Direct Measurement of Single-Molecule Diffusion and Photodecomposition in Free Solution

Continuous monitoring of submillisecond free-solution dynamics of individual rhodamine-6G molecules and 30-base single-stranded DNA tagged with rhodamine was achieved. Fluorescence images were recorded from the same set of isolated molecules excited either through the evanescent field at the quartz-...

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Veröffentlicht in:	Science 1997-02, Vol.275 (5303), p.1106-1109
Hauptverfasser:	Xu, Xiao-Hong, Yeung, Edward S.
Format:	Artikel
Sprache:	eng
Schlagworte:	40 CHEMISTRY Chemistry DIFFUSION Diffusion (Physics) Diffusion coefficient Direct measurement DNA DNA, Single-Stranded - analysis DNA, Single-Stranded - chemistry Electrons Exact sciences and technology Fluorescence Fluorescent Dyes - analysis Fluorescent Dyes - chemistry General and physical chemistry Laboratory Equipment Laser power Lasers Measurement Microscopes Microscopy, Video Molecular Biology Molecular dynamics Molecules Observation PHOTOCHEMICAL REACTIONS Photochemistry Photodecomposition Photolysis Physical chemistry of induced reactions (with radiations, particles and ultrasonics) Pixels Research Design Rhodamines - analysis Rhodamines - chemistry Solution properties Solutions Water
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