Murine embryonal carcinoma cell-surface sialyl Lex is present on a novel glycoprotein and on high-molecular-weight lactosaminoglycan

Carbohydrate-specific monoclonal antibodies were used to demonstrate the expression of a new membrane glycoprotein on F9 murine embryonal carcinoma cells. Sialyl LeX was detected using monoclonal antibody FH6 in a sensitive, cell monolayer radioimmunoassay. The antigen codistributed in gel filtratio...

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Veröffentlicht in:	Experimental cell research 1989-02, Vol.180 (2), p.326-340
Hauptverfasser:	ROSENMAN, S. J, FENDERSON, B. A, HAKOMORI, S.-I
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Sugars - analysis Amino Sugars - immunology Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal Antigen-Antibody Reactions Antigens, Neoplasm - analysis Antigens, Neoplasm - immunology Antigens, Surface - analysis Antigens, Surface - immunology Biological and medical sciences Biomarkers, Tumor Cell Differentiation Cell Line Cell Membrane - analysis Chromatography, Affinity Embryonal Carcinoma Stem Cells Epitopes - analysis Epitopes - immunology Fundamental and applied biological sciences. Psychology Glycolipids - analysis Glycolipids - immunology Glycoproteins Lewis X Antigen Mice Molecular Weight Neoplastic Stem Cells - analysis Neoplastic Stem Cells - pathology Polysaccharides - analysis Polysaccharides - immunology Proteins Radioimmunoassay
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