Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients

Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequen...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1989-01, Vol.243 (4890), p.529-533
Hauptverfasser:	Reddy, E. Premkumar, Sandberg-Wollheim, Magnhild, Mettus, Richard V., Ray, Phillip E., DeFreitas, Elaine, Koprowski, Hilary
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult AIDS/HIV Analysis Antibodies Base Sequence Biological and medical sciences Blood cells Cell lines Child Cloning, Molecular DNA DNA Restriction Enzymes DNA, Viral - genetics Female Gene Amplification Genetic aspects Genetics Genomes HIV HIV (Viruses) Human T lymphotropic virus 1 Human T-lymphotropic virus 1 - genetics Humans Leukocytes Leukocytes, Mononuclear - analysis Leukocytes, Mononuclear - microbiology Lymphocytes Macrophages - analysis Macrophages - microbiology Male Medical research Medical sciences Molecular Sequence Data Monocytes Multiple sclerosis Multiple Sclerosis - microbiology Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Neurology Nucleic Acid Hybridization Oligonucleotide Probes Plasmids T lymphocytes Tests Viruses
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