Quantitation of a cationic antimicrobial granule protein of human polymorphonuclear leukocytes by ELISA

The quantitation of CAP57, a highly hydrophobic, native cationic antigen of human polymorphonuclear leukocytes has been achieved using ELISA. An important feature determining the sensitivity and precision of the ELISA was the reduction of non-specific protein-protein binding, particularly in the inh...

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Veröffentlicht in:	Journal of immunological methods 1989-02, Vol.117 (1), p.115-120
Hauptverfasser:	Pereira, H.Anne, Martin, Larry E., Spitznagel, John K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antigen-Antibody Reactions Antimicrobial Cationic Peptides Biological and medical sciences Blood Bactericidal Activity Blood Proteins - analysis Blood Proteins - immunology Cationic antimicrobial protein Cytoplasmic Granules - analysis Cytoplasmic Granules - immunology ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Leukemia, Myelogenous, Chronic, BCR-ABL Positive - blood Membrane Proteins Mice Mice, Inbred BALB C Myeloid cells: ontogeny, maturation, markers, receptors Neutrophils - analysis Neutrophils - immunology Non-specific binding Polymorphonuclear leukocyte Polynuclears
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