Effective detection of active HCV infection: HCV RNA carrier state in a context free of hepatitis symptoms
HCV immunological assays have limited specificity due to considerable variability of genomic coding sequences. Accordingly, PCR RNA detection also shows variable incidence of HCV in a non-A, non-B (NANB) hepatitis context. We used in-house designed nested PCR applying primers from the 5′ untranslate...
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| Veröffentlicht in: | Clinica chimica acta 1997-02, Vol.258 (1), p.91-104 |
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| container_title | Clinica chimica acta |
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| creator | Arapinis, Calliope Kostaridou, Stavroula Kattamis, Christos |
| description | HCV immunological assays have limited specificity due to considerable variability of genomic coding sequences. Accordingly, PCR RNA detection also shows variable incidence of HCV in a non-A, non-B (NANB) hepatitis context. We used in-house designed nested PCR applying primers from the 5′ untranslated region in 150 thalassemic patients classified in four groups according to anti-HCV screening and glutamic-pyruvate transaminase (GPT) levels. Group A: anti-HCV
+/high GPT levels; group B: anti-HCV
+/normal GPT levels; group C: anti-HCV
−/high GPT levels; group D: anti-HCV
−/normal GPT levels. Viral incidence and concentration, both high in group A, decreased towards group D. Group C RNA incidence was unexpectedly high and, moreover, one control case proved HCV-RNA
+. Compared with the Amplicor kit our primers were considerably more sensitive. |
| doi_str_mv | 10.1016/S0009-8981(96)06448-0 |
| format | Article |
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+/high GPT levels; group B: anti-HCV
+/normal GPT levels; group C: anti-HCV
−/high GPT levels; group D: anti-HCV
−/normal GPT levels. Viral incidence and concentration, both high in group A, decreased towards group D. Group C RNA incidence was unexpectedly high and, moreover, one control case proved HCV-RNA
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+/high GPT levels; group B: anti-HCV
+/normal GPT levels; group C: anti-HCV
−/high GPT levels; group D: anti-HCV
−/normal GPT levels. Viral incidence and concentration, both high in group A, decreased towards group D. Group C RNA incidence was unexpectedly high and, moreover, one control case proved HCV-RNA
+. Compared with the Amplicor kit our primers were considerably more sensitive.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Asymptomatic, anti-HCV − HCV carrier</subject><subject>Carrier State - diagnosis</subject><subject>Carrier State - virology</subject><subject>Child</subject><subject>Female</subject><subject>Hepacivirus - genetics</subject><subject>Hepatitis C - diagnosis</subject><subject>Hepatitis C - etiology</subject><subject>Hepatitis C - virology</subject><subject>Hepatitis C virus (HCV)</subject><subject>Humans</subject><subject>Male</subject><subject>Non-A, non-B hepatitis (NANB)</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Viral - analysis</subject><subject>Thalassemia - complications</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQx4MoWh8fQchJ9LCa3U2ziRcpxRcUBV_XkCYTTOnu1iQt9tub7ZZevczzPzPMD6HznFznJGc374QQkXHB80vBrgijlGdkDw1yXpVZSUWxjwY7yRE6DmGWUkpYfogOBaGCUjZAs3trQUe3AmwgdlHb4NZi1deexl_YNbav327St5cR1sp7Bx6HqCIkAVZYt02E34itB-gWfMNCRRddwGFdL2Jbh1N0YNU8wNnWn6DPh_uP8VM2eX18Ho8mmS4JjxnooiAFA8YNZ0wXojLKCgHTIbfGUCW0oWJYlVyVVJGhgVynl5PRXExVRcsTdNHvXfj2ZwkhytoFDfO5aqBdBllxTglnnXDYC7VvQ_Bg5cK7Wvm1zInsGMsNY9kBlILJDWNJ0tz59sByWoPZTW2hpv5d34f05SpxkkE7aDQY5xNJaVr3z4U_rkWL8Q</recordid><startdate>19970203</startdate><enddate>19970203</enddate><creator>Arapinis, Calliope</creator><creator>Kostaridou, Stavroula</creator><creator>Kattamis, Christos</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970203</creationdate><title>Effective detection of active HCV infection: HCV RNA carrier state in a context free of hepatitis symptoms</title><author>Arapinis, Calliope ; Kostaridou, Stavroula ; Kattamis, Christos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-ec22026e68d866c297daf99eb58fdd4a9cd495738a34a05de1c1871c1c89ba743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Asymptomatic, anti-HCV − HCV carrier</topic><topic>Carrier State - diagnosis</topic><topic>Carrier State - virology</topic><topic>Child</topic><topic>Female</topic><topic>Hepacivirus - genetics</topic><topic>Hepatitis C - diagnosis</topic><topic>Hepatitis C - etiology</topic><topic>Hepatitis C - virology</topic><topic>Hepatitis C virus (HCV)</topic><topic>Humans</topic><topic>Male</topic><topic>Non-A, non-B hepatitis (NANB)</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Viral - analysis</topic><topic>Thalassemia - complications</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arapinis, Calliope</creatorcontrib><creatorcontrib>Kostaridou, Stavroula</creatorcontrib><creatorcontrib>Kattamis, Christos</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arapinis, Calliope</au><au>Kostaridou, Stavroula</au><au>Kattamis, Christos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effective detection of active HCV infection: HCV RNA carrier state in a context free of hepatitis symptoms</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1997-02-03</date><risdate>1997</risdate><volume>258</volume><issue>1</issue><spage>91</spage><epage>104</epage><pages>91-104</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>HCV immunological assays have limited specificity due to considerable variability of genomic coding sequences. Accordingly, PCR RNA detection also shows variable incidence of HCV in a non-A, non-B (NANB) hepatitis context. We used in-house designed nested PCR applying primers from the 5′ untranslated region in 150 thalassemic patients classified in four groups according to anti-HCV screening and glutamic-pyruvate transaminase (GPT) levels. Group A: anti-HCV
+/high GPT levels; group B: anti-HCV
+/normal GPT levels; group C: anti-HCV
−/high GPT levels; group D: anti-HCV
−/normal GPT levels. Viral incidence and concentration, both high in group A, decreased towards group D. Group C RNA incidence was unexpectedly high and, moreover, one control case proved HCV-RNA
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| ispartof | Clinica chimica acta, 1997-02, Vol.258 (1), p.91-104 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78840864 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adolescent Adult Asymptomatic, anti-HCV − HCV carrier Carrier State - diagnosis Carrier State - virology Child Female Hepacivirus - genetics Hepatitis C - diagnosis Hepatitis C - etiology Hepatitis C - virology Hepatitis C virus (HCV) Humans Male Non-A, non-B hepatitis (NANB) Polymerase Chain Reaction RNA, Viral - analysis Thalassemia - complications |
| title | Effective detection of active HCV infection: HCV RNA carrier state in a context free of hepatitis symptoms |
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