Phase variation of gonococcal protein II: Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence
Expression of outer membrane protein II (P.II) of Neisseria gonorrhoeae is subject to reversible phase variation at a rate of 10 −3–10 −4/cell/generation. The signal peptide coding regions of P.II genes contain variable numbers of tandem repeats of the sequence CTCTT. Changes in the number of CTCTT...
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| Veröffentlicht in: | Cell 1989-02, Vol.56 (4), p.539-547 |
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| creator | Murphy, George L. Connell, Terry D. Barritt, Diana S. Koomey, Michael Cannon, Janne G. |
| description | Expression of outer membrane protein II (P.II) of Neisseria gonorrhoeae is subject to reversible phase variation at a rate of 10
−3–10
−4/cell/generation. The signal peptide coding regions of P.II genes contain variable numbers of tandem repeats of the sequence CTCTT. Changes in the number of CTCTT units, leading to frameshifting within the gene, are responsible for changes in P.II expression. Phase variation mediated by the CTCTT repeat also occurred in E. coli, as assayed with a P.II-alkaline phosphatase (
phoA) gene fusion. Phase variation in both the gonococcus and E. coli was
recA-independent, occurred at similar rates, and involved insertions or deletions of one or more repeat units. The characteristics of the phase variation process were consistent with a model in which expression of P.II genes is regulated by slipped-strand mispairing of the DNA in the CTCTT repeat region. |
| doi_str_mv | 10.1016/0092-8674(89)90577-1 |
| format | Article |
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−3–10
−4/cell/generation. The signal peptide coding regions of P.II genes contain variable numbers of tandem repeats of the sequence CTCTT. Changes in the number of CTCTT units, leading to frameshifting within the gene, are responsible for changes in P.II expression. Phase variation mediated by the CTCTT repeat also occurred in E. coli, as assayed with a P.II-alkaline phosphatase (
phoA) gene fusion. Phase variation in both the gonococcus and E. coli was
recA-independent, occurred at similar rates, and involved insertions or deletions of one or more repeat units. The characteristics of the phase variation process were consistent with a model in which expression of P.II genes is regulated by slipped-strand mispairing of the DNA in the CTCTT repeat region.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(89)90577-1</identifier><identifier>PMID: 2492905</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Bacterial Outer Membrane Proteins - genetics ; Biological and medical sciences ; Blotting, Southern ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation ; Genes, Bacterial ; Hydrogen Bonding ; Molecular and cellular biology ; Molecular genetics ; Neisseria gonorrhoeae ; Neisseria gonorrhoeae - genetics ; Rec A Recombinases - physiology ; Regulatory Sequences, Nucleic Acid ; Repetitive Sequences, Nucleic Acid</subject><ispartof>Cell, 1989-02, Vol.56 (4), p.539-547</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-f6f879bd3cd88fc69e253785f9fe19b3c38e883e58237d0e8502e97dea17bfcc3</citedby><cites>FETCH-LOGICAL-c463t-f6f879bd3cd88fc69e253785f9fe19b3c38e883e58237d0e8502e97dea17bfcc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0092867489905771$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6898692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2492905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murphy, George L.</creatorcontrib><creatorcontrib>Connell, Terry D.</creatorcontrib><creatorcontrib>Barritt, Diana S.</creatorcontrib><creatorcontrib>Koomey, Michael</creatorcontrib><creatorcontrib>Cannon, Janne G.</creatorcontrib><title>Phase variation of gonococcal protein II: Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence</title><title>Cell</title><addtitle>Cell</addtitle><description>Expression of outer membrane protein II (P.II) of Neisseria gonorrhoeae is subject to reversible phase variation at a rate of 10
−3–10
−4/cell/generation. The signal peptide coding regions of P.II genes contain variable numbers of tandem repeats of the sequence CTCTT. Changes in the number of CTCTT units, leading to frameshifting within the gene, are responsible for changes in P.II expression. Phase variation mediated by the CTCTT repeat also occurred in E. coli, as assayed with a P.II-alkaline phosphatase (
phoA) gene fusion. Phase variation in both the gonococcus and E. coli was
recA-independent, occurred at similar rates, and involved insertions or deletions of one or more repeat units. The characteristics of the phase variation process were consistent with a model in which expression of P.II genes is regulated by slipped-strand mispairing of the DNA in the CTCTT repeat region.</description><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes, Bacterial</subject><subject>Hydrogen Bonding</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Neisseria gonorrhoeae</subject><subject>Neisseria gonorrhoeae - genetics</subject><subject>Rec A Recombinases - physiology</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Repetitive Sequences, Nucleic Acid</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUha0KVKal_6BIXiAEixQ7iV9dIFUtj5EqQIiuLce-HowycWpnRnTZf47DjIYdrCz5fuc-zkHonJILSih_S4iqK8lF-1qqN4owISp6hBaUKFG1VNRP0OKAPEMnOf8khEjG2DE6rltVF8kCPX79YTLgrUnBTCEOOHq8ikO00VrT4zHFCcKAl8tL_A1Wm_4vBANg-DUmyHn-6h5w7sM4gqvylMzg8Drk0YQUhtXMG5xghClMYQv45vMVznC_gcHCc_TUmz7D2f49RXcf3n-__lTdfvm4vL66rWzLm6ny3EuhOtdYJ6W3XEHNGiGZVx6o6hrbSJCyASbrRjgCkpEalHBgqOi8tc0perXrW24qk_Oky4IW-t4MEDdZCyk5L_78F6SsTKZtXcB2B9oUc07g9ZjC2qQHTYmeI9Kz_3r2X0ul_0SkaZG92PffdGtwB9E-k1J_ua-bXDLwxUwb8gHjUkmu5unvdhgU07YBks42zIa6kMBO2sXw7z1-A3Iqrtk</recordid><startdate>19890224</startdate><enddate>19890224</enddate><creator>Murphy, George L.</creator><creator>Connell, Terry D.</creator><creator>Barritt, Diana S.</creator><creator>Koomey, Michael</creator><creator>Cannon, Janne G.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890224</creationdate><title>Phase variation of gonococcal protein II: Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence</title><author>Murphy, George L. ; Connell, Terry D. ; Barritt, Diana S. ; Koomey, Michael ; Cannon, Janne G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-f6f879bd3cd88fc69e253785f9fe19b3c38e883e58237d0e8502e97dea17bfcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes, Bacterial</topic><topic>Hydrogen Bonding</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Neisseria gonorrhoeae</topic><topic>Neisseria gonorrhoeae - genetics</topic><topic>Rec A Recombinases - physiology</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Repetitive Sequences, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murphy, George L.</creatorcontrib><creatorcontrib>Connell, Terry D.</creatorcontrib><creatorcontrib>Barritt, Diana S.</creatorcontrib><creatorcontrib>Koomey, Michael</creatorcontrib><creatorcontrib>Cannon, Janne G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murphy, George L.</au><au>Connell, Terry D.</au><au>Barritt, Diana S.</au><au>Koomey, Michael</au><au>Cannon, Janne G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phase variation of gonococcal protein II: Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1989-02-24</date><risdate>1989</risdate><volume>56</volume><issue>4</issue><spage>539</spage><epage>547</epage><pages>539-547</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>Expression of outer membrane protein II (P.II) of Neisseria gonorrhoeae is subject to reversible phase variation at a rate of 10
−3–10
−4/cell/generation. The signal peptide coding regions of P.II genes contain variable numbers of tandem repeats of the sequence CTCTT. Changes in the number of CTCTT units, leading to frameshifting within the gene, are responsible for changes in P.II expression. Phase variation mediated by the CTCTT repeat also occurred in E. coli, as assayed with a P.II-alkaline phosphatase (
phoA) gene fusion. Phase variation in both the gonococcus and E. coli was
recA-independent, occurred at similar rates, and involved insertions or deletions of one or more repeat units. The characteristics of the phase variation process were consistent with a model in which expression of P.II genes is regulated by slipped-strand mispairing of the DNA in the CTCTT repeat region.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>2492905</pmid><doi>10.1016/0092-8674(89)90577-1</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cell, 1989-02, Vol.56 (4), p.539-547 |
| issn | 0092-8674 1097-4172 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial Outer Membrane Proteins - genetics Biological and medical sciences Blotting, Southern DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation Genes, Bacterial Hydrogen Bonding Molecular and cellular biology Molecular genetics Neisseria gonorrhoeae Neisseria gonorrhoeae - genetics Rec A Recombinases - physiology Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid |
| title | Phase variation of gonococcal protein II: Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence |
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