Reactivation of the androgen receptor from murine preputial gland by thioredoxin or GSH
The androgen receptor from murine preputial gland was inactivated by density gradient centrifugation, affinity chromatography and isoelectric focusing. The hormone binding of the receptor could be partially restored (roughly 40%) by the thioredoxin-thioredoxinreductase system, by thioredoxin plus di...
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| Veröffentlicht in: | Journal of steroid biochemistry 1989, Vol.32 (1), p.53-58 |
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| container_end_page | 58 |
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| container_issue | 1 |
| container_start_page | 53 |
| container_title | Journal of steroid biochemistry |
| container_volume | 32 |
| creator | Wiegand, Manfred Ofenloch-Hähnle, Beatus Eisele, Karl |
| description | The androgen receptor from murine preputial gland was inactivated by density gradient centrifugation, affinity chromatography and isoelectric focusing. The hormone binding of the receptor could be partially restored (roughly 40%) by the thioredoxin-thioredoxinreductase system, by thioredoxin plus dithiothreitol or by glutathione. Dithiothreitol, by itself, was unable to reactivate the androgen receptor. These findings show that the receptor inactivation is, at least partially, due to thiol group oxidation and removal of SH-reducing and/or -stabilizing substances from the receptor during purification. |
| doi_str_mv | 10.1016/0022-4731(89)90013-7 |
| format | Article |
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Psychology ; Glutathione - pharmacology ; Isoelectric Focusing ; Kinetics ; Male ; Mice ; Molecular and cellular biology ; Penis - metabolism ; Receptors, Androgen - drug effects ; Receptors, Androgen - isolation & purification ; Receptors, Androgen - metabolism ; Sebaceous Glands - metabolism ; Thioredoxins - pharmacology</subject><ispartof>Journal of steroid biochemistry, 1989, Vol.32 (1), p.53-58</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-423f68d4771c62db1f6d4feb5a0499e93c9d9d90d76b4961e09f374a4afc6fbc3</citedby><cites>FETCH-LOGICAL-c417t-423f68d4771c62db1f6d4feb5a0499e93c9d9d90d76b4961e09f374a4afc6fbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6856135$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2913401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wiegand, Manfred</creatorcontrib><creatorcontrib>Ofenloch-Hähnle, Beatus</creatorcontrib><creatorcontrib>Eisele, Karl</creatorcontrib><title>Reactivation of the androgen receptor from murine preputial gland by thioredoxin or GSH</title><title>Journal of steroid biochemistry</title><addtitle>J Steroid Biochem</addtitle><description>The androgen receptor from murine preputial gland was inactivated by density gradient centrifugation, affinity chromatography and isoelectric focusing. The hormone binding of the receptor could be partially restored (roughly 40%) by the thioredoxin-thioredoxinreductase system, by thioredoxin plus dithiothreitol or by glutathione. Dithiothreitol, by itself, was unable to reactivate the androgen receptor. These findings show that the receptor inactivation is, at least partially, due to thiol group oxidation and removal of SH-reducing and/or -stabilizing substances from the receptor during purification.</description><subject>Animals</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromatography, Affinity</subject><subject>Cytosol - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione - pharmacology</subject><subject>Isoelectric Focusing</subject><subject>Kinetics</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Penis - metabolism</subject><subject>Receptors, Androgen - drug effects</subject><subject>Receptors, Androgen - isolation & purification</subject><subject>Receptors, Androgen - metabolism</subject><subject>Sebaceous Glands - metabolism</subject><subject>Thioredoxins - pharmacology</subject><issn>0022-4731</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFLHDEUx3NosVv1G1TIoRQ9jCYzmWRyKYjoriAIttJjyCQva8rMZJrMiH57s-6yx0oOgfd-_8fj9xD6Rsk5JZRfEFKWBRMVPW3kmSSEVoX4hBb78hf0NaW_uS4bVh6gg1LSihG6QH8eQJvJP-vJhwEHh6cnwHqwMaxhwBEMjFOI2MXQ436OfgA8RhjnyesOr7tM4vY1h3yIYMOLzzMiXv5aHaHPTncJjnf_IXq8uf59tSru7pe3V5d3hWFUTAUrK8cby4Sghpe2pY5b5qCtNWFSgqyMtPkRK3jLJKdApKsE00w7w11rqkP0Yzt3jOHfDGlSvU8GurwZhDkp0TSsZrX8EKQ1JUJykkG2BU0MKUVwaoy-1_FVUaI2stXGqtpYVY1U77KVyLGT3fy57cHuQzvTuf9919fJ6M5FPRif9hhvak6rOmM_txhkac8eokrGw2DA-nyMSdng_7_HG1-XnQw</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Wiegand, Manfred</creator><creator>Ofenloch-Hähnle, Beatus</creator><creator>Eisele, Karl</creator><general>Elsevier B.V</general><general>Pergamon</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Reactivation of the androgen receptor from murine preputial gland by thioredoxin or GSH</title><author>Wiegand, Manfred ; Ofenloch-Hähnle, Beatus ; Eisele, Karl</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-423f68d4771c62db1f6d4feb5a0499e93c9d9d90d76b4961e09f374a4afc6fbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromatography, Affinity</topic><topic>Cytosol - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione - pharmacology</topic><topic>Isoelectric Focusing</topic><topic>Kinetics</topic><topic>Male</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Penis - metabolism</topic><topic>Receptors, Androgen - drug effects</topic><topic>Receptors, Androgen - isolation & purification</topic><topic>Receptors, Androgen - metabolism</topic><topic>Sebaceous Glands - metabolism</topic><topic>Thioredoxins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wiegand, Manfred</creatorcontrib><creatorcontrib>Ofenloch-Hähnle, Beatus</creatorcontrib><creatorcontrib>Eisele, Karl</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of steroid biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wiegand, Manfred</au><au>Ofenloch-Hähnle, Beatus</au><au>Eisele, Karl</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reactivation of the androgen receptor from murine preputial gland by thioredoxin or GSH</atitle><jtitle>Journal of steroid biochemistry</jtitle><addtitle>J Steroid Biochem</addtitle><date>1989</date><risdate>1989</risdate><volume>32</volume><issue>1</issue><spage>53</spage><epage>58</epage><pages>53-58</pages><issn>0022-4731</issn><coden>JSTBBK</coden><abstract>The androgen receptor from murine preputial gland was inactivated by density gradient centrifugation, affinity chromatography and isoelectric focusing. The hormone binding of the receptor could be partially restored (roughly 40%) by the thioredoxin-thioredoxinreductase system, by thioredoxin plus dithiothreitol or by glutathione. Dithiothreitol, by itself, was unable to reactivate the androgen receptor. These findings show that the receptor inactivation is, at least partially, due to thiol group oxidation and removal of SH-reducing and/or -stabilizing substances from the receptor during purification.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2913401</pmid><doi>10.1016/0022-4731(89)90013-7</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0022-4731 |
| ispartof | Journal of steroid biochemistry, 1989, Vol.32 (1), p.53-58 |
| issn | 0022-4731 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78845459 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Bacterial Proteins - pharmacology Biological and medical sciences Cell receptors Cell structures and functions Centrifugation, Density Gradient Chromatography, Affinity Cytosol - metabolism Fundamental and applied biological sciences. Psychology Glutathione - pharmacology Isoelectric Focusing Kinetics Male Mice Molecular and cellular biology Penis - metabolism Receptors, Androgen - drug effects Receptors, Androgen - isolation & purification Receptors, Androgen - metabolism Sebaceous Glands - metabolism Thioredoxins - pharmacology |
| title | Reactivation of the androgen receptor from murine preputial gland by thioredoxin or GSH |
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