α–reductase activity compared to keratinocytes from interfollicular epidermis (P = 0.04). In the dissected segments of pilosebaceous units from breast skin, 5α–reductase activity was greatest in the sebaceous gland followed by the sebaceous duct, infrainfundibulum, whole skin and epidermis. These data indicate that 5α–reductase activity varies within regions of the pilosebaceous unit and compared with interfollicular epidermal cells, infrainfundibular keratinocytes have an increased capacity for producing androgens which may play a role in the follicular hyperkeratinization seen in acne.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9068725</pmid><doi>10.1046/j.1365-2133.1997.d01-1162.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | British journal of dermatology (1951), 1997-02, Vol.136 (2), p.166-171 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescent Adult Aged Aged, 80 and over Biological and medical sciences Breast Cell Culture Techniques Cholestenone 5 alpha-Reductase Dermatology Epidermis - enzymology Face Female Hair Follicle - enzymology Humans Keratinocytes - enzymology Male Medical sciences Middle Aged Oxidoreductases - metabolism Sebaceous Glands - enzymology Skin involvement in other diseases. Miscellaneous. General aspects |
| title | Activity of type 1 5α-reductase is greater in the follicular infrainfundibulum compared with the epidermis |
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