Biosynthesis and Incorporation into Protein of Norleucine by Escherichia coli

The methionine analog norleucine was produced during the synthesis of bovine somatotropin by Escherichia coli strain W3110G containing the recombinant plasmid pBGH1. Norleucine was generated by the leucine biosynthetic pathway from pyruvate or α-ketobutyrate in place of α-ketoisovalerate as the init...

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Veröffentlicht in:The Journal of biological chemistry 1989-01, Vol.264 (1), p.531-539
Hauptverfasser: Bogosian, G, Violand, B N, Dorward-King, E J, Workman, W E, Jung, P E, Kane, J F
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container_end_page 539
container_issue 1
container_start_page 531
container_title The Journal of biological chemistry
container_volume 264
creator Bogosian, G
Violand, B N
Dorward-King, E J
Workman, W E
Jung, P E
Kane, J F
description The methionine analog norleucine was produced during the synthesis of bovine somatotropin by Escherichia coli strain W3110G containing the recombinant plasmid pBGH1. Norleucine was generated by the leucine biosynthetic pathway from pyruvate or α-ketobutyrate in place of α-ketoisovalerate as the initial substrate. The intracellular level of norleucine was high enough to permit the analog to compete successfully with methionine for incorporation into protein. Two ways were found to prevent either the formation of norleucine or its incorporation into protein. The endogenous synthesis of norleucine was eliminated by deleting the leucine Operon. The addition of sufficient methionine or 2-hydroxy-4-methylthiobutanoic acid, a precursor of methionine, to the culture medium prevented any norleucine from being incorporated into protein.
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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Amino Acids - analysis
Aminoacids, peptides. Hormones. Neuropeptides
Aminocaproates - biosynthesis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cattle
Chromatography, High Pressure Liquid
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Fermentation
Fundamental and applied biological sciences. Psychology
Genotype
Growth Hormone - biosynthesis
Norleucine - biosynthesis
Norleucine - metabolism
Plasmids
Proteins
Recombinant Proteins - biosynthesis
Transduction, Genetic
title Biosynthesis and Incorporation into Protein of Norleucine by Escherichia coli
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