Radioimmunoassay of free β-subunit of human chorionic gonadotropin in diagnosis of high-risk and low-risk gestational trophoblastic disease
A radioimmunoassay was performed with monoclonal antibody 1E5, which distinguishes free beta-subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotrophin. Serum samples were obtained from 68 pregnant women, 9 with hydatidiform mole who underwent spontaneous remissi...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1989-02, Vol.160 (2), p.444-449 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | KHAZAELI, M. B BUCHINA, E. S PATTILLO, R. A SOONG, S.-J HATCH, K. D |
| description | A radioimmunoassay was performed with monoclonal antibody 1E5, which distinguishes free beta-subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotrophin. Serum samples were obtained from 68 pregnant women, 9 with hydatidiform mole who underwent spontaneous remission, 12 with hydatidiform mole who developed gestational trophoblastic disease, 5 with metastatic gestational trophoblastic disease of high-risk category, and 1 with choriocarcinoma concomitant with pregnancy. The concentrations of free beta-subunit of human chorionic gonadotropin and total beta-subunit were determined on the sera. The assay data were expressed as a ratio of nanograms of free beta-subunit per 1000 mIU of total beta-subunit. The ratios, analyzed by the Wilcoxon two-sample test, indicated a highly significant correlation between high ratios and the eventual diagnosis of high-risk gestational trophoblastic disease (p = 0.0019). This study suggests that the excessive production of free beta-subunit of human chorionic gonadotrophin may identify patients with high-risk gestational trophoblastic disease much earlier and identify gestational trophoblastic disease in patients during pregnancy. |
| doi_str_mv | 10.1016/0002-9378(89)90469-9 |
| format | Article |
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B ; BUCHINA, E. S ; PATTILLO, R. A ; SOONG, S.-J ; HATCH, K. D</creator><creatorcontrib>KHAZAELI, M. B ; BUCHINA, E. S ; PATTILLO, R. A ; SOONG, S.-J ; HATCH, K. D</creatorcontrib><description>A radioimmunoassay was performed with monoclonal antibody 1E5, which distinguishes free beta-subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotrophin. Serum samples were obtained from 68 pregnant women, 9 with hydatidiform mole who underwent spontaneous remission, 12 with hydatidiform mole who developed gestational trophoblastic disease, 5 with metastatic gestational trophoblastic disease of high-risk category, and 1 with choriocarcinoma concomitant with pregnancy. The concentrations of free beta-subunit of human chorionic gonadotropin and total beta-subunit were determined on the sera. The assay data were expressed as a ratio of nanograms of free beta-subunit per 1000 mIU of total beta-subunit. The ratios, analyzed by the Wilcoxon two-sample test, indicated a highly significant correlation between high ratios and the eventual diagnosis of high-risk gestational trophoblastic disease (p = 0.0019). This study suggests that the excessive production of free beta-subunit of human chorionic gonadotrophin may identify patients with high-risk gestational trophoblastic disease much earlier and identify gestational trophoblastic disease in patients during pregnancy.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/0002-9378(89)90469-9</identifier><identifier>PMID: 2464935</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Elsevier</publisher><subject>Adult ; Biological and medical sciences ; Chorionic Gonadotropin - blood ; Chorionic Gonadotropin, beta Subunit, Human ; Diseases of mother, fetus and pregnancy ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; Hydatidiform Mole ; Medical sciences ; Middle Aged ; Neoplasms, Multiple Primary ; Peptide Fragments - blood ; Pregnancy ; Pregnancy. Fetus. Placenta ; Radioimmunoassay ; Risk Factors ; Trophoblastic Neoplasms - diagnosis ; Uterine Neoplasms - diagnosis</subject><ispartof>American journal of obstetrics and gynecology, 1989-02, Vol.160 (2), p.444-449</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7368401$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2464935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KHAZAELI, M. B</creatorcontrib><creatorcontrib>BUCHINA, E. S</creatorcontrib><creatorcontrib>PATTILLO, R. A</creatorcontrib><creatorcontrib>SOONG, S.-J</creatorcontrib><creatorcontrib>HATCH, K. D</creatorcontrib><title>Radioimmunoassay of free β-subunit of human chorionic gonadotropin in diagnosis of high-risk and low-risk gestational trophoblastic disease</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>A radioimmunoassay was performed with monoclonal antibody 1E5, which distinguishes free beta-subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotrophin. Serum samples were obtained from 68 pregnant women, 9 with hydatidiform mole who underwent spontaneous remission, 12 with hydatidiform mole who developed gestational trophoblastic disease, 5 with metastatic gestational trophoblastic disease of high-risk category, and 1 with choriocarcinoma concomitant with pregnancy. The concentrations of free beta-subunit of human chorionic gonadotropin and total beta-subunit were determined on the sera. The assay data were expressed as a ratio of nanograms of free beta-subunit per 1000 mIU of total beta-subunit. The ratios, analyzed by the Wilcoxon two-sample test, indicated a highly significant correlation between high ratios and the eventual diagnosis of high-risk gestational trophoblastic disease (p = 0.0019). This study suggests that the excessive production of free beta-subunit of human chorionic gonadotrophin may identify patients with high-risk gestational trophoblastic disease much earlier and identify gestational trophoblastic disease in patients during pregnancy.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Chorionic Gonadotropin - blood</subject><subject>Chorionic Gonadotropin, beta Subunit, Human</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Hydatidiform Mole</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasms, Multiple Primary</subject><subject>Peptide Fragments - blood</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Radioimmunoassay</subject><subject>Risk Factors</subject><subject>Trophoblastic Neoplasms - diagnosis</subject><subject>Uterine Neoplasms - diagnosis</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kN1KHEEQhRuJ6GryBgb6Ioi5mKT_pqf7UkSNIARCvF5q-me3zUz3OjWD7DvkafIgPlNmdREKiqrznUNRhJxx9o0zrr8zxkRlZWMujP1qmdK2sgdkwZltKm20-UAW78gxOUF83I3CiiNyJJRWVtYL8vcX-FRS30-5ACJsaYk0DiHQl38VTu2U07hbraceMnXrMqSSk6OrksGXcSiblOlcPsEqF0z4CqfVuhoS_qGQPe3K89uwCjjCOPuhozvnurQd4Din-YQBMHwkhxE6DJ_2_ZQ83Fz_vvpR3f-8vbu6vK82QtZj1coYg2qiaWouayWkYkKbEHUroOZgvJLKK-e4r73WVopGW-0dd1waGxspT8n5W-5mKE_TfNWyT-hC10EOZcJlY0xtuWYz-HkPTm0f_HIzpB6G7XL_vln_stcBHXRxgOwSvmON1EYxLv8DjQ2Cpw</recordid><startdate>19890201</startdate><enddate>19890201</enddate><creator>KHAZAELI, M. B</creator><creator>BUCHINA, E. S</creator><creator>PATTILLO, R. A</creator><creator>SOONG, S.-J</creator><creator>HATCH, K. D</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19890201</creationdate><title>Radioimmunoassay of free β-subunit of human chorionic gonadotropin in diagnosis of high-risk and low-risk gestational trophoblastic disease</title><author>KHAZAELI, M. B ; BUCHINA, E. S ; PATTILLO, R. A ; SOONG, S.-J ; HATCH, K. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-b3ffe47f87513542340268ef6b2a51a8d434d4cc1d5d669327696dc1c1389f733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Chorionic Gonadotropin - blood</topic><topic>Chorionic Gonadotropin, beta Subunit, Human</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Hydatidiform Mole</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasms, Multiple Primary</topic><topic>Peptide Fragments - blood</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Radioimmunoassay</topic><topic>Risk Factors</topic><topic>Trophoblastic Neoplasms - diagnosis</topic><topic>Uterine Neoplasms - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KHAZAELI, M. B</creatorcontrib><creatorcontrib>BUCHINA, E. S</creatorcontrib><creatorcontrib>PATTILLO, R. A</creatorcontrib><creatorcontrib>SOONG, S.-J</creatorcontrib><creatorcontrib>HATCH, K. D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KHAZAELI, M. B</au><au>BUCHINA, E. S</au><au>PATTILLO, R. A</au><au>SOONG, S.-J</au><au>HATCH, K. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radioimmunoassay of free β-subunit of human chorionic gonadotropin in diagnosis of high-risk and low-risk gestational trophoblastic disease</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1989-02-01</date><risdate>1989</risdate><volume>160</volume><issue>2</issue><spage>444</spage><epage>449</epage><pages>444-449</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>A radioimmunoassay was performed with monoclonal antibody 1E5, which distinguishes free beta-subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotrophin. Serum samples were obtained from 68 pregnant women, 9 with hydatidiform mole who underwent spontaneous remission, 12 with hydatidiform mole who developed gestational trophoblastic disease, 5 with metastatic gestational trophoblastic disease of high-risk category, and 1 with choriocarcinoma concomitant with pregnancy. The concentrations of free beta-subunit of human chorionic gonadotropin and total beta-subunit were determined on the sera. The assay data were expressed as a ratio of nanograms of free beta-subunit per 1000 mIU of total beta-subunit. The ratios, analyzed by the Wilcoxon two-sample test, indicated a highly significant correlation between high ratios and the eventual diagnosis of high-risk gestational trophoblastic disease (p = 0.0019). This study suggests that the excessive production of free beta-subunit of human chorionic gonadotrophin may identify patients with high-risk gestational trophoblastic disease much earlier and identify gestational trophoblastic disease in patients during pregnancy.</abstract><cop>Philadelphia, PA</cop><pub>Elsevier</pub><pmid>2464935</pmid><doi>10.1016/0002-9378(89)90469-9</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0002-9378 |
| ispartof | American journal of obstetrics and gynecology, 1989-02, Vol.160 (2), p.444-449 |
| issn | 0002-9378 1097-6868 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Biological and medical sciences Chorionic Gonadotropin - blood Chorionic Gonadotropin, beta Subunit, Human Diseases of mother, fetus and pregnancy Female Gynecology. Andrology. Obstetrics Humans Hydatidiform Mole Medical sciences Middle Aged Neoplasms, Multiple Primary Peptide Fragments - blood Pregnancy Pregnancy. Fetus. Placenta Radioimmunoassay Risk Factors Trophoblastic Neoplasms - diagnosis Uterine Neoplasms - diagnosis |
| title | Radioimmunoassay of free β-subunit of human chorionic gonadotropin in diagnosis of high-risk and low-risk gestational trophoblastic disease |
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