Production of monoclonal antibody to human IgG allotype G3M T

An efficient method for the production of monoclonal anti-G3M T antibody is described. IgG3 protein of GM B3ST phenotype was isolated by affinity chromatography on Ricinus communis lectin I-agarose and used for immunization. A mouse hybridoma clone was obtained by fusion of popliteal lymph node cell...

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Veröffentlicht in:	Journal of immunological methods 1989-01, Vol.116 (1), p.99-103
Hauptverfasser:	Kimura, Akihiko, Tamaki, Yoshihiro, Kishida, Tetsuko, Fukuda, Masako, Tsuji, Tsutomu
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-G3M T Antibodies, immunoglobulins Antibodies, Monoclonal - immunology Antibody Specificity Biological and medical sciences Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Genetic markers (allotypes, idiotypes) GM allotyping Hemagglutination Inhibition Tests Humans Immunoglobulin Allotypes - immunology Immunoglobulin G - immunology Molecular immunology Monoclonal antibody
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