Production of monoclonal antibody to human IgG allotype G3M T
An efficient method for the production of monoclonal anti-G3M T antibody is described. IgG3 protein of GM B3ST phenotype was isolated by affinity chromatography on Ricinus communis lectin I-agarose and used for immunization. A mouse hybridoma clone was obtained by fusion of popliteal lymph node cell...
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| Veröffentlicht in: | Journal of immunological methods 1989-01, Vol.116 (1), p.99-103 |
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| container_end_page | 103 |
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| container_title | Journal of immunological methods |
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| creator | Kimura, Akihiko Tamaki, Yoshihiro Kishida, Tetsuko Fukuda, Masako Tsuji, Tsutomu |
| description | An efficient method for the production of monoclonal anti-G3M T antibody is described. IgG3 protein of GM B3ST phenotype was isolated by affinity chromatography on
Ricinus communis lectin I-agarose and used for immunization. A mouse hybridoma clone was obtained by fusion of popliteal lymph node cells and P3U1 myeloma cells. The antibody produced was tested for allotype specificity by hemagglutination inhibition and ELISA methods using 101 IgG-allotyping control sera. The antibody was neutralizable by all G3M T-positive sera and entirely nonneutralizable by G3M T-negative sera in the inhibition test, and reacted only with G3M T-positive IgG coats in the ELISA test. The results prove the antibody to be allotype-specific, and therefore practically establishes its monoclonality. |
| doi_str_mv | 10.1016/0022-1759(89)90317-7 |
| format | Article |
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Ricinus communis lectin I-agarose and used for immunization. A mouse hybridoma clone was obtained by fusion of popliteal lymph node cells and P3U1 myeloma cells. The antibody produced was tested for allotype specificity by hemagglutination inhibition and ELISA methods using 101 IgG-allotyping control sera. The antibody was neutralizable by all G3M T-positive sera and entirely nonneutralizable by G3M T-negative sera in the inhibition test, and reacted only with G3M T-positive IgG coats in the ELISA test. The results prove the antibody to be allotype-specific, and therefore practically establishes its monoclonality.</description><subject>Anti-G3M T</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Genetic markers (allotypes, idiotypes)</subject><subject>GM allotyping</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Humans</subject><subject>Immunoglobulin Allotypes - immunology</subject><subject>Immunoglobulin G - immunology</subject><subject>Molecular immunology</subject><subject>Monoclonal antibody</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rGzEQhkVpcR0n_6AFHUpIDttopF1LOrQQTOIaHJKDcxZafbQquytX2i3432cdGx8T3sMc3meG4UHoC5DvQGB-QwilBfBKXgl5LQkDXvAPaAqC04JLUn1E0xPyGZ3l_JcQAmROJmhCJVRA-RT9eErRDqYPscPR4zZ20TSx0w3WXR_qaHe4j_jP0OoOr34vsW6a2O-2Di_ZA96co09eN9ldHOcMPd_fbRa_ivXjcrW4XRemBN4X1NcEtKeOW8lKVkk_50xa5sXc19ZqXUPJJTDKKAUG3pVe0zHCC2s4M2yGLg93tyn-G1zuVRuycU2jOxeHrLgQbIx4F4SKlrTk1QiWB9CkmHNyXm1TaHXaKSBqr1ft3am9OyWketWr-Lj29Xh_qFtnT0tHn2P_7djrbHTjk-5MyCeMs4oChxH7ecDcKO1_cEllE1xnnA3JmV7ZGN7-4wV1jJRc</recordid><startdate>19890106</startdate><enddate>19890106</enddate><creator>Kimura, Akihiko</creator><creator>Tamaki, Yoshihiro</creator><creator>Kishida, Tetsuko</creator><creator>Fukuda, Masako</creator><creator>Tsuji, Tsutomu</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19890106</creationdate><title>Production of monoclonal antibody to human IgG allotype G3M T</title><author>Kimura, Akihiko ; Tamaki, Yoshihiro ; Kishida, Tetsuko ; Fukuda, Masako ; Tsuji, Tsutomu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-2fb01af2e7d934359f6739d3f86fbddaab1479132322131fe4fa2a2a8f8dc73c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Anti-G3M T</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Genetic markers (allotypes, idiotypes)</topic><topic>GM allotyping</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Humans</topic><topic>Immunoglobulin Allotypes - immunology</topic><topic>Immunoglobulin G - immunology</topic><topic>Molecular immunology</topic><topic>Monoclonal antibody</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Akihiko</creatorcontrib><creatorcontrib>Tamaki, Yoshihiro</creatorcontrib><creatorcontrib>Kishida, Tetsuko</creatorcontrib><creatorcontrib>Fukuda, Masako</creatorcontrib><creatorcontrib>Tsuji, Tsutomu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Akihiko</au><au>Tamaki, Yoshihiro</au><au>Kishida, Tetsuko</au><au>Fukuda, Masako</au><au>Tsuji, Tsutomu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of monoclonal antibody to human IgG allotype G3M T</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1989-01-06</date><risdate>1989</risdate><volume>116</volume><issue>1</issue><spage>99</spage><epage>103</epage><pages>99-103</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>An efficient method for the production of monoclonal anti-G3M T antibody is described. IgG3 protein of GM B3ST phenotype was isolated by affinity chromatography on
Ricinus communis lectin I-agarose and used for immunization. A mouse hybridoma clone was obtained by fusion of popliteal lymph node cells and P3U1 myeloma cells. The antibody produced was tested for allotype specificity by hemagglutination inhibition and ELISA methods using 101 IgG-allotyping control sera. The antibody was neutralizable by all G3M T-positive sera and entirely nonneutralizable by G3M T-negative sera in the inhibition test, and reacted only with G3M T-positive IgG coats in the ELISA test. The results prove the antibody to be allotype-specific, and therefore practically establishes its monoclonality.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2915127</pmid><doi>10.1016/0022-1759(89)90317-7</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1989-01, Vol.116 (1), p.99-103 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-G3M T Antibodies, immunoglobulins Antibodies, Monoclonal - immunology Antibody Specificity Biological and medical sciences Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Genetic markers (allotypes, idiotypes) GM allotyping Hemagglutination Inhibition Tests Humans Immunoglobulin Allotypes - immunology Immunoglobulin G - immunology Molecular immunology Monoclonal antibody |
| title | Production of monoclonal antibody to human IgG allotype G3M T |
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