Age-related decrease in an early step of DNA-repair of normal human lymphocytes exposed to ultraviolet-irradiation
A monoclonal antibody-based immunoassay has been used to detect age-related changes in the rate of loss of photoproduct antigenicity from the DNA of peripheral blood lymphocytes irradiated with 10 J m −2 uv-C. Lymphocytes were obtained from 75 healthy volunteers whose ages ranged from 14 months to 8...
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| Veröffentlicht in: | Exp. Cell Res.; (United States) 1989, Vol.180 (1), p.171-177 |
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| creator | Roth, M. Emmons, L.R. Häner, M. Müller, Hj Boyle, J.M. |
| description | A monoclonal antibody-based immunoassay has been used to detect age-related changes in the rate of loss of photoproduct antigenicity from the DNA of peripheral blood lymphocytes irradiated with 10 J m
−2 uv-C. Lymphocytes were obtained from 75 healthy volunteers whose ages ranged from 14 months to 82 years. The samples were divided by age decades into groups of 10 individuals, except the first decade which contained only 5 individuals. The mean loss of antigenicity ±1 standard deviation was determined for each group at 10, 30, and 60 min after irradiation. The data were analyzed by Mann-Whitney U test and by the Kruskal-Wallis test. After a recovery period of 10 min the loss of antigenicity was most rapid in group I (0–9 years), less rapid in group II (10–19 years), and least rapid in all other groups. The differences between groups became less at 30 min and were not significant at 60 min incubation. These data obtained from normal cells concur with our previous conclusions, that reductions in the rate of loss of antigenicity in nondiseased cells isolated from patients with melanoma and dysplastic naevus syndrome reflect genetic abnormalities in these patients. |
| doi_str_mv | 10.1016/0014-4827(89)90221-8 |
| format | Article |
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−2 uv-C. Lymphocytes were obtained from 75 healthy volunteers whose ages ranged from 14 months to 82 years. The samples were divided by age decades into groups of 10 individuals, except the first decade which contained only 5 individuals. The mean loss of antigenicity ±1 standard deviation was determined for each group at 10, 30, and 60 min after irradiation. The data were analyzed by Mann-Whitney U test and by the Kruskal-Wallis test. After a recovery period of 10 min the loss of antigenicity was most rapid in group I (0–9 years), less rapid in group II (10–19 years), and least rapid in all other groups. The differences between groups became less at 30 min and were not significant at 60 min incubation. These data obtained from normal cells concur with our previous conclusions, that reductions in the rate of loss of antigenicity in nondiseased cells isolated from patients with melanoma and dysplastic naevus syndrome reflect genetic abnormalities in these patients.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(89)90221-8</identifier><identifier>PMID: 2642424</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture ; Adolescent ; Adult ; AGE DEPENDENCE ; Aged ; Aged, 80 and over ; Aging ; ANIMAL CELLS ; ANIMALS ; ANTIBODIES ; ANTIGENS ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; BIOLOGICAL MATERIALS ; BIOLOGICAL RADIATION EFFECTS ; BIOLOGICAL RECOVERY ; BIOLOGICAL REPAIR ; BLOOD ; BLOOD CELLS ; BODY FLUIDS ; Child ; Child, Preschool ; CONNECTIVE TISSUE CELLS ; DNA ; DNA - analysis ; DNA - immunology ; DNA REPAIR ; ELECTROMAGNETIC RADIATION ; Fundamental and applied biological sciences. Psychology ; GENETIC EFFECTS ; GENETIC RADIATION EFFECTS ; Humans ; IMMUNOASSAY ; Immunoenzyme Techniques ; Infant ; LEUKOCYTES ; LYMPHOCYTES ; Lymphocytes - metabolism ; Lymphocytes - radiation effects ; MAMMALS ; MAN ; MATERIALS ; Middle Aged ; Molecular and cellular biology ; Molecular genetics ; MONOCLONAL ANTIBODIES ; Mutagenesis. Repair ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; PRIMATES ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RECOVERY ; REPAIR ; SOMATIC CELLS ; ULTRAVIOLET RADIATION ; Ultraviolet Rays ; VERTEBRATES</subject><ispartof>Exp. Cell Res.; (United States), 1989, Vol.180 (1), p.171-177</ispartof><rights>1989</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-849591a99d546fd551a468277b859e3b957f97c24a3e0cdb9980b428d5f34fd93</citedby><cites>FETCH-LOGICAL-c444t-849591a99d546fd551a468277b859e3b957f97c24a3e0cdb9980b428d5f34fd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014482789902218$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7256626$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2642424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6442095$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Roth, M.</creatorcontrib><creatorcontrib>Emmons, L.R.</creatorcontrib><creatorcontrib>Häner, M.</creatorcontrib><creatorcontrib>Müller, Hj</creatorcontrib><creatorcontrib>Boyle, J.M.</creatorcontrib><creatorcontrib>Kantonsspital, Basel (Switzerland)</creatorcontrib><title>Age-related decrease in an early step of DNA-repair of normal human lymphocytes exposed to ultraviolet-irradiation</title><title>Exp. Cell Res.; (United States)</title><addtitle>Exp Cell Res</addtitle><description>A monoclonal antibody-based immunoassay has been used to detect age-related changes in the rate of loss of photoproduct antigenicity from the DNA of peripheral blood lymphocytes irradiated with 10 J m
−2 uv-C. Lymphocytes were obtained from 75 healthy volunteers whose ages ranged from 14 months to 82 years. The samples were divided by age decades into groups of 10 individuals, except the first decade which contained only 5 individuals. The mean loss of antigenicity ±1 standard deviation was determined for each group at 10, 30, and 60 min after irradiation. The data were analyzed by Mann-Whitney U test and by the Kruskal-Wallis test. After a recovery period of 10 min the loss of antigenicity was most rapid in group I (0–9 years), less rapid in group II (10–19 years), and least rapid in all other groups. The differences between groups became less at 30 min and were not significant at 60 min incubation. These data obtained from normal cells concur with our previous conclusions, that reductions in the rate of loss of antigenicity in nondiseased cells isolated from patients with melanoma and dysplastic naevus syndrome reflect genetic abnormalities in these patients.</description><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</subject><subject>Adolescent</subject><subject>Adult</subject><subject>AGE DEPENDENCE</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Aging</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>ANTIBODIES</subject><subject>ANTIGENS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>BLOOD</subject><subject>BLOOD CELLS</subject><subject>BODY FLUIDS</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - immunology</subject><subject>DNA REPAIR</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETIC EFFECTS</subject><subject>GENETIC RADIATION EFFECTS</subject><subject>Humans</subject><subject>IMMUNOASSAY</subject><subject>Immunoenzyme Techniques</subject><subject>Infant</subject><subject>LEUKOCYTES</subject><subject>LYMPHOCYTES</subject><subject>Lymphocytes - metabolism</subject><subject>Lymphocytes - radiation effects</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>MATERIALS</subject><subject>Middle Aged</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>Mutagenesis. Repair</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>PRIMATES</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RECOVERY</subject><subject>REPAIR</subject><subject>SOMATIC CELLS</subject><subject>ULTRAVIOLET RADIATION</subject><subject>Ultraviolet Rays</subject><subject>VERTEBRATES</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U2PFCEQBmBiNOvs6j_QhBhj9NAKDHTDZZPJrl_JRi96JjRUOxi6aYHeOP9e2pnMUU-E1FMVihehZ5S8pYS27wihvOGSda-leqMIY7SRD9CGEkUaxhl7iDZn8hhd5vyTECIlbS_QBWt5JXyD0u4HNAmCKeCwA5vAZMB-wmbCYFI44FxgxnHAt192Fc7Gp_U2xTSagPfLWGE4jPM-2kOBjOH3HHOdVSJeQknm3scApfEpGedN8XF6gh4NJmR4ejqv0PcP77_dfGruvn78fLO7ayznvDSSK6GoUcoJ3g5OCGp4W1fpeikUbHslukF1lnGzBWJdr5QkPWfSiWHLB6e2V-jFcW7MxetsfQG7t3GawBbdcs6IEhW9OqI5xV8L5KJHny2EYCaIS9adlNuOye6_kArRScVJhfwIbYo5Jxj0nPxo0kFTotfg9JqKXlPRUum_wWlZ256f5i_9CO7cdEqq1l-e6iZbE4ZkJuvzmXVMtC1rK7s-Mqg_e-8hrYvDZMH5tO7tov_3O_4AYM6zsg</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Roth, M.</creator><creator>Emmons, L.R.</creator><creator>Häner, M.</creator><creator>Müller, Hj</creator><creator>Boyle, J.M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1989</creationdate><title>Age-related decrease in an early step of DNA-repair of normal human lymphocytes exposed to ultraviolet-irradiation</title><author>Roth, M. ; Emmons, L.R. ; Häner, M. ; Müller, Hj ; Boyle, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-849591a99d546fd551a468277b859e3b957f97c24a3e0cdb9980b428d5f34fd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</topic><topic>Adolescent</topic><topic>Adult</topic><topic>AGE DEPENDENCE</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Aging</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>ANTIBODIES</topic><topic>ANTIGENS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>BLOOD</topic><topic>BLOOD CELLS</topic><topic>BODY FLUIDS</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA - immunology</topic><topic>DNA REPAIR</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETIC EFFECTS</topic><topic>GENETIC RADIATION EFFECTS</topic><topic>Humans</topic><topic>IMMUNOASSAY</topic><topic>Immunoenzyme Techniques</topic><topic>Infant</topic><topic>LEUKOCYTES</topic><topic>LYMPHOCYTES</topic><topic>Lymphocytes - metabolism</topic><topic>Lymphocytes - radiation effects</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>MATERIALS</topic><topic>Middle Aged</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>Mutagenesis. Repair</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>PRIMATES</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>RECOVERY</topic><topic>REPAIR</topic><topic>SOMATIC CELLS</topic><topic>ULTRAVIOLET RADIATION</topic><topic>Ultraviolet Rays</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roth, M.</creatorcontrib><creatorcontrib>Emmons, L.R.</creatorcontrib><creatorcontrib>Häner, M.</creatorcontrib><creatorcontrib>Müller, Hj</creatorcontrib><creatorcontrib>Boyle, J.M.</creatorcontrib><creatorcontrib>Kantonsspital, Basel (Switzerland)</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Exp. Cell Res.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roth, M.</au><au>Emmons, L.R.</au><au>Häner, M.</au><au>Müller, Hj</au><au>Boyle, J.M.</au><aucorp>Kantonsspital, Basel (Switzerland)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-related decrease in an early step of DNA-repair of normal human lymphocytes exposed to ultraviolet-irradiation</atitle><jtitle>Exp. Cell Res.; (United States)</jtitle><addtitle>Exp Cell Res</addtitle><date>1989</date><risdate>1989</risdate><volume>180</volume><issue>1</issue><spage>171</spage><epage>177</epage><pages>171-177</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>A monoclonal antibody-based immunoassay has been used to detect age-related changes in the rate of loss of photoproduct antigenicity from the DNA of peripheral blood lymphocytes irradiated with 10 J m
−2 uv-C. Lymphocytes were obtained from 75 healthy volunteers whose ages ranged from 14 months to 82 years. The samples were divided by age decades into groups of 10 individuals, except the first decade which contained only 5 individuals. The mean loss of antigenicity ±1 standard deviation was determined for each group at 10, 30, and 60 min after irradiation. The data were analyzed by Mann-Whitney U test and by the Kruskal-Wallis test. After a recovery period of 10 min the loss of antigenicity was most rapid in group I (0–9 years), less rapid in group II (10–19 years), and least rapid in all other groups. The differences between groups became less at 30 min and were not significant at 60 min incubation. These data obtained from normal cells concur with our previous conclusions, that reductions in the rate of loss of antigenicity in nondiseased cells isolated from patients with melanoma and dysplastic naevus syndrome reflect genetic abnormalities in these patients.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>2642424</pmid><doi>10.1016/0014-4827(89)90221-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Exp. Cell Res.; (United States), 1989, Vol.180 (1), p.171-177 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| subjects | 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture Adolescent Adult AGE DEPENDENCE Aged Aged, 80 and over Aging ANIMAL CELLS ANIMALS ANTIBODIES ANTIGENS Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGICAL MATERIALS BIOLOGICAL RADIATION EFFECTS BIOLOGICAL RECOVERY BIOLOGICAL REPAIR BLOOD BLOOD CELLS BODY FLUIDS Child Child, Preschool CONNECTIVE TISSUE CELLS DNA DNA - analysis DNA - immunology DNA REPAIR ELECTROMAGNETIC RADIATION Fundamental and applied biological sciences. Psychology GENETIC EFFECTS GENETIC RADIATION EFFECTS Humans IMMUNOASSAY Immunoenzyme Techniques Infant LEUKOCYTES LYMPHOCYTES Lymphocytes - metabolism Lymphocytes - radiation effects MAMMALS MAN MATERIALS Middle Aged Molecular and cellular biology Molecular genetics MONOCLONAL ANTIBODIES Mutagenesis. Repair NUCLEIC ACIDS ORGANIC COMPOUNDS PRIMATES RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RECOVERY REPAIR SOMATIC CELLS ULTRAVIOLET RADIATION Ultraviolet Rays VERTEBRATES |
| title | Age-related decrease in an early step of DNA-repair of normal human lymphocytes exposed to ultraviolet-irradiation |
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