Hemocyte population changes during the immune response of Aedes aegypti to inoculated microfilariae of Dirofilaria immitis
Ultrastructural and lectin-binding studies have established that the melanotic encapsulation reaction of Aedes aegypti Liverpool strain against inoculated Dirofilaria immitis microfilariae (mff) is a hemocyte-mediated reaction. Total hemocyte counts from mff-inoculated (=immune-activated), saline-in...
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| Veröffentlicht in: | The Journal of parasitology 1989-02, Vol.75 (1), p.119-123 |
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| creator | Christensen, B.M Huff, B.M Miranpuri, G.S Harris, K.L Christensen, L.A |
| description | Ultrastructural and lectin-binding studies have established that the melanotic encapsulation reaction of Aedes aegypti Liverpool strain against inoculated Dirofilaria immitis microfilariae (mff) is a hemocyte-mediated reaction. Total hemocyte counts from mff-inoculated (=immune-activated), saline-inoculated, and uninoculated female A. aegypti were determined using a hemocoel perfusion technique. Total hemocyte populations in uninoculated mosquitoes were significantly larger in younger mosquitoes, but no significant change was noted as mosquitoes aged beyond 14 days. Hemocyte populations in immune-activated mosquitoes increased from 1 to 3 days postinoculation (PI) and decreased on days 4 and 5 PI. Hemocyte populations at 1 to 4 days PI were significantly elevated in mff-inoculated A. aegypti as compared with saline-inoculated controls. Saline-inoculated mosquitoes displayed little change in total hemocyte numbers from 1 to 5 days PI, and their hemocyte populations were similar to those seen in uninoculated insects of the same age. Experiments involving the inoculation of [3H]thymidine along with mff or saline alone and studies involving the administration of colchicine suggest that increased hemocyte populations in immune-activated A. aegypti are a result of mitotic division of circulating blood cells. |
| doi_str_mv | 10.2307/3282948 |
| format | Article |
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Total hemocyte counts from mff-inoculated (=immune-activated), saline-inoculated, and uninoculated female A. aegypti were determined using a hemocoel perfusion technique. Total hemocyte populations in uninoculated mosquitoes were significantly larger in younger mosquitoes, but no significant change was noted as mosquitoes aged beyond 14 days. Hemocyte populations in immune-activated mosquitoes increased from 1 to 3 days postinoculation (PI) and decreased on days 4 and 5 PI. Hemocyte populations at 1 to 4 days PI were significantly elevated in mff-inoculated A. aegypti as compared with saline-inoculated controls. Saline-inoculated mosquitoes displayed little change in total hemocyte numbers from 1 to 5 days PI, and their hemocyte populations were similar to those seen in uninoculated insects of the same age. Experiments involving the inoculation of [3H]thymidine along with mff or saline alone and studies involving the administration of colchicine suggest that increased hemocyte populations in immune-activated A. aegypti are a result of mitotic division of circulating blood cells.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3282948</identifier><identifier>PMID: 2918431</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>AEDES ; Aedes - immunology ; Aedes - parasitology ; Aedes aegypti ; Animals ; Biological and medical sciences ; BLOOD CELLS ; Blood Cells - immunology ; Cell Division ; CELLULE SANGUINE ; CELULAS SANGUINEAS ; Colchicine - pharmacology ; Culicidae ; DIROFILARIA ; Dirofilaria immitis - immunology ; Diseases caused by nematodes ; Encapsulation ; Female ; Filarioidea - immunology ; Helminthic diseases ; Hemocytes ; Hemocytes - cytology ; Hemocytes - immunology ; Hemolymph ; IMMUNE RESPONSE ; Infectious diseases ; INOCULACION ; INOCULATION ; Larva migrans syndromes ; LARVAE ; LARVAS ; LARVE ; Medical sciences ; Microfilariae ; Mosquitos ; NEMATODE ; NEMATODES ; NEMATODOS ; Parasitic diseases ; Parasitology ; Pathogenesis and Host Response ; Perfusion ; Population dynamics ; Population growth ; REPONSE IMMUNITAIRE ; RESPUESTA INMUNOLOGICA ; Thymidine ; Tropical medicine</subject><ispartof>The Journal of parasitology, 1989-02, Vol.75 (1), p.119-123</ispartof><rights>Copyright 1989 American Society of Parasitologists</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-b6a063b9b22a0a0407b61d3b2c1ecb82d6a2989a735c0d8c6a065f8f27db36e33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3282948$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3282948$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7232108$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2918431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Christensen, B.M</creatorcontrib><creatorcontrib>Huff, B.M</creatorcontrib><creatorcontrib>Miranpuri, G.S</creatorcontrib><creatorcontrib>Harris, K.L</creatorcontrib><creatorcontrib>Christensen, L.A</creatorcontrib><title>Hemocyte population changes during the immune response of Aedes aegypti to inoculated microfilariae of Dirofilaria immitis</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Ultrastructural and lectin-binding studies have established that the melanotic encapsulation reaction of Aedes aegypti Liverpool strain against inoculated Dirofilaria immitis microfilariae (mff) is a hemocyte-mediated reaction. Total hemocyte counts from mff-inoculated (=immune-activated), saline-inoculated, and uninoculated female A. aegypti were determined using a hemocoel perfusion technique. Total hemocyte populations in uninoculated mosquitoes were significantly larger in younger mosquitoes, but no significant change was noted as mosquitoes aged beyond 14 days. Hemocyte populations in immune-activated mosquitoes increased from 1 to 3 days postinoculation (PI) and decreased on days 4 and 5 PI. Hemocyte populations at 1 to 4 days PI were significantly elevated in mff-inoculated A. aegypti as compared with saline-inoculated controls. Saline-inoculated mosquitoes displayed little change in total hemocyte numbers from 1 to 5 days PI, and their hemocyte populations were similar to those seen in uninoculated insects of the same age. Experiments involving the inoculation of [3H]thymidine along with mff or saline alone and studies involving the administration of colchicine suggest that increased hemocyte populations in immune-activated A. aegypti are a result of mitotic division of circulating blood cells.</description><subject>AEDES</subject><subject>Aedes - immunology</subject><subject>Aedes - parasitology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BLOOD CELLS</subject><subject>Blood Cells - immunology</subject><subject>Cell Division</subject><subject>CELLULE SANGUINE</subject><subject>CELULAS SANGUINEAS</subject><subject>Colchicine - pharmacology</subject><subject>Culicidae</subject><subject>DIROFILARIA</subject><subject>Dirofilaria immitis - immunology</subject><subject>Diseases caused by nematodes</subject><subject>Encapsulation</subject><subject>Female</subject><subject>Filarioidea - immunology</subject><subject>Helminthic diseases</subject><subject>Hemocytes</subject><subject>Hemocytes - cytology</subject><subject>Hemocytes - immunology</subject><subject>Hemolymph</subject><subject>IMMUNE RESPONSE</subject><subject>Infectious diseases</subject><subject>INOCULACION</subject><subject>INOCULATION</subject><subject>Larva migrans syndromes</subject><subject>LARVAE</subject><subject>LARVAS</subject><subject>LARVE</subject><subject>Medical sciences</subject><subject>Microfilariae</subject><subject>Mosquitos</subject><subject>NEMATODE</subject><subject>NEMATODES</subject><subject>NEMATODOS</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Pathogenesis and Host Response</subject><subject>Perfusion</subject><subject>Population dynamics</subject><subject>Population growth</subject><subject>REPONSE IMMUNITAIRE</subject><subject>RESPUESTA INMUNOLOGICA</subject><subject>Thymidine</subject><subject>Tropical medicine</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-L1TAQB_Agyvpcxbsg5CB6qiaTtkmOy_pjhQUPuucyTadvs7RNTdLD86-3z1eeNz2EEObDN8wMYy-leA9K6A8KDNjSPGI7aZUuQJXVY7YTAqBQylZP2bOUHoQQ1Xou2AVYaUold-zXDY3BHTLxOczLgNmHibt7nPaUeLdEP-15vifux3GZiEdKc5gS8dDzK-pWg7Q_zNnzHLifgjtGUMdH72Lo_YDR4x_80Z_fxyyffXrOnvQ4JHqx3Zfs7vOnH9c3xe23L1-vr24Lp0yZi7ZGUavWtgAoUJRCt7XsVAtOkmsNdDWCNRa1qpzojDvyqjc96K5VNSl1yd6ecucYfi6UcjP65GgYcKKwpEYbU2llxX-hrGCN1naF705wbTKlSH0zRz9iPDRSNMd1NNs6Vvl6i1zakbqz2-a_1t9sdUwOhz7i5Hw6Mw0KpDB_2UPKIf7jt1cn1mNocB_XpLvvxgoLda1-A46_pjQ</recordid><startdate>19890201</startdate><enddate>19890201</enddate><creator>Christensen, B.M</creator><creator>Huff, B.M</creator><creator>Miranpuri, G.S</creator><creator>Harris, K.L</creator><creator>Christensen, L.A</creator><general>American Society of Parasitologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19890201</creationdate><title>Hemocyte population changes during the immune response of Aedes aegypti to inoculated microfilariae of Dirofilaria immitis</title><author>Christensen, B.M ; Huff, B.M ; Miranpuri, G.S ; Harris, K.L ; Christensen, L.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-b6a063b9b22a0a0407b61d3b2c1ecb82d6a2989a735c0d8c6a065f8f27db36e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>AEDES</topic><topic>Aedes - immunology</topic><topic>Aedes - parasitology</topic><topic>Aedes aegypti</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BLOOD CELLS</topic><topic>Blood Cells - immunology</topic><topic>Cell Division</topic><topic>CELLULE SANGUINE</topic><topic>CELULAS SANGUINEAS</topic><topic>Colchicine - pharmacology</topic><topic>Culicidae</topic><topic>DIROFILARIA</topic><topic>Dirofilaria immitis - immunology</topic><topic>Diseases caused by nematodes</topic><topic>Encapsulation</topic><topic>Female</topic><topic>Filarioidea - immunology</topic><topic>Helminthic diseases</topic><topic>Hemocytes</topic><topic>Hemocytes - cytology</topic><topic>Hemocytes - immunology</topic><topic>Hemolymph</topic><topic>IMMUNE RESPONSE</topic><topic>Infectious diseases</topic><topic>INOCULACION</topic><topic>INOCULATION</topic><topic>Larva migrans syndromes</topic><topic>LARVAE</topic><topic>LARVAS</topic><topic>LARVE</topic><topic>Medical sciences</topic><topic>Microfilariae</topic><topic>Mosquitos</topic><topic>NEMATODE</topic><topic>NEMATODES</topic><topic>NEMATODOS</topic><topic>Parasitic diseases</topic><topic>Parasitology</topic><topic>Pathogenesis and Host Response</topic><topic>Perfusion</topic><topic>Population dynamics</topic><topic>Population growth</topic><topic>REPONSE IMMUNITAIRE</topic><topic>RESPUESTA INMUNOLOGICA</topic><topic>Thymidine</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Christensen, B.M</creatorcontrib><creatorcontrib>Huff, B.M</creatorcontrib><creatorcontrib>Miranpuri, G.S</creatorcontrib><creatorcontrib>Harris, K.L</creatorcontrib><creatorcontrib>Christensen, L.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Christensen, B.M</au><au>Huff, B.M</au><au>Miranpuri, G.S</au><au>Harris, K.L</au><au>Christensen, L.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hemocyte population changes during the immune response of Aedes aegypti to inoculated microfilariae of Dirofilaria immitis</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>1989-02-01</date><risdate>1989</risdate><volume>75</volume><issue>1</issue><spage>119</spage><epage>123</epage><pages>119-123</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>Ultrastructural and lectin-binding studies have established that the melanotic encapsulation reaction of Aedes aegypti Liverpool strain against inoculated Dirofilaria immitis microfilariae (mff) is a hemocyte-mediated reaction. Total hemocyte counts from mff-inoculated (=immune-activated), saline-inoculated, and uninoculated female A. aegypti were determined using a hemocoel perfusion technique. Total hemocyte populations in uninoculated mosquitoes were significantly larger in younger mosquitoes, but no significant change was noted as mosquitoes aged beyond 14 days. Hemocyte populations in immune-activated mosquitoes increased from 1 to 3 days postinoculation (PI) and decreased on days 4 and 5 PI. Hemocyte populations at 1 to 4 days PI were significantly elevated in mff-inoculated A. aegypti as compared with saline-inoculated controls. Saline-inoculated mosquitoes displayed little change in total hemocyte numbers from 1 to 5 days PI, and their hemocyte populations were similar to those seen in uninoculated insects of the same age. Experiments involving the inoculation of [3H]thymidine along with mff or saline alone and studies involving the administration of colchicine suggest that increased hemocyte populations in immune-activated A. aegypti are a result of mitotic division of circulating blood cells.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>2918431</pmid><doi>10.2307/3282948</doi><tpages>5</tpages></addata></record> |
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| ispartof | The Journal of parasitology, 1989-02, Vol.75 (1), p.119-123 |
| issn | 0022-3395 1937-2345 |
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| subjects | AEDES Aedes - immunology Aedes - parasitology Aedes aegypti Animals Biological and medical sciences BLOOD CELLS Blood Cells - immunology Cell Division CELLULE SANGUINE CELULAS SANGUINEAS Colchicine - pharmacology Culicidae DIROFILARIA Dirofilaria immitis - immunology Diseases caused by nematodes Encapsulation Female Filarioidea - immunology Helminthic diseases Hemocytes Hemocytes - cytology Hemocytes - immunology Hemolymph IMMUNE RESPONSE Infectious diseases INOCULACION INOCULATION Larva migrans syndromes LARVAE LARVAS LARVE Medical sciences Microfilariae Mosquitos NEMATODE NEMATODES NEMATODOS Parasitic diseases Parasitology Pathogenesis and Host Response Perfusion Population dynamics Population growth REPONSE IMMUNITAIRE RESPUESTA INMUNOLOGICA Thymidine Tropical medicine |
| title | Hemocyte population changes during the immune response of Aedes aegypti to inoculated microfilariae of Dirofilaria immitis |
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