The elimination of malignant B lymphocytes from human bone marrow using monoclonal antibodies DLC-48 and LN-1 and human serum: A preclinical study
Monoclonal antibodies DLC-48 and LN-1 were evaluated for use in purging malignant lymphoma cells from human bone marrow. Using a 51Cr-release assay and sensitive clonogenic assay for the SU-DHL-2 and -4 cell lines, it was established that greater than four logs of malignant lymphoid cells can be eli...
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| Veröffentlicht in: | Leukemia research 1989, Vol.13 (1), p.71-76 |
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| container_title | Leukemia research |
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| creator | Winter, Jane N. Epstein, Alan L. Bass, Bonny Marder, Robert J. |
| description | Monoclonal antibodies DLC-48 and LN-1 were evaluated for use in purging malignant lymphoma cells from human bone marrow. Using a 51Cr-release assay and sensitive clonogenic assay for the SU-DHL-2 and -4 cell lines, it was established that greater than four logs of malignant lymphoid cells can be eliminated from human bone marrow autografts with three treatments of antibody and autologous human serum at a final cell concentration of 1 × 107cells/ml. A combination of DLC-48 and LN-1 was more effective than either antibody alone. Treatment with antibody and autologous serum had no effect on the growth of human hematopoietic progenitor cells. The clinical effects of marrow treatment with DLC-48 and LN-1 will be evaluated in upcoming clinical trials. |
| doi_str_mv | 10.1016/0145-2126(89)90033-7 |
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Using a 51Cr-release assay and sensitive clonogenic assay for the SU-DHL-2 and -4 cell lines, it was established that greater than four logs of malignant lymphoid cells can be eliminated from human bone marrow autografts with three treatments of antibody and autologous human serum at a final cell concentration of 1 × 107cells/ml. A combination of DLC-48 and LN-1 was more effective than either antibody alone. Treatment with antibody and autologous serum had no effect on the growth of human hematopoietic progenitor cells. The clinical effects of marrow treatment with DLC-48 and LN-1 will be evaluated in upcoming clinical trials.</description><identifier>ISSN: 0145-2126</identifier><identifier>EISSN: 1873-5835</identifier><identifier>DOI: 10.1016/0145-2126(89)90033-7</identifier><identifier>PMID: 2644495</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Monoclonal ; autologous bone marrow transplantation ; B-Lymphocytes - cytology ; Blood ; Bone Marrow Cells ; Bone Marrow Transplantation ; Cell Separation - methods ; Humans ; Lymphoma, Non-Hodgkin - pathology ; marrow purging ; Mice ; Monoclonal antibodies ; non-Hodgkin's lymphoma</subject><ispartof>Leukemia research, 1989, Vol.13 (1), p.71-76</ispartof><rights>1989 Pergamon Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c221t-2db9935683ac000dfb3a1eb3a746626e19faffda83988846080d9d3ac382234d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0145-2126(89)90033-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2644495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Winter, Jane N.</creatorcontrib><creatorcontrib>Epstein, Alan L.</creatorcontrib><creatorcontrib>Bass, Bonny</creatorcontrib><creatorcontrib>Marder, Robert J.</creatorcontrib><title>The elimination of malignant B lymphocytes from human bone marrow using monoclonal antibodies DLC-48 and LN-1 and human serum: A preclinical study</title><title>Leukemia research</title><addtitle>Leuk Res</addtitle><description>Monoclonal antibodies DLC-48 and LN-1 were evaluated for use in purging malignant lymphoma cells from human bone marrow. Using a 51Cr-release assay and sensitive clonogenic assay for the SU-DHL-2 and -4 cell lines, it was established that greater than four logs of malignant lymphoid cells can be eliminated from human bone marrow autografts with three treatments of antibody and autologous human serum at a final cell concentration of 1 × 107cells/ml. A combination of DLC-48 and LN-1 was more effective than either antibody alone. Treatment with antibody and autologous serum had no effect on the growth of human hematopoietic progenitor cells. The clinical effects of marrow treatment with DLC-48 and LN-1 will be evaluated in upcoming clinical trials.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>autologous bone marrow transplantation</subject><subject>B-Lymphocytes - cytology</subject><subject>Blood</subject><subject>Bone Marrow Cells</subject><subject>Bone Marrow Transplantation</subject><subject>Cell Separation - methods</subject><subject>Humans</subject><subject>Lymphoma, Non-Hodgkin - pathology</subject><subject>marrow purging</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>non-Hodgkin's lymphoma</subject><issn>0145-2126</issn><issn>1873-5835</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UctuFDEQtBAoLIE_AMknBIcBv2bG5hApLBCQVnAJZ8tj92SNPPZizyTa3-CL8WZXOebSbrmrqlXVCL2m5AMltPtIqGgbRln3Tqr3ihDOm_4JWlHZ86aVvH2KVg-Q5-hFKX8IIa2i6gydsU4IodoV-ne9BQzBTz6a2aeI04gnE_xNNHHGn3HYT7ttsvsZCh5zmvB2mUzEQ4pQcTmnO7wUH2_wlGKyIUUTcGX6ITlfKV8260bI-uPw5mdD75ujQoG8TJ_wJd5lsMFHbyuzzIvbv0TPRhMKvDq95-j3t6_X6-_N5tfVj_XlprGM0blhblCKt53kxlZjbhy4oVBLL7qOdUDVaMbRGcmVlFJ0RBKnXAVzyRgXjp-jt0fdXU5_FyiznnyxEIKJkJaieyl5z4mqQHEE2pxKyTDqXfbV_F5Tog-n0Iec9SFnLZW-P4XuK-3NSX8ZJnAPpFP2dX5xnEM1eesh62I9RAvO10hm7ZJ_fMF_E1WY5A</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Winter, Jane N.</creator><creator>Epstein, Alan L.</creator><creator>Bass, Bonny</creator><creator>Marder, Robert J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>The elimination of malignant B lymphocytes from human bone marrow using monoclonal antibodies DLC-48 and LN-1 and human serum: A preclinical study</title><author>Winter, Jane N. ; Epstein, Alan L. ; Bass, Bonny ; Marder, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c221t-2db9935683ac000dfb3a1eb3a746626e19faffda83988846080d9d3ac382234d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>autologous bone marrow transplantation</topic><topic>B-Lymphocytes - cytology</topic><topic>Blood</topic><topic>Bone Marrow Cells</topic><topic>Bone Marrow Transplantation</topic><topic>Cell Separation - methods</topic><topic>Humans</topic><topic>Lymphoma, Non-Hodgkin - pathology</topic><topic>marrow purging</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>non-Hodgkin's lymphoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Winter, Jane N.</creatorcontrib><creatorcontrib>Epstein, Alan L.</creatorcontrib><creatorcontrib>Bass, Bonny</creatorcontrib><creatorcontrib>Marder, Robert J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Winter, Jane N.</au><au>Epstein, Alan L.</au><au>Bass, Bonny</au><au>Marder, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The elimination of malignant B lymphocytes from human bone marrow using monoclonal antibodies DLC-48 and LN-1 and human serum: A preclinical study</atitle><jtitle>Leukemia research</jtitle><addtitle>Leuk Res</addtitle><date>1989</date><risdate>1989</risdate><volume>13</volume><issue>1</issue><spage>71</spage><epage>76</epage><pages>71-76</pages><issn>0145-2126</issn><eissn>1873-5835</eissn><abstract>Monoclonal antibodies DLC-48 and LN-1 were evaluated for use in purging malignant lymphoma cells from human bone marrow. Using a 51Cr-release assay and sensitive clonogenic assay for the SU-DHL-2 and -4 cell lines, it was established that greater than four logs of malignant lymphoid cells can be eliminated from human bone marrow autografts with three treatments of antibody and autologous human serum at a final cell concentration of 1 × 107cells/ml. A combination of DLC-48 and LN-1 was more effective than either antibody alone. Treatment with antibody and autologous serum had no effect on the growth of human hematopoietic progenitor cells. The clinical effects of marrow treatment with DLC-48 and LN-1 will be evaluated in upcoming clinical trials.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>2644495</pmid><doi>10.1016/0145-2126(89)90033-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-2126 |
| ispartof | Leukemia research, 1989, Vol.13 (1), p.71-76 |
| issn | 0145-2126 1873-5835 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78837309 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antibodies, Monoclonal autologous bone marrow transplantation B-Lymphocytes - cytology Blood Bone Marrow Cells Bone Marrow Transplantation Cell Separation - methods Humans Lymphoma, Non-Hodgkin - pathology marrow purging Mice Monoclonal antibodies non-Hodgkin's lymphoma |
| title | The elimination of malignant B lymphocytes from human bone marrow using monoclonal antibodies DLC-48 and LN-1 and human serum: A preclinical study |
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