Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients
Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequen...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1989-01, Vol.243 (4890), p.529-533 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Reddy, E. Premkumar Sandberg-Wollheim, Magnhild Mettus, Richard V. Ray, Phillip E. DeFreitas, Elaine Koprowski, Hilary |
| description | Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome. |
| doi_str_mv | 10.1126/science.2536193 |
| format | Article |
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Premkumar ; Sandberg-Wollheim, Magnhild ; Mettus, Richard V. ; Ray, Phillip E. ; DeFreitas, Elaine ; Koprowski, Hilary</creator><creatorcontrib>Reddy, E. Premkumar ; Sandberg-Wollheim, Magnhild ; Mettus, Richard V. ; Ray, Phillip E. ; DeFreitas, Elaine ; Koprowski, Hilary</creatorcontrib><description>Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.2536193</identifier><identifier>PMID: 2536193</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>Adolescent ; Adult ; AIDS/HIV ; Analysis ; Antibodies ; Base Sequence ; Biological and medical sciences ; Blood cells ; Cell lines ; Child ; Cloning, Molecular ; DNA ; DNA Restriction Enzymes ; DNA, Viral - genetics ; Female ; Gene Amplification ; Genetic aspects ; Genetics ; Genomes ; HIV ; HIV (Viruses) ; Human T lymphotropic virus 1 ; Human T-lymphotropic virus 1 - genetics ; Humans ; Leukocytes ; Leukocytes, Mononuclear - analysis ; Leukocytes, Mononuclear - microbiology ; Lymphocytes ; Macrophages - analysis ; Macrophages - microbiology ; Male ; Medical research ; Medical sciences ; Molecular Sequence Data ; Monocytes ; Multiple sclerosis ; Multiple Sclerosis - microbiology ; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis ; Neurology ; Nucleic Acid Hybridization ; Oligonucleotide Probes ; Plasmids ; T lymphocytes ; Tests ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1989-01, Vol.243 (4890), p.529-533</ispartof><rights>Copyright 1989 The American Association for the Advancement of Science</rights><rights>1989 INIST-CNRS</rights><rights>COPYRIGHT 1989 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1989 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Jan 27, 1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c729t-8ce44604c76a5123528ef2c2b1429d45507d7cd9948c8fa26575b78fa4afdbdc3</citedby><cites>FETCH-LOGICAL-c729t-8ce44604c76a5123528ef2c2b1429d45507d7cd9948c8fa26575b78fa4afdbdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1703222$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1703222$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7307421$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2536193$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reddy, E. Premkumar</creatorcontrib><creatorcontrib>Sandberg-Wollheim, Magnhild</creatorcontrib><creatorcontrib>Mettus, Richard V.</creatorcontrib><creatorcontrib>Ray, Phillip E.</creatorcontrib><creatorcontrib>DeFreitas, Elaine</creatorcontrib><creatorcontrib>Koprowski, Hilary</creatorcontrib><title>Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.</description><subject>Adolescent</subject><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Analysis</subject><subject>Antibodies</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blood cells</subject><subject>Cell lines</subject><subject>Child</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Viral - genetics</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Genetic aspects</subject><subject>Genetics</subject><subject>Genomes</subject><subject>HIV</subject><subject>HIV (Viruses)</subject><subject>Human T lymphotropic virus 1</subject><subject>Human T-lymphotropic virus 1 - genetics</subject><subject>Humans</subject><subject>Leukocytes</subject><subject>Leukocytes, Mononuclear - analysis</subject><subject>Leukocytes, Mononuclear - microbiology</subject><subject>Lymphocytes</subject><subject>Macrophages - analysis</subject><subject>Macrophages - microbiology</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Monocytes</subject><subject>Multiple sclerosis</subject><subject>Multiple Sclerosis - microbiology</subject><subject>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. 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Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</topic><topic>Neurology</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Probes</topic><topic>Plasmids</topic><topic>T lymphocytes</topic><topic>Tests</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reddy, E. 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Premkumar</au><au>Sandberg-Wollheim, Magnhild</au><au>Mettus, Richard V.</au><au>Ray, Phillip E.</au><au>DeFreitas, Elaine</au><au>Koprowski, Hilary</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1989-01-27</date><risdate>1989</risdate><volume>243</volume><issue>4890</issue><spage>529</spage><epage>533</epage><pages>529-533</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Techniques of gene amplification, molecular cloning, and sequence analysis were used to test for the presence of sequences related to human T-lymphotropic virus type I (HTLV-I) in peripheral blood mononuclear cells of six patients with multiple sclerosis (MS) and 20 normal individuals. HTLV-I sequences were detected in all six MS patients and in one individual from the control group by DNA blot analysis and molecular cloning of amplified DNAs. The viral sequences in MS patients were associated with adherent cell populations consisting predominantly of monocytes and macrophages. Molecular cloning and nucleotide sequence analysis indicated that these amplified viral sequences were related to the HTLV-I proviral genome.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>2536193</pmid><doi>10.1126/science.2536193</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1989-01, Vol.243 (4890), p.529-533 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78845302 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Adolescent Adult AIDS/HIV Analysis Antibodies Base Sequence Biological and medical sciences Blood cells Cell lines Child Cloning, Molecular DNA DNA Restriction Enzymes DNA, Viral - genetics Female Gene Amplification Genetic aspects Genetics Genomes HIV HIV (Viruses) Human T lymphotropic virus 1 Human T-lymphotropic virus 1 - genetics Humans Leukocytes Leukocytes, Mononuclear - analysis Leukocytes, Mononuclear - microbiology Lymphocytes Macrophages - analysis Macrophages - microbiology Male Medical research Medical sciences Molecular Sequence Data Monocytes Multiple sclerosis Multiple Sclerosis - microbiology Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Neurology Nucleic Acid Hybridization Oligonucleotide Probes Plasmids T lymphocytes Tests Viruses |
| title | Amplification and Molecular Cloning of HTLV-I Sequences from DNA of Multiple Sclerosis Patients |
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