CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner
Very few growth inhibitors have been identified which can inhibit the proliferation of a broad spectrum of human breast cancer cell lines. CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor, can reversibly inhibit the proliferation of both estrogen receptor positive (MCF-7) and negati...
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| Veröffentlicht in: | Breast cancer research and treatment 1997, Vol.42 (2), p.137-148 |
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| container_title | Breast cancer research and treatment |
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| creator | BETZ, N. A FATTAEY, H. k WESTHOFF, B. A PAULSEN, A. Q JOHNSON, T. C |
| description | Very few growth inhibitors have been identified which can inhibit the proliferation of a broad spectrum of human breast cancer cell lines. CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor, can reversibly inhibit the proliferation of both estrogen receptor positive (MCF-7) and negative (BT-20) human breast cancer cell lines. In addition, at concentrations above those required for the reversible inhibition of cell proliferation, CeReS-18 can also induce cell death in MCF-7 cells. Changes in nuclear and cytoplasmic morphology, characteristic of apoptosis, were detected in MCF-7 cells treated with a cytotoxic concentration of CeReS-18, and internucleosomal DNA cleavage was also observed. The sensitivity of MCF-7 and BT-20 cells to the biological properties of CeReS-18 could be influenced by altering the calcium concentration in the extracellular growth medium, such that when the calcium concentration in the environment was decreased, and increased sensitivity to CeReS-18-induced growth inhibition and cytotoxicity were observed. The addition of the calcium chelating agent EGTA to MCF-7 cells, cultured in a normal calcium environment, could mimic the increased sensitivity to the biological effects of CeReS-18 observed under reduced calcium conditions. |
| doi_str_mv | 10.1023/A:1005735723808 |
| format | Article |
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The sensitivity of MCF-7 and BT-20 cells to the biological properties of CeReS-18 could be influenced by altering the calcium concentration in the extracellular growth medium, such that when the calcium concentration in the environment was decreased, and increased sensitivity to CeReS-18-induced growth inhibition and cytotoxicity were observed. The addition of the calcium chelating agent EGTA to MCF-7 cells, cultured in a normal calcium environment, could mimic the increased sensitivity to the biological effects of CeReS-18 observed under reduced calcium conditions.</description><identifier>ISSN: 0167-6806</identifier><identifier>EISSN: 1573-7217</identifier><identifier>DOI: 10.1023/A:1005735723808</identifier><identifier>PMID: 9138603</identifier><identifier>CODEN: BCTRD6</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Antineoplastic Agents - pharmacology ; Apoptosis - drug effects ; Biological and medical sciences ; Breast cancer ; Breast Neoplasms - drug therapy ; Breast Neoplasms - pathology ; Breast Neoplasms - ultrastructure ; Calcium ; Calcium - metabolism ; Cancer research ; Cell Cycle - drug effects ; Cell Division - drug effects ; Drug Screening Assays, Antitumor ; Extracellular Space - metabolism ; Gynecology. Andrology. 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A</creatorcontrib><creatorcontrib>FATTAEY, H. k</creatorcontrib><creatorcontrib>WESTHOFF, B. A</creatorcontrib><creatorcontrib>PAULSEN, A. Q</creatorcontrib><creatorcontrib>JOHNSON, T. C</creatorcontrib><title>CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner</title><title>Breast cancer research and treatment</title><addtitle>Breast Cancer Res Treat</addtitle><description>Very few growth inhibitors have been identified which can inhibit the proliferation of a broad spectrum of human breast cancer cell lines. CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor, can reversibly inhibit the proliferation of both estrogen receptor positive (MCF-7) and negative (BT-20) human breast cancer cell lines. In addition, at concentrations above those required for the reversible inhibition of cell proliferation, CeReS-18 can also induce cell death in MCF-7 cells. Changes in nuclear and cytoplasmic morphology, characteristic of apoptosis, were detected in MCF-7 cells treated with a cytotoxic concentration of CeReS-18, and internucleosomal DNA cleavage was also observed. The sensitivity of MCF-7 and BT-20 cells to the biological properties of CeReS-18 could be influenced by altering the calcium concentration in the extracellular growth medium, such that when the calcium concentration in the environment was decreased, and increased sensitivity to CeReS-18-induced growth inhibition and cytotoxicity were observed. The addition of the calcium chelating agent EGTA to MCF-7 cells, cultured in a normal calcium environment, could mimic the increased sensitivity to the biological effects of CeReS-18 observed under reduced calcium conditions.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - ultrastructure</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Cancer research</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Extracellular Space - metabolism</subject><subject>Gynecology. Andrology. 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A ; FATTAEY, H. k ; WESTHOFF, B. A ; PAULSEN, A. Q ; JOHNSON, T. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-2da340291c8d1409af4d1ef4191b04e8d1a4e9640e7eda8764c04de9e662a10e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Breast Neoplasms - pathology</topic><topic>Breast Neoplasms - ultrastructure</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Cancer research</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Extracellular Space - metabolism</topic><topic>Gynecology. Andrology. 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A</au><au>FATTAEY, H. k</au><au>WESTHOFF, B. A</au><au>PAULSEN, A. Q</au><au>JOHNSON, T. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner</atitle><jtitle>Breast cancer research and treatment</jtitle><addtitle>Breast Cancer Res Treat</addtitle><date>1997</date><risdate>1997</risdate><volume>42</volume><issue>2</issue><spage>137</spage><epage>148</epage><pages>137-148</pages><issn>0167-6806</issn><eissn>1573-7217</eissn><coden>BCTRD6</coden><abstract>Very few growth inhibitors have been identified which can inhibit the proliferation of a broad spectrum of human breast cancer cell lines. CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor, can reversibly inhibit the proliferation of both estrogen receptor positive (MCF-7) and negative (BT-20) human breast cancer cell lines. In addition, at concentrations above those required for the reversible inhibition of cell proliferation, CeReS-18 can also induce cell death in MCF-7 cells. Changes in nuclear and cytoplasmic morphology, characteristic of apoptosis, were detected in MCF-7 cells treated with a cytotoxic concentration of CeReS-18, and internucleosomal DNA cleavage was also observed. The sensitivity of MCF-7 and BT-20 cells to the biological properties of CeReS-18 could be influenced by altering the calcium concentration in the extracellular growth medium, such that when the calcium concentration in the environment was decreased, and increased sensitivity to CeReS-18-induced growth inhibition and cytotoxicity were observed. The addition of the calcium chelating agent EGTA to MCF-7 cells, cultured in a normal calcium environment, could mimic the increased sensitivity to the biological effects of CeReS-18 observed under reduced calcium conditions.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>9138603</pmid><doi>10.1023/A:1005735723808</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0167-6806 |
| ispartof | Breast cancer research and treatment, 1997, Vol.42 (2), p.137-148 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Antineoplastic Agents - pharmacology Apoptosis - drug effects Biological and medical sciences Breast cancer Breast Neoplasms - drug therapy Breast Neoplasms - pathology Breast Neoplasms - ultrastructure Calcium Calcium - metabolism Cancer research Cell Cycle - drug effects Cell Division - drug effects Drug Screening Assays, Antitumor Extracellular Space - metabolism Gynecology. Andrology. Obstetrics Humans Mammary gland diseases Medical sciences Proteins Receptors, Estrogen - physiology Sialoglycoproteins - pharmacology Tumor Cells, Cultured Tumors |
| title | CeReS-18, a novel cell surface sialoglycopeptide, induces cell cycle arrest and apoptosis in a calcium-sensitive manner |
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